##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934027.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240234
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.81878085533272	31.0	30.0	31.0	25.0	34.0
2	29.99440129207356	31.0	30.0	33.0	25.0	34.0
3	29.970453807537652	31.0	30.0	33.0	25.0	34.0
4	33.84512600214791	35.0	33.0	37.0	28.0	37.0
5	34.295728331543415	35.0	35.0	37.0	32.0	37.0
6	33.7165263867729	35.0	33.0	37.0	29.0	37.0
7	34.39602637428507	35.0	35.0	37.0	32.0	37.0
8	34.26192795357859	35.0	35.0	37.0	31.0	37.0
9	35.67422180041127	37.0	35.0	39.0	30.0	39.0
10	35.18638910395698	37.0	34.0	39.0	30.0	39.0
11	35.35615275106771	37.0	34.0	39.0	30.0	39.0
12	35.275252462182706	37.0	34.0	39.0	30.0	39.0
13	35.10506422904335	37.0	34.0	39.0	29.0	39.0
14	35.72968855366018	38.0	34.0	40.0	27.0	41.0
15	36.02487158353938	38.0	34.0	40.0	30.0	41.0
16	36.23820941248949	38.0	34.0	40.0	30.0	41.0
17	36.04347844185253	38.0	34.0	40.0	30.0	41.0
18	36.14947925772372	38.0	34.0	40.0	30.0	41.0
19	36.16957632974516	38.0	34.0	40.0	30.0	41.0
20	36.16715369181715	38.0	34.0	40.0	30.0	41.0
21	35.86280459884946	38.0	34.0	40.0	29.0	41.0
22	36.089054838199424	38.0	34.0	40.0	30.0	41.0
23	36.18588959098213	38.0	34.0	40.0	30.0	41.0
24	36.032751400717636	38.0	34.0	40.0	30.0	41.0
25	35.62023277304628	38.0	34.0	40.0	29.0	41.0
26	35.71131480140197	38.0	34.0	40.0	29.0	41.0
27	35.713966382776796	38.0	34.0	40.0	29.0	41.0
28	35.641915798762874	38.0	34.0	40.0	29.0	41.0
29	35.8170783486101	38.0	34.0	40.0	30.0	41.0
30	35.36129773470866	37.0	34.0	40.0	27.0	41.0
31	35.48743724868253	38.0	34.0	40.0	29.0	41.0
32	35.53479524130639	38.0	34.0	40.0	29.0	41.0
33	35.51327455730662	38.0	34.0	40.0	29.0	41.0
34	35.46343565024102	38.0	34.0	40.0	28.0	41.0
35	35.55542512716768	38.0	34.0	40.0	29.0	41.0
36	35.327422429797615	38.0	34.0	40.0	27.0	41.0
37	35.3531889740836	38.0	34.0	40.0	28.0	41.0
38	35.19320745606367	37.0	34.0	40.0	27.0	41.0
39	35.22125927220959	37.0	34.0	40.0	28.0	41.0
40	35.18629336397013	37.0	34.0	40.0	27.0	41.0
41	35.04267505848465	37.0	33.0	40.0	27.0	41.0
42	35.29215265116511	37.0	34.0	40.0	28.0	41.0
43	35.19458944196076	37.0	34.0	40.0	28.0	41.0
44	35.07213383617639	37.0	33.0	40.0	27.0	41.0
45	35.168689694214805	37.0	34.0	40.0	28.0	41.0
46	35.04868586461534	37.0	33.0	40.0	27.0	41.0
47	34.91899980852003	37.0	33.0	40.0	27.0	41.0
48	34.96194127392459	37.0	33.0	40.0	27.0	41.0
49	34.97486617214882	36.0	34.0	40.0	28.0	41.0
50	34.7370189065661	36.0	33.0	39.0	27.0	41.0
51	33.55683625132163	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	4.0
12	2.0
13	7.0
14	6.0
15	7.0
16	19.0
17	49.0
18	96.0
19	162.0
20	325.0
21	553.0
22	864.0
23	1207.0
24	1656.0
25	2235.0
26	2915.0
27	3787.0
28	4924.0
29	6371.0
30	8130.0
31	10265.0
32	13374.0
33	17245.0
34	23832.0
35	24211.0
36	28122.0
37	35040.0
38	38982.0
39	15838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.24594353838341	4.583031544244362	54.15136908181189	17.01965583556033
2	19.16006893279053	6.8932790529234	52.772297010414846	21.174355003871227
3	20.540389786624708	7.045630510252504	51.40030137282816	21.01367833029463
4	20.840513832346797	4.870667765595211	48.034832704779504	26.253985697278488
5	19.199613709966115	6.148172198772863	50.28763622135085	24.36457786991017
6	20.12079888775111	8.565814997044548	53.319679978687454	17.993706136516895
7	78.78651647976557	2.3743516737847266	13.224606009141088	5.614525837308624
8	78.74072779040435	5.5833062763805295	8.473821357509761	7.202144575705353
9	72.26246076741843	6.250572358617015	12.061989560178827	9.424977313785726
10	39.81368166038113	26.291032909579826	19.006052432211927	14.889232997827119
11	30.17557881065961	25.14673193636205	25.825653321345023	18.852035931633324
12	26.445881931783177	22.019780713804042	31.214565798346612	20.31977155606617
13	23.249415153558616	27.459477009915332	31.49762315076134	17.793484685764714
14	18.597700575272444	29.614459235578643	30.26007975557165	21.52776043357726
15	15.362937802309414	26.00214790579185	38.835468751300816	19.79944554059792
16	17.745614692341633	25.330302954619242	32.49706536127276	24.42701699176636
17	18.53859153991525	24.250106146507157	30.108560819867296	27.102741493710298
18	19.406911594528665	23.437565040751934	33.586003646444716	23.56951971827468
19	21.21431604185919	25.422712854966406	30.277146448879012	23.085824654295394
20	24.245527277571036	25.331135476243993	31.003937827285068	19.419399418899904
21	22.636262976930826	28.29699376441303	29.19986346645354	19.866879792202603
22	20.30561868844543	25.302829741002526	30.003663095148898	24.387888475403148
23	20.55787274074444	26.55286096056345	27.4453241422946	25.443942156397515
24	21.52859295520201	24.1772605043416	29.664410533063595	24.62973600739279
25	17.70898374085267	27.631808986238415	28.9039020288552	25.755305244053716
26	19.82691875421464	26.93249082144909	29.303096147922442	23.93749427641383
27	20.393449719856473	28.47931600023311	28.63166745756221	22.49556682234821
28	17.02589974774595	27.862001215481573	32.657325774036984	22.4547732627355
29	19.909338395064815	23.301864015917815	29.586569761149544	27.20222782786783
30	20.44007093084243	26.420073761415953	28.794425435200676	24.34542987254094
31	21.9598391568221	27.525662479082897	26.93748595119758	23.577012412897425
32	21.908222816087648	28.097604835285594	26.072079722270786	23.92209262635597
33	21.006601896484263	27.914866338653148	26.493751925206254	24.584779839656335
34	18.913642531864767	26.030869901845698	30.074427433252577	24.981060133036955
35	20.180740444733054	25.118426201120574	28.16254152201603	26.53829183213034
36	24.106496166237918	27.34334024326282	27.147697661446756	21.402465929052507
37	20.31977155606617	27.735874189332065	29.91083693398936	22.033517320612404
38	17.259005802675727	29.97327605584555	26.944146124195576	25.823572017283148
39	18.814988719331986	25.794850021229305	30.178076375533852	25.21208488390486
40	21.662212675974256	23.251496457620487	30.425751558896742	24.660539307508515
41	18.373752258214907	25.364436341233965	29.644430014069616	26.617381386481515
42	22.257049376857562	24.56646436391185	29.141170691908723	24.03531556732186
43	22.470591173605733	25.49597475794434	27.367067109568172	24.66636695888176
44	19.638352606208947	25.07388629419649	27.93609564008425	27.35166545951031
45	19.867296053014975	24.215972759892438	27.383717542063156	28.53301364502943
46	22.724094008341865	24.493202460933922	29.115778782353875	23.666924748370338
47	17.42759143168744	24.70549547524497	33.74085266864807	24.126060424419524
48	18.336288785101193	24.604760358650317	31.419782378847287	25.639168477401203
49	19.87562126926247	22.17795982250639	33.52606208946277	24.42035681876837
50	20.899206606891614	22.17254843194552	30.430330427832864	26.497914533330004
51	18.359183129781794	22.250805464671945	28.39564757694581	30.994363828600445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	153.0
2	210.0
3	336.5
4	463.0
5	365.5
6	268.0
7	313.5
8	359.0
9	469.0
10	579.0
11	665.5
12	752.0
13	673.0
14	594.0
15	616.5
16	639.0
17	588.0
18	537.0
19	617.5
20	698.0
21	955.5
22	1213.0
23	1208.0
24	1203.0
25	1372.0
26	1861.5
27	2182.0
28	2406.5
29	2631.0
30	3148.5
31	3666.0
32	4259.0
33	4852.0
34	5574.5
35	6297.0
36	6817.5
37	7338.0
38	8029.0
39	8720.0
40	10041.0
41	11362.0
42	13160.5
43	14959.0
44	17508.5
45	20058.0
46	24361.0
47	28664.0
48	29930.0
49	31196.0
50	29860.5
51	28525.0
52	22847.0
53	17169.0
54	14735.5
55	12302.0
56	10635.0
57	8968.0
58	8036.0
59	7104.0
60	6061.5
61	5019.0
62	4394.0
63	3769.0
64	3066.0
65	2363.0
66	1867.5
67	1372.0
68	1145.5
69	919.0
70	819.5
71	720.0
72	554.0
73	388.0
74	337.0
75	190.0
76	94.0
77	84.0
78	74.0
79	48.0
80	22.0
81	24.0
82	26.0
83	31.5
84	37.0
85	18.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.93939242571825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7826739483887	29.468351690435156
2	11.062242047775879	8.83638452508804
3	3.890649101596698	4.661704837783161
4	1.9823237587026306	3.166912260545968
5	1.306957935548422	2.6099552935887513
6	0.9525993246341768	2.282774295062314
7	0.7806311752199108	2.1824554392800355
8	0.6670279734856381	2.1312553593579593
9	0.6378454996456413	2.2927645545593047
>10	4.805728102722308	32.84630818285505
>50	0.08337849668570477	2.311496291116162
>100	0.03960478592570976	2.7947750942830742
>500	0.002084462417142619	0.636046521308391
>1k	0.006253387251427857	3.7788156547366314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2149	0.8945444857930186	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	1623	0.6755912984839781	No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	1523	0.633965217246518	TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1320	0.5494642723344739	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	1275	0.5307325357776168	No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1188	0.49451784510102653	TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	872	0.36297942839065245	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT	656	0.27306709291773856	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA	352	0.1465238059558597	No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	310	0.12904085183612643	TruSeq Adapter, Index 20 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	291	0.12113189640100902	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT	267	0.11114163690401858	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.10697902878027256	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC	253	0.10531398553077416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6876628620428415	0.0
2	0.0	0.0	0.0	2.879276039195118	0.0
3	0.0	0.0	0.0	3.567771422862709	0.0
4	0.0	0.0	0.0	4.739129348884838	0.0
5	0.0	0.0	0.0	6.75757802808928	0.0
6	0.0	0.0	0.0	7.615907823205708	0.0
7	0.0	0.0	0.0	8.60036464447164	0.0
8	0.0	0.0	0.0	10.11472147989044	0.0
9	0.0	0.0	0.0	10.770332259380437	0.0
10	0.0	0.0	0.0	12.506972368607274	0.0
11	0.0	0.0	0.0	14.735216497248516	0.0
12	0.0	0.0	0.0	17.38055395988911	0.0
13	0.0	0.0	0.0	18.147306376283122	0.0
14	0.0	0.0	0.0	18.420789730013237	0.0
15	0.0	0.0	0.0	18.999392259213934	0.0
16	0.0	0.0	0.0	20.049618288835052	0.0
17	0.0	0.0	0.0	21.55814747288061	0.0
18	0.0	0.0	0.0	23.40759426226096	0.0
19	0.0	0.0	0.0	24.67760600081587	0.0
20	0.0	0.0	0.0	25.752807679179465	0.0
21	0.0	0.0	0.0	27.04321619754073	0.0
22	0.0	0.0	0.0	28.219569253311356	0.0
23	0.0	0.0	0.0	29.402998742892347	0.0
24	0.0	0.0	0.0	30.278811492128508	0.0
25	0.0	0.0	0.0	31.127983549372694	0.0
26	0.0	0.0	0.0	31.881415619770724	0.0
27	0.0	0.0	0.0	32.6127858671129	0.0
28	0.0	0.0	0.0	33.34124228876845	0.0
29	0.0	0.0	0.0	34.09259305510461	0.0
30	0.0	0.0	0.0	35.09161900480365	0.0
31	0.0	0.0	0.0	35.868777941507034	0.0
32	0.0	0.0	0.0	36.66841496207864	0.0
33	0.0	0.0	0.0	37.40561286079406	0.0
34	0.0	0.0	0.0	38.075792768717164	0.0
35	0.0	0.0	0.0	38.88750135284764	0.0
36	0.0	0.0	0.0	39.59139838657309	0.0
37	0.0	0.0	0.0	40.302788114921285	0.0
38	0.0	0.0	0.0	41.03082827576446	0.0
39	0.0	0.0	0.0	41.831714078773196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	40	6.7757355E-9	45.000004	1
CGTGGCA	20	7.022134E-4	45.000004	15
AGGTAAG	20	7.022134E-4	45.000004	31
TTGGATA	20	7.022134E-4	45.000004	5
GGCACGT	20	7.022134E-4	45.000004	11
ACACCGC	20	7.022134E-4	45.000004	33
GGCAATC	20	7.022134E-4	45.000004	16
TCGGGTA	20	7.022134E-4	45.000004	5
TATGCCA	20	7.022134E-4	45.000004	10
TCTACGG	20	7.022134E-4	45.000004	2
CTACGGC	20	7.022134E-4	45.000004	6
GAGCGGT	20	7.022134E-4	45.000004	32
GTCACGT	20	7.022134E-4	45.000004	38
CGGGCTA	20	7.022134E-4	45.000004	6
GCTAGTG	20	7.022134E-4	45.000004	1
CGCAGAC	20	7.022134E-4	45.000004	37
GTATCAA	20	7.022134E-4	45.000004	35
TTACGTG	20	7.022134E-4	45.000004	1
CGGCTAT	20	7.022134E-4	45.000004	14
GTCTTAC	20	7.022134E-4	45.000004	31
>>END_MODULE