##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934026.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321109
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.759358971564172	31.0	30.0	31.0	25.0	34.0
2	29.789650866216768	31.0	30.0	33.0	25.0	34.0
3	29.856030195354226	31.0	30.0	33.0	25.0	34.0
4	33.8302757007745	35.0	33.0	37.0	28.0	37.0
5	34.33408593343693	35.0	35.0	37.0	32.0	37.0
6	33.62312485791429	35.0	33.0	37.0	28.0	37.0
7	34.38038173953393	35.0	35.0	37.0	32.0	37.0
8	34.20867369024226	35.0	35.0	37.0	30.0	37.0
9	35.7077970408802	37.0	35.0	39.0	30.0	39.0
10	35.1554736865052	37.0	34.0	39.0	30.0	39.0
11	35.33008729123133	37.0	34.0	39.0	30.0	39.0
12	35.345729954626	37.0	34.0	39.0	30.0	39.0
13	35.20517954962334	37.0	34.0	39.0	29.0	39.0
14	35.887455661473204	38.0	34.0	40.0	29.0	41.0
15	36.091869116094536	38.0	34.0	40.0	30.0	41.0
16	36.35108950543273	38.0	35.0	40.0	31.0	41.0
17	36.09171340572827	38.0	34.0	40.0	30.0	41.0
18	36.22131737198272	38.0	35.0	40.0	30.0	41.0
19	36.21815956575493	38.0	34.0	40.0	30.0	41.0
20	36.18367594804256	38.0	34.0	40.0	30.0	41.0
21	35.94471970576969	38.0	34.0	40.0	29.0	41.0
22	36.13744865450673	38.0	34.0	40.0	30.0	41.0
23	36.17623610674257	38.0	34.0	40.0	30.0	41.0
24	36.054190321666475	38.0	34.0	40.0	30.0	41.0
25	35.6497046174352	38.0	34.0	40.0	29.0	41.0
26	35.78630620754946	38.0	34.0	40.0	29.0	41.0
27	35.760498771445214	38.0	34.0	40.0	29.0	41.0
28	35.68728687143618	38.0	34.0	40.0	29.0	41.0
29	35.86558146922073	38.0	34.0	40.0	29.0	41.0
30	35.401455580503814	38.0	34.0	40.0	27.0	41.0
31	35.58128859670704	38.0	34.0	40.0	29.0	41.0
32	35.56044520707922	38.0	34.0	40.0	29.0	41.0
33	35.53147996474717	38.0	34.0	40.0	28.0	41.0
34	35.6170428110081	38.0	34.0	40.0	29.0	41.0
35	35.642597996319004	38.0	34.0	40.0	29.0	41.0
36	35.40717637936028	38.0	34.0	40.0	27.0	41.0
37	35.53594885225889	38.0	34.0	40.0	29.0	41.0
38	35.404410963255465	38.0	34.0	40.0	28.0	41.0
39	35.39688703835769	38.0	34.0	40.0	28.0	41.0
40	35.29597114998334	38.0	34.0	40.0	27.0	41.0
41	35.206219694869965	37.0	34.0	40.0	27.0	41.0
42	35.455564932779836	38.0	34.0	40.0	28.0	41.0
43	35.421096886104095	38.0	34.0	40.0	28.0	41.0
44	35.43838696517382	38.0	34.0	40.0	28.0	41.0
45	35.50910749932266	38.0	34.0	40.0	29.0	41.0
46	35.42033390530941	38.0	34.0	40.0	28.0	41.0
47	35.34901855756145	37.0	34.0	40.0	28.0	41.0
48	35.366663656266255	37.0	34.0	40.0	28.0	41.0
49	35.44110255396143	37.0	34.0	40.0	29.0	41.0
50	35.23847976855211	37.0	34.0	40.0	28.0	41.0
51	34.23903098324868	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	6.0
12	3.0
13	7.0
14	11.0
15	14.0
16	29.0
17	75.0
18	131.0
19	263.0
20	500.0
21	857.0
22	1264.0
23	1758.0
24	2285.0
25	2868.0
26	3608.0
27	4703.0
28	6108.0
29	7876.0
30	10252.0
31	13537.0
32	17461.0
33	22109.0
34	30265.0
35	31664.0
36	36896.0
37	47093.0
38	54759.0
39	24699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.248663226505645	4.393523694446435	50.8534485174816	16.50436456156632
2	18.74254536621521	9.422968524706564	50.632028376657146	21.202457732421077
3	20.476847425640514	9.421722841776468	48.81426556091545	21.287164171667563
4	20.1358417235269	4.458922048276442	49.86655621611354	25.538680012083127
5	21.742772703349953	5.231556885668106	48.07557558336889	24.950094827613054
6	20.985086061119432	10.575536655777322	49.73357956332585	18.705797719777397
7	77.57677299608544	2.3157245670473268	15.28266102787527	4.824841408991962
8	77.99376535693487	6.675614822381185	8.340781479186195	6.989838341497746
9	72.15493804284526	5.244325135701584	11.056681687526664	11.544055133926486
10	38.99236707784584	25.49196690220455	19.385629178876954	16.130036841072656
11	31.50861545456527	24.859471394448615	25.89681385448555	17.735099296500564
12	26.855055448461425	21.409552519549436	30.500546543385582	21.234845488603558
13	24.39140603346527	24.728986107521123	33.160702440604275	17.718905418409324
14	19.235212965067934	28.2794938790255	30.240821652460692	22.24447150344587
15	15.891177139226867	24.95943744958877	38.712399839306904	20.43698557187746
16	18.230881102678527	23.92894624566736	32.64997243926517	25.19020021238894
17	18.216555748982433	25.493212585134646	31.388095631078546	24.902136034804382
18	19.293448642049896	23.56956672033484	32.293395700525366	24.843588937089898
19	20.821901597276938	25.82113861648225	29.47503807118455	23.881921715056258
20	24.714037912359977	24.83144352852147	32.111214571998914	18.34330398711964
21	23.68510381210119	28.308767427882742	28.774964264470942	19.231164495545126
22	19.514245941409303	24.656425076843068	30.82224416008271	25.007084821664915
23	20.41736606572846	27.573814499126463	28.37323151951518	23.6355879156299
24	21.70041948372671	24.43313641162347	29.818535139158353	24.04790896549147
25	18.05119134001227	28.75005060586904	27.35986845588258	25.838889598236115
26	18.11098412065685	26.070275202501332	30.04306948730805	25.77567118953377
27	19.85462880205787	26.662286015029164	30.992279880040734	22.490805302872232
28	15.45767947955367	26.211660215067155	34.59635201753922	23.734308287839955
29	17.46104905187958	23.953548483536743	33.649632990666724	24.935769473916956
30	18.796109732209313	27.235300162873045	32.143602328181395	21.82498777673625
31	20.139890193049713	25.41380029834106	28.146828646970345	26.299480861638884
32	20.86923754862056	27.33931468753601	28.37883709270061	23.41261067114282
33	20.112796589320137	24.64957382072754	29.575004126324707	25.662625463627613
34	17.98330162032207	24.113618740054	30.644734342544123	27.258345297079806
35	16.73201311704125	23.204893042549415	31.334219844351917	28.728873996057413
36	18.205033181879053	25.293591895586857	31.54878872906085	24.952586193473245
37	16.660697769293293	23.87382477601064	36.03605006399696	23.42942739069911
38	17.622676412059455	26.61744142954573	30.881414099262244	24.87846805913257
39	18.406522395821977	25.277398017495617	32.5472035975323	23.7688759891501
40	19.403380160630814	25.295148999249477	28.25022033016826	27.05125050995145
41	16.549209147049755	23.573615189857648	29.092301990912745	30.784873672179852
42	18.510848341217468	21.84959001460563	31.328925691899013	28.310635952277885
43	19.728191984653186	22.843956413554274	31.977615077746187	25.450236524046353
44	18.161745700058233	23.223266865768323	32.72658193946604	25.888405494707406
45	18.388148572603072	22.462466016212563	29.707669358379864	29.4417160528045
46	20.13739882718952	24.374277893176462	31.170101118311848	24.31822216132217
47	14.789059166824972	24.00244153854299	35.41974843433226	25.788750860299775
48	17.022880081218528	22.674543535061304	33.27748521530072	27.025091168419447
49	18.2383552002591	20.549719877051096	35.93110127713642	25.28082364555338
50	18.369774749384167	21.385261702412574	32.15232210869207	28.092641439511194
51	16.67502312298939	21.18657527506236	30.052100688551242	32.08630091339701
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	147.5
2	249.0
3	411.5
4	574.0
5	425.5
6	277.0
7	322.5
8	368.0
9	399.5
10	431.0
11	467.0
12	503.0
13	579.5
14	656.0
15	645.0
16	634.0
17	600.0
18	566.0
19	688.5
20	811.0
21	921.5
22	1032.0
23	1400.0
24	1768.0
25	1953.0
26	2558.0
27	2978.0
28	3539.5
29	4101.0
30	4908.5
31	5716.0
32	6219.0
33	6722.0
34	7902.5
35	9083.0
36	9544.0
37	10005.0
38	11248.0
39	12491.0
40	14895.0
41	17299.0
42	20275.5
43	23252.0
44	32915.0
45	42578.0
46	41135.0
47	39692.0
48	37757.5
49	35823.0
50	30256.0
51	24689.0
52	21468.5
53	18248.0
54	15981.5
55	13715.0
56	12845.5
57	11976.0
58	10970.5
59	9965.0
60	9137.0
61	8309.0
62	6987.0
63	5665.0
64	4550.5
65	3436.0
66	2764.5
67	2093.0
68	1711.5
69	1330.0
70	1033.0
71	736.0
72	588.5
73	441.0
74	352.5
75	270.5
76	277.0
77	192.5
78	108.0
79	71.5
80	35.0
81	29.0
82	23.0
83	13.0
84	3.0
85	2.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	321109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.18733215806787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01442746407137	27.882261939016466
2	11.288301361704828	8.62140227199584
3	3.927538774442273	4.499466832299536
4	2.048926832721834	3.1297219811490655
5	1.2991498270269635	2.4805532983887537
6	0.949139707657462	2.174706796843616
7	0.8130972571202599	2.1735010524125693
8	0.72245830394918	2.2071004178609352
9	0.6425471107960928	2.208344395245951
>10	5.14942865629986	33.30378262575263
>50	0.09555810050195485	2.502470818319453
>100	0.03954128296632615	2.54839925822827
>500	0.0032951069138605123	0.8776736119561627
>1k	0.006590213827721025	5.390614700530754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC	3577	1.113951960237801	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	2984	0.9292794658511596	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	2377	0.7402470812091844	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2025	0.6306269833607903	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1954	0.6085161113515971	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1443	0.4493801170319113	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1434	0.4465773304391967	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTACCTT	1342	0.4179266230470028	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTA	876	0.27280456169089	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTC	690	0.21488030544145445	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT	638	0.1986864273502144	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	586	0.18249254925897437	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1142073252384705E-4	0.0	0.0	0.4898648122600114	0.0
2	3.1142073252384705E-4	0.0	0.0	2.3892198599229544	0.0
3	3.1142073252384705E-4	0.0	0.0	2.9921303980891225	0.0
4	3.1142073252384705E-4	0.0	0.0	4.117916346162829	0.0
5	3.1142073252384705E-4	0.0	0.0	6.3719796081704345	0.0
6	3.1142073252384705E-4	0.0	0.0	7.188524768847961	0.0
7	3.1142073252384705E-4	0.0	0.0	8.206247722735894	0.0
8	3.1142073252384705E-4	0.0	0.0	9.6767764217135	0.0
9	3.1142073252384705E-4	0.0	0.0	10.242005051244282	0.0
10	3.1142073252384705E-4	0.0	0.0	12.345652099442868	0.0
11	3.1142073252384705E-4	0.0	0.0	14.282689055741196	0.0
12	3.1142073252384705E-4	0.0	0.0	17.320909722243847	0.0
13	3.1142073252384705E-4	0.0	0.0	18.0567969131977	0.0
14	3.1142073252384705E-4	0.0	0.0	18.316210383390064	0.0
15	3.1142073252384705E-4	0.0	0.0	18.987322061978954	0.0
16	3.1142073252384705E-4	0.0	0.0	19.893244972890827	0.0
17	3.1142073252384705E-4	0.0	0.0	21.186886695794886	0.0
18	3.1142073252384705E-4	0.0	0.0	22.548106717656623	0.0
19	3.1142073252384705E-4	0.0	0.0	23.91524373343631	0.0
20	3.1142073252384705E-4	0.0	0.0	24.896841882351477	0.0
21	3.1142073252384705E-4	0.0	0.0	26.152178855155103	0.0
22	3.1142073252384705E-4	0.0	0.0	27.309729717946244	0.0
23	3.1142073252384705E-4	0.0	0.0	28.447972495320904	0.0
24	3.1142073252384705E-4	0.0	0.0	29.2993967780411	0.0
25	3.1142073252384705E-4	0.0	0.0	30.05770626173667	0.0
26	3.1142073252384705E-4	0.0	0.0	30.784562251447326	0.0
27	3.1142073252384705E-4	0.0	0.0	31.4332516372945	0.0
28	3.1142073252384705E-4	0.0	0.0	32.13986527939111	0.0
29	3.1142073252384705E-4	0.0	0.0	32.89910902528425	0.0
30	3.1142073252384705E-4	0.0	0.0	33.726242490867584	0.0
31	3.1142073252384705E-4	0.0	0.0	34.583272346773214	0.0
32	3.1142073252384705E-4	0.0	0.0	35.34625314145664	0.0
33	3.1142073252384705E-4	0.0	0.0	36.06034088113382	0.0
34	3.1142073252384705E-4	0.0	0.0	36.804013590400764	0.0
35	3.1142073252384705E-4	0.0	0.0	37.59097378148853	0.0
36	3.1142073252384705E-4	0.0	0.0	38.365477143275335	0.0
37	3.1142073252384705E-4	0.0	0.0	39.137800559934476	0.0
38	3.1142073252384705E-4	0.0	0.0	39.84379136056604	0.0
39	3.1142073252384705E-4	0.0	0.0	40.65379668586056	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCTA	25	3.8845057E-5	45.000004	31
ACACGGC	25	3.8845057E-5	45.000004	18
TTCACGG	25	3.8845057E-5	45.000004	2
TACGGGA	60	0.0	45.000004	4
CGGGATC	60	0.0	45.000004	6
TAGGGCG	25	3.8845057E-5	45.000004	5
GAACGAG	25	3.8845057E-5	45.000004	12
AGTTCGA	25	3.8845057E-5	45.000004	33
CGAGCGA	25	3.8845057E-5	45.000004	15
CACCGGA	25	3.8845057E-5	45.000004	41
CTCTATC	25	3.8845057E-5	45.000004	28
CCTTGCG	50	2.1827873E-11	45.000004	1
CTAGCGG	25	3.8845057E-5	45.000004	2
CCGGGCA	20	7.025687E-4	45.0	5
GGTAATC	35	1.2082819E-7	45.0	8
CGCGGGT	20	7.025687E-4	45.0	4
TTCGTAG	20	7.025687E-4	45.0	1
CAATTCG	20	7.025687E-4	45.0	26
AGGAACG	35	1.2082819E-7	45.0	10
AGTATGT	20	7.025687E-4	45.0	37
>>END_MODULE