FastQCFastQC Report
Sat 14 Jan 2017
SRR2934028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563611
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC83091.4742437603240532No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG68781.2203452381163604No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC64981.1529228492701526No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA35370.627560498286939No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTAAATT33240.5897684750652489No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT22930.40684088848514316No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTA18970.33657966221383184No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT17850.31670780023810746No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC16140.286367725257314No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTC15490.2748349482178311No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT12420.22036475512365797No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT9750.17299165559224358No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC8600.1525875115993123No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCGC6860.1217151546013119No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGT6840.12136029992317397No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATG6490.1151503430557601No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC6470.11479548837762216No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTA6250.11089208691810486No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC6230.11053723223996693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA302.1635242E-645.00000441
ACCGCGT302.1635242E-645.00000439
TACGGAG650.045.00000429
ACGATCG351.2104465E-745.00000440
GCCGAAT302.1635242E-645.00000442
GTCGTCT253.888266E-545.00000411
GCGATAG302.1635242E-645.0000049
CCGCGTA302.1635242E-645.00000440
ATACGCC253.888266E-545.00000428
TACCGGT302.1635242E-645.00000440
TATTTCG207.030225E-445.01
GTCATCG207.030225E-445.033
TACGGTC207.030225E-445.09
ATTCCGT207.030225E-445.037
GCGATAA207.030225E-445.033
TTACGCG207.030225E-445.01
TCATACG207.030225E-445.06
CGATCGA1550.045.041
ATCGAAT2350.044.04255343
CATTAGA10400.043.26923417
Produced by FastQC (version 0.11.5)