##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934004.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	217225
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.845538036597997	31.0	30.0	31.0	25.0	34.0
2	30.08558407181494	31.0	30.0	33.0	26.0	34.0
3	30.178561399470595	31.0	30.0	33.0	25.0	34.0
4	33.91880308435954	35.0	33.0	37.0	28.0	37.0
5	34.26435262976177	35.0	35.0	37.0	32.0	37.0
6	33.770242835769366	35.0	35.0	37.0	29.0	37.0
7	34.22896075497756	35.0	35.0	37.0	31.0	37.0
8	34.267455403383586	35.0	35.0	37.0	31.0	37.0
9	35.533782943952126	37.0	35.0	39.0	30.0	39.0
10	35.07633099320981	37.0	34.0	39.0	30.0	39.0
11	35.321841408677635	37.0	34.0	39.0	30.0	39.0
12	35.24702957762688	37.0	34.0	39.0	30.0	39.0
13	35.133538957302335	37.0	34.0	39.0	29.0	39.0
14	35.75066866152607	38.0	34.0	40.0	27.0	41.0
15	36.09385199677754	38.0	34.0	40.0	30.0	41.0
16	36.26016342502014	38.0	34.0	40.0	30.0	41.0
17	36.03297502589481	38.0	34.0	40.0	30.0	41.0
18	36.12485211186558	38.0	34.0	40.0	30.0	41.0
19	36.12393140752676	38.0	34.0	40.0	30.0	41.0
20	36.181268270226724	38.0	34.0	40.0	30.0	41.0
21	35.834365289446424	38.0	34.0	40.0	29.0	41.0
22	36.01499827367937	38.0	34.0	40.0	30.0	41.0
23	36.14085395327425	38.0	34.0	40.0	30.0	41.0
24	36.054943031419036	38.0	34.0	40.0	30.0	41.0
25	35.533741512256874	38.0	34.0	40.0	28.0	41.0
26	35.65608470479917	38.0	34.0	40.0	29.0	41.0
27	35.659564967199906	38.0	34.0	40.0	29.0	41.0
28	35.430484520658304	38.0	34.0	40.0	27.0	41.0
29	35.46996892622857	38.0	34.0	40.0	28.0	41.0
30	35.052908274830244	37.0	34.0	40.0	27.0	41.0
31	35.157463459546555	37.0	34.0	40.0	27.0	41.0
32	35.29026585337783	37.0	34.0	40.0	27.0	41.0
33	35.34008056162965	38.0	34.0	40.0	28.0	41.0
34	35.28923926804005	38.0	34.0	40.0	27.0	41.0
35	35.32371964552883	38.0	34.0	40.0	27.0	41.0
36	35.09883761077224	37.0	33.0	40.0	27.0	41.0
37	35.10473011854069	37.0	33.0	40.0	27.0	41.0
38	34.95718724824491	37.0	33.0	40.0	27.0	41.0
39	34.91691564046496	37.0	33.0	40.0	26.0	41.0
40	34.86614339970077	37.0	33.0	40.0	26.0	41.0
41	34.73519622511221	37.0	33.0	40.0	26.0	41.0
42	34.97024283576936	37.0	33.0	40.0	27.0	41.0
43	34.911690643342155	37.0	33.0	40.0	27.0	41.0
44	34.82577051444355	37.0	33.0	40.0	26.0	41.0
45	34.925929335941994	37.0	33.0	40.0	27.0	41.0
46	34.78843595350443	37.0	33.0	40.0	26.0	41.0
47	34.7770790654851	37.0	33.0	40.0	26.0	41.0
48	34.770040280814825	37.0	33.0	40.0	26.0	41.0
49	34.718319714581654	37.0	33.0	40.0	26.0	41.0
50	34.5119668546438	36.0	33.0	40.0	26.0	41.0
51	33.424076418460125	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	14.0
10	22.0
11	28.0
12	29.0
13	16.0
14	15.0
15	21.0
16	26.0
17	57.0
18	102.0
19	236.0
20	389.0
21	614.0
22	911.0
23	1321.0
24	1825.0
25	2286.0
26	3011.0
27	3650.0
28	4677.0
29	5838.0
30	7487.0
31	9445.0
32	11963.0
33	15468.0
34	21331.0
35	21399.0
36	24584.0
37	30169.0
38	34747.0
39	15542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.252388076878812	4.800552422603292	53.80918402577972	18.137875474738173
2	19.260674415928186	6.037518701806882	53.04361836805156	21.65818851421337
3	20.319944757739673	5.748417539417654	51.755552998043505	22.17608470479917
4	21.373230521348834	4.886177926113477	47.125330878121765	26.61526067441593
5	19.912533087812175	6.462884106341352	48.52572217746577	25.09886062838071
6	21.4915410288871	7.981125561054206	51.70215214639199	18.825181263666703
7	80.86914489584532	2.6553113131545634	11.637242490505237	4.838301300494878
8	81.7171135918978	4.400506387386351	7.799286454137415	6.083093566578433
9	75.01622741397169	6.1180803314535614	10.99182874899298	7.87386350558177
10	39.525837265508116	28.848889400391297	16.989296812061227	14.635976522039359
11	31.71458165496605	25.59465991483485	24.372885257221775	18.31787317297733
12	27.970077108988377	21.292668891702153	30.731729773276555	20.005524226032914
13	25.385660029922892	25.433536655541488	30.26217056047877	18.91863275405685
14	18.528254114397512	29.64621935780872	29.868109103464153	21.957417424329613
15	16.278052710323397	26.25664633444585	36.98008976867303	20.485211186557716
16	18.47715502359305	24.317642996892623	33.49798595925883	23.707216020255494
17	18.662676947865116	25.378294395212336	28.775233053285763	27.183795603636785
18	22.01726320635286	22.76303372079641	30.816894924617333	24.402808148233397
19	23.433766831626194	24.78259868799632	27.852226953619518	23.93140752675797
20	26.0527103233974	24.76556565772816	28.45390723903786	20.727816779836576
21	25.102543445735986	26.309586833927955	28.154678328921623	20.433191391414432
22	21.710208309356656	25.895269881459313	27.666705029347447	24.727816779836576
23	20.99390033375532	25.9330187593509	27.33248935435608	25.74059155253769
24	22.564621935780874	23.50558177005409	29.309241569800896	24.620554724364137
25	19.776268845666937	26.166877661410982	27.423639083899182	26.633214409022905
26	19.928185061572105	27.016687766141096	26.936586488663828	26.11854068362297
27	21.618137875474737	26.46288410634135	28.291402923236276	23.627575094947634
28	18.35055817700541	25.581309701921974	33.0169179422258	23.05121417884682
29	19.30210611117505	24.199332489354354	31.700771089883762	24.797790309586834
30	21.85660029922891	24.700195649672	29.34146622165957	24.10173782943952
31	21.31476579583381	27.164000460352167	28.028081482333985	23.493152261480034
32	22.206467947980205	27.90838991828749	26.84083323742663	23.044308896305672
33	21.62918632754057	25.385660029922892	27.36563471055357	25.619518931982967
34	20.150995511566347	24.91518011278628	29.07998618943492	25.853838186212453
35	19.83841638853723	25.392104960294624	28.177695937392105	26.59178271377604
36	21.803659799746804	25.453792150995515	27.493612613649443	25.24893543560824
37	20.39129934399816	26.768557946829326	30.050868914719757	22.789273794452754
38	19.970997813327195	26.239152952008286	28.170330302681553	25.619518931982967
39	21.022902520428126	24.095753251237195	29.462538842214293	25.41880538612038
40	21.22821958798481	23.69340545517321	29.584532167107835	25.493842789734146
41	18.184371043848543	24.69651283231672	28.377488778915872	28.741627344918864
42	21.001265968465876	24.487052595235355	27.89365864886638	26.618022787432388
43	21.977672919783632	23.822304062607895	27.940154217976755	26.259868799631718
44	19.4052249971228	23.39279548854874	29.109448728277133	28.092530786051327
45	20.272988836459895	23.061341926573828	27.351824145471287	29.313845091494994
46	20.807918057313845	25.05282541143975	29.096558867533663	25.042697663712744
47	18.112095753251236	24.95246863850846	31.870180688226498	25.065254920013814
48	18.872597537115894	23.11704453907239	30.575670387846703	27.434687535965015
49	19.76890321095638	22.13465300955231	32.9883761077224	25.108067671768904
50	19.89642076188284	22.512141788468178	31.1414432040511	26.44999424559788
51	18.927839797445046	22.88686845436759	27.74772701116354	30.437564737023827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	183.5
2	250.0
3	411.0
4	572.0
5	444.0
6	316.0
7	326.0
8	336.0
9	348.5
10	361.0
11	407.5
12	454.0
13	506.5
14	559.0
15	461.0
16	363.0
17	468.5
18	574.0
19	624.5
20	675.0
21	775.0
22	875.0
23	860.0
24	845.0
25	1027.0
26	1710.5
27	2212.0
28	2528.5
29	2845.0
30	2911.5
31	2978.0
32	3499.5
33	4021.0
34	4583.0
35	5145.0
36	5344.0
37	5543.0
38	6379.5
39	7216.0
40	8651.0
41	10086.0
42	12191.0
43	14296.0
44	14936.5
45	15577.0
46	19194.5
47	22812.0
48	23496.0
49	24180.0
50	23244.0
51	22308.0
52	19127.0
53	15946.0
54	14084.5
55	12223.0
56	10928.0
57	9633.0
58	9382.0
59	9131.0
60	7899.5
61	6668.0
62	6370.5
63	6073.0
64	4930.0
65	3787.0
66	3125.0
67	2463.0
68	2034.0
69	1605.0
70	1413.0
71	1221.0
72	901.0
73	581.0
74	544.0
75	391.0
76	275.0
77	218.0
78	161.0
79	139.5
80	118.0
81	99.0
82	80.0
83	46.5
84	13.0
85	9.0
86	5.0
87	7.0
88	9.0
89	5.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	217225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.53297272413396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10507647971625	31.608700656001844
2	9.489026823320772	7.882149844631142
3	3.327421857681224	4.145931637702843
4	1.7679006872090446	2.9370468408332373
5	1.1471957437375304	2.3823224766946716
6	0.8689869208601196	2.1654966049027506
7	0.7714475726003103	2.242835769363563
8	0.7071602748836179	2.34963747266659
9	0.6572821990689426	2.4568995281390267
>10	5.026601640434493	34.47715502359305
>50	0.09199733983595655	2.5176660145010934
>100	0.032143648858346266	2.295776268845667
>500	0.0066504101086233645	1.9606398895154795
>1k	0.0011084016847705607	0.5777419726090459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1255	0.5777419726090459	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	913	0.42030153067096326	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	851	0.39175969616756817	TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	766	0.3526297617677523	TruSeq Adapter, Index 23 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	651	0.2996892622856485	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	560	0.25779721486937507	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	518	0.23846242375417195	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	393	0.18091840257797215	No Hit
TTTATGGGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	286	0.13166072045114513	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCTACT	268	0.12337438140177234	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.11785015536885718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4557486477155024	0.0
2	0.0	0.0	0.0	1.9362412245367706	0.0
3	0.0	0.0	0.0	2.515824605823455	0.0
4	0.0	0.0	0.0	3.417654505696858	0.0
5	0.0	0.0	0.0	5.048682241915065	0.0
6	0.0	0.0	0.0	5.9960870065600185	0.0
7	0.0	0.0	0.0	6.741397168834158	0.0
8	0.0	0.0	0.0	7.903326044423984	0.0
9	0.0	0.0	0.0	8.33145356197491	0.0
10	0.0	0.0	0.0	9.453792150995511	0.0
11	0.0	0.0	0.0	11.147888134422834	0.0
12	0.0	0.0	0.0	12.952468638508458	0.0
13	0.0	0.0	0.0	13.719875704914259	0.0
14	0.0	0.0	0.0	14.036597997468062	0.0
15	0.0	0.0	0.0	14.421452411094487	0.0
16	0.0	0.0	0.0	15.143744964898147	0.0
17	0.0	0.0	0.0	16.293244331913915	0.0
18	0.0	0.0	0.0	17.587754632293706	0.0
19	0.0	0.0	0.0	18.476234319254228	0.0
20	0.0	0.0	0.0	19.321440902290252	0.0
21	0.0	0.0	0.0	20.37518701806882	0.0
22	0.0	0.0	0.0	21.35021291287835	0.0
23	0.0	0.0	0.0	22.297157325353897	0.0
24	0.0	0.0	0.0	23.087582000230174	0.0
25	0.0	0.0	0.0	23.76153757624583	0.0
26	0.0	0.0	0.0	24.407872022096903	0.0
27	0.0	0.0	0.0	25.062032454827943	0.0
28	0.0	0.0	0.0	25.676602600989757	0.0
29	0.0	0.0	0.0	26.359304868224193	0.0
30	0.0	0.0	0.0	27.11474277822534	0.0
31	0.0	0.0	0.0	27.80757279318679	0.0
32	0.0	0.0	0.0	28.48290942571067	0.0
33	0.0	0.0	0.0	29.175739440672114	0.0
34	0.0	0.0	0.0	29.851996777534815	0.0
35	0.0	0.0	0.0	30.527793762228104	0.0
36	0.0	0.0	0.0	31.178271377603867	0.0
37	0.0	0.0	0.0	31.81862124525262	0.0
38	0.0	0.0	0.0	32.46955921279779	0.0
39	4.6035216940959836E-4	0.0	0.0	33.210726205547246	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	20	7.020644E-4	45.0	1
GTCATAC	20	7.020644E-4	45.0	39
ACACGTG	25	3.88033E-5	45.0	42
ACCGGCG	25	3.88033E-5	45.0	20
ACACGCG	40	6.7702786E-9	45.0	36
ACTGCAT	20	7.020644E-4	45.0	38
GTACACG	25	3.88033E-5	45.0	16
ACGTTGC	20	7.020644E-4	45.0	15
CCCTAGC	35	1.2058445E-7	45.0	21
TGGGTAC	50	2.1827873E-11	45.0	6
GGCGTAC	20	7.020644E-4	45.0	8
GACCGAT	80	0.0	45.0	9
CACGCCA	20	7.020644E-4	45.0	27
TTCGTCG	20	7.020644E-4	45.0	1
TTCCGCG	20	7.020644E-4	45.0	1
CATTCTA	25	3.88033E-5	45.0	45
TATGCGG	20	7.020644E-4	45.0	2
ACTTACT	90	0.0	45.0	38
TCTAGCG	35	1.2058445E-7	45.0	1
ACGGGCA	25	3.88033E-5	45.0	5
>>END_MODULE