##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933993.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	755555
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.973489686389474	31.0	30.0	31.0	26.0	34.0
2	29.738670249022242	31.0	30.0	33.0	25.0	34.0
3	29.894044775032924	31.0	30.0	34.0	25.0	34.0
4	33.89821389574551	35.0	33.0	37.0	28.0	37.0
5	34.43510134933923	35.0	35.0	37.0	32.0	37.0
6	33.753209230300904	35.0	33.0	37.0	28.0	37.0
7	34.41085691974774	35.0	35.0	37.0	32.0	37.0
8	34.22432781200575	35.0	35.0	37.0	30.0	37.0
9	35.89012580156309	37.0	35.0	39.0	31.0	39.0
10	35.2860360926736	37.0	34.0	39.0	30.0	39.0
11	35.343976282335504	37.0	35.0	39.0	30.0	39.0
12	35.42290104625077	37.0	35.0	39.0	30.0	39.0
13	35.37833513112877	37.0	35.0	39.0	30.0	39.0
14	36.04735724070385	38.0	35.0	40.0	29.0	41.0
15	36.28242285472269	38.0	35.0	40.0	30.0	41.0
16	36.54417481189324	38.0	35.0	40.0	31.0	41.0
17	36.21730118919205	38.0	34.0	40.0	30.0	41.0
18	36.3942929369801	38.0	35.0	40.0	30.0	41.0
19	36.361157030262525	38.0	35.0	40.0	30.0	41.0
20	36.17855616070306	38.0	34.0	40.0	30.0	41.0
21	35.957105703754195	38.0	34.0	40.0	29.0	41.0
22	36.20231485464328	38.0	34.0	40.0	30.0	41.0
23	36.07915373467186	38.0	34.0	40.0	30.0	41.0
24	36.110512140082456	38.0	34.0	40.0	30.0	41.0
25	35.72403729708625	38.0	34.0	40.0	29.0	41.0
26	35.821003103678756	38.0	34.0	40.0	29.0	41.0
27	35.68091535361423	38.0	34.0	40.0	29.0	41.0
28	35.512572876891824	38.0	34.0	40.0	27.0	41.0
29	35.673103877282266	38.0	34.0	40.0	29.0	41.0
30	35.249574154098646	38.0	34.0	40.0	27.0	41.0
31	35.37769321889207	38.0	34.0	40.0	27.0	41.0
32	35.26418195895732	38.0	34.0	40.0	26.0	41.0
33	35.19443720179206	38.0	34.0	40.0	26.0	41.0
34	35.091167419976045	38.0	34.0	40.0	26.0	41.0
35	35.15599129117007	38.0	34.0	40.0	26.0	41.0
36	34.84508870962405	38.0	33.0	40.0	25.0	41.0
37	34.81667780638074	38.0	33.0	40.0	25.0	41.0
38	34.717370674537264	38.0	33.0	40.0	24.0	41.0
39	34.71347949520551	37.0	33.0	40.0	24.0	41.0
40	34.862634751937314	37.0	33.0	40.0	26.0	41.0
41	34.63582134986864	37.0	33.0	40.0	24.0	41.0
42	34.68822918252146	38.0	33.0	40.0	24.0	41.0
43	34.745127753770404	38.0	33.0	40.0	25.0	41.0
44	34.82250001654413	37.0	33.0	40.0	26.0	41.0
45	34.737516130526565	37.0	33.0	40.0	25.0	41.0
46	34.75908438167969	37.0	33.0	40.0	25.0	41.0
47	34.56073085347857	37.0	33.0	40.0	24.0	41.0
48	34.601829118992	37.0	33.0	40.0	25.0	41.0
49	34.53028700756398	37.0	33.0	40.0	24.0	41.0
50	34.38891940361721	37.0	33.0	40.0	24.0	41.0
51	33.36935630099728	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	15.0
11	16.0
12	10.0
13	11.0
14	19.0
15	26.0
16	74.0
17	206.0
18	535.0
19	1182.0
20	2410.0
21	4235.0
22	5912.0
23	7812.0
24	8896.0
25	10071.0
26	11017.0
27	12144.0
28	14393.0
29	18067.0
30	23438.0
31	29527.0
32	37400.0
33	48349.0
34	64759.0
35	67717.0
36	81586.0
37	104096.0
38	130517.0
39	71108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.02146104519195	3.7499586396754707	52.14617069571374	17.082409619418836
2	17.069571374684834	11.918788175579541	52.51980332338479	18.491837126350827
3	19.085572857038866	11.755993938230837	50.859831514582	18.298601690148303
4	17.334806863828575	4.066679460793721	54.99056984600724	23.607943829370463
5	23.891046978711014	4.87628299726691	48.80438882675649	22.428281197265587
6	19.776720424059135	12.084758885852121	51.402743678487994	16.735777011600746
7	80.44708856403571	1.2873980054397098	15.033584583518076	3.2319288470065053
8	83.24569356300997	7.972814685893151	5.499268749462316	3.28222300163456
9	78.30971934538186	3.1489434918702144	6.924710974052187	11.616626188695726
10	44.895077128733185	19.4363084090503	17.524601121030237	18.14401334118628
11	33.67789241021501	25.698327719358616	23.392473082700796	17.23130678772558
12	25.391930435242966	23.6328262006075	27.527314358319384	23.44792900583015
13	27.564373209097948	23.128958183057488	31.325052444891504	17.98161616295306
14	20.504132723627002	30.082654472540053	30.211831038111058	19.201381765721887
15	18.31779288073006	24.10109125080239	34.249789889551394	23.331325978916162
16	20.525838621940164	24.45010621331339	29.43862458722396	25.585430577522484
17	21.811383684840948	27.413755451290772	29.291448008417653	21.483412855450627
18	21.907869049903713	22.66863431517229	30.21288986241902	25.210606772504978
19	22.655134305245813	24.3231796493968	28.116550085698595	24.905135959658793
20	26.78666675490203	26.172283950208787	29.045271356817175	17.995777938072013
21	23.765841004294856	29.64853650627684	28.511623905605816	18.073998583822487
22	22.307839932235243	22.23372223067811	33.474068730932885	21.984369106153753
23	23.693443892238157	25.657298277425205	29.120050823566785	21.52920700676986
24	20.241147236137675	28.72947700696839	28.995241908266113	22.034133848627828
25	20.58208866330049	31.025140459662104	26.951181581751165	21.441589295286246
26	20.71139758190999	25.595754114525082	30.538610690154922	23.154237613410007
27	23.98885587415873	26.23528399653235	27.791226317078177	21.984633812230744
28	18.937205100886104	28.53677098291984	30.81033147818491	21.715692438009146
29	22.236236938409515	24.34515025378695	31.76036158850117	21.658251219302368
30	22.04167797182204	28.891477126086123	29.589904110223607	19.47694079186823
31	22.916266850196212	24.96337129659654	28.120653029891933	23.99970882331531
32	25.87660726221122	27.391255434746643	26.932255097246394	19.79988220579574
33	24.722885825651343	24.607473976083803	28.16591776905718	22.503722429207667
34	22.36819291778891	24.827047666946815	29.325462739310836	23.47929667595344
35	23.637855616070304	25.688533594509995	27.43056428717962	23.243046502240077
36	23.119428764285857	28.31494729040242	27.573240862677107	20.992383082634618
37	21.457736365982623	27.066593431318697	28.75316820085897	22.722502001839707
38	20.64508870962405	26.510445963563207	28.113770671890197	24.73069465492254
39	20.447882682266677	28.98822719722588	28.584418076777997	21.979472043729444
40	20.482559178352336	23.954179378073075	29.941169074389023	25.622092369185566
41	19.937264659753424	25.60726882887414	28.52446215033982	25.931004361032617
42	21.652162979531603	25.104459635632086	28.919403617208545	24.32397376762777
43	22.07185446459887	27.087372858362396	27.670255639893853	23.17051703714488
44	22.62985487489329	24.5714739496132	30.273110494934187	22.525560680559323
45	21.762942472751817	24.082694178451604	28.425594430584137	25.72876891821244
46	23.19711999788235	27.471593729113035	27.455711364493652	21.875574908510963
47	18.76819027072814	27.364123061855196	31.856052835332964	22.0116338320837
48	20.47011799273382	27.09571109978757	30.030375022334578	22.403795885144035
49	22.09210447948859	23.99639999735294	31.544493782716014	22.367001740442458
50	21.5824129282448	25.455327540682017	29.27913917583763	23.683120355235555
51	20.295941394074553	24.855238864146223	29.070683140208192	25.778136601571028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	239.0
2	393.0
3	1434.0
4	2475.0
5	1836.5
6	1198.0
7	1194.0
8	1190.0
9	1207.0
10	1224.0
11	1213.0
12	1202.0
13	1209.0
14	1216.0
15	1186.5
16	1157.0
17	1218.5
18	1280.0
19	1421.0
20	1562.0
21	1924.0
22	2286.0
23	2589.5
24	2893.0
25	3953.0
26	5938.0
27	6863.0
28	8544.5
29	10226.0
30	11966.5
31	13707.0
32	16513.0
33	19319.0
34	21428.0
35	23537.0
36	25175.0
37	26813.0
38	30409.5
39	34006.0
40	36702.5
41	39399.0
42	43325.0
43	47251.0
44	66449.0
45	85647.0
46	78771.5
47	71896.0
48	70045.5
49	68195.0
50	62179.0
51	56163.0
52	53168.0
53	50173.0
54	48163.5
55	46154.0
56	41283.5
57	36413.0
58	32274.5
59	28136.0
60	25222.5
61	22309.0
62	18673.0
63	15037.0
64	12890.0
65	10743.0
66	9102.0
67	7461.0
68	6002.0
69	4543.0
70	4021.5
71	3500.0
72	2837.0
73	2174.0
74	1781.5
75	1036.0
76	683.0
77	529.0
78	375.0
79	256.0
80	137.0
81	100.5
82	64.0
83	58.0
84	52.0
85	33.0
86	14.0
87	7.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	755555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.132442550468205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.84405381814851	23.727898170053393
2	8.119493827895381	5.217983379274591
3	2.833463855593427	2.731383436760518
4	1.6382641249214593	2.1056571150612746
5	1.218792146081699	1.9581384307466028
6	1.0387225535933018	2.0026015667527446
7	0.9436762517393412	2.122583606167889
8	0.8968861917015329	2.305531522332618
9	0.8615114622322615	2.4914220810073204
>10	8.477561997875084	46.21991908642246
>50	0.08574630465779519	1.8347198418898207
>100	0.033461972448193926	1.8324350057948973
>500	0.0029279225892169685	0.5802128443609044
>1k	0.004182746556024241	2.27609555692655
>5k	0.0012548239668072722	2.593418356448462
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	6934	0.9177359689235066	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	6363	0.8421623839429293	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	5999	0.7939858779307927	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATTCAT	3246	0.4296179629543846	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2076	0.2747649079153735	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	1876	0.24829430021639723	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	1645	0.21772074832407964	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTC	1556	0.2059413278980352	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATTCATCGTA	1464	0.19376484835650615	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1360	0.1800001323530385	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1316	0.17417659865926371	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	1288	0.17047071358140706	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1108	0.14664716665232844	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	770	0.10191183964105856	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	766	0.10138242748707904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16080894177128072	0.0
2	0.0	0.0	0.0	0.7886917563909973	0.0
3	0.0	0.0	0.0	1.0005889710213023	0.0
4	0.0	0.0	0.0	1.5187511167287624	0.0
5	0.0	0.0	0.0	2.924869797698381	0.0
6	0.0	0.0	0.0	3.5290614184275135	0.0
7	0.0	0.0	0.0	4.021414721628472	0.0
8	0.0	0.0	0.0	4.663856370482625	0.0
9	0.0	0.0	0.0	4.861062397839999	0.0
10	0.0	0.0	0.0	6.132842744737312	0.0
11	0.0	0.0	0.0	6.940063926517593	0.0
12	0.0	0.0	0.0	8.720741706427726	0.0
13	0.0	0.0	0.0	9.035741938045543	0.0
14	0.0	0.0	0.0	9.19072734612305	0.0
15	0.0	0.0	0.0	9.54053642686502	0.0
16	0.0	0.0	0.0	9.918536704806401	0.0
17	0.0	0.0	0.0	10.355831143993488	0.0
18	0.0	0.0	0.0	10.806890299184044	0.0
19	0.0	0.0	0.0	11.694582128369213	0.0
20	1.3235303849488126E-4	0.0	0.0	12.114405966474976	0.0
21	1.3235303849488126E-4	0.0	0.0	12.668435785614548	0.0
22	1.3235303849488126E-4	0.0	0.0	13.272097994189702	0.0
23	1.3235303849488126E-4	0.0	0.0	13.840157235409732	0.0
24	1.3235303849488126E-4	0.0	0.0	14.323775238070027	0.0
25	1.3235303849488126E-4	0.0	0.0	14.732084361826736	0.0
26	1.3235303849488126E-4	0.0	0.0	15.176393512054053	0.0
27	1.3235303849488126E-4	0.0	0.0	15.562202619266632	0.0
28	1.3235303849488126E-4	0.0	0.0	15.947349961286736	0.0
29	1.3235303849488126E-4	0.0	0.0	16.380806162357473	0.0
30	1.3235303849488126E-4	0.0	0.0	16.866144754518203	0.0
31	1.3235303849488126E-4	0.0	0.0	17.324748032902967	0.0
32	1.3235303849488126E-4	0.0	0.0	17.751189522933473	0.0
33	1.3235303849488126E-4	0.0	0.0	18.190998669851965	0.0
34	1.3235303849488126E-4	0.0	0.0	18.66349901727869	0.0
35	1.3235303849488126E-4	0.0	0.0	19.168955271290642	0.0
36	1.3235303849488126E-4	0.0	0.0	19.64966150710405	0.0
37	1.3235303849488126E-4	0.0	0.0	20.10495595952644	0.0
38	1.3235303849488126E-4	0.0	0.0	20.614779863808725	0.0
39	1.3235303849488126E-4	0.0	0.0	21.31836861644751	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.889533E-5	45.0	1
CGAATCT	25	3.889533E-5	45.0	21
CGGTGAT	25	3.889533E-5	45.0	31
CCGCGTA	25	3.889533E-5	45.0	40
CGATCGA	40	6.8102963E-9	45.0	41
CGCTCGA	85	0.0	42.352943	41
TTTCGCG	240	0.0	42.187496	1
CCGATGA	2325	0.0	41.806454	18
CGATGAA	2345	0.0	41.449894	19
CATCGAA	350	0.0	41.142857	42
GATGAAT	2600	0.0	41.105766	20
CCCATCG	345	0.0	41.086956	40
TCGAATG	275	0.0	40.909092	44
ATCGAAT	330	0.0	40.90909	43
CGTTTTT	1105	0.0	40.723984	1
CGTAAGC	100	0.0	40.5	43
CCGATCG	45	1.9268555E-8	40.0	40
GCTACGA	45	1.9268555E-8	40.0	10
CGATAAA	130	0.0	39.80769	19
TACGGCT	970	0.0	39.43299	7
>>END_MODULE