##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934003.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197964
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.860691842961348	31.0	30.0	31.0	25.0	34.0
2	29.9328968903437	31.0	30.0	33.0	25.0	34.0
3	29.94559111757693	31.0	30.0	33.0	25.0	34.0
4	33.851634640641734	35.0	33.0	37.0	28.0	37.0
5	34.354433129255824	35.0	35.0	37.0	32.0	37.0
6	33.736310642339014	35.0	35.0	37.0	29.0	37.0
7	34.3753712796266	35.0	35.0	37.0	32.0	37.0
8	34.255748519932915	35.0	35.0	37.0	31.0	37.0
9	35.72621284678022	37.0	35.0	39.0	30.0	39.0
10	35.21375603645107	37.0	34.0	39.0	30.0	39.0
11	35.38975773372937	37.0	35.0	39.0	30.0	39.0
12	35.278616314077304	37.0	34.0	39.0	30.0	39.0
13	35.15320462306278	37.0	34.0	39.0	29.0	39.0
14	35.76005233274737	38.0	34.0	40.0	27.0	41.0
15	35.986123739669836	38.0	34.0	40.0	30.0	41.0
16	36.243044189852704	38.0	34.0	40.0	30.0	41.0
17	36.06026853367279	38.0	34.0	40.0	30.0	41.0
18	36.11489462730597	38.0	34.0	40.0	30.0	41.0
19	36.10428663797458	38.0	34.0	40.0	30.0	41.0
20	36.07340728617324	38.0	34.0	40.0	30.0	41.0
21	35.74472126245176	38.0	34.0	40.0	29.0	41.0
22	35.98043078539533	38.0	34.0	40.0	30.0	41.0
23	36.05366127174638	38.0	34.0	40.0	30.0	41.0
24	35.9591238811097	38.0	34.0	40.0	30.0	41.0
25	35.553242003596615	38.0	34.0	40.0	29.0	41.0
26	35.63734820472409	38.0	34.0	40.0	29.0	41.0
27	35.64249055383807	38.0	34.0	40.0	29.0	41.0
28	35.533071669596495	38.0	34.0	40.0	29.0	41.0
29	35.64274817643612	38.0	34.0	40.0	29.0	41.0
30	35.300140429573055	37.0	34.0	40.0	27.0	41.0
31	35.45789133377786	37.0	34.0	40.0	29.0	41.0
32	35.43023984158736	38.0	34.0	40.0	28.0	41.0
33	35.407250813279184	38.0	34.0	40.0	28.0	41.0
34	35.25293992847185	37.0	34.0	40.0	27.0	41.0
35	35.30465640217413	38.0	34.0	40.0	28.0	41.0
36	35.12758380311572	37.0	34.0	40.0	27.0	41.0
37	35.21520074356954	37.0	34.0	40.0	28.0	41.0
38	35.06159705805096	37.0	33.0	40.0	27.0	41.0
39	35.028692085429675	37.0	33.0	40.0	27.0	41.0
40	34.975056070800754	37.0	33.0	40.0	27.0	41.0
41	34.83315148208765	37.0	33.0	40.0	26.0	41.0
42	35.0202966195874	37.0	33.0	40.0	27.0	41.0
43	34.99117011173749	37.0	33.0	40.0	27.0	41.0
44	34.91033218160878	37.0	33.0	40.0	27.0	41.0
45	35.028378897173226	37.0	34.0	40.0	27.0	41.0
46	34.93649350386939	37.0	33.0	40.0	27.0	41.0
47	34.797624820674464	37.0	33.0	40.0	26.0	41.0
48	34.803413751995315	37.0	33.0	40.0	27.0	41.0
49	34.85438261502091	36.0	33.0	40.0	27.0	41.0
50	34.61469762178982	36.0	33.0	40.0	26.0	41.0
51	33.5094057505405	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	4.0
13	8.0
14	5.0
15	10.0
16	21.0
17	52.0
18	105.0
19	202.0
20	355.0
21	554.0
22	838.0
23	1099.0
24	1533.0
25	1974.0
26	2499.0
27	3193.0
28	4064.0
29	5053.0
30	6791.0
31	8583.0
32	11129.0
33	14500.0
34	19628.0
35	20196.0
36	23198.0
37	27686.0
38	31017.0
39	13656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.48475480390374	4.236628882018953	53.682992867390034	17.595623446687274
2	19.405043341213553	8.108039845628499	51.88923238568629	20.59768442747166
3	20.52140793275545	8.140874098320907	50.80772261623325	20.52999535269039
4	19.176718999414035	4.793800893091673	49.94392919924835	26.085550908245942
5	20.63809581540078	5.176193651370956	49.632256369845024	24.553454163383243
6	20.43906972984987	9.263805540401284	51.857913560041226	18.439211169707622
7	78.7724030631832	2.2983976884686106	13.881311753652179	5.047887494696005
8	79.13762098159262	5.890464933018125	8.246448849285729	6.725465236103534
9	73.73663898486593	5.45200137398719	10.61607160897941	10.195288032167465
10	41.85356933583884	23.962437614919885	18.798367379927665	15.385625669313614
11	31.863874239760765	22.987007738780786	24.805520195591118	20.34359782586733
12	26.285082136145967	21.09979592249096	29.56648683599038	23.048635105372696
13	24.806530480289346	25.82843345254693	31.203148047119676	18.16188802004405
14	19.8258269180255	30.418156836596555	28.95576973591158	20.800246509466366
15	16.136267200096988	23.692186458143905	39.13489321290739	21.03665312885171
16	18.36495524438787	24.20288537309814	32.752419631852256	24.679739750661735
17	19.638924248853325	23.047624820674468	29.573053686528866	27.74039724394334
18	19.97939019215615	22.33234325432907	32.78070760340264	24.90755895011214
19	23.511850639510214	24.696914590531613	28.639550625366226	23.151684144591947
20	26.41490371986826	24.15489684993231	30.62526519973329	18.804934230466145
21	23.604796831747183	27.18120466347417	29.63114505667697	19.582853448101677
22	22.24848962437615	24.09529005273686	29.389687013800486	24.266533309086498
23	22.011072720292578	26.098179466973793	27.42922955688913	24.461518255844496
24	21.08666222141399	23.709361298013782	29.95494130245903	25.249035178113193
25	20.08395465842274	27.52066032207876	27.78788062476006	24.607504394738438
26	18.914044977874763	27.92982562486109	28.056616354488696	25.099513042775456
27	19.76773554787739	28.710775696591302	27.1463498413853	24.375138914146007
28	16.096866096866098	26.15475541007456	34.05316118082076	23.69521731223859
29	18.117435493322017	24.584267846679193	32.64886545028389	24.649431209714898
30	19.38534278959811	25.322280818734722	31.62595219332808	23.666424198339094
31	22.79404336141925	25.81883574791376	27.307995393101773	24.079125497565215
32	23.99224101351761	26.88670667394072	26.565436139904225	22.55561617263745
33	23.330504536178296	24.953021761532398	26.885696389242487	24.830777313046816
34	20.29156816390859	24.699945444626294	30.438362530561115	24.570123860904005
35	19.204501828615303	25.080822775858238	28.113192297589464	27.601483097936995
36	23.876058273221393	25.562223434563858	28.3096926713948	22.252025620819946
37	21.208906669899577	24.45040512416399	31.13293326059284	23.2077549453436
38	19.65811965811966	26.14212685134671	29.573053686528866	24.62669980400477
39	21.780727809096604	23.97304560425128	32.353357176052214	21.892869410599907
40	22.038350407144733	22.81879533652583	30.789941605544442	24.35291265078499
41	21.76658382332141	22.979935745893194	27.567133418197248	27.686347012588147
42	22.868804429088115	22.889010123052675	27.249904022953668	26.99228142490554
43	22.880422703117738	22.679376048170376	28.43446283162595	26.005738417085933
44	19.85613545897234	23.13450930472207	32.008850093956475	25.000505142349116
45	20.737608858176234	23.530035764078317	28.52942959325938	27.20292578448607
46	22.867289002040774	24.592855266614134	29.689741569174195	22.8501141621709
47	18.26645248631064	24.570123860904005	32.78121274575175	24.382210907033603
48	20.94522236366208	23.036006546644845	30.758117637550264	25.26065345214281
49	22.192418823624497	20.1329534662868	33.52074114485462	24.153886565234085
50	21.8635711543513	22.074215513931826	30.582328100058596	25.479885231658283
51	20.562829605382795	21.734254712978117	27.914166212038555	29.788749469600535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	227.5
2	303.0
3	465.5
4	628.0
5	486.5
6	345.0
7	350.5
8	356.0
9	371.0
10	386.0
11	424.0
12	462.0
13	480.5
14	499.0
15	521.5
16	544.0
17	521.5
18	499.0
19	534.0
20	569.0
21	592.0
22	615.0
23	790.0
24	965.0
25	1050.5
26	1434.0
27	1732.0
28	1855.5
29	1979.0
30	2411.5
31	2844.0
32	3433.0
33	4022.0
34	4273.5
35	4525.0
36	5035.0
37	5545.0
38	6020.0
39	6495.0
40	7790.0
41	9085.0
42	10465.0
43	11845.0
44	13310.5
45	14776.0
46	19209.5
47	23643.0
48	23358.0
49	23073.0
50	21731.5
51	20390.0
52	17273.5
53	14157.0
54	12576.0
55	10995.0
56	9669.5
57	8344.0
58	7502.0
59	6660.0
60	6232.5
61	5805.0
62	5290.0
63	4775.0
64	4234.0
65	3693.0
66	3040.5
67	2388.0
68	1891.0
69	1394.0
70	1162.5
71	931.0
72	788.5
73	646.0
74	512.5
75	277.5
76	176.0
77	157.0
78	138.0
79	95.5
80	53.0
81	29.0
82	5.0
83	4.5
84	4.0
85	5.0
86	6.0
87	3.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.55585864096503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05293575467704	30.438362530561115
2	9.887153426500262	8.01963993453355
3	3.58593029918043	4.362914469297448
4	1.8384276212540167	2.98236042916894
5	1.3152978103280772	2.667151603321816
6	0.9839822634083152	2.3943747348002664
7	0.8581819993523155	2.436301549776727
8	0.7348728296340583	2.384271887817987
9	0.6613855466706524	2.414075286415712
>10	4.954786637769972	32.94841486330848
>50	0.08718830182098995	2.4352912650784995
>100	0.02989313205291084	2.30850053545089
>500	0.004982188675485141	1.4750156594128225
>1k	0.004982188675485141	2.7333252510557475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	1638	0.8274231678486997	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	1348	0.6809318866056455	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1274	0.643551352771211	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1151	0.5814188438301914	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	922	0.46574124588308985	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	756	0.3818876159301691	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	659	0.33288880806611304	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	583	0.2944979895334505	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	359	0.18134610333191895	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	323	0.16316097876381566	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	303	0.15305813178153604	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.1384090036572306	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	267	0.13487300721343273	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	255	0.128811299024065	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	252	0.12729587197672304	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	251	0.12679072962760907	No Hit
TTTTTTGGGTTAAAAACAAATCTTGTTGCTGATTTGTCCATTTCTTTCCCT	219	0.11062617445596169	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5005960679719546	0.0
2	0.0	0.0	0.0	2.032692812834657	0.0
3	0.0	0.0	0.0	2.620173364854216	0.0
4	0.0	0.0	0.0	3.6829928673900305	0.0
5	0.0	0.0	0.0	5.593946374088218	0.0
6	0.0	0.0	0.0	6.399143278575902	0.0
7	0.0	0.0	0.0	7.288698955365622	0.0
8	0.0	0.0	0.0	8.625305611121213	0.0
9	0.0	0.0	0.0	9.173890202258997	0.0
10	0.0	0.0	0.0	10.900971893879696	0.0
11	0.0	0.0	0.0	12.766967731506739	0.0
12	0.0	0.0	0.0	15.411387929118426	0.0
13	0.0	0.0	0.0	16.045341577256472	0.0
14	0.0	0.0	0.0	16.28376876603827	0.0
15	0.0	0.0	0.0	16.770725990584147	0.0
16	0.0	0.0	0.0	17.62795255703057	0.0
17	0.0	0.0	0.0	18.824129639732476	0.0
18	0.0	0.0	0.0	20.340061829423533	0.0
19	0.0	0.0	0.0	21.471075549089733	0.0
20	0.0	0.0	0.0	22.415691741932875	0.0
21	0.0	0.0	0.0	23.50528378897173	0.0
22	0.0	0.0	0.0	24.535269038815137	0.0
23	0.0	0.0	0.0	25.478874946960055	0.0
24	0.0	0.0	0.0	26.288618132589765	0.0
25	0.0	0.0	0.0	26.937220908852115	0.0
26	0.0	0.0	0.0	27.57774140752864	0.0
27	0.0	0.0	0.0	28.15764482431149	0.0
28	0.0	0.0	0.0	28.7612899315027	0.0
29	0.0	0.0	0.0	29.448788668646824	0.0
30	0.0	0.0	0.0	30.182255359560322	0.0
31	0.0	0.0	0.0	30.847022690994322	0.0
32	0.0	0.0	0.0	31.46733749570629	0.0
33	0.0	0.0	0.0	32.05986947121699	0.0
34	0.0	0.0	0.0	32.695338546402375	0.0
35	0.0	0.0	0.0	33.38586813764119	0.0
36	0.0	0.0	0.0	34.00971893879695	0.0
37	0.0	0.0	0.0	34.65478571861551	0.0
38	0.0	0.0	0.0	35.29631650199026	0.0
39	0.0	0.0	0.0	36.05200945626478	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATT	30	2.156372E-6	45.000004	9
TACCGGG	35	1.2051169E-7	45.000004	3
ACTGCGG	20	7.0191285E-4	45.0	2
GTTCTCG	25	3.879075E-5	45.0	1
ATTAGTC	25	3.879075E-5	45.0	31
GATAAAC	20	7.0191285E-4	45.0	9
CAAGTCG	20	7.0191285E-4	45.0	27
TAAAGCA	20	7.0191285E-4	45.0	13
GGGTCTA	20	7.0191285E-4	45.0	8
GGCCGAT	45	3.8198777E-10	45.0	8
GCAAACG	25	3.879075E-5	45.0	10
TACGGGA	25	3.879075E-5	45.0	4
CGTTCGG	20	7.0191285E-4	45.0	2
ACGGGAA	20	7.0191285E-4	45.0	5
AACATAC	20	7.0191285E-4	45.0	16
TTAACTT	20	7.0191285E-4	45.0	22
CTATTTC	20	7.0191285E-4	45.0	39
ACCCGTC	25	3.879075E-5	45.0	39
GCGTCCC	20	7.0191285E-4	45.0	7
CATCAGA	20	7.0191285E-4	45.0	12
>>END_MODULE