SRR2933996.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933996.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	670821
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3625	0.5403826057920071	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2269	0.33824224345987974	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2028	0.30231611711619044	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1797	0.26788070140916875	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC	1445	0.21540768699846902	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG	1234	0.18395369256478256	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC	861	0.12835018581708085	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	859	0.12805204368974732	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	854	0.12730668837141354	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	35	1.2109194E-7	45.000004	27
TCACGGG	25	3.8890634E-5	45.0	3
AATGCGA	20	7.0311833E-4	45.0	25
CCGTAAG	20	7.0311833E-4	45.0	25
CGAGACT	25	3.8890634E-5	45.0	26
CCGTTTG	45	3.8380676E-10	45.0	1
AATCGGG	40	6.8084773E-9	45.0	21
GTACGGA	20	7.0311833E-4	45.0	29
GGTCGAT	30	2.1641463E-6	44.999996	26
CGGTTAT	30	2.1641463E-6	44.999996	4
CGTTCAT	90	0.0	42.5	17
GCCGATG	50	1.0804797E-9	40.5	9
CGCAATC	50	1.0804797E-9	40.5	18
ACGGGAC	40	3.4553523E-7	39.375	5
GTTCGGT	80	0.0	39.375	9
TATCGTG	40	3.4553523E-7	39.375	1
CCTTGCG	75	0.0	39.000004	1
ACACGCG	185	0.0	38.918922	36
TTGTTCG	145	0.0	38.793106	1
TTGGGAC	990	0.0	38.409092	5