##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108073
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01814514263507	31.0	30.0	31.0	26.0	34.0
2	30.355472689756	31.0	30.0	33.0	27.0	34.0
3	30.342240892729915	31.0	30.0	34.0	26.0	34.0
4	34.053232537266474	35.0	33.0	37.0	30.0	37.0
5	34.445411897513715	35.0	35.0	37.0	32.0	37.0
6	34.01878359997409	35.0	35.0	37.0	30.0	37.0
7	34.4869301305599	35.0	35.0	37.0	32.0	37.0
8	34.666382907849325	35.0	35.0	37.0	32.0	37.0
9	35.88850129079419	37.0	35.0	39.0	30.0	39.0
10	35.29454165240162	37.0	34.0	39.0	30.0	39.0
11	35.39025473522526	37.0	35.0	39.0	30.0	39.0
12	35.2159743876824	37.0	34.0	39.0	30.0	39.0
13	34.95308726508934	37.0	34.0	39.0	27.0	39.0
14	35.4842282531252	38.0	33.0	40.0	27.0	41.0
15	36.00608847723298	38.0	34.0	40.0	30.0	41.0
16	36.22409852599632	38.0	34.0	40.0	31.0	41.0
17	36.079557336244946	38.0	34.0	40.0	30.0	41.0
18	36.18027629472671	38.0	34.0	40.0	30.0	41.0
19	36.18009123462845	38.0	34.0	40.0	30.0	41.0
20	36.237395094056794	38.0	34.0	40.0	30.0	41.0
21	35.81766953818253	38.0	34.0	40.0	29.0	41.0
22	36.05485181312631	38.0	34.0	40.0	30.0	41.0
23	36.195238403671596	38.0	34.0	40.0	30.0	41.0
24	36.07789179536054	38.0	34.0	40.0	30.0	41.0
25	35.63668076207748	37.0	34.0	40.0	29.0	41.0
26	35.67270271020514	37.0	34.0	40.0	29.0	41.0
27	35.71853284354094	38.0	34.0	40.0	29.0	41.0
28	35.60688608625651	38.0	34.0	40.0	29.0	41.0
29	35.790289896643934	38.0	34.0	40.0	30.0	41.0
30	35.38534138961628	37.0	34.0	40.0	29.0	41.0
31	35.481933507906696	37.0	34.0	40.0	29.0	41.0
32	35.539885077678974	37.0	34.0	40.0	29.0	41.0
33	35.50247517881432	38.0	34.0	40.0	29.0	41.0
34	35.51030322097101	37.0	34.0	40.0	29.0	41.0
35	35.57480591822194	38.0	34.0	40.0	29.0	41.0
36	35.31258501198264	37.0	34.0	40.0	28.0	41.0
37	35.31478722715202	37.0	34.0	40.0	28.0	41.0
38	35.241161067056524	37.0	34.0	40.0	28.0	41.0
39	35.219360987480684	37.0	34.0	40.0	28.0	41.0
40	35.16140016470349	37.0	34.0	40.0	28.0	41.0
41	35.10863953068759	37.0	34.0	40.0	27.0	41.0
42	35.290220499107086	37.0	34.0	40.0	29.0	41.0
43	35.20038307440341	37.0	34.0	40.0	28.0	41.0
44	35.069786163056456	37.0	33.0	40.0	28.0	41.0
45	35.216298242854364	37.0	34.0	40.0	28.0	41.0
46	35.194516669288355	37.0	34.0	40.0	28.0	41.0
47	35.14430986462854	37.0	34.0	40.0	28.0	41.0
48	35.17817586261138	37.0	34.0	40.0	28.0	41.0
49	35.206841671832926	37.0	34.0	40.0	28.0	41.0
50	34.94995049642371	37.0	34.0	40.0	27.0	41.0
51	33.87954438203807	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	0.0
13	4.0
14	6.0
15	3.0
16	13.0
17	17.0
18	39.0
19	86.0
20	111.0
21	215.0
22	312.0
23	473.0
24	672.0
25	983.0
26	1259.0
27	1637.0
28	2048.0
29	2802.0
30	3461.0
31	4549.0
32	5974.0
33	8073.0
34	11394.0
35	11021.0
36	12256.0
37	15484.0
38	17555.0
39	7621.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.56110221794528	4.508063993781981	56.52383111415432	18.40700267411842
2	19.63672702710205	3.9871198171606226	56.615435862796446	19.760717292940882
3	21.00987295624254	3.8973656695011702	53.20570355222859	21.887057822027703
4	20.731357508350836	4.648709668464834	48.12395325381918	26.49597956936515
5	17.477075680327186	5.199263460808898	51.83255762308809	25.49110323577582
6	20.950653724797128	6.045913410380021	53.55639243844439	19.447040426378468
7	82.4933147039501	2.596393178684778	9.92662367103717	4.983668446327945
8	83.2909237274805	2.6685666170088735	7.765121723279635	6.275387932230991
9	76.82399859354325	6.243002415034282	10.765871216677617	6.1671277747448485
10	40.21355935340002	28.346580552034272	17.712102005126166	13.727758089439545
11	32.897208368417644	26.71435048532011	23.889408085275694	16.499033060986555
12	31.379715562628963	21.240272778584846	29.815957732273557	17.564053926512635
13	24.401099256983706	29.314444865970223	28.10230122232195	18.182154654724123
14	17.45764437000916	32.46046653650773	29.729904786579443	20.351984306903667
15	15.379419466471736	25.79737769840756	40.717848121177354	18.105354713943353
16	17.290164981077606	22.851220933998313	37.54221683491714	22.31639725000694
17	17.444690163130478	23.531316795129218	29.711398776752752	29.312594264987556
18	19.74221128311419	22.821611318275608	33.96778103689173	23.46839636171847
19	23.875528577905676	25.713175353696112	29.12290766426397	21.288388404134242
20	27.196432041305414	24.220665661173467	29.756738500828146	18.826163796692978
21	23.094574963219305	27.200133243270752	29.2468979301028	20.458393863407142
22	19.773671499819567	25.745560870892824	27.71367501596143	26.767092613326177
23	20.57220582384129	27.07614297743192	27.441636671509073	24.910014527217715
24	21.445689487661117	23.200059219231445	31.78037067537683	23.57388061773061
25	18.462520703598493	24.443663079585097	32.18472699009003	24.90908922672638
26	18.781749373108916	29.144189575564667	30.020449140858496	22.053611910467925
27	20.432485449649775	29.964005810887084	29.60776512172328	19.995743617739862
28	17.95082953189048	27.638725676163332	33.59858614084924	20.811858651096944
29	17.667687581542104	26.405300121214363	32.570577294976545	23.356435002266988
30	21.82968919156496	25.379141876324336	30.125008096379297	22.666160835731404
31	23.21116282512746	28.041231389893866	26.667160160262043	22.080445624716628
32	24.923894034587732	29.180276294726713	25.109879433345977	20.785950237339577
33	25.128385443172668	27.343554819427606	25.801078900372897	21.726980837026826
34	20.382519223117708	26.767092613326177	29.450464038196404	23.39992412535971
35	21.54377133974258	24.859123000194312	28.201308374894747	25.39579728516836
36	25.87325233869699	24.153118725306044	28.53071534980985	21.442913586187114
37	23.03443043128256	27.93667243437306	29.53096518094251	19.497931953401867
38	22.307144245093593	28.742609162325465	26.30166646618489	22.64858012639605
39	21.682566413442768	26.267430348005515	29.041481221026526	23.00852201752519
40	23.356435002266988	23.17877730793075	29.51430977209849	23.950477917703775
41	20.536119104679244	23.581283021661285	29.85944685536628	26.023151018293188
42	25.409676792538377	23.097350864693308	28.15134214836268	23.34163019440563
43	27.26120307569883	23.423056637643075	27.159420021652032	22.15632026500606
44	21.434585881765102	27.35558372581496	27.580431745209253	23.62939864721068
45	21.44939068962646	26.36366159910431	25.871401737714322	26.31554597355491
46	22.817910116310273	25.558650171643244	28.748160965273474	22.875278746773013
47	20.873853784016358	25.171874566265394	31.438934794074374	22.51533685564387
48	24.029128459467213	22.740184875038167	29.654030146290005	23.57665651920461
49	23.110305071571993	20.822036956501623	32.52801347237516	23.53964449955123
50	21.944426452490447	24.15496932628871	28.69726943825007	25.20333478297077
51	20.29554097693226	23.814458745477594	26.0795943482646	29.81040592932555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	59.5
2	91.0
3	160.5
4	230.0
5	181.0
6	132.0
7	170.5
8	209.0
9	230.5
10	252.0
11	273.0
12	294.0
13	295.0
14	296.0
15	300.5
16	305.0
17	322.5
18	340.0
19	372.0
20	404.0
21	449.0
22	494.0
23	467.5
24	441.0
25	604.5
26	810.5
27	853.0
28	1037.5
29	1222.0
30	1476.5
31	1731.0
32	2147.5
33	2564.0
34	2642.0
35	2720.0
36	2873.5
37	3027.0
38	3542.0
39	4057.0
40	4853.0
41	5649.0
42	6430.0
43	7211.0
44	8048.5
45	8886.0
46	8868.5
47	8851.0
48	9664.0
49	10477.0
50	11679.0
51	12881.0
52	12106.0
53	11331.0
54	8979.0
55	6627.0
56	5459.5
57	4292.0
58	3841.5
59	3391.0
60	3075.0
61	2759.0
62	2509.5
63	2260.0
64	1727.0
65	1194.0
66	991.0
67	788.0
68	611.0
69	434.0
70	345.5
71	257.0
72	219.0
73	181.0
74	120.0
75	56.5
76	54.0
77	37.5
78	21.0
79	16.5
80	12.0
81	6.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	108073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.23954179119669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5916211750434	32.99899142246445
2	10.28842734935475	9.10310623374941
3	3.7334504611909396	4.954984131096573
4	1.9409759260421242	3.4347154238338904
5	1.3427872246972454	2.9702145771839406
6	1.068791700654661	2.8369713064317637
7	1.0436928740248061	3.232074616231621
8	0.8575432431867144	3.0349856115773597
9	0.7696973499822217	3.0645952273000656
>10	4.287716215933571	29.723427683140102
>50	0.05228922214553137	1.572085534777419
>100	0.01882411997239129	1.6313047662228308
>500	0.004183137771642509	1.4425434659905805
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	922	0.8531270530104652	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	637	0.5894164129801153	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	469	0.4339659304359091	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	383	0.3543900881811368	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	161	0.1489733791048643	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	149	0.13786977320884958	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC	136	0.12584086682150028	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT	126	0.11658786190815466	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.1091854579774782	No Hit
TTTGTCGGGAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC	111	0.10270835453813625	No Hit
TTTTTTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA	110	0.10178305404680171	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6347561370555088	0.0
2	0.0	0.0	0.0	2.4835065187419616	0.0
3	0.0	0.0	0.0	3.116412054814801	0.0
4	0.0	0.0	0.0	4.068546260398064	0.0
5	0.0	0.0	0.0	5.463899401330582	0.0
6	0.0	0.0	0.0	6.231898809138268	0.0
7	0.0	0.0	0.0	7.043387340038677	0.0
8	0.0	0.0	0.0	8.297169505797008	0.0
9	0.0	0.0	0.0	8.937477445800523	0.0
10	0.0	0.0	0.0	10.106131966356074	0.0
11	0.0	0.0	0.0	12.146419549748781	0.0
12	0.0	0.0	0.0	14.053463862389311	0.0
13	0.0	0.0	0.0	14.685444097970816	0.0
14	0.0	0.0	0.0	14.899188511469099	0.0
15	0.0	0.0	0.0	15.24617619571956	0.0
16	0.0	0.0	0.0	16.123361061504724	0.0
17	0.0	0.0	0.0	17.64825627122408	0.0
18	0.0	0.0	0.0	19.692245056582124	0.0
19	0.0	0.0	0.0	20.64067806020005	0.0
20	0.0	0.0	0.0	21.710325428182802	0.0
21	0.0	0.0	0.0	22.975211199837148	0.0
22	0.0	0.0	0.0	24.066140479120595	0.0
23	0.0	0.0	0.0	25.178351669704735	0.0
24	0.0	0.0	0.0	26.06201363892924	0.0
25	0.0	0.0	0.0	26.778196219222192	0.0
26	0.0	0.0	0.0	27.407400553329694	0.0
27	0.0	0.0	0.0	27.956103744691088	0.0
28	0.0	0.0	0.0	28.62602130041731	0.0
29	0.0	0.0	0.0	29.366261693484958	0.0
30	0.0	0.0	0.0	30.22216464796943	0.0
31	0.0	0.0	0.0	31.08917120834991	0.0
32	0.0	0.0	0.0	31.78129597586816	0.0
33	0.0	0.0	0.0	32.48267374829976	0.0
34	0.0	0.0	0.0	33.177574417292014	0.0
35	0.0	0.0	0.0	33.90023410102431	0.0
36	0.0	0.0	0.0	34.58218056313788	0.0
37	0.0	0.0	0.0	35.3511052714369	0.0
38	0.0	0.0	0.0	36.12002997973592	0.0
39	0.0	0.0	0.0	36.782545131531464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	30	2.147226E-6	45.000004	1
CTCGTTG	20	7.004915E-4	45.000004	1
ACGCCGG	30	2.147226E-6	45.000004	27
CATGCAC	65	0.0	45.000004	45
CTTTTCG	40	6.717528E-9	45.000004	1
TATGGGA	30	2.147226E-6	45.000004	4
TGGGTGA	30	2.147226E-6	45.000004	6
TTATGGG	40	6.717528E-9	45.000004	3
CACGCCG	30	2.147226E-6	45.000004	26
GAATCCA	40	6.717528E-9	45.000004	9
TTCGTTG	30	2.147226E-6	45.000004	1
GCTTGCG	20	7.004915E-4	45.000004	1
TATTGGG	40	6.717528E-9	45.000004	3
GGCCACC	20	7.004915E-4	45.000004	8
TTCCCCG	20	7.004915E-4	45.000004	1
AGTGCCC	20	7.004915E-4	45.000004	13
ACCTACA	20	7.004915E-4	45.000004	25
CACTCAG	20	7.004915E-4	45.000004	21
CTACGGG	20	7.004915E-4	45.000004	3
TCTCGTG	20	7.004915E-4	45.000004	1
>>END_MODULE