SRR2934030.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934030.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135426
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	5654	4.174973786422105	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	5533	4.085626098385834	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	3537	2.6117584511098313	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT	2373	1.7522484604138053	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1341	0.9902086748482567	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA	1244	0.9185828422902544	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	1072	0.7915762113626631	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	1019	0.7524404471814866	No Hit
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	967	0.714043093645238	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	816	0.6025430862611316	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	561	0.41424837180452795	TruSeq Adapter, Index 22 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	487	0.3596059840798665	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	426	0.3145629347392672	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGT	395	0.2916722047465036	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATG	334	0.24662915540590433	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTAT	322	0.23776822766677003	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAAGGTCC	315	0.23259935315227503	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	257	0.18977153574645933	No Hit
TGCATGGGGATAAACCTGAAATAGAAGATGTTGGCTCTGATGAAGAAGAGG	251	0.18534107187689217	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTCGAATGC	239	0.1764801441377579	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	234	0.1727880909131186	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACCTCTGACGCAAAGGTCCT	205	0.15137418221021073	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	175	0.12922186286237503	No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	155	0.11445364996381788	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	149	0.11002318609425073	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGGTCCTCGTATGC	143	0.10559272222468359	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	141	0.10411590093482787	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGCG	20	7.011233E-4	45.000004	27
TGGAGCG	20	7.011233E-4	45.000004	22
GTCGCGG	25	3.8725375E-5	45.000004	2
AGTCCGG	20	7.011233E-4	45.000004	11
CGAACAC	55	1.8189894E-12	45.000004	34
AATCAGT	20	7.011233E-4	45.000004	16
ACTGAGC	20	7.011233E-4	45.000004	12
CGACGAA	25	3.8725375E-5	45.000004	19
GAGTCCG	20	7.011233E-4	45.000004	10
GCGAACA	55	1.8189894E-12	45.000004	33
GGCACCG	50	2.1827873E-11	45.000004	8
GCAACTA	20	7.011233E-4	45.000004	7
TGGGTTT	20	7.011233E-4	45.000004	6
GTCCTCA	20	7.011233E-4	45.000004	17
TTATGTC	25	3.8725375E-5	45.000004	8
CACATAG	20	7.011233E-4	45.000004	34
GATCCCT	45	3.8016879E-10	45.000004	5
GCAAAAC	25	3.8725375E-5	45.000004	9
CGACCCT	20	7.011233E-4	45.000004	12
CGACCCG	20	7.011233E-4	45.000004	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content