##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526447
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.998128966448665	31.0	30.0	31.0	26.0	34.0
2	30.369138773703714	31.0	30.0	33.0	27.0	34.0
3	30.420507667438507	31.0	30.0	34.0	26.0	34.0
4	33.98468411824932	35.0	33.0	37.0	28.0	37.0
5	34.356320769232234	35.0	35.0	37.0	32.0	37.0
6	33.8939057492967	35.0	35.0	37.0	30.0	37.0
7	34.290775709615595	35.0	35.0	37.0	31.0	37.0
8	34.51352747759984	35.0	35.0	37.0	32.0	37.0
9	35.78887523340431	37.0	35.0	39.0	30.0	39.0
10	35.23341380993718	37.0	34.0	39.0	30.0	39.0
11	35.360682081956966	37.0	35.0	39.0	30.0	39.0
12	35.30933028396021	37.0	34.0	39.0	30.0	39.0
13	35.2291968612225	37.0	34.0	39.0	30.0	39.0
14	35.77189536648513	38.0	34.0	40.0	27.0	41.0
15	36.11118308205764	38.0	34.0	40.0	29.0	41.0
16	36.290581957917894	38.0	35.0	40.0	30.0	41.0
17	36.11030550083864	38.0	34.0	40.0	30.0	41.0
18	36.23327514450647	38.0	35.0	40.0	30.0	41.0
19	36.23313837860221	38.0	34.0	40.0	30.0	41.0
20	36.263589687091006	38.0	34.0	40.0	30.0	41.0
21	35.91687672263305	38.0	34.0	40.0	29.0	41.0
22	36.12191730601561	38.0	34.0	40.0	30.0	41.0
23	36.268359398002076	38.0	34.0	40.0	30.0	41.0
24	36.182101901995836	38.0	34.0	40.0	30.0	41.0
25	35.69319798574215	38.0	34.0	40.0	29.0	41.0
26	35.80114427473231	38.0	34.0	40.0	29.0	41.0
27	35.86073431893429	38.0	34.0	40.0	30.0	41.0
28	35.736573672183525	38.0	34.0	40.0	29.0	41.0
29	35.85926788451639	38.0	34.0	40.0	29.0	41.0
30	35.38771044378636	38.0	34.0	40.0	27.0	41.0
31	35.51902090808761	38.0	34.0	40.0	29.0	41.0
32	35.59019996314919	38.0	34.0	40.0	29.0	41.0
33	35.61824837068119	38.0	34.0	40.0	29.0	41.0
34	35.554050075316226	38.0	34.0	40.0	29.0	41.0
35	35.56841239478998	38.0	34.0	40.0	29.0	41.0
36	35.34888792224098	38.0	34.0	40.0	27.0	41.0
37	35.42358869933725	38.0	34.0	40.0	28.0	41.0
38	35.298573265684865	37.0	34.0	40.0	28.0	41.0
39	35.23909719306977	37.0	34.0	40.0	27.0	41.0
40	35.08388498747262	37.0	33.0	40.0	27.0	41.0
41	34.94226579313777	37.0	33.0	40.0	26.0	41.0
42	35.22155126726907	37.0	34.0	40.0	28.0	41.0
43	35.228442749222616	37.0	34.0	40.0	28.0	41.0
44	35.17576128271222	37.0	34.0	40.0	28.0	41.0
45	35.248351685924696	37.0	34.0	40.0	28.0	41.0
46	35.153943321929845	37.0	34.0	40.0	28.0	41.0
47	35.09276907267019	37.0	34.0	40.0	28.0	41.0
48	35.05791656140124	37.0	34.0	40.0	28.0	41.0
49	35.05924622991488	37.0	34.0	40.0	28.0	41.0
50	34.86142004798204	36.0	33.0	40.0	27.0	41.0
51	33.74322581380462	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	18.0
12	15.0
13	21.0
14	22.0
15	45.0
16	75.0
17	177.0
18	296.0
19	536.0
20	855.0
21	1299.0
22	1958.0
23	2754.0
24	3877.0
25	5079.0
26	6526.0
27	8568.0
28	10544.0
29	13257.0
30	16832.0
31	21626.0
32	27571.0
33	36729.0
34	49365.0
35	49612.0
36	60391.0
37	76579.0
38	89198.0
39	42614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.005366162215758	4.9765693412632235	52.61460317942736	17.40346131709365
2	20.959374827855413	4.211060182696453	52.74035183028871	22.08921315915942
3	23.844945455098042	4.179907948948327	50.699310661852	21.27583593410163
4	20.935060889320294	5.242503043991133	45.29572777506567	28.5267082916229
5	19.154064891622518	6.243743434761714	48.91204622687564	25.690145446740125
6	23.238426660233603	6.966323295602406	51.35065828089057	18.444591763273415
7	84.81879467448765	2.7997120317904747	8.348988597142732	4.032504696579143
8	86.10306450601864	3.263006532471455	6.129771847878324	4.504157113631572
9	80.10853894124195	5.972491057979246	8.179550837976093	5.739419162802713
10	43.197700813187275	26.320028416915665	16.371638550509356	14.110632219387705
11	33.51182550190238	25.129975097208266	22.9189263116705	18.439273089218858
12	29.874992164453403	21.95301711283377	28.314531187374985	19.85745953533784
13	27.104152934673387	23.290283732265546	29.17824586330628	20.42731746975479
14	21.083033999623893	25.23349928862735	32.0672356381554	21.616231073593354
15	20.17657997861133	24.045345495368004	34.55618514304384	21.221889382976826
16	25.066720866487984	23.47529760830625	30.369248946237704	21.088732578968063
17	25.0469657914282	22.912088016457496	27.71048177689302	24.330464415221282
18	25.30834063068077	23.1341426582353	29.769758399231073	21.787758311852855
19	26.219543467813473	24.76868516678792	27.093895491853882	21.917875873544727
20	26.87070113420724	27.041468561887523	27.259534198124406	18.82829610578083
21	26.918759153343068	25.0705199193841	28.160099687147994	19.850621240124834
22	24.534473555742554	23.147629296016504	29.580375612359838	22.737521535881104
23	23.256092256200528	25.066530913843177	29.30551413532606	22.37186269463023
24	23.753958138236136	25.145741166727138	28.668412964647917	22.431887730388812
25	24.15513812406567	25.311949730932078	26.571145813348735	23.961766331653518
26	22.333682213024293	26.293814951932482	26.155719379158775	25.21678345588445
27	22.765444574667534	24.85378395166085	28.832722002404797	23.548049471266815
28	20.000873782166106	26.56829652367665	29.281960007370163	24.148869686787084
29	22.018360822646915	25.999768257773336	27.768797238848354	24.21307368073139
30	23.205754805327032	24.129114611727296	29.31767110459362	23.347459478352047
31	24.175083151770263	25.980583040647964	26.945732428905472	22.898601378676293
32	25.592509787310025	26.15381985271072	25.596308840206138	22.65736151977312
33	24.86518111034919	25.068430440291234	26.42108322395227	23.645305225407306
34	21.829357941065293	25.503232044251366	29.49717635393496	23.170233660748377
35	22.158735827158292	26.264182339342803	27.30455297494335	24.27252885855556
36	23.4399664163724	26.316039411374742	26.127036529793124	24.116957642459735
37	22.127013735475746	27.826542842869273	26.43646938818153	23.609974033473456
38	23.1172368728476	27.090856249536994	26.342062923713115	23.449843953902292
39	22.968883857254387	24.95255932695979	27.039379082794657	25.039177732991163
40	23.7796017452849	24.558027683698455	28.02960222016651	23.632768350850135
41	19.610331144445688	26.013824753488958	27.32373819206872	27.052105909996637
42	21.021299390062058	25.169675199972648	27.801089188465316	26.00793622149998
43	21.67530634612791	24.022361225346522	28.210247185376687	26.092085243148883
44	22.86137066029439	24.270059474173088	27.848387397021924	25.020182468510598
45	21.835816330988685	24.370544423275277	26.318888701046827	27.474750544689208
46	21.306608262560147	25.34386177525943	27.4711414444379	25.87838851774253
47	19.710816093547876	26.658334077314528	29.385484198789243	24.245365630348353
48	20.354565606794225	26.30274272623835	28.86843309962826	24.474258567339163
49	22.262829876511788	24.23301870843599	29.38529424614444	24.11885716890779
50	20.873706185048068	25.58738106590027	27.489756803628858	26.049155945422807
51	20.25104141537515	25.625181642216592	26.064922014941676	28.05885492746658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	249.0
2	429.0
3	696.5
4	964.0
5	771.5
6	579.0
7	604.0
8	629.0
9	653.5
10	678.0
11	680.0
12	682.0
13	781.5
14	881.0
15	901.5
16	922.0
17	898.5
18	875.0
19	1023.0
20	1171.0
21	1339.0
22	1507.0
23	1800.0
24	2093.0
25	2527.5
26	3685.5
27	4409.0
28	4953.5
29	5498.0
30	7093.0
31	8688.0
32	9439.5
33	10191.0
34	12219.5
35	14248.0
36	15777.0
37	17306.0
38	18680.0
39	20054.0
40	21908.5
41	23763.0
42	26417.0
43	29071.0
44	31110.0
45	33149.0
46	36618.5
47	40088.0
48	43543.0
49	46998.0
50	46296.5
51	45595.0
52	41755.5
53	37916.0
54	36333.0
55	34750.0
56	32655.0
57	30560.0
58	29386.5
59	28213.0
60	25564.5
61	22916.0
62	20300.0
63	17684.0
64	15833.0
65	13982.0
66	11939.0
67	9896.0
68	8280.0
69	6664.0
70	5455.5
71	4247.0
72	3432.0
73	2617.0
74	2112.0
75	1372.5
76	1138.0
77	809.0
78	480.0
79	306.5
80	133.0
81	117.0
82	101.0
83	57.5
84	14.0
85	20.0
86	26.0
87	15.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.512238224355926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.46642300405712	24.955365074276205
2	8.805574134338864	5.901889961843816
3	2.9519201993951207	2.9677635882425264
4	1.633865882037868	2.1901801066200184
5	1.1182571834423813	1.8737650563809187
6	0.8808831369220104	1.7712219319409015
7	0.7716722366527898	1.81023246780809
8	0.6662708784420043	1.786258272023947
9	0.6683538545275305	2.0158230231983816
>10	7.916756345882633	49.84499642855284
>50	0.08326964556816124	1.9083528004587205
>100	0.03330785822726447	1.9591600956706094
>500	0.0028713670885572823	0.8175355684095038
>1k	5.742734177114564E-4	0.19745562457348184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1026	0.19489141357059686	Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	945	0.1795052493413392	Illumina PCR Primer Index 2 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	895	0.17000761710105672	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	877	0.16658846949455502	Illumina PCR Primer Index 2 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	831	0.1578506478334951	Illumina PCR Primer Index 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	700	0.13296685136395497	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8995264480564994E-4	0.0	0.0	0.18957273951603865	0.0
2	1.8995264480564994E-4	0.0	0.0	0.7985609187629524	0.0
3	1.8995264480564994E-4	0.0	0.0	1.0329624824531245	0.0
4	1.8995264480564994E-4	0.0	0.0	1.4565568803697237	0.0
5	1.8995264480564994E-4	0.0	0.0	2.158621855571406	0.0
6	1.8995264480564994E-4	0.0	0.0	2.549734351226239	0.0
7	1.8995264480564994E-4	0.0	0.0	2.9558531058207187	0.0
8	1.8995264480564994E-4	0.0	0.0	3.569400148542968	0.0
9	1.8995264480564994E-4	0.0	0.0	3.8395128094566027	0.0
10	1.8995264480564994E-4	0.0	0.0	4.3681510199507265	0.0
11	1.8995264480564994E-4	0.0	0.0	5.359513873191413	0.0
12	1.8995264480564994E-4	0.0	0.0	6.341379094191818	0.0
13	1.8995264480564994E-4	0.0	0.0	6.665628258875063	0.0
14	1.8995264480564994E-4	0.0	0.0	6.795745820566933	0.0
15	1.8995264480564994E-4	0.0	0.0	6.996525766126505	0.0
16	1.8995264480564994E-4	0.0	0.0	7.425438838097662	0.0
17	1.8995264480564994E-4	0.0	0.0	7.995866630449029	0.0
18	1.8995264480564994E-4	0.0	0.0	8.635627138154458	0.0
19	1.8995264480564994E-4	0.0	0.0	9.089613959239962	0.0
20	1.8995264480564994E-4	0.0	0.0	9.588429604499598	0.0
21	1.8995264480564994E-4	0.0	0.0	10.222301580216053	0.0
22	1.8995264480564994E-4	0.0	0.0	10.930634992696321	0.0
23	1.8995264480564994E-4	0.0	0.0	11.562037584030302	0.0
24	1.8995264480564994E-4	0.0	0.0	12.059713513421103	0.0
25	1.8995264480564994E-4	0.0	0.0	12.583223002505475	0.0
26	1.8995264480564994E-4	0.0	0.0	13.024672949033805	0.0
27	1.8995264480564994E-4	0.0	0.0	13.488157402359592	0.0
28	1.8995264480564994E-4	0.0	0.0	13.958480150898382	0.0
29	3.799052896112999E-4	0.0	0.0	14.457675701447629	0.0
30	3.799052896112999E-4	0.0	0.0	15.106174030814119	0.0
31	3.799052896112999E-4	0.0	0.0	15.618096408565345	0.0
32	3.799052896112999E-4	0.0	0.0	16.12394030168279	0.0
33	3.799052896112999E-4	0.0	0.0	16.629784194800237	0.0
34	3.799052896112999E-4	0.0	0.0	17.175138238037256	0.0
35	3.799052896112999E-4	0.0	0.0	17.775578548267916	0.0
36	3.799052896112999E-4	0.0	0.0	18.313714391002325	0.0
37	3.799052896112999E-4	0.0	0.0	18.83304492190097	0.0
38	3.799052896112999E-4	0.0	0.0	19.429306273945905	0.0
39	3.799052896112999E-4	0.0	0.0	20.37887954532935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.8879138E-5	45.000004	21
TATAACG	25	3.8879138E-5	45.000004	23
GCATCGC	25	3.8879138E-5	45.000004	22
TCGTTTA	20	7.0298003E-4	45.0	38
CGAGTAA	20	7.0298003E-4	45.0	33
TTATGCG	20	7.0298003E-4	45.0	1
TACGGGA	35	1.2102464E-7	45.0	4
GCCGATA	20	7.0298003E-4	45.0	37
TCGAGTA	20	7.0298003E-4	45.0	32
CTTACCG	20	7.0298003E-4	45.0	1
TTTGTCG	220	0.0	41.93182	1
CTTCGTG	50	1.0786607E-9	40.500004	1
CGCTTGG	190	0.0	40.26316	2
TCGTTTG	235	0.0	40.212765	1
TTACACG	270	0.0	40.000004	34
ACACGCG	260	0.0	39.807693	36
TTACGGG	85	0.0	39.705883	3
TCGGCGT	145	0.0	38.793102	4
GCGATCT	180	0.0	38.750004	9
ACACGAC	105	0.0	38.57143	26
>>END_MODULE