##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934005.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	288311
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.9028791825494	31.0	30.0	31.0	25.0	34.0
2	29.981193919066563	31.0	30.0	33.0	25.0	34.0
3	30.04843727780071	31.0	30.0	33.0	25.0	34.0
4	33.91511250004336	35.0	33.0	37.0	28.0	37.0
5	34.40270055599682	35.0	35.0	37.0	32.0	37.0
6	33.726215787812464	35.0	33.0	37.0	28.0	37.0
7	34.37400931632855	35.0	35.0	37.0	32.0	37.0
8	34.15822150386215	35.0	35.0	37.0	30.0	37.0
9	35.76300938916656	37.0	35.0	39.0	30.0	39.0
10	35.2159716417341	37.0	34.0	39.0	30.0	39.0
11	35.34395496529789	37.0	34.0	39.0	30.0	39.0
12	35.333507913329704	37.0	35.0	39.0	30.0	39.0
13	35.23168384140737	37.0	34.0	39.0	30.0	39.0
14	35.91498763488039	38.0	34.0	40.0	28.0	41.0
15	36.16337219183451	38.0	34.0	40.0	30.0	41.0
16	36.42493696043508	38.0	35.0	40.0	31.0	41.0
17	36.12022087259938	38.0	34.0	40.0	30.0	41.0
18	36.26785311694663	38.0	35.0	40.0	30.0	41.0
19	36.248103610337445	38.0	34.0	40.0	30.0	41.0
20	36.195407043088885	38.0	34.0	40.0	30.0	41.0
21	35.9674830304775	38.0	34.0	40.0	29.0	41.0
22	36.17372906340722	38.0	34.0	40.0	30.0	41.0
23	36.15220022822577	38.0	34.0	40.0	30.0	41.0
24	36.080107938996434	38.0	34.0	40.0	30.0	41.0
25	35.701218475881944	38.0	34.0	40.0	29.0	41.0
26	35.80233151007072	38.0	34.0	40.0	29.0	41.0
27	35.73776928386361	38.0	34.0	40.0	29.0	41.0
28	35.64138378348381	38.0	34.0	40.0	29.0	41.0
29	35.76055370762822	38.0	34.0	40.0	29.0	41.0
30	35.36464442910607	38.0	34.0	40.0	27.0	41.0
31	35.5127345123842	38.0	34.0	40.0	28.0	41.0
32	35.454682617035076	38.0	34.0	40.0	28.0	41.0
33	35.45318423507948	38.0	34.0	40.0	27.0	41.0
34	35.44241808325038	38.0	34.0	40.0	28.0	41.0
35	35.40700493564241	38.0	34.0	40.0	27.0	41.0
36	35.15556118219561	38.0	34.0	40.0	27.0	41.0
37	35.22651927952801	38.0	34.0	40.0	27.0	41.0
38	35.164846294452865	38.0	34.0	40.0	27.0	41.0
39	35.09517153351762	37.0	34.0	40.0	27.0	41.0
40	35.010266691177236	37.0	33.0	40.0	26.0	41.0
41	34.85096302256938	37.0	33.0	40.0	26.0	41.0
42	35.05141323085141	37.0	33.0	40.0	27.0	41.0
43	35.00864344405867	37.0	33.0	40.0	27.0	41.0
44	34.99658355040217	37.0	34.0	40.0	27.0	41.0
45	35.14177398711808	37.0	34.0	40.0	27.0	41.0
46	35.01101241367829	37.0	34.0	40.0	27.0	41.0
47	34.8913499658355	37.0	33.0	40.0	26.0	41.0
48	34.92593067902369	37.0	33.0	40.0	27.0	41.0
49	34.91397483966966	37.0	34.0	40.0	27.0	41.0
50	34.68688672995481	37.0	33.0	40.0	26.0	41.0
51	33.64226477657807	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	0.0
12	3.0
13	2.0
14	9.0
15	18.0
16	34.0
17	65.0
18	116.0
19	305.0
20	644.0
21	1030.0
22	1469.0
23	1894.0
24	2370.0
25	2953.0
26	3667.0
27	4556.0
28	5759.0
29	7312.0
30	9418.0
31	12048.0
32	15233.0
33	19628.0
34	26660.0
35	27586.0
36	33103.0
37	41454.0
38	48281.0
39	22689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.551883209450907	4.598853321586758	52.17109302107793	16.6781704478844
2	17.860227323966132	8.412790354859856	53.6226505405621	20.104331780611908
3	20.365854927491494	8.350357773376667	50.892959339047074	20.39082796008477
4	19.3221902736975	4.779560960213104	49.90617770393776	25.992071062151634
5	21.43588000457839	5.482274349573898	48.70296311968673	24.378882526160986
6	20.685995331430295	10.263222700486626	51.03655427645841	18.01422769162467
7	79.922028642681	2.666218077007121	13.003666179923762	4.408087100388122
8	80.9663869918248	6.913021008563669	6.928976001609373	5.191615998002157
9	75.97386155921903	5.039003021043249	8.9240438276722	10.063091592065513
10	42.712556926374646	24.966789335127693	17.161329259029312	15.159324479468353
11	33.11250698030946	25.08055537249706	23.566218423854796	18.240719223338687
12	27.680872391271926	21.683876092136618	28.9021230546181	21.733128461973354
13	25.604642209280946	24.592193846228554	30.342234600830352	19.460929343660144
14	20.094273198039616	28.669735112430672	28.767199309079434	22.468792380450278
15	17.263649323126764	25.201605211039467	35.90879293540656	21.625952530427213
16	20.682526854681228	24.376107744761732	30.82851504104942	24.112850359507615
17	20.356490040269016	25.707655968728215	30.124067413314094	23.811786577688675
18	20.811901037421396	24.56895505200981	30.75775811536847	23.86138579520032
19	22.234670199888313	26.586567976941566	28.38670740970688	22.79205441346324
20	26.16445435658022	26.369788180124935	29.86046318038507	17.605294282909774
21	24.873140462902906	28.85946079060459	27.18071804405659	19.086680702435913
22	22.64360360860321	24.52802702637083	29.8254315652195	23.002937799806457
23	22.475035638598598	27.235173129016932	28.394684906229735	21.895106326154743
24	22.206228690545974	25.34762808217515	28.95206911980466	23.49407410747422
25	19.710659669592907	28.9333393453597	27.07146102646101	24.284539958586386
26	19.65065502183406	27.426979893240283	28.3561848143151	24.566180270610555
27	21.622137206003238	29.388056647162266	27.31147961749638	21.67832652933811
28	18.68884641931803	28.02217050338003	30.395649142765972	22.89333393453597
29	19.68984880909851	26.05727842503408	28.74604160091013	25.506831164957283
30	21.6252588350774	29.17543902244451	28.192472711759176	21.006829430718913
31	23.568299509904236	26.72842867597837	25.411448054357965	24.291823759759428
32	24.139210782800518	29.06756939554856	24.73336084991554	22.059858971735384
33	24.956037057205588	26.221337375264902	25.45619140442092	23.366434163108586
34	22.043210283339864	26.207116620593734	27.315988637270173	24.433684458796233
35	21.44524489180087	27.407556423445516	25.465209443968494	25.681989240785125
36	23.214514881499493	28.233053889723248	25.481164437014197	23.071266791763062
37	20.97838792137657	29.553156140417812	27.28720028025292	22.181255657952697
38	23.388285566627705	27.763422137899695	24.470450312336332	24.377841983136268
39	22.665801859797234	25.841539171242168	27.886899910166452	23.60575905879415
40	22.680716309818216	25.27999278556836	28.497698665676996	23.541592238936428
41	20.602405041777804	25.787777781631643	28.27502245838695	25.334794718203607
42	21.84307917491875	25.992764757501448	27.061402443888717	25.102753623691083
43	23.568299509904236	25.325776678656037	27.01735278917558	24.08857102226415
44	21.36755101262179	25.22657824363274	28.338842430569766	25.067028313175705
45	21.748389759669244	25.213398031986294	25.993458452851257	27.0447537554932
46	23.030685613798987	27.060361900864	26.65593751192289	23.25301497341413
47	19.92813316175935	26.716289007356643	30.37830675902064	22.97727107186337
48	21.341190589328885	25.27201528904551	28.86882567782707	24.517968443798537
49	21.811169188827343	23.995962693064087	30.785505929361	23.40736218874757
50	22.52983757123384	24.172161311916646	28.299648643305318	24.998352473544195
51	20.361345907717705	24.405589797128798	26.974343677487155	28.258720617666338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	181.5
2	290.0
3	449.0
4	608.0
5	477.0
6	346.0
7	389.0
8	432.0
9	499.5
10	567.0
11	608.5
12	650.0
13	630.5
14	611.0
15	654.5
16	698.0
17	649.5
18	601.0
19	691.5
20	782.0
21	987.5
22	1193.0
23	1191.0
24	1189.0
25	1736.0
26	2928.5
27	3574.0
28	3829.0
29	4084.0
30	4769.5
31	5455.0
32	6527.0
33	7599.0
34	7971.5
35	8344.0
36	8968.0
37	9592.0
38	10353.5
39	11115.0
40	12355.5
41	13596.0
42	14955.0
43	16314.0
44	17393.5
45	18473.0
46	19123.5
47	19774.0
48	24702.0
49	29630.0
50	29198.5
51	28767.0
52	26939.0
53	25111.0
54	21846.0
55	18581.0
56	16364.5
57	14148.0
58	13291.5
59	12435.0
60	11253.0
61	10071.0
62	8831.0
63	7591.0
64	6181.0
65	4771.0
66	4032.5
67	3294.0
68	2760.0
69	2226.0
70	1832.0
71	1438.0
72	1146.5
73	855.0
74	698.5
75	431.0
76	320.0
77	246.5
78	173.0
79	113.5
80	54.0
81	42.5
82	31.0
83	16.0
84	1.0
85	7.0
86	13.0
87	13.5
88	14.0
89	8.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	288311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.60601403820128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86816285482999	25.94544829547013
2	9.911288675163329	7.058029674090617
3	3.440467275828952	3.6750397836341335
4	1.8191253511353145	2.5908721115908735
5	1.3463217386975443	2.3968575364000158
6	1.027480702478795	2.195069538986453
7	0.9795048380211988	2.441338410914821
8	0.8695604104298307	2.476926414466289
9	0.7826043689429518	2.5078879932246356
>10	6.845539364829681	41.103099126020844
>50	0.06796564123350167	1.6509283950714757
>100	0.03298332589272875	2.028517320954389
>500	0.003997978896088333	1.1181756881471387
>1k	0.004997473620110417	2.8118097110281806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGC	2400	0.832434419775867	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG	2027	0.703060237035701	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	1297	0.4498614343538748	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	1118	0.3877757005455914	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCGTAGT	1059	0.3673116877261013	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	917	0.31805931788936254	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	890	0.308694430666884	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	802	0.27817183527510225	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTA	533	0.18486981072522382	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	367	0.12729309669072633	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTC	366	0.12694624901581972	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	341	0.11827505714315445	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT	319	0.110644408295209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33678909233431953	0.0
2	0.0	0.0	0.0	1.4539854532085144	0.0
3	0.0	0.0	0.0	1.8150538827862968	0.0
4	0.0	0.0	0.0	2.5184609674969045	0.0
5	0.0	0.0	0.0	3.990135652125656	0.0
6	0.0	0.0	0.0	4.516997270308798	0.0
7	0.0	0.0	0.0	5.149647429338457	0.0
8	0.0	0.0	0.0	6.071221701565324	0.0
9	0.0	0.0	0.0	6.396911668302631	0.0
10	0.0	0.0	0.0	7.856099836634745	0.0
11	0.0	0.0	0.0	9.158859703583976	0.0
12	0.0	0.0	0.0	11.32943245314955	0.0
13	0.0	0.0	0.0	11.783456059602305	0.0
14	0.0	0.0	0.0	11.96000152612977	0.0
15	0.0	0.0	0.0	12.420962086080657	0.0
16	0.0	0.0	0.0	13.031067146241385	0.0
17	0.0	0.0	0.0	13.844771791572295	0.0
18	0.0	0.0	0.0	14.737210859107005	0.0
19	0.0	0.0	0.0	15.744456507035805	0.0
20	0.0	0.0	0.0	16.429133817301455	0.0
21	0.0	0.0	0.0	17.283072792921534	0.0
22	0.0	0.0	0.0	18.174124469756617	0.0
23	0.0	0.0	0.0	19.002049869758697	0.0
24	0.0	0.0	0.0	19.622560360166627	0.0
25	0.0	0.0	0.0	20.226769009853943	0.0
26	0.0	0.0	0.0	20.741144111740446	0.0
27	0.0	0.0	0.0	21.269739968298122	0.0
28	0.0	0.0	0.0	21.780993441110468	0.0
29	0.0	0.0	0.0	22.35190471400675	0.0
30	0.0	0.0	0.0	22.99079813118473	0.0
31	0.0	0.0	0.0	23.59743471459639	0.0
32	0.0	0.0	0.0	24.185341523563096	0.0
33	0.0	0.0	0.0	24.74272573713802	0.0
34	0.0	0.0	0.0	25.302537884437292	0.0
35	0.0	0.0	0.0	25.9116024015733	0.0
36	0.0	0.0	0.0	26.52066691870931	0.0
37	0.0	0.0	0.0	27.12973143584532	0.0
38	0.0	0.0	0.0	27.718678787836744	0.0
39	0.0	0.0	0.0	28.344738841043178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	50	2.1827873E-11	45.000004	43
GCCGGGT	25	3.8835118E-5	45.000004	4
CGACCAA	25	3.8835118E-5	45.000004	34
ATCCGGC	25	3.8835118E-5	45.000004	6
TTAGTCA	25	3.8835118E-5	45.000004	32
CGGTCGA	25	3.8835118E-5	45.000004	41
TTGTTCG	35	1.2076998E-7	45.000004	1
GCGTCCC	25	3.8835118E-5	45.000004	7
TTCGATA	25	3.8835118E-5	45.000004	15
TGCGTTA	25	3.8835118E-5	45.000004	22
CCTTACA	20	7.0244865E-4	45.0	7
CGCGGAA	20	7.0244865E-4	45.0	41
CGTGAGT	20	7.0244865E-4	45.0	29
ATGTCGT	20	7.0244865E-4	45.0	38
TCGATAT	20	7.0244865E-4	45.0	16
GTAATAG	20	7.0244865E-4	45.0	13
TAGCACA	20	7.0244865E-4	45.0	44
ATGCGTT	20	7.0244865E-4	45.0	21
GTGAGTC	20	7.0244865E-4	45.0	30
TATACGA	20	7.0244865E-4	45.0	16
>>END_MODULE