##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933999.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229488
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.050608310674196	31.0	30.0	31.0	26.0	34.0
2	30.441713727950916	31.0	30.0	33.0	27.0	34.0
3	30.491337237676916	31.0	30.0	34.0	27.0	34.0
4	34.066304120476886	35.0	33.0	37.0	30.0	37.0
5	34.43194415394269	35.0	35.0	37.0	32.0	37.0
6	34.0427255455623	35.0	35.0	37.0	30.0	37.0
7	34.538132712821586	35.0	35.0	37.0	32.0	37.0
8	34.724739419926095	35.0	35.0	37.0	32.0	37.0
9	35.97891393014014	37.0	35.0	39.0	31.0	39.0
10	35.26893780938437	37.0	34.0	39.0	30.0	39.0
11	35.35702956145855	37.0	34.0	39.0	30.0	39.0
12	35.44058077110786	37.0	35.0	39.0	30.0	39.0
13	35.49516750331172	37.0	35.0	39.0	30.0	39.0
14	36.15794725650143	38.0	35.0	40.0	30.0	41.0
15	36.47865247856097	38.0	35.0	40.0	30.0	41.0
16	36.59744300355574	38.0	35.0	40.0	31.0	41.0
17	36.35519940040438	38.0	35.0	40.0	30.0	41.0
18	36.462743149968624	38.0	35.0	40.0	31.0	41.0
19	36.48622585930419	38.0	35.0	40.0	30.0	41.0
20	36.56774646168863	38.0	35.0	40.0	31.0	41.0
21	36.2024593878547	38.0	34.0	40.0	30.0	41.0
22	36.39894024959911	38.0	35.0	40.0	30.0	41.0
23	36.50763874363801	38.0	35.0	40.0	30.0	41.0
24	36.39971588928397	38.0	35.0	40.0	30.0	41.0
25	35.90701038834275	38.0	34.0	40.0	29.0	41.0
26	36.05182841804365	38.0	34.0	40.0	30.0	41.0
27	36.10045405424249	38.0	34.0	40.0	30.0	41.0
28	35.98589032977759	38.0	34.0	40.0	30.0	41.0
29	36.10711235445862	38.0	35.0	40.0	30.0	41.0
30	35.69729571916614	38.0	34.0	40.0	29.0	41.0
31	35.84132939412954	38.0	34.0	40.0	29.0	41.0
32	35.880242104162306	38.0	34.0	40.0	30.0	41.0
33	35.924880603778846	38.0	34.0	40.0	30.0	41.0
34	35.95314787701318	38.0	34.0	40.0	30.0	41.0
35	35.97219898208185	38.0	35.0	40.0	30.0	41.0
36	35.766297148434774	38.0	34.0	40.0	29.0	41.0
37	35.78260736944851	38.0	34.0	40.0	29.0	41.0
38	35.67274541588231	38.0	34.0	40.0	29.0	41.0
39	35.611186641567315	38.0	34.0	40.0	28.0	41.0
40	35.57176410095517	38.0	34.0	40.0	28.0	41.0
41	35.47244300355574	38.0	34.0	40.0	28.0	41.0
42	35.67970874294081	38.0	34.0	40.0	29.0	41.0
43	35.649585163494386	38.0	34.0	40.0	28.0	41.0
44	35.53126525134212	38.0	34.0	40.0	28.0	41.0
45	35.61119535661995	38.0	34.0	40.0	28.0	41.0
46	35.51389615143275	38.0	34.0	40.0	28.0	41.0
47	35.46576727323433	38.0	34.0	40.0	28.0	41.0
48	35.44319964442585	37.0	34.0	40.0	28.0	41.0
49	35.45804137906993	37.0	34.0	40.0	28.0	41.0
50	35.235973122777665	37.0	34.0	40.0	28.0	41.0
51	34.0964712751865	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	2.0
14	7.0
15	7.0
16	9.0
17	34.0
18	70.0
19	152.0
20	240.0
21	371.0
22	622.0
23	907.0
24	1347.0
25	1850.0
26	2527.0
27	3188.0
28	4275.0
29	5346.0
30	6987.0
31	9060.0
32	11454.0
33	14852.0
34	20495.0
35	21535.0
36	26502.0
37	34906.0
38	42294.0
39	20443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.247734086313883	4.521369309070627	55.97155406818657	18.25934253642892
2	18.74128494736108	3.425451439726696	57.38382834832323	20.449435264588995
3	20.62373631736736	3.4189151502475075	55.42032698877502	20.537021543610123
4	20.871243812312628	4.4725650142926865	49.532873178554	25.12331799484069
5	17.680227288572823	5.209422714913198	52.59054939691836	24.51980059959562
6	21.035958307188178	5.83472774175556	54.691748588161474	18.43756536289479
7	83.6244160914732	2.092919891236143	9.667172139719723	4.61549187757094
8	85.39313602454158	2.54566687582793	6.868768737363173	5.192428362267308
9	79.9065746357108	5.6931081363731435	9.317698528899115	5.082618699016942
10	36.82894094680332	36.67163424667085	15.325855818169142	11.17356898835669
11	25.562556647842154	24.856201631457854	31.806891863626856	17.774349857073137
12	24.241354667782193	23.047828208882382	33.83532036533501	18.875496758000416
13	21.701788328801506	26.175224848358088	34.14165446559297	17.98133235724744
14	17.318988356689673	29.516576030119225	32.10015338492644	21.06428222826466
15	15.910635850240535	28.34178693439308	37.35881614724953	18.388761068116853
16	18.924301052778358	25.05664784215297	34.84365195565781	21.17539914941086
17	18.627117757791257	25.971292616607407	31.0373527156104	24.364236909990936
18	19.26113783727254	24.74290594715192	33.84403541797392	22.15192079760162
19	21.22463919682075	26.30812940110158	31.185508610472006	21.281722791605663
20	24.02696437286481	27.02537823328453	31.361116921146206	17.586540472704453
21	22.85696855609008	27.21144460712543	30.61380115735899	19.317785679425505
22	20.63811615422157	25.432702363522274	31.00162099979084	22.927560482465314
23	19.660287248134978	26.856741964721465	30.031199888447325	23.45177089869623
24	20.63201561737433	25.52203165307119	32.12586279021125	21.720089939343236
25	19.02495991075786	28.044167886774034	29.398922819493826	23.531949382974275
26	19.58141602175277	28.944868577006204	29.225493271979364	22.24822212926166
27	19.64024262706547	27.712124381231263	30.753241999581675	21.894390992121593
28	18.849787352715612	27.619309070626784	32.16072300076692	21.37018057589068
29	19.39578540054382	24.473175067977408	32.33066652722583	23.800373004252943
30	20.489088754096073	25.39435613191104	30.332740709753885	23.783814404239003
31	21.339242139022517	28.249407376420553	28.80760649794325	21.60374398661368
32	21.0390085756118	29.663424667084985	28.133497176322948	21.164069580980268
33	20.229815938088265	28.47076971344907	28.545719166143762	22.7536951823189
34	18.999686258105	26.223593390504078	31.39859164749355	23.37812870389737
35	18.546939273513214	26.701614027748725	29.5562295196263	25.19521717911176
36	21.26603569685561	26.995311301680264	28.798019940040437	22.94063306142369
37	20.07120198005996	27.96921843407934	30.934950847103117	21.02462873875758
38	19.327372237328312	29.468207487973224	28.553998466150738	22.650421808547723
39	19.41147249529387	26.313358432684936	30.903140904971067	23.37202816705013
40	20.837690859652792	25.29936205814683	30.824269678588855	23.03867740361152
41	18.100728578400613	27.170048107090565	29.507860977480306	25.221362337028513
42	20.62155755420763	27.27506449138953	27.849822212926167	24.253555741476678
43	22.168043644983616	25.910287248134978	28.414121871296103	23.507547235585303
44	20.152687722233843	25.576064979432477	29.72094401450185	24.550303283831834
45	20.275134211810638	25.11896046852123	28.497350623997768	26.10855469567036
46	20.582775569964443	25.688489158474514	30.75977828906087	22.968956982500174
47	18.107264867879802	26.487659485463293	32.29842083246183	23.10665481419508
48	18.41752074182528	25.657986474238303	30.859565641776477	25.064927142159938
49	19.401450184759117	24.653140904971067	32.47533640103186	23.470072509237955
50	19.20448999511957	24.42785679425504	30.38720978874712	25.98044342187827
51	17.983511120407165	24.978648121034652	28.256815171163634	28.78102558739455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	122.5
2	189.0
3	341.5
4	494.0
5	382.5
6	271.0
7	309.0
8	347.0
9	415.0
10	483.0
11	490.0
12	497.0
13	568.0
14	639.0
15	670.5
16	702.0
17	744.0
18	786.0
19	940.0
20	1094.0
21	1223.5
22	1353.0
23	1384.5
24	1416.0
25	1854.5
26	3004.0
27	3715.0
28	4092.5
29	4470.0
30	5191.5
31	5913.0
32	6298.0
33	6683.0
34	7658.5
35	8634.0
36	9288.5
37	9943.0
38	10819.0
39	11695.0
40	12413.5
41	13132.0
42	14402.0
43	15672.0
44	16736.0
45	17800.0
46	19574.5
47	21349.0
48	23352.5
49	25356.0
50	23577.5
51	21799.0
52	18136.0
53	14473.0
54	12163.0
55	9853.0
56	8442.0
57	7031.0
58	6796.5
59	6562.0
60	5971.5
61	5381.0
62	4549.0
63	3717.0
64	3001.0
65	2285.0
66	1820.5
67	1356.0
68	1074.0
69	792.0
70	668.5
71	545.0
72	428.5
73	312.0
74	266.0
75	146.0
76	72.0
77	76.0
78	80.0
79	49.5
80	19.0
81	12.0
82	5.0
83	3.0
84	1.0
85	1.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	229488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.71242069302099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25959780621572	30.975475841874083
2	9.734134238704623	8.120686048943735
3	3.419169495951946	4.278655093076762
4	1.950378688952729	3.2542006553719585
5	1.4458083050404806	3.015408213065607
6	1.2128493079132934	3.035452834135118
7	1.0822669104204752	3.160078086871645
8	0.9652650822669103	3.22108345534407
9	0.8639331418124837	3.243306839573311
>10	4.964220423086968	31.67485881614725
>50	0.06894750587620789	1.962194101652374
>100	0.029250457038391228	2.059802691208255
>500	0.0010446591799425438	0.3272502265913686
>1k	0.0031339775398276314	1.6715470961444607
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1653	0.7202991006065677	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	1158	0.5046015477933488	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1025	0.44664644774454443	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	751	0.3272502265913686	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	285	0.12418950010458063	No Hit
TTTTTTTGGGGAACTTAAAACAAAAATATAATTATTCATTATTTCTACACA	272	0.11852471588928398	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	267	0.11634595272955449	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	249	0.10850240535452835	No Hit
TTTATTGGGGGAAAAGATCTTTTTGTTAACAATTTTGTTGATGCTTACATT	244	0.10632364219479887	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	243	0.10588788956285296	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5473053057240466	0.0
2	0.0	0.0	0.0	2.2336679913546678	0.0
3	0.0	0.0	0.0	2.8323921076483303	0.0
4	0.0	0.0	0.0	3.7082548978595833	0.0
5	0.0	0.0	0.0	5.0081049989541935	0.0
6	0.0	0.0	0.0	5.685264588998118	0.0
7	0.0	0.0	0.0	6.358502405354528	0.0
8	0.0	0.0	0.0	7.4313253852053265	0.0
9	0.0	0.0	0.0	7.860541727672035	0.0
10	0.0	0.0	0.0	8.71592414418183	0.0
11	0.0	0.0	0.0	10.363069092937321	0.0
12	0.0	0.0	0.0	11.985376141671896	0.0
13	0.0	0.0	0.0	12.4629610262846	0.0
14	0.0	0.0	0.0	12.637697831694904	0.0
15	0.0	0.0	0.0	12.923115805619465	0.0
16	0.0	0.0	0.0	13.608554695670362	0.0
17	0.0	0.0	0.0	14.785522554556229	0.0
18	0.0	0.0	0.0	16.115439587255107	0.0
19	0.0	0.0	0.0	16.952520393223175	0.0
20	0.0	0.0	0.0	17.79221571498292	0.0
21	0.0	0.0	0.0	18.74564247368054	0.0
22	0.0	0.0	0.0	19.840253085128634	0.0
23	0.0	0.0	0.0	20.85599247019452	0.0
24	0.0	0.0	0.0	21.62073833925957	0.0
25	0.0	0.0	0.0	22.297897929303492	0.0
26	0.0	0.0	0.0	22.91535940877083	0.0
27	0.0	0.0	0.0	23.562452067210486	0.0
28	0.0	0.0	0.0	24.24701945199749	0.0
29	0.0	0.0	0.0	24.943352157847034	0.0
30	0.0	0.0	0.0	25.808756884891586	0.0
31	0.0	0.0	0.0	26.568273722373284	0.0
32	0.0	0.0	0.0	27.287265565084013	0.0
33	0.0	0.0	0.0	27.93130795510005	0.0
34	0.0	0.0	0.0	28.603238513560623	0.0
35	0.0	0.0	0.0	29.347504008924215	0.0
36	0.0	0.0	0.0	30.032507146343164	0.0
37	0.0	0.0	0.0	30.770236352227567	0.0
38	0.0	0.0	0.0	31.483999163354948	0.0
39	0.0	0.0	0.0	32.220856863975456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0214743E-4	45.000004	1
CGGCGCA	20	7.0214743E-4	45.000004	22
GATACAA	25	3.8810173E-5	45.000004	9
TCTTGCG	50	2.1827873E-11	45.000004	1
ACCGGCG	20	7.0214743E-4	45.000004	20
CTCGTTT	20	7.0214743E-4	45.000004	18
CACGGGT	20	7.0214743E-4	45.000004	39
ATGGCAC	20	7.0214743E-4	45.000004	21
CCGGCGC	20	7.0214743E-4	45.000004	21
CGGTTGG	20	7.0214743E-4	45.000004	2
CGCGCAT	25	3.8810173E-5	45.000004	36
GTTAGCG	20	7.0214743E-4	45.000004	1
CCTTTCG	20	7.0214743E-4	45.000004	1
AACGCCA	20	7.0214743E-4	45.000004	36
GGAACGA	20	7.0214743E-4	45.000004	8
CGGTGGA	20	7.0214743E-4	45.000004	16
GAACGGC	20	7.0214743E-4	45.000004	39
CGATGAA	20	7.0214743E-4	45.000004	19
GGACCTA	20	7.0214743E-4	45.000004	8
CACACCG	20	7.0214743E-4	45.000004	17
>>END_MODULE