##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934017.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458910
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.066485803316553	31.0	30.0	31.0	26.0	34.0
2	30.4100738706936	31.0	30.0	33.0	27.0	34.0
3	30.388219912401123	31.0	30.0	34.0	26.0	34.0
4	34.07821795123227	35.0	33.0	37.0	30.0	37.0
5	34.4774596326077	35.0	35.0	37.0	32.0	37.0
6	33.971135952583296	35.0	35.0	37.0	30.0	37.0
7	34.40397463554945	35.0	35.0	37.0	32.0	37.0
8	34.57690178902181	35.0	35.0	37.0	32.0	37.0
9	35.93310235122355	37.0	35.0	39.0	31.0	39.0
10	35.39474624654071	37.0	34.0	39.0	30.0	39.0
11	35.51823887036674	37.0	35.0	39.0	30.0	39.0
12	35.36777799568543	37.0	35.0	39.0	30.0	39.0
13	35.248970386350265	37.0	34.0	39.0	30.0	39.0
14	35.763496110348434	38.0	34.0	40.0	27.0	41.0
15	36.1171645856486	38.0	34.0	40.0	29.0	41.0
16	36.32778322546905	38.0	35.0	40.0	30.0	41.0
17	36.148273081867906	38.0	34.0	40.0	30.0	41.0
18	36.306465319997386	38.0	35.0	40.0	30.0	41.0
19	36.27915277505393	38.0	35.0	40.0	30.0	41.0
20	36.29864243533591	38.0	34.0	40.0	30.0	41.0
21	35.9590268244318	38.0	34.0	40.0	29.0	41.0
22	36.202468893682855	38.0	34.0	40.0	30.0	41.0
23	36.30948769911311	38.0	34.0	40.0	30.0	41.0
24	36.247595389074114	38.0	34.0	40.0	30.0	41.0
25	35.76997014665185	38.0	34.0	40.0	29.0	41.0
26	35.86976967161317	38.0	34.0	40.0	30.0	41.0
27	35.909812381512715	38.0	34.0	40.0	30.0	41.0
28	35.80160815846244	38.0	34.0	40.0	29.0	41.0
29	35.91461070798196	38.0	34.0	40.0	30.0	41.0
30	35.4557756422828	38.0	34.0	40.0	28.0	41.0
31	35.60192630363252	38.0	34.0	40.0	29.0	41.0
32	35.60486369876446	38.0	34.0	40.0	29.0	41.0
33	35.6569567017498	38.0	34.0	40.0	29.0	41.0
34	35.58657253056155	38.0	34.0	40.0	29.0	41.0
35	35.63199538035781	38.0	34.0	40.0	29.0	41.0
36	35.395999215532456	38.0	34.0	40.0	28.0	41.0
37	35.45216709158659	38.0	34.0	40.0	29.0	41.0
38	35.35893312414199	38.0	34.0	40.0	28.0	41.0
39	35.31267132989039	37.0	34.0	40.0	28.0	41.0
40	35.1813885075505	37.0	34.0	40.0	27.0	41.0
41	35.04638818068902	37.0	33.0	40.0	27.0	41.0
42	35.291067965396266	37.0	34.0	40.0	28.0	41.0
43	35.27360920441917	37.0	34.0	40.0	28.0	41.0
44	35.20140768342377	37.0	34.0	40.0	28.0	41.0
45	35.239393345100346	37.0	34.0	40.0	28.0	41.0
46	35.10462617942516	37.0	34.0	40.0	28.0	41.0
47	35.044485846898084	37.0	33.0	40.0	28.0	41.0
48	35.026456167876056	36.0	34.0	40.0	28.0	41.0
49	35.04605914013641	36.0	34.0	40.0	28.0	41.0
50	34.84761935891569	36.0	33.0	40.0	27.0	41.0
51	33.70859209866858	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	16.0
11	7.0
12	15.0
13	18.0
14	25.0
15	42.0
16	72.0
17	124.0
18	206.0
19	374.0
20	754.0
21	1217.0
22	1788.0
23	2406.0
24	3113.0
25	4276.0
26	5600.0
27	7054.0
28	8720.0
29	11110.0
30	14398.0
31	18682.0
32	24019.0
33	32044.0
34	44174.0
35	43439.0
36	51483.0
37	66129.0
38	78756.0
39	38838.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.236081148809138	4.832755878058879	52.56825957159356	16.36290340153843
2	20.36041925432002	4.738183957638753	52.63668257392518	22.264714214116058
3	22.855243947615	5.282735176832058	50.466322372578496	21.39569850297444
4	20.208755529406638	5.282299361530583	46.5006210368046	28.00832407225818
5	20.27173084046981	5.910309210956396	48.94314789392256	24.874812054651237
6	22.502887276372274	7.431958336057179	51.48046457911137	18.584689808459174
7	85.15547710880129	2.606611318123379	8.390969906953433	3.8469416661218974
8	87.01945915321086	3.757817436970212	5.40062321588111	3.822100193937809
9	81.48198993266654	5.407378353053976	7.343269922206969	5.76736179207252
10	43.12109128151489	27.73746486239132	14.686975659715412	14.454468196378375
11	36.46749907389248	23.86764289294197	22.38434551437101	17.280512518794534
12	30.222701619053844	24.109956200562202	26.452681353642326	19.21466082674163
13	26.798500795362923	25.10884487154344	28.88409492057266	19.208559412520973
14	21.73585234577586	27.266130613845853	30.98298141247739	20.015035627900897
15	20.125514806824867	23.890087380967945	34.71552156196204	21.268876250245146
16	25.869342572617725	24.326120590093918	29.526486674947154	20.2780501623412
17	25.755158963631214	24.05417184197337	27.326055217798697	22.86461397659672
18	27.36353533372557	22.656947985443768	28.859907171340787	21.119609509489877
19	27.055631823233313	24.872415070493126	26.437427818090693	21.634525288182868
20	28.10660042274084	27.0769867730056	26.385347889564404	18.431064914689156
21	25.97698895208211	26.88413850210281	28.964284936044105	18.174587609770978
22	25.406942537752503	22.395894619860105	30.720184785687827	21.47697805669957
23	23.754766729859885	25.76649016146957	28.980628009849426	21.49811509882112
24	23.21936763199756	25.808328430411194	29.60057527619795	21.371728661393302
25	24.14678259353686	27.251966616547907	26.389052319626945	22.212198470288293
26	22.558453727310365	27.103135691094117	26.836416726591274	23.50199385500425
27	22.94131746965636	26.016866052167092	29.357390337974763	21.684426140201783
28	20.308339325793728	28.017694101239893	28.73243119565928	22.941535377307098
29	22.306334575406943	27.30557189862936	28.179817393388685	22.208276132575016
30	24.55623106927284	25.88873635353337	29.13381708831797	20.421215488875816
31	26.64051774857815	26.086160685101657	26.48623913185592	20.787082434464274
32	27.702381730622562	26.412368438255868	25.86040835893748	20.02484147218409
33	26.126255692837375	25.69610599028132	25.669739164542065	22.507899152339238
34	22.014556231069275	26.074829487263294	30.042927807195312	21.86768647447212
35	22.574360985814213	27.699766838813712	28.1179316205792	21.60794055479288
36	25.442025669521257	29.103310017214707	24.492166219955983	20.962498093308056
37	23.671308099627378	28.4742106295352	25.086400383517464	22.768080887319954
38	23.891176919221635	28.991741300037045	24.95718114663006	22.159900634111263
39	22.807304264452725	26.598679479636527	25.948878865136955	24.64513739077379
40	25.50587261118738	25.916846440478526	26.104247020112876	22.47303392822122
41	21.13028698437602	26.273779172386742	25.330456952343596	27.26547689089364
42	23.74082064021268	24.918829400100236	27.12078621080386	24.219563748883225
43	23.346189884726854	24.41088666623085	27.426074829487263	24.81684861955503
44	23.69767492536663	25.621363666078317	27.169815432219824	23.51114597633523
45	23.715107537425638	25.85757555947789	24.965679545008825	25.461637358087643
46	22.478917434791136	26.788477043428994	26.50824780458042	24.224357717199453
47	20.596849055370335	28.47268527598004	28.59079122267983	22.3396744459698
48	21.84589570939836	28.666840992787257	27.278333442287156	22.208929855527227
49	24.915778692989914	25.430040748730686	27.368547209692533	22.28563334858687
50	22.12568913294546	27.59037719814343	26.124512431631473	24.15942123727964
51	21.54191453661938	26.989823712710553	25.405417184197336	26.06284456647273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	197.5
2	321.0
3	562.0
4	803.0
5	635.5
6	468.0
7	528.0
8	588.0
9	605.5
10	623.0
11	624.5
12	626.0
13	679.0
14	732.0
15	774.0
16	816.0
17	820.0
18	824.0
19	1042.0
20	1260.0
21	1456.0
22	1652.0
23	1784.0
24	1916.0
25	2416.0
26	3809.0
27	4702.0
28	5584.5
29	6467.0
30	6891.5
31	7316.0
32	9088.5
33	10861.0
34	12215.5
35	13570.0
36	14066.0
37	14562.0
38	16907.0
39	19252.0
40	20124.0
41	20996.0
42	23126.0
43	25256.0
44	26297.0
45	27338.0
46	28562.0
47	29786.0
48	32780.5
49	35775.0
50	37525.0
51	39275.0
52	38317.0
53	37359.0
54	35617.5
55	33876.0
56	32009.5
57	30143.0
58	27604.0
59	25065.0
60	21859.0
61	18653.0
62	16362.0
63	14071.0
64	12492.0
65	10913.0
66	9168.0
67	7423.0
68	5848.0
69	4273.0
70	3718.5
71	3164.0
72	2623.0
73	2082.0
74	1733.0
75	1129.0
76	874.0
77	674.0
78	474.0
79	370.0
80	266.0
81	170.0
82	74.0
83	46.5
84	19.0
85	13.5
86	8.0
87	10.0
88	12.0
89	7.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.90958935578025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0733259412402	23.63649413001839
2	8.678595631433609	5.538608455678296
3	2.9633563779732306	2.836784554078743
4	1.6031953358886593	2.046292193012372
5	1.1211940041033095	1.78884201295498
6	0.8771601325615639	1.6793891777580765
7	0.7864610567142115	1.7566954554844445
8	0.7096000399673826	1.8114436705763541
9	0.6685170655377097	1.9198894534775597
>10	8.371222608448656	51.25195329091893
>50	0.10309107565068118	2.195859116276184
>100	0.03805375275696285	2.216170702712682
>500	0.006226977723866648	1.3215777870530099
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	847	0.18456778017476194	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC	800	0.17432612059009392	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	708	0.15427861672223311	No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	703	0.15318907846854501	Illumina PCR Primer Index 8 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	647	0.14098625002723844	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	595	0.12965505218888235	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	589	0.12834760628445666	Illumina PCR Primer Index 8 (95% over 24bp)
GCGCTCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	561	0.12224619206380337	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	536	0.11679850079536291	No Hit
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	497	0.10830010241659584	Illumina PCR Primer Index 8 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13946089647207513	0.0
2	0.0	0.0	0.0	0.5914013641018936	0.0
3	0.0	0.0	0.0	0.776840774879606	0.0
4	0.0	0.0	0.0	1.0823473012137457	0.0
5	0.0	0.0	0.0	1.6765814647752282	0.0
6	0.0	0.0	0.0	1.9781656533960907	0.0
7	0.0	0.0	0.0	2.2823647338258044	0.0
8	0.0	0.0	0.0	2.752609444117583	0.0
9	0.0	0.0	0.0	2.9709529101566754	0.0
10	0.0	0.0	0.0	3.55494541413349	0.0
11	0.0	0.0	0.0	4.3282996666012945	0.0
12	0.0	0.0	0.0	5.2509206598243665	0.0
13	0.0	0.0	0.0	5.496502582205661	0.0
14	0.0	0.0	0.0	5.603277331067094	0.0
15	0.0	0.0	0.0	5.78108997406899	0.0
16	0.0	0.0	0.0	6.134754091216142	0.0
17	0.0	0.0	0.0	6.654681745876098	0.0
18	0.0	0.0	0.0	7.228214682617507	0.0
19	0.0	0.0	0.0	7.666644875901593	0.0
20	0.0	0.0	0.0	8.114445098167396	0.0
21	0.0	0.0	0.0	8.67664683707045	0.0
22	0.0	0.0	0.0	9.268919831775294	0.0
23	0.0	0.0	0.0	9.841363230263015	0.0
24	0.0	0.0	0.0	10.282408315355951	0.0
25	0.0	0.0	0.0	10.70776404959578	0.0
26	0.0	0.0	0.0	11.08735917718071	0.0
27	0.0	0.0	0.0	11.508356758405787	0.0
28	0.0	0.0	0.0	11.928046893726439	0.0
29	0.0	0.0	0.0	12.405482556492558	0.0
30	0.0	0.0	0.0	13.047002680264104	0.0
31	0.0	0.0	0.0	13.502429670305725	0.0
32	0.0	0.0	0.0	13.96003573685472	0.0
33	0.0	0.0	0.0	14.4540323810769	0.0
34	0.0	0.0	0.0	14.977228650497919	0.0
35	0.0	0.0	0.0	15.552722756095967	0.0
36	0.0	0.0	0.0	16.07373994900961	0.0
37	0.0	0.0	0.0	16.57579917630908	0.0
38	0.0	0.0	0.0	17.19029875138916	0.0
39	0.0	0.0	0.0	18.20662003442941	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTC	20	7.0288515E-4	45.000004	39
TAGCGTC	20	7.0288515E-4	45.000004	10
TACGGGA	25	3.8871294E-5	45.000004	4
TACGAAT	25	3.8871294E-5	45.000004	12
TACGAAA	25	3.8871294E-5	45.000004	20
ATACGAA	25	3.8871294E-5	45.000004	19
GCCGTAC	20	7.0288515E-4	45.000004	31
TGGTCGA	20	7.0288515E-4	45.000004	14
CCGATGA	180	0.0	42.5	18
TCGCTTG	90	0.0	42.5	1
TAGCGGG	65	0.0	41.53846	3
CAATCGG	60	3.6379788E-12	41.249996	20
CGGGTAC	55	6.002665E-11	40.909092	6
CGACGCG	55	6.002665E-11	40.909092	13
GCGTCCC	55	6.002665E-11	40.909092	7
CGGGTAT	100	0.0	40.500004	6
CACCGCT	45	1.9239451E-8	40.0	38
TTTCTCG	210	0.0	39.642857	1
TCACGGG	40	3.452169E-7	39.375004	3
CGGGCTA	40	3.452169E-7	39.375004	6
>>END_MODULE