FastQCFastQC Report
Sat 14 Jan 2017
SRR2934010.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934010.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314462
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC41981.3349784711666275No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG33851.0764416686276879No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC23680.7530321628686455TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT16180.5145295775006201No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA9100.2893831369132041No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC8060.256310778408838TruSeq Adapter, Index 22 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT6740.2143343233840655No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC6640.21115428891249183No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC5830.18539600969274506TruSeq Adapter, Index 22 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT5640.1793539441967551No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT5590.17776392696096827Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCGAATGC4400.13992151674924155No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3930.12497535473284531No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3730.11861528578969796No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC3700.11766127544822587No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC3510.11161920995223588TruSeq Adapter, Index 22 (95% over 24bp)
TTTTCCGGGACATCTTCTATGAAAAAACTTTCTACCCCTAACACTAGCATT3390.10780316858634749No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGT3380.10748516513919011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAC302.1604537E-645.00000430
ACGGCTC600.045.00000421
CACCGCG650.045.00000438
TTGATAG207.025463E-445.031
GTCTGCG207.025463E-445.01
CCGATCG351.2081728E-745.040
ACACCGA207.025463E-445.037
CAGCATA207.025463E-445.020
CCGTGCA207.025463E-445.041
GTTAGGG700.045.03
TACGGAG453.8198777E-1045.029
CGTTCGG253.8843227E-545.02
TTATCGG207.025463E-445.02
AGAACCC207.025463E-445.010
ACGATTA207.025463E-445.012
TTAAGGG253.8843227E-545.03
TACGAAT453.8198777E-1045.012
TTGACGG207.025463E-445.02
CGTACAA207.025463E-445.010
ATACGGA453.8198777E-1045.028
Produced by FastQC (version 0.11.5)