SRR2934029.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242329
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1686	0.6957483421299143	RNA PCR Primer, Index 41 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1383	0.5707117183663531	RNA PCR Primer, Index 41 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	1025	0.4229786777480203	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	880	0.36314266967634906	No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	830	0.3425095634447384	Illumina PCR Primer Index 11 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	746	0.3078459449756323	Illumina PCR Primer Index 11 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	590	0.24347065353300676	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGACATCT	372	0.15351031036318394	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	362	0.14938368911686178	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.1283379207606188	No Hit
TTGTTTTTTTAAACAAAGACTCGTAGAGATGAAAAAATTACTAATGAAATT	280	0.11554539489702018	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	274	0.11306942214922688	RNA PCR Primer, Index 41 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	265	0.10935546302753694	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAC	20	7.0222584E-4	45.0	31
CTTCGTG	25	3.8816666E-5	45.0	1
TCGTTTG	70	0.0	45.0	1
CGTGAAC	20	7.0222584E-4	45.0	33
GATCCTA	20	7.0222584E-4	45.0	17
TCTTACT	20	7.0222584E-4	45.0	20
TTCCGAT	20	7.0222584E-4	45.0	13
GCACCGA	40	6.7775545E-9	45.0	9
TCGGAAT	20	7.0222584E-4	45.0	5
GGGACGA	20	7.0222584E-4	45.0	7
CTAGTTG	20	7.0222584E-4	45.0	1
GTTCGGA	20	7.0222584E-4	45.0	3
AAACAGG	45	3.8198777E-10	45.0	17
GCCTATA	45	3.8198777E-10	45.0	1
GGTGCAA	20	7.0222584E-4	45.0	8
CTCGAAC	20	7.0222584E-4	45.0	12
GGGCAAC	35	1.2066266E-7	45.0	7
CGGTAGT	45	3.8198777E-10	45.0	12
CGCCGGT	45	3.8198777E-10	45.0	28
ACATACG	50	2.1827873E-11	45.0	17