##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791454
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.084745291577274	31.0	30.0	31.0	26.0	34.0
2	30.322953702931567	31.0	30.0	33.0	26.0	34.0
3	30.356379524267993	31.0	30.0	34.0	26.0	34.0
4	34.069564371397455	35.0	33.0	37.0	30.0	37.0
5	34.4738064372661	35.0	35.0	37.0	32.0	37.0
6	33.96308818958524	35.0	35.0	37.0	30.0	37.0
7	34.473851923169256	35.0	35.0	37.0	32.0	37.0
8	34.64468307696973	35.0	35.0	37.0	32.0	37.0
9	36.004154379155324	37.0	35.0	39.0	31.0	39.0
10	35.32843601775972	37.0	34.0	39.0	30.0	39.0
11	35.44767604939769	37.0	35.0	39.0	30.0	39.0
12	35.485391444101616	37.0	35.0	39.0	30.0	39.0
13	35.50317137824813	37.0	35.0	39.0	30.0	39.0
14	36.14377462240383	38.0	35.0	40.0	29.0	41.0
15	36.414448597138936	38.0	35.0	40.0	30.0	41.0
16	36.5828942680181	38.0	35.0	40.0	31.0	41.0
17	36.324760504084885	38.0	35.0	40.0	30.0	41.0
18	36.45716238720128	38.0	35.0	40.0	30.0	41.0
19	36.465905788586575	38.0	35.0	40.0	30.0	41.0
20	36.44752316622318	38.0	35.0	40.0	30.0	41.0
21	36.15299183528038	38.0	34.0	40.0	30.0	41.0
22	36.36351828406957	38.0	35.0	40.0	30.0	41.0
23	36.433697978657	38.0	35.0	40.0	30.0	41.0
24	36.346180573981556	38.0	35.0	40.0	30.0	41.0
25	35.891034728487064	38.0	34.0	40.0	29.0	41.0
26	36.018348507936025	38.0	34.0	40.0	30.0	41.0
27	36.02394959151132	38.0	34.0	40.0	30.0	41.0
28	35.928692002314726	38.0	34.0	40.0	29.0	41.0
29	36.06095869121895	38.0	34.0	40.0	30.0	41.0
30	35.57089357056759	38.0	34.0	40.0	28.0	41.0
31	35.74236531750424	38.0	34.0	40.0	29.0	41.0
32	35.77171762351318	38.0	34.0	40.0	29.0	41.0
33	35.737455366957526	38.0	34.0	40.0	29.0	41.0
34	35.69321148165275	38.0	34.0	40.0	29.0	41.0
35	35.7061496941073	38.0	34.0	40.0	29.0	41.0
36	35.49527957404979	38.0	34.0	40.0	28.0	41.0
37	35.53824859056875	38.0	34.0	40.0	28.0	41.0
38	35.4034928119638	38.0	34.0	40.0	27.0	41.0
39	35.33064334756031	38.0	34.0	40.0	27.0	41.0
40	35.27528447641935	38.0	34.0	40.0	27.0	41.0
41	35.16514794290003	38.0	34.0	40.0	27.0	41.0
42	35.373570668668044	38.0	34.0	40.0	28.0	41.0
43	35.29186787861329	38.0	34.0	40.0	27.0	41.0
44	35.28876851971182	38.0	34.0	40.0	28.0	41.0
45	35.337169311166534	38.0	34.0	40.0	28.0	41.0
46	35.22661076954567	37.0	34.0	40.0	27.0	41.0
47	35.168392603992146	37.0	34.0	40.0	27.0	41.0
48	35.101048197368385	37.0	34.0	40.0	27.0	41.0
49	35.127488142077745	37.0	34.0	40.0	27.0	41.0
50	34.893547824636684	37.0	33.0	40.0	27.0	41.0
51	33.78281492038703	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	14.0
12	9.0
13	12.0
14	19.0
15	48.0
16	94.0
17	147.0
18	358.0
19	607.0
20	1227.0
21	2022.0
22	3194.0
23	4292.0
24	5873.0
25	7437.0
26	9689.0
27	11943.0
28	15141.0
29	18955.0
30	24224.0
31	30902.0
32	39145.0
33	51253.0
34	69061.0
35	73872.0
36	90399.0
37	116557.0
38	142145.0
39	72804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.854760984213865	4.208330490464386	55.557998317021585	15.378910208300168
2	19.584713704144523	5.697235720585151	54.53418644671706	20.18386412855327
3	20.908227136384426	5.764453777477907	52.864853800726266	20.462465285411408
4	19.279579103776086	4.747212093185453	50.089076560356006	25.884132242682455
5	19.71674917304101	5.513649561440084	50.826706289942315	23.942894975576596
6	21.7409982134148	7.8604694650605085	52.680509543195186	17.718022778329505
7	84.62551203228489	2.0803483209384246	9.810172164143463	3.4839674826332296
8	86.91345296125864	3.774571863936502	5.652507915810647	3.6594672589942054
9	81.59021244443771	4.9886664291291725	7.28507279008003	6.136048336353092
10	40.695732158786235	31.240855438218773	14.705592491793585	13.35781991120141
11	29.347757418624454	24.781351790502036	27.28686695626025	18.584023834613255
12	25.598202801426233	22.860077780894407	30.46734238502806	21.0743770326513
13	24.895066548403317	23.28511827598319	32.38457320324365	19.43524197236984
14	20.547498654375364	26.655876399639144	31.63241325459218	21.16421169139331
15	18.932875441908184	25.085601942753467	35.25170129912794	20.72982131621042
16	23.012834605675124	25.02242707725275	30.540246179815885	21.42449213725624
17	23.735934116196265	25.344618891306382	28.96820282669618	21.95124416580117
18	23.134509396629493	24.710974990334243	30.465699838525044	21.688815774511216
19	24.160974611285052	25.564467423248853	28.549985217081474	21.724572748384617
20	25.13121419564498	28.33417987653104	28.25129445299411	18.28331147482987
21	25.278790681454637	27.06853462108979	29.34699932023845	18.305675377217124
22	23.126296664114403	24.047007153921772	30.785870056882647	22.040826125081182
23	22.03551943637912	25.98773902210362	30.269226006817828	21.707515534699425
24	22.003300254973755	26.96732848655765	29.075473748316387	21.953897510152203
25	21.98409509586154	28.31623821472884	27.49306971725457	22.206596972155047
26	21.30736593661792	27.808817695027127	27.888544375289026	22.995271993065927
27	22.012776484798863	27.094562665676087	29.696482676188385	21.19617817333667
28	19.579912414366472	27.43520154045592	30.42362537810157	22.561260667076038
29	21.640929226461676	26.07264604133658	29.938316061325104	22.34810867087664
30	22.975687784760705	27.453648601182127	28.780826175621073	20.78983743843609
31	23.294215456615294	27.444172371357023	27.38276640209033	21.878845769937357
32	24.804221091813293	27.45023715844509	26.847927990761306	20.897613758980306
33	23.89538747672006	27.007634050747104	26.893792943114825	22.203185529418008
34	22.158457724643505	26.475828032962117	28.141749236215873	23.223965006178503
35	22.70593110907267	26.69125432431954	28.331905581373018	22.27090898523477
36	24.263949642051212	27.832824143917396	27.151167345164723	20.75205886886667
37	22.348993118993647	27.811218339916156	28.336959570613075	21.502828970477122
38	21.623998362507486	29.05803748543819	27.624852486689054	21.693111665365265
39	20.75938715326475	27.89284026614307	28.58195169902483	22.765820881567343
40	22.665751894614218	26.604452059121563	27.972061547480966	22.757734498783254
41	21.383428474680777	25.834603148129897	27.46628357428227	25.315684802907057
42	22.691653589469507	25.731754467094742	28.69895154993215	22.8776403935036
43	21.76449926338107	26.00504893525082	29.35710729871856	22.873344502649555
44	21.97234457087841	25.970050059763423	28.807612318593375	23.249993050764793
45	21.864694600065196	26.233615598632394	27.004728006934076	24.896961794368337
46	22.721472125985844	26.95519891238152	27.763205442135614	22.560123519497026
47	20.541307517556294	26.948755076100444	30.024233878406072	22.48570352793719
48	20.43252039916407	27.55119059351523	29.381240097339834	22.63504890998087
49	22.118910258840057	25.5241618590594	30.466836986104056	21.890090895996483
50	21.47793807346984	26.133799311141264	28.43879745380022	23.94946516158867
51	20.89167532162324	25.365719296383617	27.651385930199357	26.091219451793783
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	317.0
2	506.0
3	1664.0
4	2822.0
5	1980.0
6	1138.0
7	1217.0
8	1296.0
9	1402.5
10	1509.0
11	1728.0
12	1947.0
13	1982.5
14	2018.0
15	2068.5
16	2119.0
17	2070.0
18	2021.0
19	2249.5
20	2478.0
21	2763.0
22	3048.0
23	3327.0
24	3606.0
25	4613.5
26	7122.5
27	8624.0
28	10021.5
29	11419.0
30	13902.5
31	16386.0
32	17783.0
33	19180.0
34	22763.5
35	26347.0
36	28129.0
37	29911.0
38	33383.0
39	36855.0
40	39047.5
41	41240.0
42	44471.5
43	47703.0
44	51364.5
45	55026.0
46	65118.0
47	75210.0
48	74544.5
49	73879.0
50	71778.5
51	69678.0
52	62785.5
53	55893.0
54	50636.0
55	45379.0
56	41967.5
57	38556.0
58	34904.0
59	31252.0
60	28167.0
61	25082.0
62	21840.0
63	18598.0
64	15868.0
65	13138.0
66	10746.5
67	8355.0
68	6939.5
69	5524.0
70	4375.5
71	3227.0
72	2578.5
73	1930.0
74	1675.5
75	1100.5
76	780.0
77	576.0
78	372.0
79	267.0
80	162.0
81	107.5
82	53.0
83	31.5
84	10.0
85	8.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	791454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.21527518810667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14025439836858	25.367292067564172
2	9.119766280156865	6.240706258535662
3	3.250932307819369	3.336946305898393
4	1.7255471945811414	2.3616028845063686
5	1.1966624860481316	2.0472068133710346
6	0.9711798969124333	1.993751245800959
7	0.8036240209596648	1.9247351917435416
8	0.7206499612462179	1.9725789390670208
9	0.642566266496119	1.9787023471283103
>10	7.3008717561988234	46.421406120923
>50	0.08754161207355735	1.99645620563321
>100	0.035540396464954625	2.0909220331237037
>500	0.001496437745892818	0.3014460598718277
>1k	0.003366984928258841	1.9662475268327582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	3102	0.39193686556641316	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	2452	0.3098095404154884	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	1884	0.23804289320668034	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.21441549350941433	No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1481	0.18712395161310702	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	1315	0.16614989626687085	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1237	0.15629461724875987	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT	1124	0.1420170976455991	No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1069	0.13506786244052085	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1825753612970558	0.0
2	0.0	0.0	0.0	0.7280271500301976	0.0
3	0.0	0.0	0.0	0.9544458679847471	0.0
4	0.0	0.0	0.0	1.4348275452521562	0.0
5	0.0	0.0	0.0	2.368931106545674	0.0
6	0.0	0.0	0.0	2.856767417942167	0.0
7	0.0	0.0	0.0	3.3356328984375594	0.0
8	0.0	0.0	0.0	4.084634103813993	0.0
9	0.0	0.0	0.0	4.420471688815774	0.0
10	0.0	0.0	0.0	5.342066626740151	0.0
11	0.0	0.0	0.0	6.45824015040672	0.0
12	0.0	0.0	0.0	7.947398079989488	0.0
13	0.0	0.0	0.0	8.314696748010624	0.0
14	0.0	0.0	0.0	8.461515135434277	0.0
15	0.0	0.0	0.0	8.727607668923273	0.0
16	0.0	0.0	0.0	9.12333502641973	0.0
17	0.0	0.0	0.0	9.722611800559477	0.0
18	0.0	0.0	0.0	10.351075362560554	0.0
19	0.0	0.0	0.0	11.011126357311985	0.0
20	0.0	0.0	0.0	11.536867588008905	0.0
21	0.0	0.0	0.0	12.231538409054727	0.0
22	0.0	0.0	0.0	13.019202632117596	0.0
23	0.0	0.0	0.0	13.735605606895662	0.0
24	0.0	0.0	0.0	14.306706391022093	0.0
25	0.0	0.0	0.0	14.821707894583893	0.0
26	0.0	0.0	0.0	15.273281833182978	0.0
27	0.0	0.0	0.0	15.728519913981103	0.0
28	0.0	0.0	0.0	16.220525766500643	0.0
29	0.0	0.0	0.0	16.76193436384174	0.0
30	0.0	0.0	0.0	17.374225160274634	0.0
31	0.0	0.0	0.0	17.93509161618995	0.0
32	0.0	0.0	0.0	18.454894409529803	0.0
33	0.0	0.0	0.0	18.975834350448668	0.0
34	0.0	0.0	0.0	19.547440533499103	0.0
35	0.0	0.0	0.0	20.1776729917342	0.0
36	0.0	0.0	0.0	20.745488682854596	0.0
37	0.0	0.0	0.0	21.314062472360995	0.0
38	0.0	0.0	0.0	21.934566001308983	0.0
39	0.0	0.0	0.0	22.789321931533607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATTCG	20	7.031953E-4	45.000004	15
CGTAAAC	20	7.031953E-4	45.000004	20
CGATCGA	80	0.0	45.000004	41
TTGTTCG	265	0.0	41.603775	1
TCGTACG	60	3.6379788E-12	41.250004	30
CCGATCG	55	6.002665E-11	40.90909	40
ACCAACG	55	6.002665E-11	40.90909	36
CGTTAGG	50	1.0804797E-9	40.5	2
CGTTTGG	500	0.0	39.600002	2
CGTTTTT	1020	0.0	39.485294	1
TTTCGCG	275	0.0	39.272728	1
GCGATTT	440	0.0	38.863632	9
CCGATGA	635	0.0	38.622047	18
TTGTGCG	210	0.0	38.57143	1
GTTTACG	35	6.245824E-6	38.571426	1
ACGATCG	35	6.245824E-6	38.571426	40
TTGGGAT	3030	0.0	38.09406	5
TTTGGGA	4690	0.0	38.091686	4
TTTGGGC	1655	0.0	37.794563	4
TGGGCGA	1030	0.0	37.79126	6
>>END_MODULE