##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933995.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177241
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.765838603934757	31.0	30.0	31.0	25.0	34.0
2	29.683718778386492	31.0	30.0	33.0	25.0	34.0
3	29.763243267641233	31.0	30.0	33.0	25.0	34.0
4	33.79334916864608	35.0	33.0	37.0	28.0	37.0
5	34.31756196365401	35.0	35.0	37.0	32.0	37.0
6	33.500465467922204	35.0	33.0	37.0	28.0	37.0
7	34.329506152639624	35.0	35.0	37.0	32.0	37.0
8	33.999853307079064	35.0	35.0	37.0	30.0	37.0
9	35.62715737329399	37.0	35.0	39.0	30.0	39.0
10	35.184206814450384	37.0	34.0	39.0	30.0	39.0
11	35.33493379071434	37.0	34.0	39.0	30.0	39.0
12	35.321184150394096	37.0	34.0	39.0	30.0	39.0
13	35.18922258393938	37.0	34.0	39.0	29.0	39.0
14	35.82164961831631	38.0	34.0	40.0	27.0	41.0
15	36.04681196788553	38.0	34.0	40.0	30.0	41.0
16	36.317635310114476	38.0	35.0	40.0	30.0	41.0
17	36.015724352717484	38.0	34.0	40.0	30.0	41.0
18	36.152814529369614	38.0	34.0	40.0	30.0	41.0
19	36.14917541652326	38.0	34.0	40.0	30.0	41.0
20	36.083795510068214	38.0	34.0	40.0	30.0	41.0
21	35.85805767288607	38.0	34.0	40.0	29.0	41.0
22	36.02541736957025	38.0	34.0	40.0	30.0	41.0
23	36.04013743998285	38.0	34.0	40.0	30.0	41.0
24	35.92849284307807	38.0	34.0	40.0	29.0	41.0
25	35.527744709181285	38.0	34.0	40.0	28.0	41.0
26	35.596222093082304	38.0	34.0	40.0	29.0	41.0
27	35.54479494022264	38.0	34.0	40.0	29.0	41.0
28	35.486704543531125	38.0	34.0	40.0	28.0	41.0
29	35.629239284364225	38.0	34.0	40.0	29.0	41.0
30	35.16760794624269	37.0	34.0	40.0	27.0	41.0
31	35.24759508240193	37.0	34.0	40.0	27.0	41.0
32	35.24456530938101	37.0	34.0	40.0	27.0	41.0
33	35.198368323356334	37.0	34.0	40.0	27.0	41.0
34	35.19984653663656	37.0	34.0	40.0	27.0	41.0
35	35.186277441449775	37.0	34.0	40.0	27.0	41.0
36	34.901078192968896	37.0	33.0	40.0	26.0	41.0
37	34.93581056301871	37.0	33.0	40.0	26.0	41.0
38	34.963755564457436	37.0	33.0	40.0	27.0	41.0
39	34.80409724612251	37.0	33.0	40.0	26.0	41.0
40	34.73069436529923	37.0	33.0	40.0	26.0	41.0
41	34.384651406841535	37.0	33.0	40.0	24.0	41.0
42	34.83337940995594	37.0	33.0	40.0	26.0	41.0
43	34.77910302920882	37.0	33.0	40.0	26.0	41.0
44	34.855027899865156	37.0	33.0	40.0	27.0	41.0
45	34.99231554775701	37.0	33.0	40.0	27.0	41.0
46	34.81153909084241	37.0	33.0	40.0	26.0	41.0
47	34.64395371274141	37.0	33.0	40.0	26.0	41.0
48	34.71316456124712	37.0	33.0	40.0	26.0	41.0
49	34.773274806619234	37.0	33.0	40.0	26.0	41.0
50	34.49795476216	36.0	33.0	40.0	26.0	41.0
51	33.47370529392184	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	4.0
12	2.0
13	8.0
14	2.0
15	11.0
16	14.0
17	38.0
18	92.0
19	116.0
20	290.0
21	465.0
22	803.0
23	1047.0
24	1431.0
25	1764.0
26	2389.0
27	2928.0
28	3722.0
29	4862.0
30	6413.0
31	7901.0
32	10120.0
33	13308.0
34	17400.0
35	18313.0
36	21253.0
37	25264.0
38	26852.0
39	10423.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.31806974684187	4.202187981336147	50.60341568824369	19.87632658357829
2	16.926670465637184	10.384166191795352	53.030619326228134	19.658544016339334
3	19.76799950350088	9.992608933598886	49.721001348446464	20.518390214453767
4	19.29745374941464	4.22532032656101	50.783960821706046	25.693265102318314
5	22.30353022156273	5.551198650425127	47.01113173588504	25.134139392127103
6	20.16181357586563	11.605102656834479	49.45808249784192	18.77500126945797
7	75.87070711629927	2.479674567396934	16.37600780857702	5.273610507726768
8	75.868450302131	7.7640049424230275	9.079163398987818	7.288381356458156
9	70.27719320021892	5.357112631953104	12.050259251527581	12.315434916300404
10	39.837283698467054	26.23151528145294	18.494027905507192	15.437173114572813
11	31.7353208343442	24.952465851580616	25.113263861070518	18.198949453004666
12	26.14914156431074	19.70311609616285	31.672694241174447	22.47504809835196
13	24.394468548473547	23.877093900395508	33.450499602236505	18.277937948894444
14	19.7358399016029	29.2979615326025	29.09315564683115	21.873042918963446
15	15.846784886115515	24.550752929626892	38.4504713920594	21.151990792198195
16	17.15235188246512	23.598941554155076	32.643688537076635	26.605018026303167
17	17.788773477919896	24.94287439136543	31.23882171732274	26.02953041339193
18	19.017608792548	22.976060843709977	32.44678150089426	25.55954886284776
19	20.839986233433574	25.131318374416757	29.73296246353835	24.295732928611326
20	24.672620894713976	24.365129964285916	32.43606163359494	18.52618750740517
21	24.03168567092264	28.085488120694425	28.003678607094294	19.87914760128864
22	20.692729108953344	24.255110273582297	30.21253547429771	24.83962514316665
23	20.873274242415693	26.826750018336615	28.61414683961386	23.685828899633833
24	21.95880185735806	23.719116908615952	29.586269542600192	24.7358116914258
25	18.312918568502774	28.3010138737651	27.2515952855152	26.134472272216925
26	17.49538763604358	26.366359928007626	29.63930467555475	26.49894776039404
27	19.40803764366033	29.265801930704523	27.796615907154663	23.529544518480485
28	16.709452101940293	26.367488335091764	31.796254816887743	25.126804746080193
29	21.33479273982882	23.320225004372578	28.588757680220716	26.756224575577885
30	20.77228180838519	28.446014184077047	27.86826975699753	22.913434250540224
31	22.6781613734971	25.65772027916791	26.083693953430643	25.580424393904345
32	25.722039482963876	27.28883271929181	25.089567312303586	21.899560485440727
33	22.72668287811511	24.74314633747271	27.356537144340194	25.17363364007199
34	21.672750661528653	22.913998454082297	29.013038743857233	26.40021214053182
35	21.75343176804464	24.442990053091552	26.77258647829791	27.0309917005659
36	21.78389875931641	25.55898465930569	27.371206436434008	25.28591014494389
37	21.04986995108355	27.443988693361014	29.8085657381757	21.697575617379723
38	23.093415180460504	25.50256430509871	25.239081250952093	26.164939263488694
39	24.602095451955247	23.688085713802113	30.126212332361025	21.58360650188162
40	26.06169001528992	21.982498406124993	28.520489051630264	23.435322526954824
41	19.57729870628128	23.56170412037847	29.868371313635105	26.99262585970515
42	22.838959382987007	22.982831286214815	28.664360954858076	25.513848375940107
43	22.22849115046744	22.971547215373416	28.699341574466402	26.100620059692737
44	20.865375392826717	23.464096907600386	28.837007238731445	26.833520460841452
45	22.049638627631303	21.99321827342432	26.335892936735856	29.62125016220852
46	25.11721328586501	24.023222617791596	28.704419406345032	22.155144689998362
47	19.95982870780463	23.530108722022558	32.97656862689784	23.533493943274976
48	20.98893596854001	23.013862481028653	30.283625120598508	25.713576429832823
49	22.809056595257303	20.006657601796423	33.8302085860495	23.35407721689677
50	23.257598411202824	20.386366585609426	30.38236074046073	25.97367426272702
51	20.44165853273227	21.447069244700717	27.39828820645336	30.712984016113655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	142.5
2	212.0
3	346.0
4	480.0
5	379.5
6	279.0
7	292.0
8	305.0
9	353.0
10	401.0
11	432.0
12	463.0
13	490.5
14	518.0
15	497.5
16	477.0
17	460.0
18	443.0
19	549.5
20	656.0
21	656.5
22	657.0
23	773.0
24	889.0
25	1124.0
26	1569.5
27	1780.0
28	1981.5
29	2183.0
30	2484.0
31	2785.0
32	3101.0
33	3417.0
34	3630.0
35	3843.0
36	4317.0
37	4791.0
38	5091.5
39	5392.0
40	6459.5
41	7527.0
42	8254.0
43	8981.0
44	9845.0
45	10709.0
46	11547.5
47	12386.0
48	17561.5
49	22737.0
50	22163.5
51	21590.0
52	20314.5
53	19039.0
54	15578.5
55	12118.0
56	10363.0
57	8608.0
58	7515.5
59	6423.0
60	5751.5
61	5080.0
62	4287.0
63	3494.0
64	3097.0
65	2700.0
66	2136.5
67	1573.0
68	1281.5
69	990.0
70	876.5
71	763.0
72	625.0
73	487.0
74	422.0
75	249.5
76	142.0
77	103.5
78	65.0
79	60.0
80	55.0
81	30.0
82	5.0
83	6.0
84	7.0
85	4.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	177241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.10468796723106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70604423096168	33.69592814303688
2	10.02326628640048	9.04192596521121
3	4.0290703492444715	5.451898827020836
4	2.3541479035324726	4.247324264701733
5	1.6724206944861404	3.7717006787368614
6	1.3797158010607424	3.7338990414181823
7	1.1107775442809968	3.5070892175061075
8	0.8555989192434703	3.0873217822061485
9	0.6779745822075454	2.752184878216666
>10	3.097168017612329	20.863682782200506
>50	0.05628940258180727	1.714614564350235
>100	0.02751926348443911	2.308156690607704
>500	0.002501751225858101	0.8637956229089206
>1k	0.007505253677574302	4.960477541878007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	2245	1.2666369519467844	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	1707	0.963095446313212	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1542	0.8700018618716889	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	1179	0.665195976100338	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	1115	0.6290869494078684	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	1004	0.5664603562381165	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGGTGTT	879	0.4959349134793868	No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	652	0.3678607094295338	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTA	490	0.27645973561422016	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTC	331	0.18675137242511608	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	307	0.17321048741543998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	278	0.1568485846954147	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	242	0.1365372571809006	No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG	235	0.13258783238641172	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	228	0.12863840759192285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6973555779983187	0.0
2	0.0	0.0	0.0	2.761212134889783	0.0
3	0.0	0.0	0.0	3.401583155139048	0.0
4	0.0	0.0	0.0	4.526605018026303	0.0
5	0.0	0.0	0.0	6.666064849555125	0.0
6	0.0	0.0	0.0	7.497136667023995	0.0
7	0.0	0.0	0.0	8.497469547113816	0.0
8	0.0	0.0	0.0	9.893873313736664	0.0
9	0.0	0.0	0.0	10.450178006217524	0.0
10	0.0	0.0	0.0	12.57045491731597	0.0
11	0.0	0.0	0.0	14.441918066361621	0.0
12	0.0	0.0	0.0	17.848579053379297	0.0
13	0.0	0.0	0.0	18.63282197685637	0.0
14	0.0	0.0	0.0	18.968523084387925	0.0
15	0.0	0.0	0.0	19.652901980918635	0.0
16	0.0	0.0	0.0	20.636308754746363	0.0
17	0.0	0.0	0.0	21.996039291134668	0.0
18	0.0	0.0	0.0	23.492871288245947	0.0
19	0.0	0.0	0.0	25.0500730643587	0.0
20	0.0	0.0	0.0	26.12149559074932	0.0
21	0.0	0.0	0.0	27.36500019747124	0.0
22	0.0	0.0	0.0	28.57916622000553	0.0
23	0.0	0.0	0.0	29.68218414475206	0.0
24	0.0	0.0	0.0	30.558956449128587	0.0
25	0.0	0.0	0.0	31.295242071529724	0.0
26	0.0	0.0	0.0	32.014037384126695	0.0
27	0.0	0.0	0.0	32.70462251962018	0.0
28	0.0	0.0	0.0	33.435830310142684	0.0
29	0.0	0.0	0.0	34.182835799843154	0.0
30	0.0	0.0	0.0	35.03196213065826	0.0
31	0.0	0.0	0.0	35.820154478929815	0.0
32	0.0	0.0	0.0	36.516381649844	0.0
33	0.0	0.0	0.0	37.22220028097336	0.0
34	0.0	0.0	0.0	37.92689050501859	0.0
35	0.0	0.0	0.0	38.678409623055614	0.0
36	0.0	0.0	0.0	39.38817767897947	0.0
37	0.0	0.0	0.0	40.11374343408128	0.0
38	0.0	0.0	0.0	40.80037914478027	0.0
39	0.0	0.0	0.0	41.50281255465722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGT	30	2.1550823E-6	45.000004	10
GCTTGCG	30	2.1550823E-6	45.000004	1
TGGTTGA	30	2.1550823E-6	45.000004	14
CCTCACC	30	2.1550823E-6	45.000004	30
TTACGGG	60	0.0	45.000004	3
TTGTGCG	25	3.877417E-5	45.0	1
CCGATCG	20	7.0171274E-4	45.0	40
CGTGCGG	20	7.0171274E-4	45.0	2
TTAGCGG	25	3.877417E-5	45.0	2
GGCACGA	25	3.877417E-5	45.0	8
TTTGTCG	55	1.8189894E-12	45.0	1
TTTCGAG	20	7.0171274E-4	45.0	1
GCACGAT	25	3.877417E-5	45.0	9
CACGATC	25	3.877417E-5	45.0	10
ATATGGC	25	3.877417E-5	45.0	23
TGCGGGC	45	3.8016879E-10	45.0	4
CGGGCTC	20	7.0171274E-4	45.0	6
GTACTTA	20	7.0171274E-4	45.0	19
TACTCAC	25	3.877417E-5	45.0	30
CGCACTT	20	7.0171274E-4	45.0	35
>>END_MODULE