##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969197
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.92374512096096	31.0	30.0	31.0	25.0	34.0
2	30.042962369879394	31.0	30.0	33.0	25.0	34.0
3	30.114187311764276	31.0	30.0	33.0	25.0	34.0
4	33.90872443889116	35.0	33.0	37.0	28.0	37.0
5	34.39211223311669	35.0	35.0	37.0	32.0	37.0
6	33.78877668833065	35.0	35.0	37.0	29.0	37.0
7	34.33205426760504	35.0	35.0	37.0	32.0	37.0
8	34.311580617769145	35.0	35.0	37.0	31.0	37.0
9	35.820256356550836	37.0	35.0	39.0	31.0	39.0
10	35.32151358289388	37.0	34.0	39.0	30.0	39.0
11	35.43478260869565	37.0	35.0	39.0	30.0	39.0
12	35.40190178054616	37.0	35.0	39.0	30.0	39.0
13	35.31392585821045	37.0	35.0	39.0	30.0	39.0
14	35.913512939061924	38.0	34.0	40.0	27.0	41.0
15	36.15087541542122	38.0	34.0	40.0	29.0	41.0
16	36.379636957192396	38.0	35.0	40.0	30.0	41.0
17	36.10031603482058	38.0	34.0	40.0	30.0	41.0
18	36.27475528710881	38.0	35.0	40.0	30.0	41.0
19	36.227124103768375	38.0	34.0	40.0	30.0	41.0
20	36.151339717312375	38.0	34.0	40.0	30.0	41.0
21	35.915198870817804	38.0	34.0	40.0	29.0	41.0
22	36.12490649475803	38.0	34.0	40.0	30.0	41.0
23	36.16803188619032	38.0	34.0	40.0	30.0	41.0
24	36.10042437192851	38.0	34.0	40.0	30.0	41.0
25	35.706090712208145	38.0	34.0	40.0	29.0	41.0
26	35.79338256309089	38.0	34.0	40.0	29.0	41.0
27	35.77668833064898	38.0	34.0	40.0	29.0	41.0
28	35.61190449413277	38.0	34.0	40.0	29.0	41.0
29	35.720694554357884	38.0	34.0	40.0	29.0	41.0
30	35.24807030975127	38.0	34.0	40.0	27.0	41.0
31	35.37680471565636	38.0	34.0	40.0	28.0	41.0
32	35.35129803332037	38.0	34.0	40.0	27.0	41.0
33	35.29001224725211	38.0	34.0	40.0	27.0	41.0
34	35.175886842406655	38.0	34.0	40.0	27.0	41.0
35	35.20203322957046	38.0	34.0	40.0	27.0	41.0
36	34.96101721321878	37.0	33.0	40.0	26.0	41.0
37	34.95723779582479	37.0	33.0	40.0	26.0	41.0
38	34.78376325968817	37.0	33.0	40.0	25.0	41.0
39	34.72606601134754	37.0	33.0	40.0	26.0	41.0
40	34.606926146077626	37.0	33.0	40.0	25.0	41.0
41	34.53126247811333	37.0	33.0	40.0	25.0	41.0
42	34.73474948849408	37.0	33.0	40.0	26.0	41.0
43	34.70088021320743	37.0	33.0	40.0	26.0	41.0
44	34.717870567077696	37.0	33.0	40.0	26.0	41.0
45	34.70343490539075	37.0	33.0	40.0	26.0	41.0
46	34.60389992952929	36.0	33.0	40.0	26.0	41.0
47	34.47276043982802	36.0	33.0	40.0	26.0	41.0
48	34.469730096151764	36.0	33.0	40.0	26.0	41.0
49	34.46689888639771	36.0	33.0	40.0	26.0	41.0
50	34.24861302707293	36.0	33.0	40.0	26.0	41.0
51	33.15590741613934	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	6.0
11	14.0
12	15.0
13	25.0
14	39.0
15	77.0
16	147.0
17	304.0
18	545.0
19	1244.0
20	2329.0
21	3669.0
22	5293.0
23	7369.0
24	9467.0
25	11459.0
26	13412.0
27	16589.0
28	20257.0
29	25529.0
30	32359.0
31	40404.0
32	51499.0
33	66882.0
34	88895.0
35	91060.0
36	109724.0
37	136976.0
38	158003.0
39	75594.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.275675636635277	4.386105198427152	50.85550202899927	16.48271713593831
2	21.14750664725541	7.830502983397595	50.00417871702037	21.017811652326618
3	22.683210946794098	7.798311385610974	48.545032640422946	20.973445027171977
4	19.868303348029347	5.070279829590888	48.337128571384355	26.72428825099541
5	21.577450198463264	6.059758748737357	47.469296747720016	24.893494305079358
6	22.369239690176506	9.714330523103145	50.00624228098106	17.910187505739287
7	81.10683380159038	2.667672310170172	12.100429530838417	4.1250643574010235
8	82.7205408188428	6.027670329148769	6.899216567942328	4.352572284066087
9	77.43730118850966	4.9257271741451945	8.77086908027986	8.866102557065282
10	43.81833621028542	26.090774115066388	16.033995152688256	14.056894521959931
11	34.520742429041775	23.981399034458423	23.400196244932662	18.097662291567143
12	28.563542809150256	21.621816823617902	28.037540355572705	21.777100011659137
13	27.29775267566862	21.736241445237656	30.6361864512581	20.32981942783562
14	21.800830997206965	25.020713023255336	31.633197378861055	21.545258600676643
15	20.33487515953929	22.595819013059266	34.34688716535441	22.722418662047033
16	24.679090009564618	23.119551546280064	28.996994419091273	23.20436402506405
17	24.603769924999767	24.698900223587156	28.300232047767377	22.3970978036457
18	25.772366195933333	22.46932254226953	29.073243107438422	22.68506815435871
19	25.09004877233421	24.784434949757376	26.850578365389076	23.274937912519334
20	27.727902583272545	26.083448463005972	27.949735709045736	18.238913244675746
21	26.08396435399614	27.415994890615636	27.811476923680118	18.688563831708105
22	24.514830318294422	21.875532012583612	31.300034977409137	22.30960269171283
23	24.350776983420293	25.311159650721166	29.033416322997287	21.304647042861255
24	23.657832205423666	25.37079664918484	28.555907622495734	22.41546352289576
25	23.545574325962626	27.93642572149934	25.171146835988967	23.34685311654906
26	22.57704058101707	25.23749041732486	27.793523917222196	24.39194508443588
27	24.017924116562476	24.713757884104055	28.132154763169925	23.136163236163547
28	20.37501147857453	27.963974300374435	28.405576987960135	23.2554372330909
29	24.417945990340456	24.785054018945583	28.096970997640312	22.700028993073648
30	24.641429967282193	25.490999249894504	28.774129511337737	21.09344127148557
31	24.977584536477103	25.06146841147878	26.794552603856598	23.166394448187518
32	27.86843128899491	25.635551905340193	25.624099125358413	20.87191768030648
33	26.71706577713303	23.770605975874872	25.906910566169728	23.60541768082237
34	23.414434836261357	24.836024048774398	29.087584876965156	22.661956237999085
35	24.851603956677536	26.370696566332747	26.516384181956816	22.261315295032897
36	25.197663632883717	28.665379690609853	24.49935358858932	21.63760308791711
37	24.742544601355554	26.741106297274957	26.022366969769823	22.493982131599665
38	23.24305584932681	27.260918058970468	26.04052633262381	23.455499759078908
39	24.083751806908193	24.843143344438747	27.006790157212617	24.06631469144044
40	24.177953501713272	23.832203360101197	28.772581838367227	23.217261299818304
41	20.940737538395187	24.869453784937427	27.74699054990884	26.442818126758542
42	22.959418982931233	24.73831429523616	27.857700756399367	24.44456596543324
43	22.84757381626233	24.024940234028787	27.47635413646555	25.65113181324333
44	24.446423172997854	23.49512018712398	27.778047187517092	24.28040945236108
45	23.51451768835438	23.447348681434217	26.56013173792325	26.478001892288148
46	23.983153063825004	24.901026313535844	27.021750995927558	24.094069626711597
47	20.630480696906822	26.401340491148854	30.471823581789874	22.496355230154446
48	21.669278794713563	26.80487042366	28.45004679131281	23.07580399031363
49	23.852013574123735	24.148444537075537	29.038265698304887	22.961276190495845
50	21.982218269350813	24.898756393179095	27.881947632937372	25.237077704532723
51	21.479430910330922	24.326530106882295	27.26999774039746	26.92404124238932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	395.5
2	637.0
3	2267.5
4	3898.0
5	2795.0
6	1692.0
7	1685.0
8	1678.0
9	1761.0
10	1844.0
11	1815.5
12	1787.0
13	1793.5
14	1800.0
15	1807.5
16	1815.0
17	1958.5
18	2102.0
19	2102.5
20	2103.0
21	2347.5
22	2592.0
23	3055.0
24	3518.0
25	4288.0
26	6787.0
27	8516.0
28	9549.0
29	10582.0
30	13039.0
31	15496.0
32	17990.0
33	20484.0
34	23343.0
35	26202.0
36	27823.5
37	29445.0
38	31586.5
39	33728.0
40	36759.0
41	39790.0
42	42545.0
43	45300.0
44	49604.5
45	53909.0
46	68949.5
47	83990.0
48	84106.5
49	84223.0
50	82871.5
51	81520.0
52	77043.5
53	72567.0
54	69218.0
55	65869.0
56	63348.5
57	60828.0
58	57163.0
59	53498.0
60	47697.5
61	41897.0
62	37643.5
63	33390.0
64	30105.5
65	26821.0
66	22184.0
67	17547.0
68	14973.5
69	12400.0
70	10318.0
71	8236.0
72	6881.0
73	5526.0
74	4388.0
75	2586.0
76	1922.0
77	1372.5
78	823.0
79	663.5
80	504.0
81	337.5
82	171.0
83	115.5
84	60.0
85	33.0
86	6.0
87	10.0
88	14.0
89	7.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	969197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.57931026385228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23734953617075	23.443642941687866
2	8.657253573762546	5.467801932773827
3	2.958030576077946	2.8023769599578148
4	1.6089718016644172	2.032408789222001
5	1.1195597774009227	1.7677462784736562
6	0.8862582875174805	1.679245526125495
7	0.7963675698276209	1.760411700015954
8	0.7357262403269261	1.8586981770033237
9	0.6720127112730042	1.9099528119488502
>10	8.158071164899143	48.423127472822515
>50	0.11304668244991141	2.409340139042308
>100	0.05039026561649959	2.7219431339920357
>500	0.003315149053709569	0.866834198054729
>1k	0.0029836341483386116	1.7274916789977155
>5k	6.630298107419137E-4	1.128978259881908
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	5514	0.568924583959711	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	5270	0.5437491036394045	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	3548	0.3660762466247832	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2259	0.23307954935890227	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	2203	0.2273015702689959	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1962	0.2024356245427916	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1614	0.16652961162694477	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1550	0.15992620695276605	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1258	0.12979817312682562	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	1080	0.11143245387676604	No Hit
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1027	0.10596400938096176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14702893219851074	0.0
2	0.0	0.0	0.0	0.6846905221539068	0.0
3	0.0	0.0	0.0	0.9022933418077027	0.0
4	0.0	0.0	0.0	1.3401816142641796	0.0
5	0.0	0.0	0.0	2.3764002571200695	0.0
6	0.0	0.0	0.0	2.848750047719917	0.0
7	0.0	0.0	0.0	3.355251821869032	0.0
8	0.0	0.0	0.0	4.046545748697118	0.0
9	0.0	0.0	0.0	4.386105198427152	0.0
10	0.0	0.0	0.0	5.573479901402914	0.0
11	0.0	0.0	0.0	6.536648380050702	0.0
12	0.0	0.0	0.0	8.031390934969878	0.0
13	0.0	0.0	0.0	8.376934720185886	0.0
14	0.0	0.0	0.0	8.54140076785215	0.0
15	0.0	0.0	0.0	8.864245349500669	0.0
16	0.0	0.0	0.0	9.273243726507614	0.0
17	0.0	0.0	0.0	9.778404184082287	0.0
18	0.0	0.0	0.0	10.284183710845163	0.0
19	0.0	0.0	0.0	10.934515893053733	0.0
20	0.0	0.0	0.0	11.362499058498942	0.0
21	0.0	0.0	0.0	11.91625644734765	0.0
22	0.0	0.0	0.0	12.512523253786382	0.0
23	1.0317819803404261E-4	0.0	0.0	13.096924567451198	0.0
24	2.0635639606808522E-4	0.0	0.0	13.547710114661932	0.0
25	2.0635639606808522E-4	0.0	0.0	13.988590554861396	0.0
26	3.095345941021278E-4	0.0	0.0	14.37612786667726	0.0
27	3.095345941021278E-4	0.0	0.0	14.815047921114077	0.0
28	3.095345941021278E-4	0.0	0.0	15.21785560623898	0.0
29	3.095345941021278E-4	0.0	0.0	15.676895409292435	0.0
30	3.095345941021278E-4	0.0	0.0	16.2490185173912	0.0
31	3.095345941021278E-4	0.0	0.0	16.696296005868774	0.0
32	3.095345941021278E-4	0.0	0.0	17.15244681937728	0.0
33	4.1271279213617043E-4	0.0	0.0	17.604470504964418	0.0
34	4.1271279213617043E-4	0.0	0.0	18.091368421487065	0.0
35	4.1271279213617043E-4	0.0	0.0	18.64223682079082	0.0
36	4.1271279213617043E-4	0.0	0.0	19.1140707204005	0.0
37	4.1271279213617043E-4	0.0	0.0	19.592817559278455	0.0
38	4.1271279213617043E-4	0.0	0.0	20.153797421989545	0.0
39	4.1271279213617043E-4	0.0	0.0	21.039479073913764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCGA	145	0.0	41.89655	41
TACGGCT	800	0.0	40.78125	7
CGATGAA	1305	0.0	40.689655	19
CACTCGA	50	1.0804797E-9	40.5	41
CGATCGA	50	1.0804797E-9	40.5	41
TCGAATA	50	1.0804797E-9	40.5	44
CCGATGA	1330	0.0	39.924812	18
TTAATCG	40	3.4574805E-7	39.375	20
CGTTTTT	1605	0.0	38.971962	1
GATGAAT	1585	0.0	38.753944	20
GACCGAT	395	0.0	38.734177	9
TTTGGGC	1780	0.0	38.300564	4
CTCGAAT	160	0.0	37.96875	43
ATGAATG	1630	0.0	37.684048	21
CGATCAA	30	1.1397897E-4	37.500004	41
TGAATGA	1615	0.0	37.47678	22
TTTGGGA	3785	0.0	37.450462	4
TTGGGAC	1440	0.0	37.1875	5
ACGGCTG	890	0.0	37.16292	8
TCGAATG	140	0.0	36.964283	44
>>END_MODULE