SRR2934001.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934001.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380029
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	4741	1.247536372224225	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	4269	1.1233353244094528	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	3752	0.9872930750021708	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	3366	0.8857218791197514	Illumina Single End Adapter 1 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	2975	0.7828349941714975	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2475	0.6512660875880525	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2430	0.6394248859955425	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGAT	2143	0.563904333616645	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	1255	0.33023795552444685	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	948	0.24945464688221164	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTA	945	0.24866523344271094	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	895	0.23550834278436647	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	873	0.22971931089469486	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	832	0.21893066055485239	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	660	0.17367095669014734	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.17051330293214464	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	554	0.145778348494457	No Hit
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	478	0.12577987469377339	Illumina Single End Adapter 1 (95% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	418	0.10999160590375998	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	401	0.10551826307992283	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	395	0.1039394362009215	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	384	0.10104492025608573	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	383	0.10078178244291884	Illumina Single End Adapter 1 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACCCGT	55	1.8189894E-12	45.000004	38
ATGGGCG	20	7.0273195E-4	45.0	5
TCGTTCG	20	7.0273195E-4	45.0	1
GGTCGGA	25	3.8858605E-5	45.0	9
TCTCTCG	50	2.1827873E-11	45.0	1
CCGATCG	45	3.8380676E-10	45.0	40
TCTTCGT	35	1.209064E-7	45.0	43
CCGTGTA	25	3.8858605E-5	45.0	41
ATTCGCG	20	7.0273195E-4	45.0	1
AGTGGCG	20	7.0273195E-4	45.0	33
TACGGAG	20	7.0273195E-4	45.0	29
ACGGGGT	25	3.8858605E-5	45.0	5
CTGCGCG	25	3.8858605E-5	45.0	1
AGTGCCG	25	3.8858605E-5	45.0	38
CTACGAA	25	3.8858605E-5	45.0	11
GTCGTTG	20	7.0273195E-4	45.0	1
CCCCCGG	20	7.0273195E-4	45.0	2
GGTATGC	25	3.8858605E-5	45.0	8
CGAATAT	25	3.8858605E-5	45.0	14
TAAGGCG	20	7.0273195E-4	45.0	29

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content