SRR2934014.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181637
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1003	0.5522002675666301	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	823	0.4531015156603556	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	682	0.3754741600004404	Illumina PCR Primer Index 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	644	0.35455331237578247	Illumina PCR Primer Index 2 (95% over 22bp)
TGTCTGGGGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTA	264	0.14534483612920276	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	244	0.13433386369517225	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	223	0.1227723426394402	No Hit
TTTTTCGGGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTT	190	0.10460423812328987	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGGC	35	1.2043893E-7	45.000004	4
CTTGTCG	50	2.1827873E-11	45.0	1
CGGGTCT	20	7.0175895E-4	45.0	6
CGGGTAT	20	7.0175895E-4	45.0	6
CCGGGTA	20	7.0175895E-4	45.0	5
ACTTGAC	20	7.0175895E-4	45.0	41
TTGCTAG	20	7.0175895E-4	45.0	1
GGCGTTA	20	7.0175895E-4	45.0	20
TAGCGAA	20	7.0175895E-4	45.0	42
GGCAAAG	20	7.0175895E-4	45.0	8
GTTAGAC	20	7.0175895E-4	45.0	23
GTAGGCG	20	7.0175895E-4	45.0	17
TGACGGA	25	3.8777995E-5	45.0	20
GCGTTAG	20	7.0175895E-4	45.0	21
ACGGGAT	20	7.0175895E-4	45.0	5
CGTTAGA	20	7.0175895E-4	45.0	22
TACGAAA	25	3.8777995E-5	45.0	20
CTTGCAT	25	3.8777995E-5	45.0	30
TGACAAA	25	3.8777995E-5	45.0	11
GGAGTTA	20	7.0175895E-4	45.0	8