##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933994.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1075013
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.113162352455273	31.0	30.0	31.0	26.0	34.0
2	30.39690868854609	31.0	30.0	33.0	27.0	34.0
3	30.44179837825217	31.0	30.0	34.0	26.0	34.0
4	34.13206630989579	35.0	33.0	37.0	30.0	37.0
5	34.51918162850124	35.0	35.0	37.0	32.0	37.0
6	34.02896988222468	35.0	35.0	37.0	30.0	37.0
7	34.48198486902019	35.0	35.0	37.0	32.0	37.0
8	34.63336722439636	35.0	35.0	37.0	32.0	37.0
9	35.98070627983103	37.0	35.0	39.0	31.0	39.0
10	35.35584871996897	37.0	34.0	39.0	30.0	39.0
11	35.49751584399444	37.0	35.0	39.0	30.0	39.0
12	35.475433320341246	37.0	35.0	39.0	30.0	39.0
13	35.46176092754227	37.0	35.0	39.0	30.0	39.0
14	36.04353156659501	38.0	35.0	40.0	29.0	41.0
15	36.30653768838144	38.0	35.0	40.0	30.0	41.0
16	36.458543292034605	38.0	35.0	40.0	31.0	41.0
17	36.24421844200954	38.0	35.0	40.0	30.0	41.0
18	36.361719346649764	38.0	35.0	40.0	30.0	41.0
19	36.33106483363457	38.0	35.0	40.0	30.0	41.0
20	36.35717893644077	38.0	35.0	40.0	30.0	41.0
21	36.06402620247383	38.0	34.0	40.0	30.0	41.0
22	36.27745617959969	38.0	34.0	40.0	30.0	41.0
23	36.37364571405183	38.0	35.0	40.0	30.0	41.0
24	36.27850081812964	38.0	35.0	40.0	30.0	41.0
25	35.83642802459133	38.0	34.0	40.0	29.0	41.0
26	35.94031234971112	38.0	34.0	40.0	30.0	41.0
27	35.993299615911624	38.0	34.0	40.0	30.0	41.0
28	35.876853582235746	38.0	34.0	40.0	29.0	41.0
29	35.95790469510601	38.0	34.0	40.0	30.0	41.0
30	35.519385346967894	38.0	34.0	40.0	28.0	41.0
31	35.612168410986655	38.0	34.0	40.0	29.0	41.0
32	35.636432303609354	38.0	34.0	40.0	29.0	41.0
33	35.66014364477453	38.0	34.0	40.0	29.0	41.0
34	35.57087216619706	38.0	34.0	40.0	29.0	41.0
35	35.558989519196516	38.0	34.0	40.0	29.0	41.0
36	35.34124331519712	38.0	34.0	40.0	28.0	41.0
37	35.39811239491988	38.0	34.0	40.0	28.0	41.0
38	35.271158581338085	37.0	34.0	40.0	28.0	41.0
39	35.20014827727665	37.0	34.0	40.0	27.0	41.0
40	35.09401932813836	37.0	34.0	40.0	27.0	41.0
41	34.96383950705712	37.0	33.0	40.0	27.0	41.0
42	35.17288256048997	37.0	34.0	40.0	28.0	41.0
43	35.129672850467855	37.0	34.0	40.0	27.0	41.0
44	35.07137774147848	37.0	33.0	40.0	27.0	41.0
45	35.15238141306198	37.0	34.0	40.0	28.0	41.0
46	35.061563906669036	37.0	34.0	40.0	28.0	41.0
47	35.00410785730033	37.0	33.0	40.0	28.0	41.0
48	34.95436334258283	36.0	33.0	40.0	28.0	41.0
49	34.94433276620841	36.0	33.0	40.0	28.0	41.0
50	34.76124009663139	36.0	33.0	40.0	27.0	41.0
51	33.59947740166863	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	12.0
11	14.0
12	18.0
13	16.0
14	33.0
15	64.0
16	133.0
17	216.0
18	497.0
19	1004.0
20	1736.0
21	2879.0
22	3920.0
23	5562.0
24	7542.0
25	10175.0
26	13012.0
27	16352.0
28	20367.0
29	26430.0
30	33522.0
31	42896.0
32	55659.0
33	73492.0
34	99114.0
35	103267.0
36	126541.0
37	159049.0
38	184228.0
39	87257.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.992721018257456	4.269901852349692	54.22046058977892	16.51691653961394
2	19.8127836593604	5.081892032933555	53.97534727487017	21.129977032835882
3	21.357323120743658	5.085519896038466	52.296483856474296	21.260673126743583
4	20.161058517431883	4.475666805889789	47.76463168352383	27.598642993154503
5	19.291301593562125	5.261703811953902	50.048418019130935	25.398576575353044
6	22.64028435004972	7.201215241118014	51.74951372681075	18.40898668202152
7	84.1225175881594	2.296809433932427	9.629557968136199	3.951115009771975
8	86.22639912261526	3.552701223147999	6.0711823950035955	4.149717259233144
9	80.82646442415114	5.033334480606281	8.011903111869344	6.1282979833732245
10	42.652879546572926	27.114555823976083	14.994237278991045	15.238327350459949
11	32.29309785090971	23.239253850883664	24.316636170911423	20.151012127295203
12	26.990092212838356	21.157232517188167	30.219448508994773	21.633226760978705
13	26.610468896655203	21.44662436640301	31.457852137602053	20.485054599339726
14	21.48485646220092	23.734038565115025	32.970391985957384	21.81071298672667
15	19.93213105329889	21.991734053448656	35.586453373122	22.489681520130453
16	25.301275426436703	22.179173647202404	29.96168418428428	22.55786674207661
17	25.339228455841926	22.378706117972527	28.572770747888633	23.709294678296914
18	26.121730620932027	21.158906915544275	29.382063286676534	23.337299176847164
19	25.61392280837534	23.483157878090775	27.29846057675582	23.604458736778067
20	27.493341940981182	25.08239435244039	27.5602248530948	19.86403885348363
21	26.887209736068307	25.06360388199957	28.186728904673714	19.862457477258413
22	25.3878790303001	21.233789730914882	29.881592129583552	23.49673910920147
23	24.50528505236681	23.381112600498785	28.82616303244705	23.287439314687358
24	24.452727548411044	24.0651973511018	28.167938434232887	23.314136666254267
25	24.55486584813393	25.04667385417665	26.236705974718443	24.161754322970978
26	22.46614692101398	24.125661736183655	27.517806761406604	25.890384581395757
27	22.384566512218925	23.331066694077187	29.887080435306363	24.397286358397526
28	20.318265918644705	24.89402453737769	29.422062802961452	25.365646741016157
29	21.917130304470735	24.471518018851864	29.63396721714063	23.97738445953677
30	24.583330620187848	23.52520388125539	29.816662682218727	22.074802816338035
31	25.525551784024934	23.95310568337313	27.75268764191689	22.768654890685042
32	25.964709263980996	24.356728709327236	27.18283406805313	22.49572795863864
33	25.52145881026555	23.845944188581907	26.3971691505126	24.235427850639944
34	22.694795318754284	23.90547835235481	29.61629301227055	23.783433316620357
35	22.25061464372989	25.48899408658314	29.019742086839877	23.240649182847093
36	24.433099878792163	26.425633922566515	27.068323824921187	22.07294237372013
37	23.084092936550533	26.11726555864906	27.87436058912776	22.92428091567265
38	24.054406783917962	26.85009390584114	26.43195942746739	22.663539882773513
39	23.10651127009627	24.97932583140855	26.881349341821913	25.03281355667327
40	23.692364650473994	24.78118869260186	27.43101711328142	24.095429543642727
41	21.406810894379884	24.49049453355448	26.803768884655348	27.298925687410293
42	22.432007798975455	24.699701305937698	28.49974837513593	24.36854251995092
43	23.281578920440964	22.99693119990177	29.064206665407767	24.6572832142495
44	23.264183781963567	22.45144942433254	29.363272816235707	24.92109397746818
45	22.521774155289283	22.546518042107397	27.876593120269245	27.055114682334075
46	22.925118114850704	23.86947878769838	28.835000134882087	24.370402962568825
47	20.191383732103706	25.33457734929717	31.038601393657565	23.43543752494156
48	21.234533907962046	25.811873902920247	29.588107306609317	23.365484882508397
49	23.235067854993382	23.619249255590397	30.1036359560303	23.042046933385922
50	22.32214866238827	23.912734078564633	28.347285102598757	25.417832156448338
51	21.37629963544627	24.358868218337825	27.207577954871244	27.057254191344665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	544.5
2	901.0
3	2379.0
4	3857.0
5	2793.5
6	1730.0
7	1725.5
8	1721.0
9	1773.0
10	1825.0
11	1903.0
12	1981.0
13	2033.5
14	2086.0
15	1921.5
16	1757.0
17	1929.0
18	2101.0
19	2226.5
20	2352.0
21	2628.5
22	2905.0
23	3434.5
24	3964.0
25	4927.0
26	7538.5
27	9187.0
28	10506.5
29	11826.0
30	13629.0
31	15432.0
32	17707.0
33	19982.0
34	22623.0
35	25264.0
36	28183.0
37	31102.0
38	33788.0
39	36474.0
40	41135.0
41	45796.0
42	51048.5
43	56301.0
44	68762.0
45	81223.0
46	86336.0
47	91449.0
48	92154.5
49	92860.0
50	85626.0
51	78392.0
52	75008.5
53	71625.0
54	68523.5
55	65422.0
56	64073.5
57	62725.0
58	60778.0
59	58831.0
60	53706.5
61	48582.0
62	44138.0
63	39694.0
64	36491.5
65	33289.0
66	28332.0
67	23375.0
68	19645.0
69	15915.0
70	13359.5
71	10804.0
72	8821.0
73	6838.0
74	5783.5
75	3592.0
76	2455.0
77	1900.0
78	1345.0
79	935.5
80	526.0
81	348.5
82	171.0
83	138.5
84	106.0
85	64.5
86	23.0
87	16.5
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1075013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.225213311132705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20513688701202	23.590511516546318
2	9.692148106015477	6.246630803188792
3	3.4421514364983126	3.3277219287113997
4	1.724090682377308	2.2223676002938033
5	1.132209661479218	1.8242848927046573
6	0.8716008547486394	1.6852514079864298
7	0.7748197187535141	1.7478111500152687
8	0.706017827442255	1.8201260072631322
9	0.6226837130423246	1.805950393034135
>10	7.641557879175213	47.39221431797475
>50	0.126128944700594	2.6779653342907004
>100	0.054723580939844085	3.0289403322067017
>500	0.00409695258318846	0.9042781171005699
>1k	0.0026337552320497247	1.7259461986833486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	2927	0.272275777130137	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	2596	0.24148545180383865	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	2335	0.21720667564020155	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2188	0.20353242239861286	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1970	0.1832535978634677	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1923	0.17888155771139513	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	1786	0.166137525778758	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1463	0.13609137749962094	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT	1114	0.10362665381720966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18027688967482253	0.0
2	0.0	0.0	0.0	0.7368282988205724	0.0
3	0.0	0.0	0.0	0.9790579276715723	0.0
4	0.0	0.0	0.0	1.4435174272311126	0.0
5	0.0	0.0	0.0	2.309553465865064	0.0
6	0.0	0.0	0.0	2.8048032907509026	0.0
7	0.0	0.0	0.0	3.281448689457709	0.0
8	0.0	0.0	0.0	3.9891610613080957	0.0
9	0.0	0.0	0.0	4.321529134996507	0.0
10	0.0	0.0	0.0	5.175472296614087	0.0
11	0.0	0.0	0.0	6.282156587873821	0.0
12	0.0	0.0	0.0	7.627907755534119	0.0
13	0.0	0.0	0.0	8.006507828277426	0.0
14	0.0	0.0	0.0	8.16194780900324	0.0
15	0.0	0.0	0.0	8.424084173865804	0.0
16	0.0	0.0	0.0	8.855706861219353	0.0
17	0.0	0.0	0.0	9.509931507805021	0.0
18	0.0	0.0	0.0	10.196992966596683	0.0
19	0.0	0.0	0.0	10.801357751022545	0.0
20	0.0	0.0	0.0	11.399024942023956	0.0
21	0.0	0.0	0.0	12.147853095730005	0.0
22	0.0	0.0	0.0	12.957145634517909	0.0
23	0.0	0.0	0.0	13.693322778422214	0.0
24	0.0	0.0	0.0	14.281315667810528	0.0
25	0.0	0.0	0.0	14.806983729499086	0.0
26	0.0	0.0	0.0	15.303163775693875	0.0
27	0.0	0.0	0.0	15.805948393182222	0.0
28	0.0	0.0	0.0	16.328081613896764	0.0
29	0.0	0.0	0.0	16.90277233856707	0.0
30	0.0	0.0	0.0	17.61597301614027	0.0
31	0.0	0.0	0.0	18.21494251697421	0.0
32	0.0	0.0	0.0	18.79921452112672	0.0
33	0.0	0.0	0.0	19.374463378582398	0.0
34	0.0	0.0	0.0	19.97687469825946	0.0
35	0.0	0.0	0.0	20.65035492594043	0.0
36	0.0	0.0	0.0	21.259835927565526	0.0
37	0.0	0.0	0.0	21.87322385868822	0.0
38	0.0	0.0	0.0	22.566982910904333	0.0
39	0.0	0.0	0.0	23.652179089927284	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAA	40	6.8139343E-9	45.0	30
GCGTACG	20	7.0330827E-4	45.0	1
TCCCGTA	45	1.9281288E-8	40.0	29
CCGATGA	445	0.0	39.94382	18
GCGTTAG	40	3.4579352E-7	39.375	1
ACACGCG	790	0.0	39.303795	36
GATCGTA	35	6.247912E-6	38.571426	9
CACGCGG	820	0.0	38.140244	37
CGTTTTT	1310	0.0	38.129772	1
TGGGCGA	1570	0.0	38.121017	6
TTTGGGC	2345	0.0	38.091686	4
CCGTTTG	130	0.0	38.07692	1
TCGTTTG	445	0.0	37.92135	1
TTACACG	840	0.0	37.76786	34
TACACGC	840	0.0	37.500004	35
CGCGGAT	30	1.139873E-4	37.499996	23
CGTCGTT	30	1.139873E-4	37.499996	11
TACCGGT	60	1.546141E-10	37.499996	40
TTCGGGC	505	0.0	37.425743	4
CTTGGGA	2830	0.0	37.287987	4
>>END_MODULE