##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934010.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314462
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83498483123557	31.0	30.0	31.0	25.0	34.0
2	29.547439754246938	31.0	30.0	33.0	25.0	34.0
3	29.66604549993322	31.0	30.0	33.0	25.0	34.0
4	33.771342801355964	35.0	33.0	37.0	28.0	37.0
5	34.3954690868849	35.0	35.0	37.0	32.0	37.0
6	33.54516284956529	35.0	33.0	37.0	28.0	37.0
7	34.298970940844995	35.0	35.0	37.0	32.0	37.0
8	33.83523605395882	35.0	35.0	37.0	28.0	37.0
9	35.65998117419593	37.0	35.0	39.0	30.0	39.0
10	35.29121483676883	37.0	34.0	39.0	30.0	39.0
11	35.43848541318188	37.0	35.0	39.0	30.0	39.0
12	35.38205570148381	37.0	35.0	39.0	30.0	39.0
13	35.204752879521216	37.0	34.0	39.0	29.0	39.0
14	35.959470460659794	38.0	34.0	40.0	29.0	41.0
15	36.22247521163129	38.0	34.0	40.0	30.0	41.0
16	36.52907823520807	38.0	35.0	40.0	31.0	41.0
17	36.171047694157004	38.0	34.0	40.0	30.0	41.0
18	36.35364845354923	38.0	35.0	40.0	30.0	41.0
19	36.277966813160255	38.0	35.0	40.0	30.0	41.0
20	36.07075576699251	38.0	34.0	40.0	29.0	41.0
21	35.999185911175275	38.0	34.0	40.0	30.0	41.0
22	36.161889194878874	38.0	34.0	40.0	30.0	41.0
23	36.04010341472102	38.0	34.0	40.0	30.0	41.0
24	36.013372044952966	38.0	34.0	40.0	30.0	41.0
25	35.73869338743632	38.0	34.0	40.0	29.0	41.0
26	35.773966329795016	38.0	34.0	40.0	29.0	41.0
27	35.585552467388744	38.0	34.0	40.0	28.0	41.0
28	35.42725671146275	38.0	34.0	40.0	27.0	41.0
29	35.66961985867927	38.0	34.0	40.0	29.0	41.0
30	35.246176008547934	37.0	34.0	40.0	27.0	41.0
31	35.437346960841055	38.0	34.0	40.0	28.0	41.0
32	35.269819564844084	38.0	34.0	40.0	27.0	41.0
33	35.24904439964129	38.0	34.0	40.0	27.0	41.0
34	35.14847580947777	38.0	34.0	40.0	26.0	41.0
35	35.24575942403216	38.0	34.0	40.0	27.0	41.0
36	34.93816104966578	38.0	33.0	40.0	25.0	41.0
37	34.963852548161626	38.0	34.0	40.0	26.0	41.0
38	34.8312005902144	37.0	33.0	40.0	25.0	41.0
39	34.7035349263186	37.0	33.0	40.0	25.0	41.0
40	34.70177318722135	37.0	33.0	40.0	25.0	41.0
41	34.63036551316216	37.0	33.0	40.0	25.0	41.0
42	34.80842200329452	37.0	33.0	40.0	26.0	41.0
43	34.63874172396029	37.0	33.0	40.0	25.0	41.0
44	34.7774580076448	37.0	33.0	40.0	26.0	41.0
45	34.73043483791364	37.0	34.0	40.0	26.0	41.0
46	34.61028677550865	37.0	34.0	40.0	25.0	41.0
47	34.44674078267008	37.0	33.0	40.0	25.0	41.0
48	34.54142948909566	37.0	33.0	40.0	26.0	41.0
49	34.62742716131043	37.0	33.0	40.0	26.0	41.0
50	34.316000025440275	36.0	33.0	40.0	25.0	41.0
51	33.343415102619716	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	9.0
13	6.0
14	6.0
15	18.0
16	24.0
17	76.0
18	131.0
19	340.0
20	905.0
21	1596.0
22	2417.0
23	3077.0
24	3671.0
25	4107.0
26	4347.0
27	4849.0
28	6009.0
29	7623.0
30	9865.0
31	12731.0
32	16205.0
33	21180.0
34	29600.0
35	29962.0
36	34820.0
37	43369.0
38	51451.0
39	26063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.554407209774155	4.000165361792521	49.40469754692141	16.040729881511915
2	18.966679598806852	13.283639994657543	49.21802952344003	18.531650883095573
3	20.62252354815526	13.010157030102206	47.48204870540796	18.885270716334563
4	18.86523649916365	4.832062379556194	51.44596167422455	24.856739447055606
5	24.94800643638977	5.6871736489623546	45.08430271384142	24.280517200806457
6	20.6133014481877	14.190903829397511	47.938701655525946	17.257093066888846
7	77.72226850939064	2.2495563851912155	15.881410154486076	4.146764950932067
8	78.33474314861573	10.574250624876774	6.328586601878765	4.762419624628731
9	73.45021020027858	5.165329992177115	7.431422556620514	13.9530372509238
10	44.2959721683383	27.03442705318926	14.704479396556657	13.965121381915779
11	36.750386374188295	22.882892050549827	22.301899752593318	18.06482182266856
12	29.82522530544231	20.299750049290534	25.57669925141989	24.29832539384727
13	27.54704861000693	23.502044762165223	29.356806227779508	19.594100400048337
14	19.89652167829499	31.01742022883528	26.669995102746913	22.41606299012281
15	16.942269654203052	23.325552848992885	36.04887076975915	23.68330672704492
16	18.681748510153852	21.702781258148836	32.56864104406892	27.046829187628397
17	19.6487969929594	25.887070615845474	29.499589775553165	24.964542615641953
18	20.37893290763272	22.417971010805758	29.6843497783516	27.51874630320993
19	22.755054664792564	25.619947720233288	25.701356602705573	25.923641012268572
20	29.86529373978414	23.54115918616558	29.08364126667133	17.509905807378953
21	25.112414218570127	29.93907053952465	25.13403845297683	19.814476788928392
22	22.563616589603832	22.30571579395921	30.53437299260324	24.59629462383372
23	25.469214086280694	26.910405708797885	25.74015302325877	21.880227181662647
24	22.80879724736216	25.76813732660862	28.370359534697354	23.05270589133186
25	20.677856147960643	31.010742156444977	25.33819666605186	22.97320502954252
26	20.169050632508856	26.652822916600417	29.726008229929214	23.452118220961516
27	24.53396594819088	27.796363312578308	25.341058697076274	22.328612042154536
28	18.075951943319065	30.505434678911918	30.430067861935623	20.988545515833394
29	21.76510993379168	22.79226106810998	32.0712836527148	23.371345345383542
30	21.69801120644148	30.49684858583867	27.159084404474942	20.646055803244906
31	23.48773460704314	25.854316260788266	23.752313475078072	26.90563565709052
32	28.478798710178015	26.95556219829423	23.31251470765943	21.25312438386832
33	27.344480414167688	24.549548117101587	24.05187272230031	24.054098746430412
34	22.14099000833169	24.976626746633933	27.55436268929155	25.328020555742825
35	27.09198567712474	22.674281789214596	25.723934847453744	24.50979768620692
36	22.76332275441866	28.874076994994624	25.631077840883794	22.73152240970292
37	23.0046873708111	25.788171543779537	26.939026019042046	24.26811506636732
38	22.842823616208	26.505269317119396	23.369437324700602	27.282469741972005
39	21.7679719648161	26.35421767971965	27.419211224249672	24.45859913121458
40	24.96835865700784	22.184238477145094	27.796363312578308	25.051039553268755
41	19.23602851854914	24.568628323931033	28.364953476095682	27.830389681424144
42	24.51679376204438	23.031081656925164	27.273565645451598	25.17855893557886
43	22.38012860059403	25.297492224815716	25.57351921694831	26.74885995764194
44	23.053659901673335	24.083037060121733	28.888387150116706	23.974915888088226
45	22.206498718446106	24.744484230209054	26.165005628661014	26.884011422683823
46	23.90940717797381	27.345434424509165	25.335016631580288	23.410141765936743
47	19.64307293091057	27.169260514783982	30.471726313513237	22.71594024079221
48	21.55300163453772	25.001749018959362	28.81206632279894	24.633183023703978
49	22.25483524241403	22.92614051936323	30.2542119556576	24.564812282565143
50	22.452951389993068	24.111339366918738	27.565174806494902	25.870534436593296
51	19.7219377858056	23.818140188639646	27.656441795829068	28.803480229725693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	70.0
1	172.5
2	275.0
3	440.5
4	606.0
5	478.5
6	351.0
7	373.0
8	395.0
9	422.0
10	449.0
11	488.0
12	527.0
13	517.5
14	508.0
15	559.0
16	610.0
17	574.0
18	538.0
19	637.0
20	736.0
21	894.0
22	1052.0
23	1184.0
24	1316.0
25	1514.0
26	2215.5
27	2719.0
28	3314.5
29	3910.0
30	4611.5
31	5313.0
32	6107.0
33	6901.0
34	7764.5
35	8628.0
36	9110.0
37	9592.0
38	10815.0
39	12038.0
40	12700.0
41	13362.0
42	15212.5
43	17063.0
44	17603.0
45	18143.0
46	18989.0
47	19835.0
48	27728.0
49	35621.0
50	32016.5
51	28412.0
52	26986.0
53	25560.0
54	22614.5
55	19669.0
56	18713.5
57	17758.0
58	16961.0
59	16164.0
60	14937.5
61	13711.0
62	11744.5
63	9778.0
64	8742.0
65	7706.0
66	6103.0
67	4500.0
68	3790.0
69	3080.0
70	2765.5
71	2451.0
72	1928.0
73	1405.0
74	1177.0
75	700.5
76	452.0
77	383.0
78	314.0
79	235.0
80	156.0
81	110.0
82	64.0
83	52.5
84	41.0
85	29.5
86	18.0
87	10.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.857888331587937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6979999987927	23.797205425544934
2	8.321999999865495	5.302426933823796
3	3.0119999999513185	2.878678789595759
4	1.613000001583861	2.055470957172392
5	1.1219999999881514	1.7872275353832094
6	0.884999999985724	1.6916538703800312
7	0.819999999986747	1.8286427902035927
8	0.7099999999885246	1.8095280572049481
9	0.6599999999893328	1.892358566865738
>10	8.005999999870603	47.23250523964887
>50	0.1069999999982706	2.2909007498874616
>100	0.03399999999945048	2.231326498708355
>500	0.006999999999886863	1.5164354845590764
>1k	0.00399999999993535	3.685639101021839
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	4198	1.3349784711666275	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	3385	1.0764416686276879	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	2368	0.7530321628686455	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT	1618	0.5145295775006201	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA	910	0.2893831369132041	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	806	0.256310778408838	TruSeq Adapter, Index 22 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	674	0.2143343233840655	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC	664	0.21115428891249183	No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	583	0.18539600969274506	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT	564	0.1793539441967551	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	559	0.17776392696096827	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCGAATGC	440	0.13992151674924155	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	393	0.12497535473284531	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	373	0.11861528578969796	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	370	0.11766127544822587	No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	351	0.11161920995223588	TruSeq Adapter, Index 22 (95% over 24bp)
TTTTCCGGGACATCTTCTATGAAAAAACTTTCTACCCCTAACACTAGCATT	339	0.10780316858634749	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGT	338	0.10748516513919011	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20606623375797395	0.0
2	0.0	0.0	0.0	0.880869548625907	0.0
3	0.0	0.0	0.0	1.108560016790582	0.0
4	0.0	0.0	0.0	1.678422194096584	0.0
5	0.0	0.0	0.0	3.1018056235729596	0.0
6	0.0	0.0	0.0	3.410586970762763	0.0
7	0.0	0.0	0.0	3.915576444848662	0.0
8	0.0	0.0	0.0	4.598011842448372	0.0
9	0.0	0.0	0.0	4.801852052076245	0.0
10	0.0	0.0	0.0	6.470098135863792	0.0
11	0.0	0.0	0.0	7.280370919220764	0.0
12	0.0	0.0	0.0	9.706101214137162	0.0
13	0.0	0.0	0.0	10.068307140449402	0.0
14	0.0	0.0	0.0	10.245435060516057	0.0
15	0.0	0.0	0.0	10.76791472419561	0.0
16	0.0	0.0	0.0	11.227111701890848	0.0
17	0.0	0.0	0.0	11.761675496562383	0.0
18	0.0	0.0	0.0	12.279385108534576	0.0
19	0.0	0.0	0.0	13.319574384186325	0.0
20	0.0	0.0	0.0	13.774955320515675	0.0
21	0.0	0.0	0.0	14.348951542634723	0.0
22	0.0	0.0	0.0	14.942663978477526	0.0
23	0.0	0.0	0.0	15.516342197149417	0.0
24	0.0	0.0	0.0	15.973313150714553	0.0
25	0.0	0.0	0.0	16.36954544587263	0.0
26	0.0	0.0	0.0	16.734931406656447	0.0
27	0.0	0.0	0.0	17.10158938122889	0.0
28	0.0	0.0	0.0	17.479695479899004	0.0
29	0.0	0.0	0.0	17.853667533756067	0.0
30	0.0	0.0	0.0	18.30141638735364	0.0
31	0.0	0.0	0.0	18.76156737539035	0.0
32	0.0	0.0	0.0	19.189282011817006	0.0
33	0.0	0.0	0.0	19.586150313869403	0.0
34	0.0	0.0	0.0	20.00050880551545	0.0
35	0.0	0.0	0.0	20.465111841812366	0.0
36	0.0	0.0	0.0	20.922718802271817	0.0
37	0.0	0.0	0.0	21.380007759284112	0.0
38	0.0	0.0	0.0	21.822668557727166	0.0
39	0.0	0.0	0.0	22.262467325145806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCAC	30	2.1604537E-6	45.000004	30
ACGGCTC	60	0.0	45.000004	21
CACCGCG	65	0.0	45.000004	38
TTGATAG	20	7.025463E-4	45.0	31
GTCTGCG	20	7.025463E-4	45.0	1
CCGATCG	35	1.2081728E-7	45.0	40
ACACCGA	20	7.025463E-4	45.0	37
CAGCATA	20	7.025463E-4	45.0	20
CCGTGCA	20	7.025463E-4	45.0	41
GTTAGGG	70	0.0	45.0	3
TACGGAG	45	3.8198777E-10	45.0	29
CGTTCGG	25	3.8843227E-5	45.0	2
TTATCGG	20	7.025463E-4	45.0	2
AGAACCC	20	7.025463E-4	45.0	10
ACGATTA	20	7.025463E-4	45.0	12
TTAAGGG	25	3.8843227E-5	45.0	3
TACGAAT	45	3.8198777E-10	45.0	12
TTGACGG	20	7.025463E-4	45.0	2
CGTACAA	20	7.025463E-4	45.0	10
ATACGGA	45	3.8198777E-10	45.0	28
>>END_MODULE