##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933996.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	670821
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.070501668850557	31.0	30.0	31.0	26.0	34.0
2	30.388614846583515	31.0	30.0	33.0	27.0	34.0
3	30.41394947385368	31.0	30.0	34.0	26.0	34.0
4	34.0912210559896	35.0	33.0	37.0	30.0	37.0
5	34.48329882338209	35.0	35.0	37.0	32.0	37.0
6	34.0202542854204	35.0	35.0	37.0	30.0	37.0
7	34.50058361321425	35.0	35.0	37.0	32.0	37.0
8	34.659542560534035	35.0	35.0	37.0	32.0	37.0
9	35.9810873541526	37.0	35.0	39.0	31.0	39.0
10	35.38071258949854	37.0	34.0	39.0	30.0	39.0
11	35.49663173931645	37.0	35.0	39.0	30.0	39.0
12	35.44727878226829	37.0	35.0	39.0	30.0	39.0
13	35.41361406396043	37.0	35.0	39.0	30.0	39.0
14	35.94554434044253	38.0	34.0	40.0	27.0	41.0
15	36.235029911108924	38.0	35.0	40.0	30.0	41.0
16	36.39944784078018	38.0	35.0	40.0	30.0	41.0
17	36.22816966075898	38.0	35.0	40.0	30.0	41.0
18	36.33523100797381	38.0	35.0	40.0	30.0	41.0
19	36.34357004327533	38.0	35.0	40.0	30.0	41.0
20	36.37510751750467	38.0	35.0	40.0	30.0	41.0
21	36.062369842327534	38.0	34.0	40.0	30.0	41.0
22	36.28451106927183	38.0	34.0	40.0	30.0	41.0
23	36.400582271574685	38.0	35.0	40.0	30.0	41.0
24	36.30341178943414	38.0	35.0	40.0	30.0	41.0
25	35.84354097441791	38.0	34.0	40.0	29.0	41.0
26	35.95754456106771	38.0	34.0	40.0	30.0	41.0
27	36.0119972392039	38.0	34.0	40.0	30.0	41.0
28	35.920804506716394	38.0	34.0	40.0	30.0	41.0
29	36.01507555666862	38.0	34.0	40.0	30.0	41.0
30	35.55571754611141	38.0	34.0	40.0	29.0	41.0
31	35.677033068434056	38.0	34.0	40.0	29.0	41.0
32	35.752385509696325	38.0	34.0	40.0	29.0	41.0
33	35.77971023566644	38.0	34.0	40.0	29.0	41.0
34	35.7174864829813	38.0	34.0	40.0	29.0	41.0
35	35.71684696811817	38.0	34.0	40.0	29.0	41.0
36	35.507916418836025	38.0	34.0	40.0	29.0	41.0
37	35.55617221285559	38.0	34.0	40.0	29.0	41.0
38	35.45833538306046	38.0	34.0	40.0	29.0	41.0
39	35.415098811754554	38.0	34.0	40.0	28.0	41.0
40	35.29524567656647	37.0	34.0	40.0	28.0	41.0
41	35.15618473482494	37.0	34.0	40.0	27.0	41.0
42	35.40216391556019	37.0	34.0	40.0	28.0	41.0
43	35.41732593344573	37.0	34.0	40.0	29.0	41.0
44	35.39079128411305	37.0	34.0	40.0	29.0	41.0
45	35.48324813922045	37.0	34.0	40.0	29.0	41.0
46	35.37305033682607	37.0	34.0	40.0	29.0	41.0
47	35.30496361920691	37.0	34.0	40.0	29.0	41.0
48	35.27558171255819	37.0	34.0	40.0	29.0	41.0
49	35.282119969410616	37.0	34.0	40.0	28.0	41.0
50	35.10819577800933	37.0	34.0	40.0	28.0	41.0
51	33.97714740593989	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	9.0
11	8.0
12	8.0
13	26.0
14	24.0
15	34.0
16	71.0
17	135.0
18	297.0
19	473.0
20	864.0
21	1355.0
22	2065.0
23	2870.0
24	4046.0
25	5696.0
26	7546.0
27	9584.0
28	12233.0
29	15904.0
30	20526.0
31	27102.0
32	34597.0
33	46420.0
34	62830.0
35	64623.0
36	79402.0
37	101061.0
38	117418.0
39	53591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.55468746506147	4.259258431086684	53.8064550751989	17.379599028652947
2	20.344920627112153	4.519834650376181	54.05570189364972	21.079542828861946
3	22.099487046469925	4.5155115895298445	52.08215008176548	21.302851282234755
4	20.51575606607426	4.777876661583344	47.25030969513477	27.45605757720763
5	18.64923727790275	5.54931941605883	50.314465408805034	25.48697789723339
6	22.493630938804838	6.755751534313922	52.055019148178125	18.695598378703114
7	83.04122858407833	2.3857333029228363	10.07973811195535	4.493300001043497
8	84.36408520305714	3.187735625450008	7.192678821921199	5.255500349571644
9	78.53600289794149	5.125659453117896	9.859112937728545	6.479224711212082
10	42.398195643845376	23.34810627574271	17.561614797390064	16.692083283021848
11	30.71877594768202	24.656801143673203	24.76025646185793	19.86416644678685
12	26.93833377309297	21.537936349637235	31.398241855875114	20.12548802139468
13	24.96284403738106	22.032703209947215	32.34111633356738	20.66333641910435
14	19.885483608891192	24.705845523619566	33.8106588791943	21.598011988294942
15	18.92621131419559	23.895495221527057	36.171944527675784	21.00634893660157
16	22.483494106475497	23.528333191715824	31.112174484698603	22.875998217110077
17	22.961565007654798	23.554569698921174	29.26503493480377	24.21883035862026
18	23.507612313866144	22.456214101824482	31.11783918511794	22.91833439919144
19	25.110275319347487	24.00193196098512	28.344073903470523	22.54371881619687
20	25.09208864958014	27.39300051727659	28.63371898017504	18.88119185296823
21	25.616967864750805	25.62278163623381	29.101503977961336	19.658746521054052
22	22.899253303042094	23.909209759384396	29.75055938916641	23.4409775484071
23	22.134816888558944	24.51637620169911	29.505486560498255	23.843320349243687
24	22.633608667587925	24.930644687629037	28.6769495886384	23.758797056144633
25	22.615720139947914	25.465660735129042	27.4099946185346	24.50862450638844
26	20.477892015902903	25.5591282920481	28.5155056266873	25.447474065361696
27	20.316149911824468	25.889767911260975	30.30063161409676	23.4934505628178
28	18.28654738000152	25.88261250020497	31.364551795486427	24.46628832430708
29	20.504873878426586	26.030043782171397	29.870263453290818	23.5948188861112
30	23.209917399723622	25.291098519575268	29.988774948905895	21.510209131795218
31	23.297123971968677	27.078013359748727	27.843046058486543	21.781816609796056
32	23.75298328466163	27.85348103294322	26.537779825020387	21.85575585737477
33	24.087349680466176	26.311489950374245	25.676745361281178	23.924415007878405
34	21.158848634732664	25.57433354054211	30.126814753861314	23.140003070863912
35	20.98682062726122	27.79444889173118	27.60766284895673	23.611067632050876
36	22.986012662096147	27.74182680625681	27.396131009613594	21.87602952203345
37	21.267968653336734	29.049627247805297	28.36896877110287	21.313435327755094
38	22.326075063243398	28.643110457186044	26.522574576526374	22.50823990304418
39	23.123605253860568	25.371149680764315	27.147480475417435	24.35776458995768
40	25.31480081869828	24.48104635961009	27.88776737758657	22.31638544410506
41	20.860706507399144	25.88127086063197	27.01853400534569	26.2394886266232
42	20.167078848157704	26.117697567607458	28.52787852497164	25.1873450592632
43	20.758443757723743	24.454362639213738	29.30111013221113	25.48608347085139
44	21.01544227148524	24.558563312716807	28.97091772618925	25.455076689608703
45	21.70579036732601	23.940961895945417	27.776709435154835	26.576538301573745
46	22.343367306628743	24.940483377831047	29.331073416008145	23.385075899532065
47	19.183209827957086	26.630502026621112	31.1628586463453	23.023429499076506
48	19.59583853218668	27.056994339771713	29.39159626785685	23.955570860184757
49	22.128704974948608	24.40039891416637	30.686427526866332	22.78446858401869
50	21.599949912122607	24.27264501260396	28.70646565924442	25.420939416029015
51	20.53602973073294	24.50832636426111	26.755423577973858	28.2002203270321
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	380.0
2	608.0
3	1127.0
4	1646.0
5	1263.5
6	881.0
7	949.5
8	1018.0
9	1079.0
10	1140.0
11	1213.5
12	1287.0
13	1345.0
14	1403.0
15	1344.5
16	1286.0
17	1392.0
18	1498.0
19	1613.5
20	1729.0
21	1995.5
22	2262.0
23	2989.5
24	3717.0
25	3837.5
26	4944.0
27	5930.0
28	6759.5
29	7589.0
30	9196.5
31	10804.0
32	12399.5
33	13995.0
34	15832.0
35	17669.0
36	18601.0
37	19533.0
38	23069.5
39	26606.0
40	29932.0
41	33258.0
42	37544.5
43	41831.0
44	49793.5
45	57756.0
46	62902.5
47	68049.0
48	68655.5
49	69262.0
50	61019.5
51	52777.0
52	46977.0
53	41177.0
54	38441.5
55	35706.0
56	34306.5
57	32907.0
58	31090.0
59	29273.0
60	26919.0
61	24565.0
62	21268.0
63	17971.0
64	16161.0
65	14351.0
66	12014.5
67	9678.0
68	7960.5
69	6243.0
70	5261.0
71	4279.0
72	3618.5
73	2958.0
74	2491.0
75	1492.5
76	961.0
77	779.0
78	597.0
79	450.0
80	303.0
81	229.5
82	156.0
83	88.0
84	20.0
85	12.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	670821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.35689284041823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31924528561609	26.293030511490656
2	10.782583597900944	7.840424728234717
3	3.9505738858377453	4.3089177427667265
4	2.0225595251438824	2.9413591967609323
5	1.2704107121028354	2.3094093061621104
6	0.9900470769250417	2.159702128963983
7	0.8372795916045795	2.1308619072605786
8	0.7612026849769862	2.2139971558037534
9	0.6753187523284164	2.2097242360376255
>10	6.242298692136588	39.74979674990876
>50	0.09981861884766662	2.4641553876278652
>100	0.0440865565589424	2.946220145346378
>500	0.0020795545546670943	0.5576709041283845
>1k	0.002495465465600513	1.8747298995075137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3625	0.5403826057920071	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2269	0.33824224345987974	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2028	0.30231611711619044	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1797	0.26788070140916875	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC	1445	0.21540768699846902	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG	1234	0.18395369256478256	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC	861	0.12835018581708085	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	859	0.12805204368974732	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	854	0.12730668837141354	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3676092430022316	0.0
2	0.0	0.0	0.0	1.5996815842080079	0.0
3	0.0	0.0	0.0	2.0325839530962804	0.0
4	0.0	0.0	0.0	2.748870414015065	0.0
5	0.0	0.0	0.0	3.948892476532488	0.0
6	0.0	0.0	0.0	4.600631166883565	0.0
7	0.0	0.0	0.0	5.271152811256655	0.0
8	0.0	0.0	0.0	6.2577051106032755	0.0
9	0.0	0.0	0.0	6.701638738202889	0.0
10	0.0	0.0	0.0	7.7566146557725535	0.0
11	0.0	0.0	0.0	9.467801395603297	0.0
12	0.0	0.0	0.0	11.160652394603032	0.0
13	0.0	0.0	0.0	11.707146914005376	0.0
14	0.0	0.0	0.0	11.902131865281499	0.0
15	0.0	0.0	0.0	12.235603834704042	0.0
16	0.0	0.0	0.0	12.9094050424778	0.0
17	0.0	0.0	0.0	13.837372413803385	0.0
18	0.0	0.0	0.0	14.90978964582206	0.0
19	0.0	0.0	0.0	15.714326176431566	0.0
20	0.0	0.0	0.0	16.536453092553753	0.0
21	0.0	0.0	0.0	17.577267259075075	0.0
22	0.0	0.0	0.0	18.68739947020144	0.0
23	0.0	0.0	0.0	19.70391505334508	0.0
24	0.0	0.0	0.0	20.526191040530932	0.0
25	0.0	0.0	0.0	21.281534120130406	0.0
26	0.0	0.0	0.0	21.9058437347668	0.0
27	0.0	0.0	0.0	22.579794013604225	0.0
28	0.0	0.0	0.0	23.275061454545998	0.0
29	0.0	0.0	0.0	24.04203207711148	0.0
30	0.0	0.0	0.0	24.896358342985685	0.0
31	0.0	0.0	0.0	25.61905485964214	0.0
32	0.0	0.0	0.0	26.341304163107594	0.0
33	1.4907106366676058E-4	0.0	0.0	27.07458472528439	0.0
34	1.4907106366676058E-4	0.0	0.0	27.837381358067205	0.0
35	1.4907106366676058E-4	0.0	0.0	28.65667592397972	0.0
36	1.4907106366676058E-4	0.0	0.0	29.40859036911486	0.0
37	1.4907106366676058E-4	0.0	0.0	30.148728200220326	0.0
38	1.4907106366676058E-4	0.0	0.0	31.012445943105536	0.0
39	1.4907106366676058E-4	0.0	0.0	32.200840462656956	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	35	1.2109194E-7	45.000004	27
TCACGGG	25	3.8890634E-5	45.0	3
AATGCGA	20	7.0311833E-4	45.0	25
CCGTAAG	20	7.0311833E-4	45.0	25
CGAGACT	25	3.8890634E-5	45.0	26
CCGTTTG	45	3.8380676E-10	45.0	1
AATCGGG	40	6.8084773E-9	45.0	21
GTACGGA	20	7.0311833E-4	45.0	29
GGTCGAT	30	2.1641463E-6	44.999996	26
CGGTTAT	30	2.1641463E-6	44.999996	4
CGTTCAT	90	0.0	42.5	17
GCCGATG	50	1.0804797E-9	40.5	9
CGCAATC	50	1.0804797E-9	40.5	18
ACGGGAC	40	3.4553523E-7	39.375	5
GTTCGGT	80	0.0	39.375	9
TATCGTG	40	3.4553523E-7	39.375	1
CCTTGCG	75	0.0	39.000004	1
ACACGCG	185	0.0	38.918922	36
TTGTTCG	145	0.0	38.793106	1
TTGGGAC	990	0.0	38.409092	5
>>END_MODULE