FastQCFastQC Report
Sat 14 Jan 2017
SRR2934013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708982
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC32510.45854478675058047No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG24180.34105238214792477No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC19010.268130925749878No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC13030.18378463769178907TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11640.16417906237393898No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT11400.1607939270672598No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC10700.1509206157561123TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT9320.13145608774270715TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC8040.11340203277375165TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC7880.11114527590263222No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.10522128911594371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG351.2110468E-745.0000041
TATTGCG302.164319E-645.0000041
TACGCGG406.8084773E-945.02
AACGCGT207.031455E-445.013
CGAAATG253.8892893E-544.99999615
CTACGCG253.8892893E-544.9999961
GCGATAT253.8892893E-544.9999969
CGATCCA253.8892893E-544.99999620
CGTCATA502.1827873E-1144.99999638
CGTTTGG4750.041.2105262
GCGTCAT501.0804797E-940.49999637
ACACGCG4650.040.16129336
CGCCCAC451.9264917E-840.034
TACACGC4850.039.89690835
TTTCGCG2500.039.6000021
TTACACG4900.039.48979634
CACGCGG4800.038.90625437
CGATGAA5650.038.62831519
TTACTCG356.2449017E-638.571431
CCCCGCT700.038.5714338
Produced by FastQC (version 0.11.5)