##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934012.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	111545
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.217598278721592	31.0	30.0	31.0	27.0	34.0
2	30.554699896902594	31.0	30.0	33.0	27.0	34.0
3	30.64871576493792	31.0	31.0	34.0	27.0	34.0
4	34.19429826527411	35.0	33.0	37.0	30.0	37.0
5	34.542032363620066	35.0	35.0	37.0	32.0	37.0
6	34.11734277645793	35.0	35.0	37.0	30.0	37.0
7	34.495163386973864	35.0	35.0	37.0	32.0	37.0
8	34.833331839168046	35.0	35.0	37.0	32.0	37.0
9	36.09716258012461	38.0	35.0	39.0	31.0	39.0
10	35.445093908288136	37.0	35.0	39.0	30.0	39.0
11	35.488869962795285	37.0	35.0	39.0	30.0	39.0
12	35.22520955668116	37.0	34.0	39.0	30.0	39.0
13	34.96192568021875	37.0	34.0	39.0	27.0	39.0
14	35.58997713927115	38.0	34.0	40.0	27.0	41.0
15	36.088654802994306	38.0	34.0	40.0	30.0	41.0
16	36.309668743556415	38.0	34.0	40.0	31.0	41.0
17	36.179335694114485	38.0	34.0	40.0	30.0	41.0
18	36.244959433412525	38.0	35.0	40.0	30.0	41.0
19	36.21126899457618	38.0	35.0	40.0	30.0	41.0
20	36.280550450490836	38.0	34.0	40.0	30.0	41.0
21	35.917405531399886	38.0	34.0	40.0	30.0	41.0
22	36.07788784795374	38.0	34.0	40.0	30.0	41.0
23	36.214299161773276	38.0	34.0	40.0	30.0	41.0
24	36.13671612353759	38.0	34.0	40.0	30.0	41.0
25	35.66487964498633	37.0	34.0	40.0	29.0	41.0
26	35.751015285310864	38.0	34.0	40.0	30.0	41.0
27	35.763028374198754	38.0	34.0	40.0	30.0	41.0
28	35.6250123268636	37.0	34.0	40.0	29.0	41.0
29	35.8259895109597	38.0	34.0	40.0	30.0	41.0
30	35.3798377336501	37.0	34.0	40.0	29.0	41.0
31	35.51158725178179	37.0	34.0	40.0	29.0	41.0
32	35.57331121968712	37.0	34.0	40.0	29.0	41.0
33	35.562696669505584	37.0	34.0	40.0	29.0	41.0
34	35.584768479089156	37.0	34.0	40.0	29.0	41.0
35	35.55827692859384	37.0	34.0	40.0	29.0	41.0
36	35.34191581872787	37.0	34.0	40.0	29.0	41.0
37	35.32706979246044	37.0	34.0	40.0	29.0	41.0
38	35.18853377560626	37.0	34.0	40.0	28.0	41.0
39	35.19373347079654	37.0	34.0	40.0	28.0	41.0
40	35.07144201891613	37.0	34.0	40.0	28.0	41.0
41	35.00742301313371	37.0	34.0	40.0	28.0	41.0
42	35.21484602626742	37.0	34.0	40.0	29.0	41.0
43	35.173867049172976	37.0	34.0	40.0	29.0	41.0
44	35.080729750324984	36.0	34.0	40.0	28.0	41.0
45	35.18631045766283	36.0	34.0	40.0	29.0	41.0
46	35.07935810659375	36.0	34.0	40.0	28.0	41.0
47	35.0250212918553	36.0	34.0	40.0	28.0	41.0
48	34.997095342686805	36.0	34.0	40.0	28.0	41.0
49	35.0348469227666	36.0	34.0	40.0	28.0	41.0
50	34.84813304047694	36.0	34.0	39.0	28.0	41.0
51	33.70753507553005	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	2.0
14	2.0
15	7.0
16	16.0
17	25.0
18	42.0
19	81.0
20	136.0
21	226.0
22	370.0
23	493.0
24	719.0
25	1033.0
26	1257.0
27	1673.0
28	2083.0
29	2750.0
30	3452.0
31	4469.0
32	6098.0
33	8150.0
34	12372.0
35	11596.0
36	12046.0
37	15341.0
38	18312.0
39	8784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.004079071226858	4.662692187009727	57.21457707651621	17.11865166524721
2	19.367071585458785	3.778744004661796	57.217266574028415	19.636917835851
3	21.126899457618002	3.3116679367071584	53.78995024429602	21.771482361378816
4	19.84400914429154	4.521045318033081	48.90313326460173	26.731812273073647
5	16.980590792953517	6.0029584472634365	51.680487695548884	25.335963064234168
6	21.229996862252904	6.142812317898605	54.44349813976422	18.18369268008427
7	86.56147743063337	2.075395580259088	7.427495629566542	3.935631359540993
8	88.95961271235824	1.9445067013313013	5.39602850867363	3.6998520776368284
9	83.8253619615402	4.938813931597114	6.773051234927607	4.462772871935093
10	47.716168362544266	25.145905240037653	12.905105562777356	14.232820834640728
11	36.5583396835358	24.161549150567037	20.515487023174504	18.76462414272267
12	32.76435519297144	21.10090098166659	26.70222780043928	19.432516024922677
13	23.437177820610515	29.283248912994758	28.51136312698911	18.76821013940562
14	18.440091442915417	31.01169931417813	30.354565422026987	20.193643820879466
15	16.68295306826841	24.901160966426104	39.66650230848536	18.749383656820118
16	19.89242009951141	21.85396028508674	36.85508090905016	21.398538706351697
17	21.182482406203775	20.799677260298534	28.388542740597966	29.62929759289973
18	22.880451835582054	22.12649603299117	30.995562329104846	23.997489802321933
19	25.80034963467659	23.179882558608632	27.535972029225874	23.483795777488904
20	29.12098256309113	24.213546102469856	26.08274687345914	20.582724460979872
21	25.776144157066653	25.143215742525438	27.395221659419967	21.68541844098794
22	24.323815500470662	23.593168676318975	27.906226186740778	24.176789636469586
23	24.295127527007036	25.230176162087048	25.357479044331882	25.117217266574027
24	23.941010354565424	24.234165583396834	28.862790801918507	22.962033260119235
25	20.812228248688868	24.78551257340087	28.83410282845488	25.56815634945538
26	21.22013537137478	29.51544219821597	27.811197274642517	21.45322515576673
27	21.948989197184993	28.465641669281457	27.19619884351607	22.389170290017482
28	19.279214666726432	27.76995831278856	31.705589672329555	21.245237348155452
29	20.069926935317582	24.94598592496302	32.55278138867722	22.43130575104218
30	22.139943520552244	26.320319153704787	28.91120175713838	22.628535568604597
31	26.319422654534048	26.284459186875253	24.58380025998476	22.812317898605944
32	26.391142588193105	27.007934017661032	25.303689094087588	21.297234300058275
33	25.983235465507192	26.889596127123582	25.338652561746382	21.788515845622843
34	22.707427495629567	26.889596127123582	26.780223228293515	23.622753148953336
35	21.601147518938546	26.26563270428975	28.657492491819447	23.47572728495226
36	26.754224752342104	23.968801828858307	27.384463669371108	21.89250974942848
37	24.22699359003093	27.492043569859696	27.743063337666413	20.53789950244296
38	22.35779281904164	28.672732977721992	25.09928728315926	23.8701869200771
39	24.65641669281456	26.372316105607602	27.1899233493209	21.78134385225694
40	24.3973284324712	25.258864135550674	27.16661437088171	23.177193061096418
41	20.191850822537987	25.16921421847685	28.07476803083957	26.564166928145593
42	23.89977139271146	24.284369536958177	26.807118203415666	25.008740866914696
43	24.11313819534717	24.234165583396834	27.266125778833654	24.38657044242234
44	21.811824824062036	26.972074050831502	27.159442377515802	24.05665874759066
45	21.76341386884217	27.05903447039312	25.67573624994397	25.501815410820743
46	23.135954099242458	25.93482451028733	28.44681518669595	22.48240620377426
47	21.11793446591062	25.810211125554712	29.170290017481733	23.901564391052936
48	22.734322470751714	23.85404993500381	29.64991707382671	23.76171052041777
49	23.39952485543951	22.76390694338608	30.246089022367656	23.59047917880676
50	22.09153256533238	24.54345779730154	29.10574207718858	24.25926756017751
51	21.439777668205657	25.981442467165717	26.438657044242238	26.14012282038639
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	54.0
2	83.0
3	138.5
4	194.0
5	152.0
6	110.0
7	119.5
8	129.0
9	170.5
10	212.0
11	227.0
12	242.0
13	250.5
14	259.0
15	238.5
16	218.0
17	187.5
18	157.0
19	217.5
20	278.0
21	338.5
22	399.0
23	448.0
24	497.0
25	535.5
26	737.5
27	901.0
28	1004.5
29	1108.0
30	1313.0
31	1518.0
32	1766.5
33	2015.0
34	2370.0
35	2725.0
36	2914.5
37	3104.0
38	3467.5
39	3831.0
40	4642.5
41	5454.0
42	6690.0
43	7926.0
44	8342.5
45	8759.0
46	9180.0
47	9601.0
48	9927.0
49	10253.0
50	10206.0
51	10159.0
52	8981.0
53	7803.0
54	7150.5
55	6498.0
56	6280.5
57	6063.0
58	5499.5
59	4936.0
60	4847.0
61	4758.0
62	4227.5
63	3697.0
64	3106.5
65	2516.0
66	2162.0
67	1808.0
68	1524.0
69	1240.0
70	1009.5
71	779.0
72	565.5
73	352.0
74	269.5
75	148.0
76	109.0
77	66.5
78	24.0
79	31.0
80	38.0
81	21.5
82	5.0
83	3.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	111545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.04684208167107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.25899777453763	26.375902102290556
2	8.119100606246644	5.69097673584652
3	2.7345048985751923	2.8750728405576225
4	1.4171335021615123	1.9866421623559998
5	1.0283170900161154	1.8019633331839167
6	1.0283170900161154	2.1623559998207
7	0.8032128514056224	1.970505177282711
8	0.7136827565037219	2.000986149087812
9	0.7162407592152048	2.259177910260433
>10	8.083288568285882	49.44282576538617
>50	0.07674008134448622	1.8019633331839167
>100	0.020464021691862993	1.631628490743646
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	481	0.43121610112510644	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	302	0.27074274956295663	TruSeq Adapter, Index 15 (95% over 22bp)
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	290	0.25998475951409744	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	210	0.1882648258550361	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	164	0.1470258640010758	TruSeq Adapter, Index 14 (95% over 21bp)
TTTGTAGGGAAGAAAGAAGAAAAGAAAAGGCATAAGTCCTCGTCCTCCTCC	134	0.1201308888789278	No Hit
TTTCTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	130	0.11654489219597472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30122372136805775	0.0
2	0.0	0.0	0.0	1.1708279169841769	0.0
3	0.0	0.0	0.0	1.4756376350351876	0.0
4	0.0	0.0	0.0	1.94002420547761	0.0
5	0.0	0.0	0.0	2.6410865569949347	0.0
6	0.0	0.0	0.0	3.048097180510108	0.0
7	0.0	0.0	0.0	3.406696848805415	0.0
8	0.0	0.0	0.0	4.040521762517369	0.0
9	0.0	0.0	0.0	4.289748531982608	0.0
10	0.0	0.0	0.0	4.782823075888655	0.0
11	0.0	0.0	0.0	5.86131157828679	0.0
12	0.0	0.0	0.0	6.837599175220763	0.0
13	0.0	0.0	0.0	7.187233851808687	0.0
14	0.0	0.0	0.0	7.28943475727285	0.0
15	0.0	0.0	0.0	7.473217087274194	0.0
16	0.0	0.0	0.0	7.964498632838764	0.0
17	0.0	0.0	0.0	8.700524452014882	0.0
18	0.0	0.0	0.0	9.681294544802546	0.0
19	0.0	0.0	0.0	10.209332556367386	0.0
20	0.0	0.0	0.0	10.809090501591285	0.0
21	0.0	0.0	0.0	11.527186337352639	0.0
22	0.0	0.0	0.0	12.234524183065131	0.0
23	0.0	0.0	0.0	12.856694607557488	0.0
24	0.0	0.0	0.0	13.383836119951589	0.0
25	0.0	0.0	0.0	13.837464700345151	0.0
26	0.0	0.0	0.0	14.274956295665426	0.0
27	0.0	0.0	0.0	14.675691424985432	0.0
28	0.0	0.0	0.0	15.108700524452015	0.0
29	0.0	0.0	0.0	15.565018602357792	0.0
30	0.0	0.0	0.0	16.092160114751895	0.0
31	0.0	0.0	0.0	16.581648661974988	0.0
32	0.0	0.0	0.0	17.055896723295533	0.0
33	0.0	0.0	0.0	17.509525303689095	0.0
34	0.0	0.0	0.0	17.980187368326686	0.0
35	0.0	0.0	0.0	18.479537406427898	0.0
36	0.0	0.0	0.0	18.983369940382804	0.0
37	0.0	0.0	0.0	19.48182347931328	0.0
38	0.0	0.0	0.0	19.95338204312161	0.0
39	0.0	0.0	0.0	20.451835582052087	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	25	3.8681086E-5	45.000004	30
CGGGTAT	25	3.8681086E-5	45.000004	6
CGACGAC	25	3.8681086E-5	45.000004	35
TCGGGAT	30	2.1478481E-6	45.000004	5
TGCGGGC	30	2.1478481E-6	45.000004	4
CGGGGTT	25	3.8681086E-5	45.000004	6
CGGGCTC	30	2.1478481E-6	45.000004	6
GTATCTA	30	2.1478481E-6	45.000004	24
GATGCCA	25	3.8681086E-5	45.000004	9
ATTGGGA	30	2.1478481E-6	45.000004	4
GAATTAA	25	3.8681086E-5	45.000004	44
ACGACAT	25	3.8681086E-5	45.000004	37
GTAGTCA	25	3.8681086E-5	45.000004	10
GGTAGTC	25	3.8681086E-5	45.000004	9
CTGTAAC	20	7.005885E-4	45.0	14
TTTCGCG	45	3.783498E-10	45.0	1
TGGGTCC	35	1.1987868E-7	45.0	6
TGTGGGC	45	3.783498E-10	45.0	4
TGGGTAC	20	7.005885E-4	45.0	6
GTAGGAT	20	7.005885E-4	45.0	13
>>END_MODULE