##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242329
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.025275555133724	31.0	30.0	31.0	26.0	34.0
2	30.253807839755044	31.0	30.0	33.0	26.0	34.0
3	30.28610277762876	31.0	30.0	34.0	26.0	34.0
4	33.99030244007114	35.0	33.0	37.0	28.0	37.0
5	34.42084934118492	35.0	35.0	37.0	32.0	37.0
6	33.91308922993121	35.0	35.0	37.0	30.0	37.0
7	34.389354967832986	35.0	35.0	37.0	32.0	37.0
8	34.45836858155648	35.0	35.0	37.0	32.0	37.0
9	35.84640715721189	37.0	35.0	39.0	30.0	39.0
10	35.28420040523421	37.0	34.0	39.0	30.0	39.0
11	35.380829368338084	37.0	35.0	39.0	30.0	39.0
12	35.33286152297084	37.0	34.0	39.0	30.0	39.0
13	35.19115334937214	37.0	34.0	39.0	29.0	39.0
14	35.85646373318918	38.0	34.0	40.0	28.0	41.0
15	36.12603526610517	38.0	34.0	40.0	30.0	41.0
16	36.32980782324856	38.0	35.0	40.0	31.0	41.0
17	36.1284163265643	38.0	34.0	40.0	30.0	41.0
18	36.2310165106116	38.0	35.0	40.0	30.0	41.0
19	36.23128474099262	38.0	34.0	40.0	30.0	41.0
20	36.27755241840638	38.0	34.0	40.0	30.0	41.0
21	35.947406212215625	38.0	34.0	40.0	29.0	41.0
22	36.15215677859439	38.0	34.0	40.0	30.0	41.0
23	36.267677413763934	38.0	34.0	40.0	30.0	41.0
24	36.152350729792964	38.0	34.0	40.0	30.0	41.0
25	35.70864403352467	38.0	34.0	40.0	29.0	41.0
26	35.78250642721259	38.0	34.0	40.0	29.0	41.0
27	35.822423234528266	38.0	34.0	40.0	30.0	41.0
28	35.701245001630014	38.0	34.0	40.0	29.0	41.0
29	35.816992600968106	38.0	34.0	40.0	29.0	41.0
30	35.383255821630925	38.0	34.0	40.0	28.0	41.0
31	35.519508601941986	38.0	34.0	40.0	29.0	41.0
32	35.59313165159762	38.0	34.0	40.0	29.0	41.0
33	35.62504694031668	38.0	34.0	40.0	29.0	41.0
34	35.57037333542416	38.0	34.0	40.0	29.0	41.0
35	35.601083650739284	38.0	34.0	40.0	29.0	41.0
36	35.43700506336427	38.0	34.0	40.0	28.0	41.0
37	35.48289721824462	38.0	34.0	40.0	29.0	41.0
38	35.37777154199456	38.0	34.0	40.0	28.0	41.0
39	35.35173668855151	37.0	34.0	40.0	28.0	41.0
40	35.26643530076879	37.0	34.0	40.0	28.0	41.0
41	35.15998085247742	37.0	34.0	40.0	27.0	41.0
42	35.4248562904151	37.0	34.0	40.0	29.0	41.0
43	35.42786459730367	37.0	34.0	40.0	29.0	41.0
44	35.352846749666774	37.0	34.0	40.0	28.0	41.0
45	35.42467059245901	37.0	34.0	40.0	29.0	41.0
46	35.308559850451246	37.0	34.0	40.0	28.0	41.0
47	35.200665211344905	37.0	34.0	40.0	28.0	41.0
48	35.20405729400938	37.0	34.0	40.0	28.0	41.0
49	35.277874294863594	37.0	34.0	40.0	28.0	41.0
50	35.08958069401516	37.0	34.0	40.0	28.0	41.0
51	33.95045165869541	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	7.0
12	5.0
13	4.0
14	8.0
15	14.0
16	33.0
17	50.0
18	98.0
19	173.0
20	325.0
21	540.0
22	768.0
23	1124.0
24	1496.0
25	2121.0
26	2723.0
27	3655.0
28	4661.0
29	6046.0
30	7850.0
31	10123.0
32	12836.0
33	17172.0
34	23679.0
35	23906.0
36	28178.0
37	35080.0
38	41126.0
39	18522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.226514366831868	4.649876820355797	56.10182850587424	18.021780306938087
2	20.135848371428924	5.6179821647429735	53.53094346941555	20.715225994412556
3	21.018945318141867	5.6014756797576855	52.09033999232449	21.289239009775965
4	20.359511242979586	4.945755563717095	48.32356011868163	26.37117307462169
5	18.22357208588324	5.726924965645878	49.98411250820166	26.06539044026922
6	22.005207796012858	7.589269134111063	52.09859323481712	18.30692983505895
7	80.55082140395908	2.940217638004531	11.769123794510769	4.73983716352562
8	81.82553470694799	4.309843229658853	7.95942705990616	5.905195003486995
9	75.81799949655222	5.303533625773225	11.128259514956937	7.750207362717627
10	42.366782349615605	24.841434578610073	18.27515485146227	14.516628220312056
11	31.119263480639958	26.12481378621626	24.06769309492467	18.688229638219113
12	28.59954854763565	21.073416718593318	29.424872796900083	20.902161936870947
13	24.507178257657976	25.849568149086572	30.444148244741655	19.199105348513797
14	19.64065382187027	27.637220472993327	30.098750046424488	22.623375658711918
15	17.637591868905496	25.910642143532144	35.576839750917145	20.874926236645223
16	19.962530279083396	24.66440252714285	32.6774756632512	22.695591530522556
17	20.431314452665593	24.323956274321272	29.833408300285974	25.411320972727157
18	21.402308431925192	23.89066104345745	31.50345191867255	23.20357860594481
19	23.499044687181474	25.916832075401626	29.401763717920677	21.18235951949622
20	25.454237833688907	26.046820644660773	29.774397616463567	18.72454390518675
21	24.072645040420255	27.456061800279784	29.184290778239504	19.287002381060457
22	21.879345848000035	24.541429214002452	29.268886513789106	24.31033842420841
23	22.127768447028625	26.034440780921802	28.512889501462883	23.324901270586683
24	22.448406917867857	23.91500810881075	30.053769874839574	23.582815098481817
25	21.41468829566416	25.516137152383745	28.207519529235046	24.86165502271705
26	20.102010077209084	26.917537727634745	28.988688931163832	23.99176326399234
27	20.750714937130926	27.0578428500097	28.884285413631883	23.307156799227496
28	18.67419912598162	27.13872462643761	31.476216218446822	22.710860029133944
29	19.655509658357026	26.79415175236971	29.846613488274205	23.703725100999055
30	20.611235139005238	26.46319672841468	29.06296811359763	23.86260001898246
31	22.07989964057129	28.811656879696613	26.112021260352662	22.99642221937944
32	22.32832223959988	28.88511073788115	26.25315170697688	22.533415315542094
33	21.78402089720999	26.53252396535289	25.68367797498442	25.9997771624527
34	20.19774769012376	26.298131878561794	29.353482249338708	24.150638181975744
35	19.657985631104822	25.20952919378201	29.215240437586914	25.917244737526257
36	20.867085656277208	26.517255466741496	27.81136388958812	24.80429498739317
37	19.63776518699784	29.312216036875487	28.08372089184538	22.966297884281285
38	20.79445712234194	28.629260220609172	26.133892352958167	24.44239030409072
39	20.197335027999124	27.54272084645255	27.981380684936596	24.27856344061173
40	20.6747851061986	25.07046205778095	28.88057145451019	25.37418138151026
41	17.49274746315959	26.100466720862958	29.05388954685572	27.35289626912173
42	19.8127339278419	24.937584853649376	28.9870382826653	26.262642935843424
43	20.94095217658638	23.754069880204185	27.678899347581183	27.626078595628258
44	19.80365536109999	24.497274366666804	28.53599858044229	27.163071691790915
45	20.450296910398674	24.934696218776953	27.236112887850815	27.378893982973562
46	21.395293175806447	25.51201053113742	28.876032171139236	24.2166641219169
47	17.593849683694483	26.18506245641256	30.53287060153758	25.68821725835538
48	18.38739894936223	24.667291162015275	30.946770712543692	25.9985391760788
49	20.193621068877434	23.19986464682312	31.313627341341732	25.292886942957715
50	19.703791126938995	23.481712877946922	29.18222746761634	27.63226852749774
51	18.22357208588324	24.457246140577478	27.99953781842041	29.319643955118867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	263.5
2	385.0
3	527.5
4	670.0
5	503.5
6	337.0
7	330.5
8	324.0
9	387.5
10	451.0
11	508.5
12	566.0
13	601.0
14	636.0
15	582.0
16	528.0
17	504.5
18	481.0
19	588.0
20	695.0
21	982.0
22	1269.0
23	1221.0
24	1173.0
25	1355.5
26	1973.5
27	2409.0
28	2947.0
29	3485.0
30	3768.0
31	4051.0
32	4652.5
33	5254.0
34	5881.5
35	6509.0
36	6947.5
37	7386.0
38	7966.5
39	8547.0
40	10225.5
41	11904.0
42	13689.5
43	15475.0
44	16946.0
45	18417.0
46	21613.0
47	24809.0
48	25647.0
49	26485.0
50	25541.0
51	24597.0
52	20638.0
53	16679.0
54	14881.5
55	13084.0
56	11442.0
57	9800.0
58	9466.0
59	9132.0
60	8400.5
61	7669.0
62	6806.5
63	5944.0
64	4954.5
65	3965.0
66	3476.5
67	2988.0
68	2448.0
69	1908.0
70	1428.5
71	949.0
72	837.5
73	726.0
74	613.0
75	377.0
76	254.0
77	187.0
78	120.0
79	80.0
80	40.0
81	41.5
82	43.0
83	23.5
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	242329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.08517346252409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01325948650374	29.66834345043309
2	10.113446848813028	8.107985424773757
3	3.7070971195618605	4.457988932401817
4	2.050690769832609	3.2880918090694884
5	1.3341843562766373	2.6740505676167525
6	1.056229282052338	2.540348039235915
7	0.9151928184644526	2.567996401586273
8	0.8256295167699561	2.6476401916402907
9	0.6876814428956742	2.480924693288876
>10	5.182317939426383	34.25013102022457
>50	0.07412135312647986	2.007188574211093
>100	0.03294282361176882	2.362903325644062
>500	0.004117852951471102	1.2569688316297265
>1k	0.003088389713603327	1.6894387382442877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1686	0.6957483421299143	RNA PCR Primer, Index 41 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1383	0.5707117183663531	RNA PCR Primer, Index 41 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	1025	0.4229786777480203	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	880	0.36314266967634906	No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	830	0.3425095634447384	Illumina PCR Primer Index 11 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	746	0.3078459449756323	Illumina PCR Primer Index 11 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	590	0.24347065353300676	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGACATCT	372	0.15351031036318394	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	362	0.14938368911686178	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.1283379207606188	No Hit
TTGTTTTTTTAAACAAAGACTCGTAGAGATGAAAAAATTACTAATGAAATT	280	0.11554539489702018	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	274	0.11306942214922688	RNA PCR Primer, Index 41 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	265	0.10935546302753694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6309603885626566	0.0
2	0.0	0.0	0.0	2.141303764716563	0.0
3	0.0	0.0	0.0	2.6513541507619807	0.0
4	0.0	0.0	0.0	3.4828683318958937	0.0
5	0.0	0.0	0.0	4.878078975277412	0.0
6	0.0	0.0	0.0	5.533386429193369	0.0
7	0.0	0.0	0.0	6.229960095572548	0.0
8	0.0	0.0	0.0	7.265742028399408	0.0
9	0.0	0.0	0.0	7.6771661666577256	0.0
10	0.0	0.0	0.0	8.866045747723136	0.0
11	0.0	0.0	0.0	10.611606534917406	0.0
12	0.0	0.0	0.0	12.641078863858638	0.0
13	0.0	0.0	0.0	13.17630163950662	0.0
14	0.0	0.0	0.0	13.404091132303604	0.0
15	0.0	0.0	0.0	13.804373393196853	0.0
16	0.0	0.0	0.0	14.553767811528955	0.0
17	0.0	0.0	0.0	15.553648139512811	0.0
18	0.0	0.0	0.0	16.761922840435936	0.0
19	0.0	0.0	0.0	17.708157092217604	0.0
20	0.0	0.0	0.0	18.539258611226884	0.0
21	0.0	0.0	0.0	19.629924606629828	0.0
22	0.0	0.0	0.0	20.700370157925796	0.0
23	0.0	0.0	0.0	21.743167346871402	0.0
24	0.0	0.0	0.0	22.57715750075311	0.0
25	4.1266212463221487E-4	0.0	0.0	23.338106458574913	0.0
26	4.1266212463221487E-4	0.0	0.0	24.046234664443794	0.0
27	4.1266212463221487E-4	0.0	0.0	24.67884570150498	0.0
28	4.1266212463221487E-4	0.0	0.0	25.37583203000879	0.0
29	4.1266212463221487E-4	0.0	0.0	26.086436208625464	0.0
30	4.1266212463221487E-4	0.0	0.0	26.870906907551305	0.0
31	4.1266212463221487E-4	0.0	0.0	27.597192246904	0.0
32	4.1266212463221487E-4	0.0	0.0	28.33255615299861	0.0
33	4.1266212463221487E-4	0.0	0.0	29.041509683116754	0.0
34	4.1266212463221487E-4	0.0	0.0	29.70011843402977	0.0
35	4.1266212463221487E-4	0.0	0.0	30.475923228338335	0.0
36	4.1266212463221487E-4	0.0	0.0	31.19643129794618	0.0
37	4.1266212463221487E-4	0.0	0.0	31.902908855316532	0.0
38	4.1266212463221487E-4	0.0	0.0	32.6531285978979	0.0
39	4.1266212463221487E-4	0.0	0.0	33.41490287996897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAC	20	7.0222584E-4	45.0	31
CTTCGTG	25	3.8816666E-5	45.0	1
TCGTTTG	70	0.0	45.0	1
CGTGAAC	20	7.0222584E-4	45.0	33
GATCCTA	20	7.0222584E-4	45.0	17
TCTTACT	20	7.0222584E-4	45.0	20
TTCCGAT	20	7.0222584E-4	45.0	13
GCACCGA	40	6.7775545E-9	45.0	9
TCGGAAT	20	7.0222584E-4	45.0	5
GGGACGA	20	7.0222584E-4	45.0	7
CTAGTTG	20	7.0222584E-4	45.0	1
GTTCGGA	20	7.0222584E-4	45.0	3
AAACAGG	45	3.8198777E-10	45.0	17
GCCTATA	45	3.8198777E-10	45.0	1
GGTGCAA	20	7.0222584E-4	45.0	8
CTCGAAC	20	7.0222584E-4	45.0	12
GGGCAAC	35	1.2066266E-7	45.0	7
CGGTAGT	45	3.8198777E-10	45.0	12
CGCCGGT	45	3.8198777E-10	45.0	28
ACATACG	50	2.1827873E-11	45.0	17
>>END_MODULE