##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934024.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	575055
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.064953787029065	31.0	30.0	31.0	26.0	34.0
2	30.333095095251757	31.0	30.0	33.0	27.0	34.0
3	30.350973385154465	31.0	30.0	34.0	26.0	34.0
4	34.05838398066272	35.0	33.0	37.0	30.0	37.0
5	34.466849257897074	35.0	35.0	37.0	32.0	37.0
6	33.96453382719914	35.0	35.0	37.0	30.0	37.0
7	34.385924824582	35.0	35.0	37.0	32.0	37.0
8	34.552089800106074	35.0	35.0	37.0	32.0	37.0
9	35.95806314178644	37.0	35.0	39.0	31.0	39.0
10	35.34992131187452	37.0	34.0	39.0	30.0	39.0
11	35.433880237542496	37.0	35.0	39.0	30.0	39.0
12	35.32200224326369	37.0	34.0	39.0	30.0	39.0
13	35.259841232577756	37.0	34.0	39.0	30.0	39.0
14	35.8601281616541	38.0	34.0	40.0	27.0	41.0
15	36.15789272330473	38.0	34.0	40.0	30.0	41.0
16	36.335693107615796	38.0	35.0	40.0	30.0	41.0
17	36.108711340654374	38.0	34.0	40.0	30.0	41.0
18	36.25111163280034	38.0	35.0	40.0	30.0	41.0
19	36.244924398535794	38.0	34.0	40.0	30.0	41.0
20	36.21119197294172	38.0	34.0	40.0	30.0	41.0
21	35.93353331420473	38.0	34.0	40.0	29.0	41.0
22	36.17274347671092	38.0	34.0	40.0	30.0	41.0
23	36.277761257618835	38.0	34.0	40.0	30.0	41.0
24	36.222792602446724	38.0	34.0	40.0	30.0	41.0
25	35.769185556164196	38.0	34.0	40.0	29.0	41.0
26	35.87413030057994	38.0	34.0	40.0	30.0	41.0
27	35.916420168505624	38.0	34.0	40.0	30.0	41.0
28	35.81536722574362	38.0	34.0	40.0	29.0	41.0
29	35.937858117919156	38.0	34.0	40.0	30.0	41.0
30	35.50965038126788	38.0	34.0	40.0	29.0	41.0
31	35.62119275547556	38.0	34.0	40.0	29.0	41.0
32	35.62089017572232	38.0	34.0	40.0	29.0	41.0
33	35.657759692551146	38.0	34.0	40.0	29.0	41.0
34	35.60368138699776	38.0	34.0	40.0	29.0	41.0
35	35.580803575310185	38.0	34.0	40.0	29.0	41.0
36	35.361398474928485	38.0	34.0	40.0	28.0	41.0
37	35.43651476815261	38.0	34.0	40.0	29.0	41.0
38	35.379175904913446	38.0	34.0	40.0	28.0	41.0
39	35.27892810252932	37.0	34.0	40.0	28.0	41.0
40	35.21810261627149	37.0	34.0	40.0	27.0	41.0
41	35.07609706897601	37.0	33.0	40.0	27.0	41.0
42	35.266362347949325	37.0	34.0	40.0	28.0	41.0
43	35.22277695176983	37.0	34.0	40.0	28.0	41.0
44	35.231976071854	37.0	34.0	40.0	28.0	41.0
45	35.28954273939014	37.0	34.0	40.0	28.0	41.0
46	35.215934128039926	37.0	34.0	40.0	28.0	41.0
47	35.138529358061405	37.0	34.0	40.0	28.0	41.0
48	35.090956517202706	37.0	34.0	40.0	28.0	41.0
49	35.08992705045604	37.0	34.0	40.0	28.0	41.0
50	34.90812704871708	36.0	34.0	40.0	27.0	41.0
51	33.81216231490901	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	11.0
12	9.0
13	11.0
14	19.0
15	37.0
16	77.0
17	153.0
18	243.0
19	443.0
20	784.0
21	1437.0
22	2307.0
23	3090.0
24	4137.0
25	5224.0
26	6769.0
27	8728.0
28	10985.0
29	14058.0
30	17891.0
31	23664.0
32	30318.0
33	40413.0
34	55061.0
35	54406.0
36	65393.0
37	83416.0
38	98535.0
39	47427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.696498595786487	4.765283320725844	54.02283259862101	15.515385484866664
2	19.116606237664225	5.672153098399284	54.39792715479389	20.813313509142603
3	21.604368277816903	5.880307101059898	51.335611376303135	21.17971324482006
4	20.054951265531123	5.089252332385598	48.142177704741286	26.713618697341996
5	20.020867569189036	5.574597212440549	49.67490066167584	24.729634556694577
6	22.18848631869995	7.868116962725305	51.88129831059638	18.062098407978368
7	84.49748284946658	2.395596942901114	9.244333150742102	3.8625870568902108
8	86.7177922111798	4.10795489127127	5.245411308483536	3.928841589065394
9	81.82921633582875	4.897966281486118	6.774830233629826	6.497987149055308
10	44.90127031327438	26.879168079575	14.220900609506918	13.998660997643702
11	34.27672135708758	24.188816721878776	22.95467390075732	18.57978802027632
12	29.558390067037067	21.971637495543906	28.17400074775456	20.295971689664466
13	27.227656485031865	22.63018319986784	29.330237977236962	20.811922337863333
14	20.94703984836233	25.39304936049595	31.311961464555566	22.347949326586154
15	19.283720687586406	23.879976697881073	34.25933171609672	22.576970898435803
16	24.876055333837634	24.31019641599499	29.00035648764031	21.81339176252706
17	25.831268313465667	24.425489735764405	27.06002034587996	22.683221604889965
18	26.381128761596717	23.127526932206486	28.756901513768245	21.734442792428553
19	26.372955630331013	25.262279260244675	25.963255688586308	22.401509420838007
20	26.905600333881107	28.179043743641913	26.521984853622698	18.393371068854282
21	26.47277216961856	27.088365460695062	27.81194842232482	18.626913947361558
22	24.916399300936433	22.79468920364139	30.443348897062023	21.845562598360157
23	24.08413108311379	25.25532340384833	29.20937997235047	21.451165540687413
24	24.222030936171322	25.3960055994644	28.39606646320787	21.98589700115641
25	24.130909217379205	26.85221413603916	25.646938118962538	23.369938527619098
26	21.87025588856718	26.388954100042604	27.216701011207622	24.52408900018259
27	22.12310126857431	25.04977784733634	29.379972350470823	23.447148533618524
28	18.684647555451217	26.995678674213767	29.0222674352888	25.29740633504621
29	20.82652963629566	26.831868256079854	28.894801366825785	23.446800740798707
30	22.983192911982332	25.606420255453827	29.07130622288303	22.339080609680813
31	24.86770830616202	25.02595403917886	26.906817608750465	23.199520045908653
32	25.471302744954833	26.455208632217786	25.953169696811607	22.120318926015774
33	26.059942092495504	24.311413690864352	25.34818408673953	24.280460129900618
34	21.819825929693682	25.004043091530377	29.08330507516672	24.09282590360922
35	21.471337524236812	27.359122170922785	28.01592891114763	23.153611393692778
36	23.74051177713436	27.78812461416734	25.982732086496075	22.488631522202223
37	21.809392145099167	28.707862726174017	26.31939553607916	23.16334959264766
38	22.86163932145621	27.896983766770134	24.63260035996557	24.608776551808088
39	22.233351592456373	27.046804218726905	25.106989766196275	25.612854422620444
40	23.783985879611517	23.8361548025841	26.256792828512054	26.12306648929233
41	21.13693472798254	24.629470224587212	25.663284381493945	28.5703106659363
42	20.932780342749822	24.6312091886863	27.771778351635927	26.664232116927945
43	21.97215918477363	24.301501595499563	27.86724748067576	25.85909173905105
44	21.44403578788116	24.06030727495631	28.02201528549443	26.4736416516681
45	21.20753667040544	23.908321812696176	26.34826234012399	28.535879176774397
46	21.67427463460017	24.98334941875125	27.486066550156075	25.85630939649251
47	18.650389962699222	25.746059072610443	30.20719757240612	25.396353392284215
48	20.38222430897914	26.41086504769109	28.422498717513978	24.784411925815792
49	22.29543261079375	24.128300771230577	28.805940301362476	24.770326316613193
50	21.13954317413117	24.77589100173027	27.146968550834266	26.93759727330429
51	19.92087713349158	24.430880524471572	26.66892731999548	28.97931502204137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	243.5
2	381.0
3	750.0
4	1119.0
5	835.0
6	551.0
7	580.5
8	610.0
9	697.0
10	784.0
11	876.5
12	969.0
13	966.5
14	964.0
15	1034.0
16	1104.0
17	1069.5
18	1035.0
19	1142.0
20	1249.0
21	1398.5
22	1548.0
23	2122.5
24	2697.0
25	2924.0
26	4207.0
27	5263.0
28	6175.0
29	7087.0
30	7778.5
31	8470.0
32	10236.5
33	12003.0
34	14118.5
35	16234.0
36	16947.0
37	17660.0
38	19425.0
39	21190.0
40	23599.0
41	26008.0
42	27785.0
43	29562.0
44	31951.5
45	34341.0
46	36094.0
47	37847.0
48	43645.0
49	49443.0
50	50012.5
51	50582.0
52	49709.5
53	48837.0
54	45240.0
55	41643.0
56	39037.5
57	36432.0
58	34271.5
59	32111.0
60	29264.0
61	26417.0
62	22854.0
63	19291.0
64	17022.5
65	14754.0
66	11858.0
67	8962.0
68	7381.5
69	5801.0
70	4869.5
71	3938.0
72	3083.5
73	2229.0
74	1844.0
75	1059.5
76	660.0
77	484.5
78	309.0
79	254.5
80	200.0
81	118.5
82	37.0
83	25.0
84	13.0
85	7.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	575055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.47704514788205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41710196025484	24.577876369501105
2	9.17976445632575	6.146227783026801
3	3.1596977761233367	3.1733203531483034
4	1.6972724516131334	2.2727866596363726
5	1.171933014413893	1.96164272169137
6	0.9673893344839193	1.943120185565864
7	0.8555444302158346	2.004876966144816
8	0.7406201000786535	1.9835018022209605
9	0.7372540849836753	2.221297945961307
>10	7.929851292193042	47.531724838466936
>50	0.09886947498542573	2.1989428135186486
>100	0.039442609701622854	2.1276400241287208
>500	0.0026295073134415233	0.6132039887497173
>1k	0.0026295073134415233	1.2438375482390907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGC	2141	0.37231221361434996	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG	1634	0.2841467337906809	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	1105	0.19215553294902227	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1097	0.19076436166975333	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	1088	0.18919929398057575	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	908	0.15789794019702463	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCACCCGT	759	0.13198737512064063	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	743	0.12920503256210275	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20919738112006678	0.0
2	0.0	0.0	0.0	0.7413203954404362	0.0
3	0.0	0.0	0.0	0.9341715140290928	0.0
4	0.0	0.0	0.0	1.3050925563641738	0.0
5	0.0	0.0	0.0	2.0657154533044664	0.0
6	0.0	0.0	0.0	2.3829025049777846	0.0
7	0.0	0.0	0.0	2.7360861135021866	0.0
8	0.0	0.0	0.0	3.25638417194877	0.0
9	0.0	0.0	0.0	3.4669727243481057	0.0
10	0.0	0.0	0.0	4.226378346419038	0.0
11	0.0	0.0	0.0	5.216544504438706	0.0
12	0.0	0.0	0.0	6.527027849510047	0.0
13	0.0	0.0	0.0	6.845953865282452	0.0
14	0.0	0.0	0.0	6.969768109137386	0.0
15	0.0	0.0	0.0	7.210440740450913	0.0
16	0.0	0.0	0.0	7.596838563267862	0.0
17	0.0	0.0	0.0	8.135047951935032	0.0
18	0.0	0.0	0.0	8.73412108407022	0.0
19	0.0	0.0	0.0	9.330585770056777	0.0
20	0.0	0.0	0.0	9.830364052134144	0.0
21	0.0	0.0	0.0	10.526123588178523	0.0
22	0.0	0.0	0.0	11.259096955943345	0.0
23	0.0	0.0	0.0	11.932771647929329	0.0
24	0.0	0.0	0.0	12.486457817078366	0.0
25	0.0	0.0	0.0	12.958064880750538	0.0
26	0.0	0.0	0.0	13.414369060350749	0.0
27	0.0	0.0	0.0	13.879020267626576	0.0
28	0.0	0.0	0.0	14.349757849249203	0.0
29	0.0	0.0	0.0	14.90848701428559	0.0
30	0.0	0.0	0.0	15.592943283685909	0.0
31	0.0	0.0	0.0	16.130283190303537	0.0
32	0.0	0.0	0.0	16.615454173948578	0.0
33	0.0	0.0	0.0	17.140099642642877	0.0
34	0.0	0.0	0.0	17.729782368643	0.0
35	0.0	0.0	0.0	18.360504647381553	0.0
36	0.0	0.0	0.0	18.952795819530305	0.0
37	0.0	0.0	0.0	19.53082748606655	0.0
38	0.0	0.0	0.0	20.205545556511986	0.0
39	0.0	0.0	0.0	21.345436523462972	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	25	3.8883656E-5	45.0	43
GCGTTAG	20	7.0303446E-4	45.0	1
AAACACG	110	0.0	42.954544	40
CGAATAT	80	0.0	42.1875	14
GCGAGAC	110	0.0	40.909092	21
AACACGT	105	0.0	40.714287	41
CCGATGA	300	0.0	40.5	18
TGTTGCG	90	0.0	40.000004	1
CTACGAA	85	0.0	39.705883	11
TACGAAT	85	0.0	39.705883	12
ACGGGAT	40	3.4542063E-7	39.375	5
CGTCGAA	40	3.4542063E-7	39.375	42
CAATCGG	40	3.4542063E-7	39.375	20
ACATACG	75	0.0	39.0	17
TTGGGAT	1590	0.0	38.915096	5
TTTGGGA	2455	0.0	38.67617	4
CGGGTAT	105	0.0	38.57143	6
TTGGGAC	960	0.0	38.437504	5
GTTACCG	30	1.1392124E-4	37.500004	38
GCGATTC	120	0.0	37.500004	9
>>END_MODULE