##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181637
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.06388015657603	31.0	30.0	31.0	26.0	34.0
2	30.415895439805766	31.0	30.0	33.0	27.0	34.0
3	30.42201203499287	31.0	30.0	34.0	26.0	34.0
4	34.07174749638014	35.0	33.0	37.0	30.0	37.0
5	34.46842328380231	35.0	35.0	37.0	32.0	37.0
6	34.0081866580047	35.0	35.0	37.0	30.0	37.0
7	34.5056954254915	35.0	35.0	37.0	32.0	37.0
8	34.55548153735197	35.0	35.0	37.0	32.0	37.0
9	35.855040547905986	37.0	35.0	39.0	30.0	39.0
10	35.302625566376896	37.0	34.0	39.0	30.0	39.0
11	35.480854671680326	37.0	35.0	39.0	30.0	39.0
12	35.34671350000275	37.0	34.0	39.0	30.0	39.0
13	35.20066396163777	37.0	34.0	39.0	30.0	39.0
14	35.82297109069187	38.0	34.0	40.0	27.0	41.0
15	36.194360179919286	38.0	34.0	40.0	30.0	41.0
16	36.41122678749374	38.0	35.0	40.0	31.0	41.0
17	36.21073349592869	38.0	34.0	40.0	30.0	41.0
18	36.28531081222438	38.0	35.0	40.0	30.0	41.0
19	36.3019483915722	38.0	34.0	40.0	30.0	41.0
20	36.366125844403946	38.0	34.0	40.0	30.0	41.0
21	36.03469557413963	38.0	34.0	40.0	30.0	41.0
22	36.182721582056516	38.0	34.0	40.0	30.0	41.0
23	36.3335939263476	38.0	34.0	40.0	30.0	41.0
24	36.282756266619685	38.0	35.0	40.0	30.0	41.0
25	35.809680846964	38.0	34.0	40.0	29.0	41.0
26	35.900983830386984	38.0	34.0	40.0	30.0	41.0
27	35.98930834576656	38.0	34.0	40.0	30.0	41.0
28	35.851517036727095	38.0	34.0	40.0	29.0	41.0
29	36.01458403298887	38.0	34.0	40.0	30.0	41.0
30	35.56533635768043	38.0	34.0	40.0	29.0	41.0
31	35.686346944730424	38.0	34.0	40.0	29.0	41.0
32	35.76311544454048	38.0	34.0	40.0	29.0	41.0
33	35.76132065603374	38.0	34.0	40.0	29.0	41.0
34	35.75768152964429	38.0	34.0	40.0	29.0	41.0
35	35.820752379746416	38.0	34.0	40.0	29.0	41.0
36	35.63701778822597	38.0	34.0	40.0	29.0	41.0
37	35.660592280207226	38.0	34.0	40.0	29.0	41.0
38	35.57128778828102	38.0	34.0	40.0	29.0	41.0
39	35.560926463220596	38.0	34.0	40.0	29.0	41.0
40	35.471632982266826	38.0	34.0	40.0	29.0	41.0
41	35.37223693410483	37.0	34.0	40.0	28.0	41.0
42	35.624685499099854	38.0	34.0	40.0	29.0	41.0
43	35.58582777738016	38.0	34.0	40.0	29.0	41.0
44	35.53873384827981	37.0	34.0	40.0	29.0	41.0
45	35.619851682201315	38.0	34.0	40.0	29.0	41.0
46	35.500624872685634	37.0	34.0	40.0	29.0	41.0
47	35.44520114293894	37.0	34.0	40.0	29.0	41.0
48	35.50037712580586	37.0	34.0	40.0	29.0	41.0
49	35.532655791496225	37.0	34.0	40.0	29.0	41.0
50	35.386760406745324	37.0	34.0	40.0	29.0	41.0
51	34.26120228808007	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	4.0
12	3.0
13	2.0
14	7.0
15	9.0
16	14.0
17	33.0
18	64.0
19	115.0
20	208.0
21	327.0
22	466.0
23	752.0
24	1026.0
25	1466.0
26	1861.0
27	2541.0
28	3283.0
29	4244.0
30	5528.0
31	7295.0
32	9411.0
33	12720.0
34	18037.0
35	17873.0
36	20825.0
37	26627.0
38	31773.0
39	15116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.55817922559831	4.884467371735935	55.382438600064965	17.174914802600792
2	21.3282536047171	3.9810170835237315	53.39605917296586	21.294670138793308
3	21.6172916311104	4.097182842702753	52.836151224695406	21.449374301491435
4	20.054834642721474	4.778211487747541	47.5277614142493	27.639192455281687
5	18.332168005417397	5.785164916839631	51.285255757362215	24.59741132038076
6	22.524595759674515	6.626403210799562	52.185953302465904	18.663047727060015
7	83.2875460396285	2.4752666031700588	9.134152182650011	5.10303517455144
8	83.48023805722403	3.670507660884071	7.009034502882122	5.840219779009783
9	76.60113302906346	6.364342066869636	10.369583289748235	6.664941614318669
10	39.52608774643932	27.48944323017887	17.772260057146948	15.212208966234853
11	33.26084443147597	24.806619796627338	24.436651122843912	17.49588464905278
12	29.963608736105527	21.409184252107224	29.76871452402319	18.858492487764057
13	23.65597317727115	27.930983224783496	29.424071086838033	18.98897251110732
14	19.526307965888005	29.496192956280936	29.066214482732043	21.911284595099016
15	16.163556984535088	26.356414166717133	38.44315860755243	19.03687024119535
16	19.533465097970126	23.477044875218155	35.169596502915155	21.819893523896564
17	19.790020755683038	23.156625577387867	29.513810512175382	27.53954315475371
18	21.193369192400226	23.784251006127608	31.813452105022655	23.20892769644951
19	24.24395910524838	25.471131983021078	28.18863997973981	22.09626893199073
20	27.490544327422278	26.13784636390163	28.411612171528926	17.95999713714717
21	24.612826681788402	27.2818863997974	27.212517273463003	20.892769644951194
22	22.15187434278258	25.32083220929656	28.303154093053728	24.224139354867123
23	21.366792008236207	26.633340123433	27.67497811569229	24.324889752638505
24	22.97714672671317	23.390608741611015	29.833128712762267	23.799115818913545
25	19.52795961175311	25.59225267979542	29.237435104081218	25.642352604370256
26	18.893177050931254	28.277828856455457	29.710906918744527	23.11808717386876
27	21.397622731051495	28.316367259974562	28.72872817762901	21.557281831344934
28	18.276012046003842	27.504308042964816	32.50879501423169	21.710884896799662
29	18.45108650770493	25.27899051404725	32.99933383616774	23.270589142080084
30	21.079405627708013	26.832638724488955	30.47286621117944	21.615089436623595
31	22.827948050232056	28.82287199193997	26.71647296530993	21.632706992518045
32	22.68590650583306	27.08368889598485	27.169023932348583	23.061380665833504
33	22.419440972929525	26.761617952289456	27.678281407422496	23.140659667358523
34	20.10548511591801	25.815224871584537	29.716412404961545	24.36287760753591
35	19.567599112515623	24.589153091055234	30.056100904551386	25.787146891877754
36	21.7604342727528	26.435693168242153	28.937386105253886	22.866486453751165
37	20.442971421021046	28.839388450591013	29.15650445669109	21.561135671696846
38	19.475657492691468	28.182033396279394	28.06696873434377	24.275340376685367
39	19.784515269466024	25.73429422419441	31.02781922185458	23.453371284484987
40	20.795873087531724	25.44855948953132	29.917362651882602	23.838204771054354
41	17.391830959551193	25.740900807654825	28.251402522613784	28.615865710180195
42	20.39672533679812	24.019885816215858	27.74875163100029	27.83463721598573
43	21.5798543248347	23.848665194866683	27.830232827012118	26.7412476532865
44	19.483915722016988	24.98389645281523	29.62447078513739	25.90771704003039
45	19.765246067706467	25.40947053739051	28.54979987557601	26.275483519327008
46	21.020496925185945	26.28979778349125	29.950946117806392	22.73875917351641
47	17.869156614566414	26.18629464261136	31.67801714408408	24.26653159873814
48	18.652036754625982	23.447315249646273	31.497987744787682	26.402660250940063
49	19.747077963190318	22.4827540644252	33.242125778338114	24.528042194046368
50	19.45859048541872	23.490808590760693	31.159950891062945	25.890650032757645
51	17.258598193099424	24.18670204859142	28.117068658918615	30.437631099390543
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	96.0
2	159.0
3	233.5
4	308.0
5	251.0
6	194.0
7	209.0
8	224.0
9	281.5
10	339.0
11	328.0
12	317.0
13	341.5
14	366.0
15	371.0
16	376.0
17	357.5
18	339.0
19	388.5
20	438.0
21	466.0
22	494.0
23	618.5
24	743.0
25	845.0
26	1213.5
27	1480.0
28	2090.0
29	2700.0
30	2771.5
31	2843.0
32	3370.5
33	3898.0
34	4480.0
35	5062.0
36	5499.5
37	5937.0
38	6684.5
39	7432.0
40	9045.5
41	10659.0
42	12471.5
43	14284.0
44	16168.5
45	18053.0
46	19459.0
47	20865.0
48	19978.5
49	19092.0
50	16587.5
51	14083.0
52	12007.5
53	9932.0
54	9271.5
55	8611.0
56	7932.5
57	7254.0
58	6993.0
59	6732.0
60	6228.0
61	5724.0
62	4924.0
63	4124.0
64	3403.0
65	2682.0
66	2288.0
67	1894.0
68	1515.0
69	1136.0
70	943.5
71	751.0
72	641.0
73	531.0
74	432.0
75	232.5
76	132.0
77	82.0
78	32.0
79	45.5
80	59.0
81	37.0
82	15.0
83	21.5
84	28.0
85	15.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	181637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.65711831840429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.00877387827633	29.34974702290833
2	10.294035984007108	8.164636059833624
3	3.609506885828521	4.2942792492718995
4	1.9741226121723676	3.1315205602382776
5	1.2397267880941805	2.4581995958973115
6	0.9926143936028432	2.3618535870995445
7	0.8163038649489116	2.2660581269234794
8	0.7191248334073745	2.281473488331122
9	0.6510995113282986	2.3238657322021394
>10	5.5877943136383825	37.90086821517642
>50	0.06941359395824077	1.871865313785187
>100	0.03193025322079076	1.860303792729455
>500	0.0041648156374944475	1.1831289880365783
>1k	0.0013882718791648156	0.5522002675666301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1003	0.5522002675666301	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	823	0.4531015156603556	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	682	0.3754741600004404	Illumina PCR Primer Index 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	644	0.35455331237578247	Illumina PCR Primer Index 2 (95% over 22bp)
TGTCTGGGGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTA	264	0.14534483612920276	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	244	0.13433386369517225	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	223	0.1227723426394402	No Hit
TTTTTCGGGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTT	190	0.10460423812328987	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4888871760709547	0.0
2	0.0	0.0	0.0	1.9170103007647121	0.0
3	0.0	0.0	0.0	2.4752666031700588	0.0
4	0.0	0.0	0.0	3.3324708071593343	0.0
5	0.0	0.0	0.0	4.756740091501181	0.0
6	0.0	0.0	0.0	5.462543424522536	0.0
7	0.0	0.0	0.0	6.194773091385565	0.0
8	0.0	0.0	0.0	7.363037266636203	0.0
9	0.0	0.0	0.0	7.877800227927129	0.0
10	0.0	0.0	0.0	9.00036886757654	0.0
11	0.0	0.0	0.0	10.87663857033534	0.0
12	0.0	0.0	0.0	12.776031315205602	0.0
13	0.0	0.0	0.0	13.339793103827965	0.0
14	0.0	0.0	0.0	13.508811530690332	0.0
15	0.0	0.0	0.0	13.888690079664386	0.0
16	0.0	0.0	0.0	14.705704234269449	0.0
17	0.0	0.0	0.0	16.025369280488007	0.0
18	0.0	0.0	0.0	17.514052753568933	0.0
19	0.0	0.0	0.0	18.323359227470174	0.0
20	0.0	0.0	0.0	19.175057945242433	0.0
21	0.0	0.0	0.0	20.257987084129336	0.0
22	0.0	0.0	0.0	21.33926457715113	0.0
23	0.0	0.0	0.0	22.36328501351597	0.0
24	0.0	0.0	0.0	23.144513507710432	0.0
25	0.0	0.0	0.0	23.88334975803388	0.0
26	0.0	0.0	0.0	24.53630042337189	0.0
27	0.0	0.0	0.0	25.126488545835926	0.0
28	0.0	0.0	0.0	25.754664523197366	0.0
29	0.0	0.0	0.0	26.427985487538333	0.0
30	0.0	0.0	0.0	27.265920489768053	0.0
31	0.0	0.0	0.0	28.037789657393592	0.0
32	0.0	0.0	0.0	28.766716032526414	0.0
33	0.0	0.0	0.0	29.477474303143083	0.0
34	5.505486217015255E-4	0.0	0.0	30.182727087542737	0.0
35	5.505486217015255E-4	0.0	0.0	30.950191866194665	0.0
36	5.505486217015255E-4	0.0	0.0	31.661500685433033	0.0
37	5.505486217015255E-4	0.0	0.0	32.352439205668446	0.0
38	5.505486217015255E-4	0.0	0.0	33.04502937176897	0.0
39	5.505486217015255E-4	0.0	0.0	33.77505684414519	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGGC	35	1.2043893E-7	45.000004	4
CTTGTCG	50	2.1827873E-11	45.0	1
CGGGTCT	20	7.0175895E-4	45.0	6
CGGGTAT	20	7.0175895E-4	45.0	6
CCGGGTA	20	7.0175895E-4	45.0	5
ACTTGAC	20	7.0175895E-4	45.0	41
TTGCTAG	20	7.0175895E-4	45.0	1
GGCGTTA	20	7.0175895E-4	45.0	20
TAGCGAA	20	7.0175895E-4	45.0	42
GGCAAAG	20	7.0175895E-4	45.0	8
GTTAGAC	20	7.0175895E-4	45.0	23
GTAGGCG	20	7.0175895E-4	45.0	17
TGACGGA	25	3.8777995E-5	45.0	20
GCGTTAG	20	7.0175895E-4	45.0	21
ACGGGAT	20	7.0175895E-4	45.0	5
CGTTAGA	20	7.0175895E-4	45.0	22
TACGAAA	25	3.8777995E-5	45.0	20
CTTGCAT	25	3.8777995E-5	45.0	30
TGACAAA	25	3.8777995E-5	45.0	11
GGAGTTA	20	7.0175895E-4	45.0	8
>>END_MODULE