##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934028.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563611
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.55988083979908	31.0	28.0	31.0	25.0	33.0
2	29.140730042529334	31.0	30.0	33.0	16.0	34.0
3	29.2924304174333	31.0	28.0	33.0	16.0	34.0
4	33.62983689104719	35.0	32.0	37.0	28.0	37.0
5	34.254187728770376	35.0	35.0	37.0	32.0	37.0
6	33.111339203812555	35.0	33.0	37.0	28.0	37.0
7	34.182817581629884	35.0	35.0	36.0	32.0	37.0
8	33.512986794083155	35.0	35.0	37.0	28.0	37.0
9	35.477345190210976	37.0	34.0	39.0	30.0	39.0
10	35.1658537537415	37.0	34.0	39.0	30.0	39.0
11	35.339102678975394	37.0	35.0	39.0	30.0	39.0
12	35.29922056169947	37.0	34.0	39.0	30.0	39.0
13	35.16733349774933	37.0	34.0	39.0	29.0	39.0
14	35.940886533442395	38.0	34.0	40.0	28.0	41.0
15	36.11507227502657	38.0	34.0	40.0	30.0	41.0
16	36.41126415204813	38.0	35.0	40.0	31.0	41.0
17	36.00026081818843	38.0	34.0	40.0	29.0	41.0
18	36.16702477417935	38.0	35.0	40.0	30.0	41.0
19	36.134113777055454	38.0	34.0	40.0	30.0	41.0
20	35.88659021914051	38.0	34.0	40.0	29.0	41.0
21	35.796166150057395	38.0	34.0	40.0	29.0	41.0
22	35.960164013832234	38.0	34.0	40.0	30.0	41.0
23	35.76070197352429	38.0	34.0	40.0	29.0	41.0
24	35.765834946443555	38.0	34.0	40.0	29.0	41.0
25	35.56744456726359	38.0	34.0	40.0	29.0	41.0
26	35.600944623153204	38.0	34.0	40.0	29.0	41.0
27	35.40456804427167	38.0	34.0	40.0	27.0	41.0
28	35.26119433439021	38.0	34.0	40.0	27.0	41.0
29	35.46177416693428	38.0	34.0	40.0	27.0	41.0
30	35.089849204504524	37.0	33.0	40.0	27.0	41.0
31	35.332360440090774	38.0	34.0	40.0	27.0	41.0
32	34.983169242615915	37.0	33.0	40.0	26.0	41.0
33	34.88716153517231	37.0	33.0	40.0	25.0	41.0
34	34.86117730136566	38.0	33.0	40.0	25.0	41.0
35	34.81266511831742	38.0	33.0	40.0	25.0	41.0
36	34.533518685760214	37.0	33.0	40.0	24.0	41.0
37	34.497421093626635	37.0	33.0	40.0	24.0	41.0
38	34.58398789235838	37.0	33.0	40.0	24.0	41.0
39	34.338937671550056	37.0	33.0	40.0	24.0	41.0
40	34.21615263009416	37.0	33.0	40.0	24.0	41.0
41	34.11058513762151	37.0	32.0	40.0	23.0	41.0
42	34.152887363802336	37.0	33.0	40.0	23.0	41.0
43	34.17575952208172	37.0	33.0	40.0	23.0	41.0
44	34.33174121867742	37.0	33.0	40.0	24.0	41.0
45	34.264868854582325	37.0	33.0	40.0	24.0	41.0
46	34.16668411368834	37.0	33.0	40.0	23.0	41.0
47	34.052715436710784	36.0	33.0	40.0	23.0	41.0
48	34.023860428557995	36.0	33.0	40.0	23.0	41.0
49	34.051540867726146	36.0	33.0	40.0	24.0	41.0
50	33.82044353286221	36.0	33.0	40.0	23.0	41.0
51	32.932096783064914	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	7.0
12	9.0
13	20.0
14	12.0
15	31.0
16	61.0
17	156.0
18	358.0
19	939.0
20	2028.0
21	3582.0
22	4986.0
23	6702.0
24	7783.0
25	8274.0
26	8742.0
27	9765.0
28	11883.0
29	14754.0
30	18976.0
31	23874.0
32	30309.0
33	38663.0
34	51320.0
35	53656.0
36	63013.0
37	77011.0
38	86841.0
39	39844.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.75641887755917	4.062731210001224	45.57132490316903	15.609525009270579
2	17.367120230087775	15.91150634036596	45.69587889519545	21.02549453435082
3	19.463779095865767	16.069771526815483	45.464336217710446	19.00211315960831
4	17.918032117896917	4.955900434874408	50.784849834371585	26.341217612857093
5	26.650828319532444	7.471110393516095	42.34161505009661	23.536446236854854
6	19.716435626699976	18.324340724364856	45.196420935716304	16.762802713218868
7	73.6782993944405	2.4916121225455146	17.96611492678461	5.863973556229385
8	73.67457342032004	12.517498771315678	8.942337889075976	4.865589919288303
9	68.76533637562078	5.037517010846133	9.81332869656554	16.383817916967555
10	47.39226168403385	22.850512144014225	14.918445523596949	14.838780648354982
11	36.04578335057336	23.70908303776896	22.001522326569212	18.243611285088473
12	25.291380047586014	19.350935308217903	27.131124126392137	28.226560517803946
13	27.769862547040425	20.9724437599692	29.96854213278307	21.289151560207305
14	20.744981911282782	29.52018324695579	26.800222139028513	22.934612702732913
15	17.633083811352158	20.52674628422795	35.932584708247354	25.90758519617254
16	21.600004258256135	22.511803353731562	27.979581661819942	27.908610726192357
17	21.232907093722446	27.00532814299224	27.83959149129453	23.922173271990786
18	21.31807221647555	23.326017412719054	28.467329416920535	26.888580953884862
19	22.153045274134108	23.974336909677067	26.948728821829242	26.923888994359586
20	29.309576995480924	23.684243210299304	29.64580180301662	17.36037799120315
21	24.512119174395107	31.688345330378574	25.223780231400735	18.575755263825585
22	24.395549412626792	21.01697802207551	31.67539313462654	22.912079430671152
23	25.741158352125847	27.21841837721407	26.764204389197516	20.27621888146257
24	22.710876828166946	28.00938945478353	26.442883478143614	22.83685023890591
25	20.180230691026257	33.1753638591156	23.932996339673995	22.711409110184153
26	20.370255371169122	23.7380036940372	31.05138118312098	24.840359751672697
27	25.486017838544672	24.5167322852109	26.631133884895785	23.366115991348643
28	18.957046615484792	28.589399426199986	29.12327828945851	23.33027566885671
29	20.317736878804705	23.69506627798251	30.794643823488187	25.192553019724595
30	20.81417857351968	30.485033116812836	27.559788577582765	21.140999732084715
31	24.333627271291725	23.31004895220285	23.47505637753699	28.881267398968436
32	26.56903431622165	27.346520916021866	24.49632814121797	21.58811662653852
33	25.456210045581084	24.74383927921918	23.24493311876454	26.5550175564352
34	21.123256998177823	25.450532370730876	26.03089719682547	27.395313434265834
35	26.122627131124126	25.366076957334048	26.291360530578718	22.21993538096311
36	20.6796886505054	28.154702445481018	25.83111401303381	25.33449489097977
37	22.18249821241956	25.365367247977773	27.56085314161718	24.89128139798549
38	20.201699399053602	27.389103477398418	23.82228168009496	28.58691544345302
39	20.69867337578578	29.409823442054893	27.35805369306135	22.533449489097976
40	21.13532205723451	26.79862529297689	26.94376085633531	25.12229179345329
41	18.32664727977275	27.22516061609869	26.31247438392792	28.13571772020064
42	22.42113798346732	24.354918551980003	27.96787145744139	25.256072007111285
43	20.618653645865674	25.21295716371753	27.601129147585834	26.56726004283096
44	22.40073383947439	23.503089897109884	29.36209548784534	24.734080775570387
45	21.592197455337104	23.571931704668643	26.05804357970302	28.77782726029123
46	23.891478342331858	26.696604573012237	27.014554364623827	22.39736272003208
47	18.45262069051172	27.779975905367355	31.390444828081783	22.376958576039147
48	19.96323705534491	27.32487478065545	27.680084313471525	25.03180385052811
49	21.624489231047654	23.5887873018802	31.370572966106053	23.416150500966094
50	21.52424278447369	25.50819625592829	28.498201773918534	24.469359185679483
51	19.71767761807346	23.379245614439746	27.711488952486736	29.191587815000062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	233.5
2	394.0
3	768.5
4	1143.0
5	898.0
6	653.0
7	634.5
8	616.0
9	674.5
10	733.0
11	769.0
12	805.0
13	810.5
14	816.0
15	858.0
16	900.0
17	936.5
18	973.0
19	1028.0
20	1083.0
21	1185.5
22	1288.0
23	1659.5
24	2031.0
25	2385.0
26	3490.5
27	4242.0
28	4946.0
29	5650.0
30	8474.0
31	11298.0
32	11047.5
33	10797.0
34	12520.5
35	14244.0
36	14989.0
37	15734.0
38	18302.5
39	20871.0
40	22760.5
41	24650.0
42	27171.0
43	29692.0
44	47618.5
45	65545.0
46	58163.0
47	50781.0
48	49199.5
49	47618.0
50	44042.0
51	40466.0
52	38820.5
53	37175.0
54	35089.5
55	33004.0
56	31047.5
57	29091.0
58	27440.0
59	25789.0
60	24239.0
61	22689.0
62	20474.0
63	18259.0
64	16570.5
65	14882.0
66	12592.5
67	10303.0
68	8489.0
69	6675.0
70	5471.0
71	4267.0
72	3305.5
73	2344.0
74	1930.0
75	1273.5
76	1031.0
77	718.5
78	406.0
79	317.0
80	228.0
81	155.5
82	83.0
83	47.0
84	11.0
85	15.0
86	19.0
87	10.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.319235557594887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.58492308240052	22.613578519803976
2	8.857252864281412	5.370902719706602
3	3.151016348164694	2.8660922071751327
4	1.6013553518834207	1.9420748050067473
5	1.1053501431845918	1.6756685682417436
6	0.8290074891105593	1.5080924004811995
7	0.6909104975988433	1.4663514688140096
8	0.6337245629791572	1.5371235442879154
9	0.5889369024392704	1.6070505006254867
>10	7.77351404867135	45.62212879306256
>50	0.12168317872148982	2.5461851653020826
>100	0.04986042445173241	3.009242447626246
>500	0.005935764815682429	1.2300802661040242
>1k	0.004748611852545944	3.1028257304900486
>5k	0.0017807294447047288	3.9026028632722407
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	8309	1.4742437603240532	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	6878	1.2203452381163604	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	6498	1.1529228492701526	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	3537	0.627560498286939	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTAAATT	3324	0.5897684750652489	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	2293	0.40684088848514316	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTA	1897	0.33657966221383184	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	1785	0.31670780023810746	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1614	0.286367725257314	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTC	1549	0.2748349482178311	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	1242	0.22036475512365797	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	975	0.17299165559224358	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	860	0.1525875115993123	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCGC	686	0.1217151546013119	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGT	684	0.12136029992317397	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATG	649	0.1151503430557601	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	647	0.11479548837762216	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTA	625	0.11089208691810486	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	623	0.11053723223996693	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.192863517567968	0.0
2	0.0	0.0	0.0	0.8229079986018726	0.0
3	0.0	0.0	0.0	1.0205620543247027	0.0
4	0.0	0.0	0.0	1.6793497642877802	0.0
5	0.0	0.0	0.0	3.5499662000919074	0.0
6	0.0	0.0	0.0	3.8911589731215326	0.0
7	0.0	0.0	0.0	4.415279332731263	0.0
8	0.0	0.0	0.0	5.233574220517343	0.0
9	0.0	0.0	0.0	5.458019804439587	0.0
10	0.0	0.0	0.0	7.325442548140472	0.0
11	0.0	0.0	0.0	8.138592043093553	0.0
12	0.0	0.0	0.0	10.819341709086586	0.0
13	0.0	0.0	0.0	11.342574932000971	0.0
14	0.0	0.0	0.0	11.529405920040595	0.0
15	0.0	0.0	0.0	12.214807730864019	0.0
16	0.0	0.0	0.0	12.688538726178162	0.0
17	0.0	0.0	0.0	13.19757776196703	0.0
18	0.0	0.0	0.0	13.683196388998795	0.0
19	0.0	0.0	0.0	14.817489367666706	0.0
20	0.0	0.0	0.0	15.358642751827057	0.0
21	0.0	0.0	0.0	15.941668988007686	0.0
22	0.0	0.0	0.0	16.569406913633696	0.0
23	0.0	0.0	0.0	17.133625851873013	0.0
24	0.0	0.0	0.0	17.5928078053835	0.0
25	0.0	0.0	0.0	18.152591060146094	0.0
26	0.0	0.0	0.0	18.52625303622534	0.0
27	0.0	0.0	0.0	18.965740555099174	0.0
28	0.0	0.0	0.0	19.380210819164283	0.0
29	1.7742733906896778E-4	0.0	0.0	19.781728887477357	0.0
30	1.7742733906896778E-4	0.0	0.0	20.28420311172067	0.0
31	1.7742733906896778E-4	0.0	0.0	20.70381876861878	0.0
32	1.7742733906896778E-4	0.0	0.0	21.188195404277064	0.0
33	1.7742733906896778E-4	0.0	0.0	21.71089634517424	0.0
34	1.7742733906896778E-4	0.0	0.0	22.272986155344732	0.0
35	1.7742733906896778E-4	0.0	0.0	22.78752543864474	0.0
36	1.7742733906896778E-4	0.0	0.0	23.219915863955812	0.0
37	1.7742733906896778E-4	0.0	0.0	23.69134030386206	0.0
38	1.7742733906896778E-4	0.0	0.0	24.208186142569964	0.0
39	1.7742733906896778E-4	0.0	0.0	24.788905823342695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	30	2.1635242E-6	45.000004	41
ACCGCGT	30	2.1635242E-6	45.000004	39
TACGGAG	65	0.0	45.000004	29
ACGATCG	35	1.2104465E-7	45.000004	40
GCCGAAT	30	2.1635242E-6	45.000004	42
GTCGTCT	25	3.888266E-5	45.000004	11
GCGATAG	30	2.1635242E-6	45.000004	9
CCGCGTA	30	2.1635242E-6	45.000004	40
ATACGCC	25	3.888266E-5	45.000004	28
TACCGGT	30	2.1635242E-6	45.000004	40
TATTTCG	20	7.030225E-4	45.0	1
GTCATCG	20	7.030225E-4	45.0	33
TACGGTC	20	7.030225E-4	45.0	9
ATTCCGT	20	7.030225E-4	45.0	37
GCGATAA	20	7.030225E-4	45.0	33
TTACGCG	20	7.030225E-4	45.0	1
TCATACG	20	7.030225E-4	45.0	6
CGATCGA	155	0.0	45.0	41
ATCGAAT	235	0.0	44.042553	43
CATTAGA	1040	0.0	43.269234	17
>>END_MODULE