##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934002.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302783
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.670229174028925	31.0	28.0	31.0	25.0	33.0
2	29.432771985217137	31.0	30.0	33.0	25.0	34.0
3	29.559166135483167	31.0	30.0	33.0	25.0	34.0
4	33.70748027465214	35.0	33.0	37.0	28.0	37.0
5	34.314604849017286	35.0	35.0	37.0	32.0	37.0
6	33.41997734351004	35.0	33.0	37.0	28.0	37.0
7	34.25818160200539	35.0	35.0	37.0	32.0	37.0
8	33.85018973984669	35.0	35.0	37.0	28.0	37.0
9	35.55642489835955	37.0	34.0	39.0	30.0	39.0
10	35.139796487913785	37.0	34.0	39.0	30.0	39.0
11	35.3402304620801	37.0	35.0	39.0	30.0	39.0
12	35.270451115155076	37.0	34.0	39.0	30.0	39.0
13	35.05016794205751	37.0	34.0	39.0	28.0	39.0
14	35.725192629705106	38.0	34.0	40.0	27.0	41.0
15	35.980689140407485	38.0	34.0	40.0	29.0	41.0
16	36.312927079789816	38.0	35.0	40.0	31.0	41.0
17	35.975781335147616	38.0	34.0	40.0	30.0	41.0
18	36.1429604700396	38.0	34.0	40.0	30.0	41.0
19	36.102753457096334	38.0	34.0	40.0	30.0	41.0
20	35.92095659267528	38.0	34.0	40.0	29.0	41.0
21	35.77014891853241	38.0	34.0	40.0	29.0	41.0
22	35.98177572717094	38.0	34.0	40.0	30.0	41.0
23	35.87572618013561	38.0	34.0	40.0	29.0	41.0
24	35.802261025222684	38.0	34.0	40.0	29.0	41.0
25	35.47979906401614	37.0	34.0	40.0	28.0	41.0
26	35.48412229220266	37.0	34.0	40.0	28.0	41.0
27	35.34927654458804	37.0	34.0	40.0	27.0	41.0
28	35.260334959360335	37.0	34.0	40.0	27.0	41.0
29	35.39521373392826	37.0	34.0	40.0	27.0	41.0
30	34.93578239201012	37.0	33.0	40.0	26.0	41.0
31	35.194432976752324	37.0	34.0	40.0	27.0	41.0
32	34.998150490615394	37.0	33.0	40.0	26.0	41.0
33	34.92955020592306	37.0	33.0	40.0	26.0	41.0
34	34.953897675893295	37.0	34.0	40.0	26.0	41.0
35	34.9244442389434	37.0	33.0	40.0	26.0	41.0
36	34.721582783709785	37.0	33.0	40.0	25.0	41.0
37	34.770228183220325	37.0	33.0	40.0	25.0	41.0
38	34.62064911174009	37.0	33.0	40.0	25.0	41.0
39	34.62096286779641	37.0	33.0	40.0	25.0	41.0
40	34.43630256652454	37.0	33.0	40.0	25.0	41.0
41	34.4973991274279	37.0	33.0	40.0	25.0	41.0
42	34.66773563905503	37.0	33.0	40.0	26.0	41.0
43	34.65432999871195	37.0	33.0	40.0	26.0	41.0
44	34.681534300142346	37.0	33.0	40.0	26.0	41.0
45	34.74177546295532	37.0	33.0	40.0	26.0	41.0
46	34.58855021583114	37.0	33.0	40.0	26.0	41.0
47	34.45071222624784	36.0	33.0	40.0	26.0	41.0
48	34.44205586178881	36.0	33.0	40.0	26.0	41.0
49	34.560431067794426	36.0	33.0	40.0	26.0	41.0
50	34.32790150041449	36.0	33.0	40.0	26.0	41.0
51	33.286637624965735	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	8.0
13	4.0
14	11.0
15	13.0
16	35.0
17	83.0
18	132.0
19	372.0
20	708.0
21	1350.0
22	1920.0
23	2535.0
24	3131.0
25	3522.0
26	4335.0
27	5173.0
28	6497.0
29	8399.0
30	10939.0
31	13472.0
32	17147.0
33	21840.0
34	29955.0
35	29756.0
36	34348.0
37	41704.0
38	45721.0
39	19665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70229834567991	4.199046842127861	47.2037729991446	15.894881813047629
2	19.09816601328344	13.170818705145267	47.97131939375724	19.759695887814043
3	20.687423005915125	12.814788148608077	46.54521555041069	19.952573295066102
4	18.953507957844394	4.604287559076963	51.89327009772675	24.548934385351888
5	24.25928800494083	5.619866372946962	45.91770343777557	24.203142184336638
6	21.147818734869528	13.805927017038606	47.84812885796098	17.198125390130887
7	76.46334173318846	2.553644028891979	16.5557511485123	4.427263089407265
8	77.04857934560395	9.898508172519593	7.3666619328033605	5.686250549073098
9	71.85277905298514	5.487098020694689	8.786160385490598	13.87396254082957
10	42.90828745339071	25.569797511749336	16.074878708514017	15.44703632634593
11	36.460105091765385	22.178259677722988	23.374165656592343	17.987469573919277
12	27.122394586221816	20.37895126212502	28.178596552646617	24.32005759900655
13	26.306959109329124	23.452439535905253	32.51800794628497	17.722593408480662
14	20.436087891328114	31.04302421205946	28.01181043849886	20.509077458113566
15	17.35731530502043	21.212221293797867	38.8112938969493	22.619169504232403
16	19.10642275160759	21.352585845308354	32.03416308048999	27.506828322594068
17	19.616028640973898	23.549538778597213	30.84981653527444	25.984616045154453
18	21.060627578166542	20.931492190776893	30.910916398873123	27.096963832183445
19	22.831863083462416	24.110006176040265	28.091075126410665	24.967055614086657
20	27.358867571825368	23.699150877030746	30.710773061895814	18.231208489248075
21	24.37488234147888	29.566719399702095	26.40736104735074	19.651037211468278
22	22.085453938959585	21.581462631653693	31.33729436593204	24.995789063454684
23	22.146884072091233	27.70003599937909	27.42393066981964	22.72914925871003
24	22.26346921722818	24.74148152307098	28.64724901992516	24.34780023977568
25	20.21348622610913	28.93095054874283	26.491249508724067	24.364313716423975
26	18.24607061823154	25.603154734578894	30.210084449919577	25.940690197269994
27	22.72485575478148	27.188448492814988	26.72706195526169	23.35963379714185
28	18.606394678697285	27.15277938325467	31.943669228457345	22.297156709590695
29	20.67388195506353	22.439833147831944	30.480575197418613	26.405709699685914
30	20.67685438086022	29.836219338602234	28.62148799635382	20.86543828418372
31	26.364095738532217	23.971292972194608	23.484805950135907	26.179805339137268
32	27.047753671771535	27.377362665671455	24.12718019175449	21.447703470802523
33	24.20875676639706	25.381874147491768	23.219599515164326	27.189769570946847
34	23.480842715740316	24.366625603154734	25.106429356998245	27.046102324106702
35	21.96424502036112	24.863350980735376	26.82977578001407	26.342628218889434
36	22.506217323958083	26.925553944574165	24.21338053985858	26.35484819160917
37	22.130040325909974	27.632661014654058	27.075496312540665	23.161802346895303
38	23.126463506867957	27.204631699930314	23.766856131288744	25.902048661912985
39	24.144354207468716	25.353470967656705	25.20484967782207	25.297325147052508
40	21.548105408824142	25.336627221475442	27.590716783967395	25.524550585733017
41	18.95185661017957	23.502640504916062	25.538091636584614	32.007411248319755
42	22.268423260222665	22.289230240799515	28.897923595446244	26.544422903531572
43	22.263138947695214	23.03530911576938	24.86962610186173	29.83192583467368
44	21.50913360393417	24.22989401650687	28.136652321959954	26.124320057599004
45	22.254221670305135	23.474567594613966	26.127953022461632	28.143257712619267
46	25.167198951063963	25.670529719303925	25.201216712959447	23.961054616672666
47	18.3702519626267	26.359802234603656	31.511016140272076	23.758929662497565
48	22.18387425978341	24.806544621065253	27.38528913446264	25.624291984688703
49	22.172975365195533	21.23534016110548	31.797690094886438	24.79399437881255
50	22.183543990250442	23.2430486520049	27.539194736824722	27.034212620919934
51	20.172532804021362	23.0534739400825	26.2897850936149	30.48420816228124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	170.5
2	276.0
3	446.5
4	617.0
5	491.5
6	366.0
7	381.5
8	397.0
9	470.5
10	544.0
11	582.0
12	620.0
13	634.0
14	648.0
15	560.0
16	472.0
17	450.5
18	429.0
19	557.5
20	686.0
21	895.0
22	1104.0
23	1212.5
24	1321.0
25	1371.5
26	1939.5
27	2457.0
28	2796.0
29	3135.0
30	3392.0
31	3649.0
32	4312.0
33	4975.0
34	5662.5
35	6350.0
36	7010.5
37	7671.0
38	8501.0
39	9331.0
40	10643.5
41	11956.0
42	14262.5
43	16569.0
44	17657.0
45	18745.0
46	19979.5
47	21214.0
48	30376.0
49	39538.0
50	35473.0
51	31408.0
52	30018.5
53	28629.0
54	24676.5
55	20724.0
56	18706.5
57	16689.0
58	15113.5
59	13538.0
60	12200.0
61	10862.0
62	9595.5
63	8329.0
64	7272.5
65	6216.0
66	5094.5
67	3973.0
68	3297.5
69	2622.0
70	2218.0
71	1814.0
72	1594.5
73	1375.0
74	1127.0
75	679.0
76	479.0
77	402.0
78	325.0
79	284.5
80	244.0
81	169.5
82	95.0
83	57.5
84	20.0
85	11.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.965359801584746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77753110215967	25.05880389157462
2	9.987746996800738	6.784748407070691
3	3.576456415327941	3.644268868838986
4	1.7619774292060533	2.3938478938101966
5	1.1522930509198341	1.9569024035678992
6	0.8864538717345192	1.806523482058248
7	0.7305497762782942	1.7369370202981655
8	0.6515984224515073	1.770541989176837
9	0.673584871487457	2.059069726527813
>10	6.63391153782003	40.73027291975585
>50	0.10393594455148944	2.3316435789825136
>100	0.05196797227574472	2.9436618309704987
>500	0.005996304493355161	1.4816748030657552
>1k	0.005996304493355161	5.301103184301923
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	4911	1.6219536763953064	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	3574	1.1803833108199602	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2827	0.9336719696944675	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT	1776	0.5865586905473558	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1420	0.46898273681151187	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA	1109	0.3662689120591314	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	866	0.2860134155484291	TruSeq Adapter, Index 13 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	795	0.2625642787078535	TruSeq Adapter, Index 8 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	781	0.25794050524633155	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	750	0.24770214972439009	TruSeq Adapter, Index 8 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	652	0.21533573549373644	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	521	0.17207042667520964	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGT	334	0.11031002401059505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27280263422979495	0.0
2	0.0	0.0	0.0	1.327022983456799	0.0
3	0.0	0.0	0.0	1.6477146999666428	0.0
4	0.0	0.0	0.0	2.407995164854037	0.0
5	0.0	0.0	0.0	4.298457971550582	0.0
6	0.0	0.0	0.0	4.822595720367392	0.0
7	0.0	0.0	0.0	5.5088958098704355	0.0
8	0.0	0.0	0.0	6.560474002833713	0.0
9	0.0	0.0	0.0	6.9125413249753125	0.0
10	0.0	0.0	0.0	9.040798195407271	0.0
11	0.0	0.0	0.0	10.255529537655681	0.0
12	0.0	0.0	0.0	13.533454652341776	0.0
13	0.0	0.0	0.0	14.143462479729708	0.0
14	0.0	0.0	0.0	14.413953227228742	0.0
15	0.0	0.0	0.0	15.096950621402126	0.0
16	0.0	0.0	0.0	15.735691898158088	0.0
17	0.0	0.0	0.0	16.545182523457395	0.0
18	0.0	0.0	0.0	17.48446907521228	0.0
19	0.0	0.0	0.0	18.815125023531703	0.0
20	0.0	0.0	0.0	19.532470449133537	0.0
21	0.0	0.0	0.0	20.341300535366912	0.0
22	0.0	0.0	0.0	21.22807423138023	0.0
23	0.0	0.0	0.0	22.02138164956421	0.0
24	0.0	0.0	0.0	22.65219645752899	0.0
25	0.0	0.0	0.0	23.186902831400705	0.0
26	0.0	0.0	0.0	23.705756267690063	0.0
27	0.0	0.0	0.0	24.209747574995955	0.0
28	0.0	0.0	0.0	24.79696680460924	0.0
29	0.0	0.0	0.0	25.36668174897534	0.0
30	0.0	0.0	0.0	26.03580782276416	0.0
31	3.302695329658534E-4	0.0	0.0	26.61774273984999	0.0
32	6.605390659317068E-4	0.0	0.0	27.201329004600655	0.0
33	6.605390659317068E-4	0.0	0.0	27.78854823421394	0.0
34	6.605390659317068E-4	0.0	0.0	28.367840995036048	0.0
35	6.605390659317068E-4	0.0	0.0	28.93788620893511	0.0
36	6.605390659317068E-4	0.0	0.0	29.48778498132326	0.0
37	6.605390659317068E-4	0.0	0.0	30.05287615222783	0.0
38	6.605390659317068E-4	0.0	0.0	30.627545139588417	0.0
39	6.605390659317068E-4	0.0	0.0	31.220378951262123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	35	1.2079727E-7	45.000004	2
CGGGATA	45	3.8198777E-10	45.000004	6
CGGACAA	20	7.0250465E-4	45.0	6
AGCGTCG	20	7.0250465E-4	45.0	18
GATACTA	25	3.8839768E-5	45.0	17
TACCCGA	20	7.0250465E-4	45.0	40
CGAACAC	25	3.8839768E-5	45.0	34
CCGATCG	20	7.0250465E-4	45.0	40
GCGAACA	40	6.7866495E-9	45.0	33
TGGAACG	20	7.0250465E-4	45.0	33
TACGGGA	25	3.8839768E-5	45.0	4
GCCGATC	20	7.0250465E-4	45.0	9
ACGATCG	20	7.0250465E-4	45.0	40
TAACCCG	20	7.0250465E-4	45.0	38
ATGAGCG	20	7.0250465E-4	45.0	15
AACGATC	20	7.0250465E-4	45.0	39
CGAATAC	20	7.0250465E-4	45.0	45
TACTCAC	40	6.7866495E-9	45.0	30
CGTAAGA	20	7.0250465E-4	45.0	38
CGATGCG	20	7.0250465E-4	45.0	10
>>END_MODULE