##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934001.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 380029 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.780921982269774 31.0 30.0 31.0 25.0 34.0 2 29.77114114975436 31.0 30.0 33.0 25.0 34.0 3 29.83871757155375 31.0 30.0 33.0 25.0 34.0 4 33.817577079643925 35.0 33.0 37.0 28.0 37.0 5 34.330216904499395 35.0 35.0 37.0 32.0 37.0 6 33.713040320607114 35.0 33.0 37.0 29.0 37.0 7 34.46922471706107 35.0 35.0 37.0 32.0 37.0 8 34.31922563804341 35.0 35.0 37.0 31.0 37.0 9 35.83930963163338 37.0 35.0 39.0 31.0 39.0 10 35.25010722865886 37.0 34.0 39.0 30.0 39.0 11 35.32125443058293 37.0 34.0 39.0 30.0 39.0 12 35.431543382215565 37.0 35.0 39.0 30.0 39.0 13 35.44548442355715 37.0 35.0 39.0 30.0 39.0 14 36.10808648813643 38.0 35.0 40.0 30.0 41.0 15 36.25290438361283 38.0 35.0 40.0 30.0 41.0 16 36.45789926558236 38.0 35.0 40.0 31.0 41.0 17 36.176581260903774 38.0 34.0 40.0 30.0 41.0 18 36.332116759510455 38.0 35.0 40.0 30.0 41.0 19 36.333448236845086 38.0 35.0 40.0 30.0 41.0 20 36.290693604961724 38.0 34.0 40.0 30.0 41.0 21 36.08735649121515 38.0 34.0 40.0 30.0 41.0 22 36.319375626596916 38.0 35.0 40.0 30.0 41.0 23 36.31294980119938 38.0 35.0 40.0 30.0 41.0 24 36.18805406955785 38.0 34.0 40.0 30.0 41.0 25 35.77785905812451 38.0 34.0 40.0 29.0 41.0 26 35.88783487575948 38.0 34.0 40.0 30.0 41.0 27 35.77831691791942 38.0 34.0 40.0 29.0 41.0 28 35.74281962692321 38.0 34.0 40.0 29.0 41.0 29 35.834991540119304 38.0 34.0 40.0 29.0 41.0 30 35.355783374426686 38.0 34.0 40.0 27.0 41.0 31 35.52067342229146 38.0 34.0 40.0 29.0 41.0 32 35.50404311249931 38.0 34.0 40.0 29.0 41.0 33 35.46396459217586 38.0 34.0 40.0 28.0 41.0 34 35.43308800117886 38.0 34.0 40.0 28.0 41.0 35 35.49392546358304 38.0 34.0 40.0 29.0 41.0 36 35.25719089858932 38.0 34.0 40.0 27.0 41.0 37 35.17992311113099 38.0 33.0 40.0 27.0 41.0 38 35.14153656694621 37.0 33.0 40.0 27.0 41.0 39 35.09953714058664 37.0 33.0 40.0 27.0 41.0 40 35.1250009867668 37.0 33.0 40.0 27.0 41.0 41 34.9103042136258 37.0 33.0 40.0 26.0 41.0 42 35.26402985035352 38.0 34.0 40.0 27.0 41.0 43 35.213183730715286 37.0 34.0 40.0 27.0 41.0 44 35.15376458112407 37.0 33.0 40.0 27.0 41.0 45 35.27769985974754 37.0 34.0 40.0 27.0 41.0 46 35.13135576495478 37.0 34.0 40.0 27.0 41.0 47 34.952466785429536 37.0 33.0 40.0 26.0 41.0 48 34.962042370450675 37.0 33.0 40.0 27.0 41.0 49 35.04643592988956 37.0 33.0 40.0 27.0 41.0 50 34.857750329580114 37.0 33.0 40.0 26.0 41.0 51 33.74126185106926 36.0 32.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 3.0 12 3.0 13 3.0 14 6.0 15 13.0 16 25.0 17 66.0 18 100.0 19 276.0 20 576.0 21 967.0 22 1407.0 23 1879.0 24 2571.0 25 3558.0 26 4480.0 27 5689.0 28 7366.0 29 9757.0 30 12720.0 31 16284.0 32 20732.0 33 26212.0 34 34250.0 35 39143.0 36 47467.0 37 57382.0 38 62891.0 39 24201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.749087569632845 4.093634959437306 54.55794163077028 15.599335840159567 2 17.744435293096053 9.550060653265934 53.5088111696739 19.19669288396412 3 18.16519265634991 9.33297195740325 53.539861431627585 18.961973954619253 4 18.873296511582012 3.935225995910838 53.18936186448929 24.00211562801786 5 20.445018669627842 4.5667567475113735 52.107076038933876 22.881148543926912 6 19.34194495683224 9.978712150914799 52.68492667664836 17.9944162156046 7 76.20207931499965 1.3948935475976834 16.908446460664845 5.494580676737828 8 76.58126090377313 6.033223780290451 9.356917498401437 8.028597817534976 9 71.79346839320158 4.728323364795845 12.166703067397489 11.311505174605097 10 37.537661599509505 31.08999576348121 17.898370913798683 13.473971723210598 11 25.299911322556962 25.70277531451547 29.535903838917555 19.46140952401001 12 20.455017906528187 21.51572643140392 34.90181012501677 23.12744553705112 13 21.617823902912672 25.16728986472085 35.86594707246026 17.34893915990622 14 18.765673145996754 29.115672751290035 30.997108115433296 21.12154598727992 15 14.855182104523601 27.135034431582856 36.94112817706017 21.068655286833373 16 16.75135318620421 27.969970712761395 29.606424772846285 25.67225132818811 17 17.70180696736302 28.709387967760353 29.56800665212392 24.02079841275271 18 18.379123698454592 25.387536214341537 32.398316970546986 23.835023116656888 19 18.934870759863063 27.77945893602857 29.470119385625832 23.815550918482536 20 20.67526425614887 28.54229545639938 32.72671296137926 18.055727326072486 21 21.748077120430285 30.680816464006693 29.15593283670457 18.415173578858454 22 18.609895560601956 27.491586168423986 29.901665399219535 23.996852871754523 23 18.793039478566108 29.87745672040818 27.12503519468251 24.204468606343198 24 21.006817900739154 27.401593036320914 27.305810872328163 24.28577819061177 25 17.398935344407924 31.28261264271937 25.750929534325014 25.567522478547687 26 19.91663794078873 27.651573958829456 29.0801491465125 23.351638953869312 27 20.901036499846064 28.24942307034463 29.526167739830385 21.32337268997892 28 18.726465611834886 27.40080362288141 31.57206423720295 22.30066652808075 29 21.023658720781835 22.948511824097633 32.609879772333166 23.417949682787366 30 22.861939483565727 28.50650871380868 30.00323659510195 18.628315207523634 31 23.717400514171285 30.003499732915117 24.73074423267698 21.548355520236615 32 25.084401453573278 30.94684879311842 24.842840941086074 19.125908812222224 33 22.979825223864495 29.16987914080241 25.238073936462747 22.61222169887035 34 23.36953232516466 28.043123024821792 26.472716555841792 22.11462809417176 35 22.773262040528486 26.134058190296006 27.271866094429637 23.820813674745875 36 26.648229477224106 28.054174812974797 26.614547837138748 18.68304787266235 37 20.5129082254249 27.872609721889646 33.05510895221154 18.559373100473913 38 24.345247336387484 28.61229011470177 26.135637017175007 20.906825531735738 39 22.304876733091422 29.483276276284176 29.540640319554562 18.67120667106984 40 25.486739169905455 27.098721413365823 26.56981440890037 20.844725007828348 41 21.867804825421217 30.68528980683053 23.538203663404637 23.908701704343617 42 24.538916766878316 26.47350596928129 26.815321988585083 22.17225527525531 43 24.087898555110268 26.63849337813693 27.528162324454186 21.745445742298614 44 20.981556670675133 24.58180823042452 29.970870644082424 24.465764454817922 45 23.31795731378395 22.80168092435051 27.218712256169926 26.661649505695618 46 25.74014088398517 25.75014012088551 29.12330374787187 19.386415247257446 47 19.736125400956244 25.989332393054216 33.17325783032348 21.10128437566607 48 21.186277889318973 26.436666675437927 29.048046333306143 23.329009101936958 49 23.4192653718532 23.00403390267585 33.58585792136916 19.990842804101792 50 23.457946630388733 23.116656886711276 28.701493833365348 24.72390264953464 51 21.131282086367094 22.600117359464672 26.46798007520479 29.800620478963445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89.0 1 164.0 2 239.0 3 582.5 4 926.0 5 652.0 6 378.0 7 565.5 8 753.0 9 1083.5 10 1414.0 11 1703.5 12 1993.0 13 2142.0 14 2291.0 15 2237.0 16 2183.0 17 1988.5 18 1794.0 19 1821.5 20 1849.0 21 1802.0 22 1755.0 23 1867.0 24 1979.0 25 2104.0 26 2794.0 27 3359.0 28 3853.0 29 4347.0 30 5017.0 31 5687.0 32 6290.5 33 6894.0 34 7886.5 35 8879.0 36 9968.0 37 11057.0 38 12956.5 39 14856.0 40 16665.5 41 18475.0 42 21632.5 43 24790.0 44 27437.5 45 30085.0 46 42949.5 47 55814.0 48 55811.5 49 55809.0 50 51114.5 51 46420.0 52 35988.0 53 25556.0 54 20713.5 55 15871.0 56 13417.0 57 10963.0 58 9293.0 59 7623.0 60 6338.0 61 5053.0 62 4234.0 63 3415.0 64 2780.0 65 2145.0 66 1675.0 67 1205.0 68 982.0 69 759.0 70 612.0 71 465.0 72 376.0 73 287.0 74 234.0 75 126.5 76 72.0 77 54.0 78 36.0 79 35.0 80 34.0 81 22.5 82 11.0 83 9.5 84 8.0 85 4.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 380029.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.39128179124917 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71581536242037 31.249079015000564 2 11.623131098018053 9.85438851345429 3 4.361540426439403 5.546738677833534 4 2.2684087712977083 3.8464302176728973 5 1.5046378377903336 3.189176328777294 6 1.1210402677654796 2.851340033411032 7 0.8957933758113353 2.658168059452687 8 0.7125904577469475 2.4166098316884868 9 0.598162333686765 2.2821181239804176 >10 3.0571697113186724 20.506949238604825 >50 0.08026073356738515 2.384838469561765 >100 0.050789995460610915 4.240185384133891 >500 0.005016295847961572 1.6892162811039917 >1k 0.005643332828956769 7.2847618253243125 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC 4741 1.247536372224225 Illumina Single End Adapter 1 (95% over 22bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC 4269 1.1233353244094528 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG 3752 0.9872930750021708 No Hit CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT 3366 0.8857218791197514 Illumina Single End Adapter 1 (95% over 23bp) GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC 2975 0.7828349941714975 No Hit GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC 2475 0.6512660875880525 Illumina Single End Adapter 1 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC 2430 0.6394248859955425 Illumina Single End Adapter 1 (95% over 22bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGAT 2143 0.563904333616645 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC 1255 0.33023795552444685 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC 948 0.24945464688221164 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTA 945 0.24866523344271094 No Hit CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG 895 0.23550834278436647 Illumina Single End Adapter 1 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT 873 0.22971931089469486 No Hit GAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT 832 0.21893066055485239 No Hit TTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT 660 0.17367095669014734 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.17051330293214464 No Hit CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC 554 0.145778348494457 No Hit TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG 478 0.12577987469377339 Illumina Single End Adapter 1 (95% over 21bp) TTTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC 418 0.10999160590375998 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC 401 0.10551826307992283 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC 395 0.1039394362009215 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG 384 0.10104492025608573 No Hit TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG 383 0.10078178244291884 Illumina Single End Adapter 1 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.9778201137281628 0.0 2 0.0 0.0 0.0 3.814182601854069 0.0 3 0.0 0.0 0.0 4.75832107549687 0.0 4 0.0 0.0 0.0 6.367145665199235 0.0 5 0.0 0.0 0.0 9.18324654171129 0.0 6 0.0 0.0 0.0 10.223693454973173 0.0 7 0.0 0.0 0.0 11.51675266887527 0.0 8 0.0 0.0 0.0 13.506074536416957 0.0 9 0.0 0.0 0.0 14.161287691202514 0.0 10 0.0 0.0 0.0 16.547158243186704 0.0 11 0.0 0.0 0.0 19.3311563064924 0.0 12 0.0 0.0 0.0 23.1013948935476 0.0 13 0.0 0.0 0.0 23.934752347847137 0.0 14 0.0 0.0 0.0 24.23078238765989 0.0 15 0.0 0.0 0.0 24.858892347689256 0.0 16 0.0 0.0 0.0 25.793031584431713 0.0 17 0.0 0.0 0.0 27.247394277805114 0.0 18 0.0 0.0 0.0 28.85095611124414 0.0 19 0.0 0.0 0.0 30.630557141691817 0.0 20 0.0 0.0 0.0 31.769943872704452 0.0 21 0.0 0.0 0.0 33.172731554697144 0.0 22 0.0 0.0 0.0 34.63788289841038 0.0 23 0.0 0.0 0.0 36.06014277857742 0.0 24 0.0 0.0 0.0 37.071381394577784 0.0 25 0.0 0.0 0.0 38.00631004475974 0.0 26 0.0 0.0 0.0 38.90439940109834 0.0 27 0.0 0.0 0.0 39.73144154788187 0.0 28 0.0 0.0 0.0 40.55111583589673 0.0 29 0.0 0.0 0.0 41.44973146786166 0.0 30 0.0 0.0 0.0 42.39571190619663 0.0 31 0.0 0.0 0.0 43.28459143907438 0.0 32 0.0 0.0 0.0 44.11637006649492 0.0 33 0.0 0.0 0.0 44.90657291943509 0.0 34 0.0 0.0 0.0 45.714142868044284 0.0 35 0.0 0.0 0.0 46.55592073236516 0.0 36 0.0 0.0 0.0 47.39033073791737 0.0 37 0.0 0.0 0.0 48.1713237673967 0.0 38 0.0 0.0 0.0 49.00810201326741 0.0 39 0.0 0.0 0.0 49.86698383544414 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCCGT 55 1.8189894E-12 45.000004 38 ATGGGCG 20 7.0273195E-4 45.0 5 TCGTTCG 20 7.0273195E-4 45.0 1 GGTCGGA 25 3.8858605E-5 45.0 9 TCTCTCG 50 2.1827873E-11 45.0 1 CCGATCG 45 3.8380676E-10 45.0 40 TCTTCGT 35 1.209064E-7 45.0 43 CCGTGTA 25 3.8858605E-5 45.0 41 ATTCGCG 20 7.0273195E-4 45.0 1 AGTGGCG 20 7.0273195E-4 45.0 33 TACGGAG 20 7.0273195E-4 45.0 29 ACGGGGT 25 3.8858605E-5 45.0 5 CTGCGCG 25 3.8858605E-5 45.0 1 AGTGCCG 25 3.8858605E-5 45.0 38 CTACGAA 25 3.8858605E-5 45.0 11 GTCGTTG 20 7.0273195E-4 45.0 1 CCCCCGG 20 7.0273195E-4 45.0 2 GGTATGC 25 3.8858605E-5 45.0 8 CGAATAT 25 3.8858605E-5 45.0 14 TAAGGCG 20 7.0273195E-4 45.0 29 >>END_MODULE