##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933997.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548485
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.996758343436923	31.0	30.0	31.0	26.0	34.0
2	30.15350283052408	31.0	30.0	33.0	26.0	34.0
3	30.23209750494544	31.0	30.0	34.0	25.0	34.0
4	34.003141380347685	35.0	33.0	37.0	28.0	37.0
5	34.42895977100559	35.0	35.0	37.0	32.0	37.0
6	33.842569988240335	35.0	35.0	37.0	29.0	37.0
7	34.390871217991375	35.0	35.0	37.0	32.0	37.0
8	34.464856832912474	35.0	35.0	37.0	31.0	37.0
9	35.91112427869495	37.0	35.0	39.0	31.0	39.0
10	35.29032152201063	37.0	34.0	39.0	30.0	39.0
11	35.38818199221492	37.0	35.0	39.0	30.0	39.0
12	35.288556660619705	37.0	34.0	39.0	30.0	39.0
13	35.15636526067258	37.0	34.0	39.0	29.0	39.0
14	35.73392162046364	38.0	34.0	40.0	27.0	41.0
15	36.03238921757204	38.0	34.0	40.0	29.0	41.0
16	36.28184180059619	38.0	35.0	40.0	30.0	41.0
17	36.0525228584191	38.0	34.0	40.0	30.0	41.0
18	36.222551209239995	38.0	35.0	40.0	30.0	41.0
19	36.19833723802839	38.0	34.0	40.0	30.0	41.0
20	36.12723957811061	38.0	34.0	40.0	30.0	41.0
21	35.86778854480979	38.0	34.0	40.0	29.0	41.0
22	36.109355770896194	38.0	34.0	40.0	30.0	41.0
23	36.16362708187097	38.0	34.0	40.0	30.0	41.0
24	36.08363583324977	38.0	34.0	40.0	30.0	41.0
25	35.653193797460276	38.0	34.0	40.0	29.0	41.0
26	35.750104378424204	38.0	34.0	40.0	29.0	41.0
27	35.70062080093348	38.0	34.0	40.0	29.0	41.0
28	35.60054696117487	38.0	34.0	40.0	29.0	41.0
29	35.74894482073348	38.0	34.0	40.0	29.0	41.0
30	35.31637510597373	37.0	34.0	40.0	27.0	41.0
31	35.42381468955395	38.0	34.0	40.0	28.0	41.0
32	35.43481954839239	38.0	34.0	40.0	28.0	41.0
33	35.41606424970601	38.0	34.0	40.0	28.0	41.0
34	35.340231729217756	38.0	34.0	40.0	27.0	41.0
35	35.3311776985697	38.0	34.0	40.0	27.0	41.0
36	35.119197425636074	37.0	34.0	40.0	27.0	41.0
37	35.12333974493377	37.0	34.0	40.0	27.0	41.0
38	35.05561865866888	37.0	33.0	40.0	27.0	41.0
39	34.996431989935914	37.0	33.0	40.0	27.0	41.0
40	34.936614492647934	37.0	33.0	40.0	26.0	41.0
41	34.738581729673555	37.0	33.0	40.0	26.0	41.0
42	34.957896751962224	37.0	33.0	40.0	27.0	41.0
43	34.87464561473878	37.0	33.0	40.0	26.0	41.0
44	34.83447496285222	37.0	33.0	40.0	26.0	41.0
45	34.928213169001886	37.0	33.0	40.0	27.0	41.0
46	34.833599824972424	37.0	33.0	40.0	27.0	41.0
47	34.7242404076684	36.0	33.0	40.0	26.0	41.0
48	34.715467150423436	36.0	33.0	40.0	27.0	41.0
49	34.72184836413029	36.0	33.0	40.0	27.0	41.0
50	34.4986809119666	36.0	33.0	40.0	26.0	41.0
51	33.374249067887	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	12.0
12	7.0
13	11.0
14	20.0
15	46.0
16	79.0
17	156.0
18	308.0
19	658.0
20	1314.0
21	1876.0
22	2673.0
23	3735.0
24	4691.0
25	5839.0
26	7121.0
27	8786.0
28	11320.0
29	14266.0
30	17855.0
31	22922.0
32	29114.0
33	38846.0
34	51961.0
35	52478.0
36	61378.0
37	76934.0
38	90046.0
39	44030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.42278275613736	4.581529121124552	52.32978112436986	16.665906998368232
2	19.35823222148281	7.139301895220471	53.61276972022936	19.88969616306736
3	21.6324967866031	6.975942824325186	50.27904135938084	21.112519029690876
4	19.345834434852367	4.820368834152255	49.93099173177024	25.902804999225136
5	21.19565712827151	5.651020538392117	49.55650564737413	23.596816685962242
6	22.36031978996691	9.104168755754486	50.821626844854464	17.71388460942414
7	83.28194936962726	2.2892148372334704	10.795190388068953	3.63364540507033
8	85.39212558228574	5.056838382088845	5.738352005980109	3.8126840296452955
9	80.25834799493148	4.537225265959871	7.454169211555467	7.750257527553169
10	44.532484935777646	24.381523651512804	15.874818819110823	15.211172593598732
11	34.919277646608386	24.409418671431308	22.69196058233133	17.979343099628977
12	28.616826348942997	22.078452464515895	27.497379144370406	21.807342042170706
13	26.517224718998694	23.2296234172311	30.79464342689408	19.458508436876123
14	21.784551993217683	26.572650118052454	31.301494115609362	20.341303773120504
15	19.05211628394578	23.642032143085043	35.55211172593599	21.75373984703319
16	24.160369016472647	23.750148135318195	29.009909113284778	23.079573734924384
17	24.457551254820096	23.7268111251903	28.016992260499375	23.79864535949023
18	25.329407367567025	21.49885593954256	30.134461288822852	23.037275404067568
19	25.59559513933836	24.143230899659972	27.387986909395885	22.873187051605786
20	27.651257554901225	26.70957273216223	27.618439884408875	18.02072982852767
21	25.81200944419629	27.377594647073302	28.437605403976406	18.372790504754004
22	24.404678341249078	22.10780604756739	31.013792537626372	22.47372307355716
23	23.55688852019654	24.963125700794006	29.281384176413212	22.198601602596245
24	22.943380402381106	25.452108991130114	29.378196304365662	22.22631430212312
25	23.342115098863232	27.40275486111744	26.053583963098355	23.201546076920973
26	22.04873424068115	26.10554527471125	27.65271611803422	24.19300436657338
27	22.69888875721305	26.1511253726173	28.59075453294074	22.559231337228912
28	19.30262450203743	27.777787906688424	29.512201792209446	23.4073857990647
29	22.499977209951048	25.05209805190661	29.67045589213926	22.77746884600308
30	22.919496431078336	26.550224709882674	29.084295833067447	21.44598302597154
31	24.459374458736338	26.48386008733147	26.716683227435574	22.340082226496623
32	26.551318632232423	25.676545393219502	26.22660601474972	21.545529959798355
33	25.294219531983554	25.126120130906042	25.72011996681769	23.859540370292713
34	22.346463440203472	26.1684458098216	28.791306963727358	22.69378378624757
35	23.041104132291675	27.113594720001462	27.12581018624028	22.719490961466583
36	25.207435025570433	28.04780440668387	24.95637984630391	21.78838072144179
37	21.022999717403394	29.355953216587512	27.273672023847507	22.34737504216159
38	21.865137606315578	27.748252003245305	25.71319179193597	24.67341859850315
39	21.55063493076383	26.313208200771214	27.726191235858778	24.40996563260618
40	23.083584783540115	24.096009918229306	26.982870999206902	25.837534299023673
41	21.660391806521602	25.38228028113804	26.160423712590138	26.79690419975022
42	22.580562823048943	24.19227508500688	27.795837625459217	25.431324466484956
43	23.153960454707057	24.63093794725471	28.07569942660237	24.139402171435865
44	21.86222048004959	25.046810760549516	28.97836768553379	24.112601073867108
45	21.539513386874756	25.03350137196095	26.666362799347294	26.760622441817006
46	22.469894345333053	26.21986016025962	27.275130586980502	24.03511490742682
47	19.35294493012571	27.288986936743942	30.265731970792274	23.092336162338075
48	20.46272915394222	28.523113667648158	27.820815519111736	23.193341659297882
49	23.46299351851008	24.133750239295516	29.36762172165146	23.03563452054295
50	21.985651385179175	26.05121379800724	26.599815856404458	25.363318960409124
51	20.766657246779765	26.01657292359864	26.235904354722557	26.98086547489904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	254.5
2	428.0
3	774.5
4	1121.0
5	865.0
6	609.0
7	634.0
8	659.0
9	734.0
10	809.0
11	804.0
12	799.0
13	812.0
14	825.0
15	913.0
16	1001.0
17	946.0
18	891.0
19	1083.0
20	1275.0
21	1335.0
22	1395.0
23	2096.0
24	2797.0
25	3167.5
26	4294.5
27	5051.0
28	6165.0
29	7279.0
30	8430.0
31	9581.0
32	10606.5
33	11632.0
34	13633.0
35	15634.0
36	17143.5
37	18653.0
38	19677.0
39	20701.0
40	23438.5
41	26176.0
42	27973.5
43	29771.0
44	32958.0
45	36145.0
46	44064.0
47	51983.0
48	50025.5
49	48068.0
50	47168.0
51	46268.0
52	42109.0
53	37950.0
54	36409.5
55	34869.0
56	32970.5
57	31072.0
58	28226.5
59	25381.0
60	22836.0
61	20291.0
62	18021.0
63	15751.0
64	14005.0
65	12259.0
66	10762.0
67	9265.0
68	7897.0
69	6529.0
70	5508.0
71	4487.0
72	3860.0
73	3233.0
74	2504.0
75	1481.5
76	1188.0
77	921.5
78	655.0
79	514.5
80	374.0
81	279.5
82	185.0
83	114.0
84	43.0
85	24.5
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	548485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.219243411371465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63381595710672	24.460596555872566
2	9.240662793441805	6.139356532354943
3	3.22644972496149	3.2154065630414475
4	1.6641125056367365	2.211222335546161
5	1.1624531945597694	1.9307907812203664
6	0.9183064382469862	1.830326705901372
7	0.8198863162206818	1.9065202175731255
8	0.7433294549955358	1.9754273680271037
9	0.675576309531592	2.0197920482357388
>10	7.776945800585339	47.256938090623045
>50	0.09175159950883863	2.0592847189284624
>100	0.04003706160385659	2.2155598240785657
>500	0.002780351500267819	0.5454850892532938
>1k	0.003892492100374946	2.2332931693438267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	3157	0.5755854763576032	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	2669	0.486613125244993	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	1781	0.32471261748270236	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1235	0.2251656836558885	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	1186	0.21623198446630265	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	1059	0.19307729473002908	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1003	0.18286735279907382	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	703	0.12817123531181346	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	662	0.1206960992552212	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.823203916242012E-4	0.0	0.0	0.20766292605996517	0.0
2	1.823203916242012E-4	0.0	0.0	0.7854362471170588	0.0
3	1.823203916242012E-4	0.0	0.0	0.9972925421843806	0.0
4	1.823203916242012E-4	0.0	0.0	1.4250161809347566	0.0
5	1.823203916242012E-4	0.0	0.0	2.3743584601219725	0.0
6	1.823203916242012E-4	0.0	0.0	2.7198556022498335	0.0
7	1.823203916242012E-4	0.0	0.0	3.1362753767195093	0.0
8	1.823203916242012E-4	0.0	0.0	3.7138663773849787	0.0
9	1.823203916242012E-4	0.0	0.0	3.933380128900517	0.0
10	1.823203916242012E-4	0.0	0.0	4.944529020848337	0.0
11	1.823203916242012E-4	0.0	0.0	5.90809229058224	0.0
12	1.823203916242012E-4	0.0	0.0	7.453622250380594	0.0
13	1.823203916242012E-4	0.0	0.0	7.777423265905175	0.0
14	1.823203916242012E-4	0.0	0.0	7.91215803531546	0.0
15	1.823203916242012E-4	0.0	0.0	8.214262924236762	0.0
16	1.823203916242012E-4	0.0	0.0	8.664047330373666	0.0
17	1.823203916242012E-4	0.0	0.0	9.227235020100823	0.0
18	1.823203916242012E-4	0.0	0.0	9.864809429610656	0.0
19	1.823203916242012E-4	0.0	0.0	10.567289898538702	0.0
20	1.823203916242012E-4	0.0	0.0	11.07796931547809	0.0
21	1.823203916242012E-4	0.0	0.0	11.736875210807952	0.0
22	1.823203916242012E-4	0.0	0.0	12.450294903233452	0.0
23	1.823203916242012E-4	0.0	0.0	13.124698031851372	0.0
24	1.823203916242012E-4	0.0	0.0	13.688068041970155	0.0
25	1.823203916242012E-4	0.0	0.0	14.198018177343044	0.0
26	1.823203916242012E-4	0.0	0.0	14.672598156740841	0.0
27	1.823203916242012E-4	0.0	0.0	15.162310728643446	0.0
28	1.823203916242012E-4	0.0	0.0	15.63816695078261	0.0
29	1.823203916242012E-4	0.0	0.0	16.18239331978085	0.0
30	1.823203916242012E-4	0.0	0.0	16.88669699262514	0.0
31	1.823203916242012E-4	0.0	0.0	17.42089574008405	0.0
32	1.823203916242012E-4	0.0	0.0	17.929751953107196	0.0
33	1.823203916242012E-4	0.0	0.0	18.4706965550562	0.0
34	1.823203916242012E-4	0.0	0.0	19.062326225876735	0.0
35	1.823203916242012E-4	0.0	0.0	19.689143732280737	0.0
36	1.823203916242012E-4	0.0	0.0	20.24649716947592	0.0
37	1.823203916242012E-4	0.0	0.0	20.811690383510943	0.0
38	1.823203916242012E-4	0.0	0.0	21.480623900380138	0.0
39	1.823203916242012E-4	0.0	0.0	22.590955085371522	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	80	0.0	45.0	6
GAATCGC	20	7.030059E-4	45.0	18
TAACGCA	25	3.88813E-5	45.0	16
AGTATCG	25	3.88813E-5	45.0	31
TACGCGG	40	6.8030204E-9	45.0	2
CGTACTA	20	7.030059E-4	45.0	34
CGTACCC	20	7.030059E-4	45.0	30
TTACGCG	20	7.030059E-4	45.0	1
TAGTCGA	20	7.030059E-4	45.0	41
AATCGTC	20	7.030059E-4	45.0	33
AATCGCG	20	7.030059E-4	45.0	19
TCGTTCG	30	2.1634187E-6	44.999996	1
CATCGGT	30	2.1634187E-6	44.999996	27
ACCGTCG	30	2.1634187E-6	44.999996	15
ACACGCG	300	0.0	41.25	36
TTTGTCG	205	0.0	40.609756	1
GTTTGCG	145	0.0	40.34483	1
TACGGCT	495	0.0	40.0	7
CGATGAA	540	0.0	40.0	19
CCGATGA	550	0.0	39.68182	18
>>END_MODULE