##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933991.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933290
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40638065338748	33.0	31.0	34.0	31.0	34.0
2	32.627080543025215	34.0	31.0	34.0	31.0	34.0
3	32.69701807583924	34.0	31.0	34.0	31.0	34.0
4	36.07375199562837	37.0	35.0	37.0	35.0	37.0
5	36.098198844946374	37.0	35.0	37.0	35.0	37.0
6	35.64851653826785	37.0	35.0	37.0	35.0	37.0
7	36.03203720172722	37.0	35.0	37.0	35.0	37.0
8	36.0057666963109	37.0	35.0	37.0	35.0	37.0
9	37.87807005325247	39.0	38.0	39.0	35.0	39.0
10	37.57636747420416	39.0	37.0	39.0	35.0	39.0
11	37.57670391839621	39.0	37.0	39.0	35.0	39.0
12	37.36335544150264	39.0	37.0	39.0	35.0	39.0
13	37.26571269380364	39.0	37.0	39.0	34.0	39.0
14	38.48816230753571	40.0	38.0	41.0	34.0	41.0
15	38.52173922360681	40.0	38.0	41.0	34.0	41.0
16	38.61523963612596	40.0	38.0	41.0	35.0	41.0
17	38.54848332243997	40.0	38.0	41.0	34.0	41.0
18	38.50348230453557	40.0	38.0	41.0	34.0	41.0
19	38.536991717472596	40.0	38.0	41.0	34.0	41.0
20	38.46486194001864	40.0	38.0	41.0	34.0	41.0
21	38.412748449035135	40.0	37.0	41.0	34.0	41.0
22	38.392171779404045	40.0	37.0	41.0	34.0	41.0
23	38.34007864650859	40.0	37.0	41.0	34.0	41.0
24	38.31138660009215	40.0	37.0	41.0	34.0	41.0
25	38.236710990153114	40.0	37.0	41.0	34.0	41.0
26	38.15434216588627	40.0	37.0	41.0	34.0	41.0
27	38.086910820859536	40.0	37.0	41.0	34.0	41.0
28	38.01503176933215	40.0	37.0	41.0	34.0	41.0
29	37.988542682338824	40.0	37.0	41.0	34.0	41.0
30	37.91216985074307	40.0	36.0	41.0	34.0	41.0
31	37.87742930921793	40.0	36.0	41.0	34.0	41.0
32	37.78783336369189	40.0	36.0	41.0	33.0	41.0
33	37.72836953144253	40.0	36.0	41.0	33.0	41.0
34	37.71720472736234	40.0	36.0	41.0	33.0	41.0
35	37.61401922232103	40.0	36.0	41.0	33.0	41.0
36	37.50337944261698	40.0	36.0	41.0	33.0	41.0
37	37.3868508180737	40.0	36.0	41.0	33.0	41.0
38	37.39279752274213	40.0	35.0	41.0	33.0	41.0
39	37.39985106451371	40.0	35.0	41.0	33.0	41.0
40	37.34336165607689	40.0	35.0	41.0	33.0	41.0
41	37.2267290981367	39.0	35.0	41.0	32.0	41.0
42	37.20054752542082	39.0	35.0	41.0	32.0	41.0
43	37.04564819080886	39.0	35.0	41.0	32.0	41.0
44	36.96279612982031	39.0	35.0	41.0	32.0	41.0
45	36.98917056863355	39.0	35.0	41.0	32.0	41.0
46	36.894757256586914	39.0	35.0	41.0	32.0	41.0
47	36.788266240932614	39.0	35.0	41.0	31.0	41.0
48	36.729366006278866	39.0	35.0	41.0	31.0	41.0
49	36.73886359009526	39.0	35.0	41.0	31.0	41.0
50	36.65930739641483	39.0	35.0	41.0	31.0	41.0
51	35.728674902763345	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	13.0
10	17.0
11	14.0
12	10.0
13	10.0
14	13.0
15	26.0
16	39.0
17	81.0
18	129.0
19	238.0
20	465.0
21	794.0
22	1229.0
23	1750.0
24	2487.0
25	3451.0
26	4706.0
27	6126.0
28	7200.0
29	8721.0
30	11509.0
31	14826.0
32	19142.0
33	27706.0
34	51436.0
35	71156.0
36	65839.0
37	102989.0
38	200511.0
39	330403.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.868476036387406	4.270483986756528	53.33754781471997	17.523492162136098
2	17.948976202466543	7.648855125416537	53.02564047616497	21.376528195951956
3	19.693771496533767	7.4555604367345625	50.942579476904285	21.908088589827386
4	18.57396950572705	4.689003418015837	52.83138145699622	23.90564561926089
5	20.558133056177606	6.103569094279377	48.57525527970942	24.763042569833598
6	21.82569190712426	8.371460103504806	51.08647901509713	18.716368974273806
7	81.32531153232114	2.2236389546657525	11.554607892509296	4.8964416205038095
8	81.25577258944165	5.088343387371556	7.894009364720504	5.761874658466286
9	75.64326200859325	5.543935968455678	10.041359063099359	8.771442959851708
10	34.68900341801584	34.36038101769011	16.662880776607487	14.28773478768657
11	29.895102272605513	24.6773243043427	25.35685585402179	20.07071756902999
12	27.890902077596458	21.20423448231525	29.45547471846907	21.44938872161922
13	24.971230807144618	24.106547804005185	30.660137792111776	20.26208359673842
14	19.689807026754814	26.3900823966827	29.108101447567208	24.812009128995275
15	17.022468900341803	26.152964244768505	34.81061620718105	22.013950647708644
16	20.417340805108807	25.86034351594895	30.080682317393308	23.641633361548926
17	19.73663062927922	25.847485776125318	29.594874047723646	24.82100954687182
18	20.08550396982717	25.47386128641687	30.768678545789623	23.671956197966335
19	21.344383846392866	26.84996089104137	28.56507623568237	23.24057902688339
20	24.749327647355056	25.992563940468667	30.926293006461016	18.331815405715265
21	25.256672631229304	27.747109687235476	27.86186501516142	19.134352666373797
22	22.287284766792745	24.21905302746199	28.361495355141486	25.132166850603777
23	21.657684106762098	25.852200280727324	28.189737380664106	24.30037823184648
24	22.730448199380685	23.730458914163872	29.36011314811045	24.178979738344992
25	21.09955105058449	27.69653591059585	26.66984538567862	24.53406765314104
26	21.51603467303839	27.09607946083211	27.371877980049074	24.016007886080427
27	21.28234525174383	26.936857782682765	29.07949297645962	22.70130398911378
28	20.22683196005529	25.446645737123507	31.75561722508545	22.570905077735752
29	21.344919585552187	23.588273741280847	30.99101029690664	24.075796376260328
30	21.370849360863183	25.422858918449787	31.763224721147772	21.443066999539266
31	23.06303506948537	25.676584984302842	27.59163818320136	23.668741763010427
32	24.193230399982856	25.474075582080598	28.563361870372557	21.769332147563993
33	22.305928489537013	23.722315678942238	29.747666855961167	24.224088975559578
34	22.474257733394765	23.16128963130431	29.483118859089885	24.881333776211036
35	22.62576476764993	22.433434409454726	30.2697982406326	24.671002582262748
36	24.329200998617793	24.131834692325	29.633768710690138	21.905195598367065
37	21.103194076867855	25.21895659441331	32.5256886926893	21.152160636029528
38	22.183779961212487	24.767435630940007	30.256083318154058	22.792701089693452
39	20.97558100911828	23.255794019008025	32.92417147939011	22.84445349248358
40	23.054998982095597	22.208959701700437	31.695507291409957	23.040534024794006
41	20.40726890891363	23.055534721254915	29.3184326415155	27.21876372831596
42	23.17007575351713	22.24228267741002	30.077360734605534	24.510280834467316
43	22.717269016061458	21.63679027954869	31.47789004489494	24.168050659494906
44	21.052298856732634	22.00109290788501	31.312882383824963	25.633725851557394
45	21.933161182483474	21.384457135509862	28.986702954065724	27.695678727940944
46	23.450160186008638	23.177790397411307	30.052931028940627	23.319118387639428
47	19.440795465503754	21.932411147660428	34.76850710925864	23.85828627757717
48	20.68124591498891	21.58053766782029	32.84241768367817	24.895798733512628
49	21.5790375981742	20.37512455935454	34.803008711118736	23.242829131352526
50	21.7280802322965	20.61577858972024	32.132349001917945	25.523792176065317
51	20.00889327004468	19.96774850260905	29.99592838238918	30.027429844957087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	644.5
2	1150.0
3	2027.5
4	2905.0
5	2100.5
6	1296.0
7	1271.5
8	1247.0
9	1347.0
10	1447.0
11	1449.0
12	1451.0
13	1414.5
14	1378.0
15	1377.0
16	1376.0
17	1317.5
18	1259.0
19	1514.5
20	1770.0
21	2014.0
22	2258.0
23	2832.5
24	3407.0
25	3948.0
26	6445.5
27	8402.0
28	10525.5
29	12649.0
30	14332.0
31	16015.0
32	18287.0
33	20559.0
34	22595.0
35	24631.0
36	27332.5
37	30034.0
38	33143.5
39	36253.0
40	40687.0
41	45121.0
42	50311.0
43	55501.0
44	62518.0
45	69535.0
46	87317.0
47	105099.0
48	106486.5
49	107874.0
50	103266.0
51	98658.0
52	81431.0
53	64204.0
54	55967.5
55	47731.0
56	43028.5
57	38326.0
58	34493.0
59	30660.0
60	29899.5
61	29139.0
62	25788.5
63	22438.0
64	19168.5
65	15899.0
66	13567.0
67	11235.0
68	8757.0
69	6279.0
70	5471.5
71	4664.0
72	3856.0
73	3048.0
74	2420.5
75	1425.5
76	1058.0
77	762.0
78	466.0
79	361.5
80	257.0
81	186.0
82	115.0
83	71.0
84	27.0
85	16.5
86	6.0
87	6.0
88	6.0
89	19.0
90	32.0
91	17.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	933290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.349808644693457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.13199991106734	17.689153515922023
2	13.250487152266215	6.9829560182236765
3	5.568559900438686	4.4019146340921775
4	2.9352895446475626	3.0937727127293058
5	1.7019694477428817	2.2423284633569764
6	1.1019636828022192	1.7421919305144091
7	0.7233212006292923	1.3341562657662243
8	0.5232942555203994	1.1030962798263877
9	0.42344577465506583	1.0041943620208473
>10	5.8728068013528585	39.926061304397486
>50	0.6781412465863058	10.85445901329669
>100	0.07886318425883165	3.8210855518213855
>500	0.0032859660107846514	0.582064809451789
>1k	0.004928949016176977	2.6487303982360784
>5k	0.0016429830053923257	2.5738347403445503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	7230	0.7746788243739888	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC	6210	0.665388035873094	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	5331	0.5712050916649701	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	5010	0.5368106376367474	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	4938	0.5290959937425667	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	4372	0.46845032090775646	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	3729	0.39955426501944735	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1455	0.15590009536157037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1450	0.15536435620225225	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT	1412	0.1512927385914346	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	1383	0.14818545146738957	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1301	0.13939932925457255	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC	1178	0.126220145935347	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	1142	0.12236282398825658	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA	1065	0.11411244093475767	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1048	0.11229092779307612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0714783186362224E-4	0.0	0.0	0.5610260476379261	0.0
2	1.0714783186362224E-4	0.0	0.0	2.3321797083436016	0.0
3	1.0714783186362224E-4	0.0	0.0	3.1462889348434033	0.0
4	1.0714783186362224E-4	0.0	0.0	4.033472982674196	0.0
5	1.0714783186362224E-4	0.0	0.0	5.867093829356363	0.0
6	1.0714783186362224E-4	0.0	0.0	6.623450374481672	0.0
7	1.0714783186362224E-4	0.0	0.0	7.474739898638151	0.0
8	1.0714783186362224E-4	0.0	0.0	8.652616014314951	0.0
9	1.0714783186362224E-4	0.0	0.0	9.113137395664799	0.0
10	1.0714783186362224E-4	0.0	0.0	10.735034126584448	0.0
11	1.0714783186362224E-4	0.0	0.0	12.530081753795711	0.0
12	1.0714783186362224E-4	0.0	0.0	14.96212324143621	0.0
13	1.0714783186362224E-4	0.0	0.0	15.58036623128931	0.0
14	1.0714783186362224E-4	0.0	0.0	15.820484522495688	0.0
15	1.0714783186362224E-4	0.0	0.0	16.305435609510443	0.0
16	1.0714783186362224E-4	0.0	0.0	17.08365031233593	0.0
17	1.0714783186362224E-4	0.0	0.0	18.095768732119705	0.0
18	1.0714783186362224E-4	0.0	0.0	19.15374642394111	0.0
19	1.0714783186362224E-4	0.0	0.0	20.076717847614354	0.0
20	2.1429566372724448E-4	0.0	0.0	20.95715158203774	0.0
21	2.1429566372724448E-4	0.0	0.0	22.09281145196027	0.0
22	2.1429566372724448E-4	0.0	0.0	23.333797640604743	0.0
23	2.1429566372724448E-4	0.0	0.0	24.420705247029325	0.0
24	2.1429566372724448E-4	0.0	0.0	25.27424487565494	0.0
25	3.214434955908667E-4	0.0	0.0	26.0487094043652	0.0
26	3.214434955908667E-4	0.0	0.0	26.728455249708023	0.0
27	3.214434955908667E-4	0.0	0.0	27.389557372306573	0.0
28	3.214434955908667E-4	0.0	0.0	28.070803287295483	0.0
29	3.214434955908667E-4	0.0	0.0	28.802730126755886	0.0
30	3.214434955908667E-4	0.0	0.0	29.642876276398546	0.0
31	3.214434955908667E-4	0.0	0.0	30.396232682231673	0.0
32	3.214434955908667E-4	0.0	0.0	31.115301781868443	0.0
33	3.214434955908667E-4	0.0	0.0	31.82515616796494	0.0
34	3.214434955908667E-4	0.0	0.0	32.5283673884859	0.0
35	3.214434955908667E-4	0.0	0.0	33.29790311693043	0.0
36	3.214434955908667E-4	0.0	0.0	33.993721137052795	0.0
37	3.214434955908667E-4	0.0	0.0	34.69050348766193	0.0
38	3.214434955908667E-4	0.0	0.0	35.40003643026284	0.0
39	3.214434955908667E-4	0.0	0.0	36.127141617289375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	20	7.032603E-4	45.000004	9
GTCGATA	20	7.032603E-4	45.000004	43
CCGATCG	20	7.032603E-4	45.000004	40
GAGTACG	20	7.032603E-4	45.000004	32
CGACATA	20	7.032603E-4	45.000004	36
TCGATAT	20	7.032603E-4	45.000004	44
TTGACGT	20	7.032603E-4	45.000004	20
AATTCGC	20	7.032603E-4	45.000004	28
AGTCGAT	20	7.032603E-4	45.000004	42
AGTCGAC	20	7.032603E-4	45.000004	24
CGAAGTA	20	7.032603E-4	45.000004	45
CCGATAA	25	3.89024E-5	45.0	15
TAACGGG	25	3.89024E-5	45.0	3
GAGCGTT	25	3.89024E-5	45.0	9
TTACGAG	25	3.89024E-5	45.0	1
TATAACG	25	3.89024E-5	45.0	13
CGATAAC	25	3.89024E-5	45.0	16
CGATGAA	120	0.0	44.999996	19
TCGTGCG	105	0.0	42.857143	1
TCGACGT	60	3.6379788E-12	41.249996	26
>>END_MODULE