FastQCFastQC Report
Sat 14 Jan 2017
SRR2933990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940629
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT127110.6549938190143505TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC117630.606143678157958TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC114350.5892419416591218TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC96480.49715839555113317TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.3142280157618999No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG51870.26728447323007126TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG46010.23708807814373586TruSeq Adapter, Index 13 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT31100.1602573186322579No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC29150.15020903016496198No Hit
TGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG27790.1432009930800787TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC26870.1384602621108929No Hit
GCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG25460.13119457660377126TruSeq Adapter, Index 19 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT22260.11470507758051642No Hit
TTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG20070.1034200766864764TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39100.044.021741
CGAACGA603.6379788E-1241.24999622
GCGCGAC2550.040.5882349
GCTACGA1800.040.010
TCTTGCG5000.038.6999971
TTGGGAC40100.038.0985035
CCGATAA659.094947E-1238.0769215
CTTACGG1300.038.076922
TTTCGCG7050.037.9787251
CTCGTTT601.5643309E-1037.49999617
GCGATCG301.1402115E-437.4999969
TTTGGGA102250.037.3202934
TACGGGA2900.037.2413834
GCGATGT5150.037.135929
TGGGCGA22800.037.1052636
TTTCGTG6500.036.6923071
TTGGGCG19200.036.6796845
TTGGGCA27450.036.6393435
TTACGGG3200.036.56253
TTTCTCG6600.036.4772761
Produced by FastQC (version 0.11.5)