SRR2934019.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526447
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1026	0.19489141357059686	Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	945	0.1795052493413392	Illumina PCR Primer Index 2 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	895	0.17000761710105672	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	877	0.16658846949455502	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	831	0.1578506478334951	Illumina PCR Primer Index 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	700	0.13296685136395497	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.8879138E-5	45.000004	21
TATAACG	25	3.8879138E-5	45.000004	23
GCATCGC	25	3.8879138E-5	45.000004	22
TCGTTTA	20	7.0298003E-4	45.0	38
CGAGTAA	20	7.0298003E-4	45.0	33
TTATGCG	20	7.0298003E-4	45.0	1
TACGGGA	35	1.2102464E-7	45.0	4
GCCGATA	20	7.0298003E-4	45.0	37
TCGAGTA	20	7.0298003E-4	45.0	32
CTTACCG	20	7.0298003E-4	45.0	1
TTTGTCG	220	0.0	41.93182	1
CTTCGTG	50	1.0786607E-9	40.500004	1
CGCTTGG	190	0.0	40.26316	2
TCGTTTG	235	0.0	40.212765	1
TTACACG	270	0.0	40.000004	34
ACACGCG	260	0.0	39.807693	36
TTACGGG	85	0.0	39.705883	3
TCGGCGT	145	0.0	38.793102	4
GCGATCT	180	0.0	38.750004	9
ACACGAC	105	0.0	38.57143	26