##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933990.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1940629
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.315605404227185	33.0	31.0	34.0	31.0	34.0
2	32.53867070934218	34.0	31.0	34.0	31.0	34.0
3	32.5899762396625	34.0	31.0	34.0	31.0	34.0
4	36.00306653152148	37.0	35.0	37.0	35.0	37.0
5	36.05466629634	37.0	35.0	37.0	35.0	37.0
6	35.71346506725397	37.0	35.0	37.0	35.0	37.0
7	36.04132938341125	37.0	35.0	37.0	35.0	37.0
8	36.059562131659376	37.0	35.0	37.0	35.0	37.0
9	37.926270296898586	39.0	38.0	39.0	35.0	39.0
10	37.513516493879045	39.0	37.0	39.0	35.0	39.0
11	37.431427130069686	39.0	37.0	39.0	35.0	39.0
12	37.275618884392635	39.0	37.0	39.0	34.0	39.0
13	37.20746211666424	39.0	37.0	39.0	34.0	39.0
14	38.19872216688507	40.0	38.0	41.0	33.0	41.0
15	38.31675554678406	40.0	38.0	41.0	33.0	41.0
16	38.43462248580229	40.0	38.0	41.0	34.0	41.0
17	38.45311648955055	40.0	38.0	41.0	34.0	41.0
18	38.402895659087854	40.0	38.0	41.0	34.0	41.0
19	38.41575283065439	40.0	37.0	41.0	34.0	41.0
20	38.328664056859914	40.0	37.0	41.0	34.0	41.0
21	38.24898319050163	40.0	37.0	41.0	34.0	41.0
22	38.2941695707938	40.0	37.0	41.0	34.0	41.0
23	38.2295116686394	40.0	37.0	41.0	34.0	41.0
24	38.183595112718606	40.0	37.0	41.0	34.0	41.0
25	38.09040677017606	40.0	37.0	41.0	34.0	41.0
26	38.043526093859256	40.0	37.0	41.0	34.0	41.0
27	38.03765892398805	40.0	37.0	41.0	34.0	41.0
28	37.98205221090688	40.0	37.0	41.0	34.0	41.0
29	37.95600395541858	40.0	37.0	41.0	34.0	41.0
30	37.853431026744424	40.0	36.0	41.0	34.0	41.0
31	37.75406633622398	40.0	36.0	41.0	33.0	41.0
32	37.66466954786309	40.0	36.0	41.0	33.0	41.0
33	37.58376794328024	40.0	36.0	41.0	33.0	41.0
34	37.54782238130009	40.0	36.0	41.0	33.0	41.0
35	37.516594361931105	40.0	36.0	41.0	33.0	41.0
36	37.40842737071331	40.0	36.0	41.0	33.0	41.0
37	37.35014008344717	40.0	36.0	41.0	33.0	41.0
38	37.30769662825816	40.0	36.0	41.0	32.0	41.0
39	37.25298034812424	39.0	35.0	41.0	32.0	41.0
40	37.15674247885608	39.0	35.0	41.0	32.0	41.0
41	37.06234524991639	39.0	35.0	41.0	32.0	41.0
42	37.05756484109018	39.0	35.0	41.0	32.0	41.0
43	36.97425886143101	39.0	35.0	41.0	31.0	41.0
44	36.8741820306715	39.0	35.0	41.0	31.0	41.0
45	36.910938669884864	39.0	35.0	41.0	31.0	41.0
46	36.83065645210908	39.0	35.0	41.0	31.0	41.0
47	36.76665658402508	39.0	35.0	41.0	31.0	41.0
48	36.72665151350413	39.0	35.0	41.0	31.0	41.0
49	36.74055679885233	39.0	35.0	41.0	31.0	41.0
50	36.67756845847403	39.0	35.0	41.0	31.0	41.0
51	35.715943129779056	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	26.0
10	31.0
11	37.0
12	28.0
13	27.0
14	21.0
15	40.0
16	73.0
17	144.0
18	254.0
19	487.0
20	882.0
21	1368.0
22	2295.0
23	3444.0
24	5242.0
25	8590.0
26	12028.0
27	14120.0
28	15894.0
29	18929.0
30	23840.0
31	31770.0
32	42958.0
33	63782.0
34	117906.0
35	127103.0
36	140160.0
37	224195.0
38	442621.0
39	642038.0
40	290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.02320536279732	4.6169051374580095	57.39742114541213	14.96246835433254
2	20.530508407325666	4.258516182124455	52.8827509018983	22.328224508651576
3	20.03448366483238	3.9971060929214186	52.72393641443058	23.24447382781562
4	18.738151393182314	4.661220666083008	51.680151126258544	24.92047681447613
5	18.177199248284964	5.579221994518272	51.18407485408082	25.05950390311595
6	20.87653023839178	5.887884804359824	54.35418104130155	18.88140391594684
7	80.94360127566887	1.8305405103190768	11.35461749772883	5.871240716283226
8	82.30645836994088	2.212478531445217	9.236129110716165	6.244933987897738
9	76.82442136029091	4.410580280929534	12.238145467268602	6.5268528915109485
10	37.94130665882041	25.414337310222614	20.814694617054574	15.829661413902398
11	26.56149114539667	24.768155067248816	27.518861152749956	21.151492634604555
12	24.156961480014985	21.20271314094554	32.96426055675763	21.676064822281848
13	24.201534657062222	22.07341021905784	34.349172355973245	19.375882767906695
14	19.77647453480289	23.972124501901188	34.625938291141686	21.625462672154235
15	17.700704256197348	24.99509179755636	35.93566828074815	21.368535665498143
16	21.026945387294532	25.083465206384115	31.243375214943196	22.646214191378156
17	21.816225563979515	25.457519185789764	29.23005891388823	23.496196336342496
18	21.898518470042443	24.72863179927745	31.993132123656814	21.37971760702329
19	22.13277241554156	26.69897234350306	30.013670825283967	21.154584415671412
20	21.391775553184043	28.541983037458472	30.44049120156403	19.625750207793452
21	21.69008089645161	27.25466846058675	32.2148128261507	18.84043781681094
22	20.269613614967106	24.925372134498662	31.488604983229664	23.316409267304568
23	19.387322357854078	25.583406204895425	30.519743856244546	24.50952758100595
24	20.565548592750083	26.257208358733173	29.23608788696861	23.941155161548135
25	20.363036932870735	26.578289822526614	27.49654879938412	25.562124445218537
26	18.384142461026812	25.88526709638988	30.165837983457937	25.564752459125366
27	18.570164621882903	24.54477388516816	32.01307411153807	24.87198738141087
28	16.453582833194805	23.9994867643429	32.98059546672754	26.566334935734755
29	17.779029376557805	23.945380595672848	33.44415650801879	24.831433519750558
30	20.530508407325666	24.695292093439807	31.406981963064553	23.367217536169974
31	18.6145832098768	26.16630999536748	30.5406649081303	24.678441886625418
32	18.64988104372345	26.00831998285092	28.981685834850452	26.360113138575176
33	19.115194094285926	24.7629505691196	27.963974567008943	28.15788076958553
34	16.233654139972142	23.576891822187548	31.875283735324988	28.31417030251532
35	16.05520684272986	24.07523540048098	30.44404674979092	29.425511006998246
36	18.423253491522594	24.65721165663298	29.106336141529372	27.813198710315056
37	16.30162179375862	25.561557618689612	31.522202337489546	26.61461825006222
38	17.43460496570957	25.426652904805607	31.455935163289837	25.68280696619498
39	17.8289616407876	24.820715345385437	30.34557352281142	27.004749491015538
40	19.552371937139966	23.73483030501966	29.412680115570776	27.300117642269594
41	17.4604728673023	22.881498730566225	27.68406532108919	31.973963081042278
42	17.94510954953265	21.69461550868301	29.43720824536787	30.923066696416473
43	18.416760751282187	21.49164008164363	31.733164865618313	28.35843430145587
44	18.795658521025914	21.9737002796516	30.81006209842273	28.42057910089976
45	18.75304347198769	21.376471236903086	29.879023759822203	29.991461531287023
46	18.896914350965588	21.83065387562486	31.69121970247791	27.58121207093164
47	15.30369792474502	22.761486095487594	34.27914351480886	27.655672464958524
48	15.448084100567394	22.848262084097477	32.896292902971155	28.807360912363983
49	18.64194547231851	21.0198858205252	33.618739078927504	26.719429628228784
50	18.250629048622898	20.36468588277306	30.836496826544384	30.54818824205966
51	16.45543790183492	20.79243379337318	28.45469175200412	34.29743655278778
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	206.0
1	848.0
2	1490.0
3	5538.5
4	9587.0
5	6406.0
6	3225.0
7	3343.5
8	3462.0
9	3778.0
10	4094.0
11	4357.0
12	4620.0
13	4638.5
14	4657.0
15	4509.0
16	4361.0
17	4399.0
18	4437.0
19	4570.5
20	4704.0
21	4970.0
22	5236.0
23	5844.5
24	6453.0
25	8062.5
26	11751.5
27	13831.0
28	16236.0
29	18641.0
30	22308.0
31	25975.0
32	30699.5
33	35424.0
34	41295.5
35	47167.0
36	52862.0
37	58557.0
38	65830.5
39	73104.0
40	83673.5
41	94243.0
42	105603.5
43	116964.0
44	133065.0
45	149166.0
46	178714.5
47	208263.0
48	244345.0
49	280427.0
50	265008.5
51	249590.0
52	202086.5
53	154583.0
54	127256.0
55	99929.0
56	85573.5
57	71218.0
58	63192.5
59	55167.0
60	47056.0
61	38945.0
62	33262.0
63	27579.0
64	23509.5
65	19440.0
66	16254.5
67	13069.0
68	10894.0
69	8719.0
70	7390.0
71	6061.0
72	4823.0
73	3585.0
74	2970.0
75	1837.0
76	1319.0
77	939.0
78	559.0
79	381.0
80	203.0
81	192.0
82	181.0
83	137.0
84	93.0
85	54.0
86	15.0
87	12.0
88	9.0
89	24.5
90	40.0
91	21.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1940629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.638929298248353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.53640580684387	21.367795683805426
2	13.64910362729878	8.636860492971426
3	5.77908989911016	5.485326501785027
4	3.0263190364815147	3.829979761167268
5	1.7532542267363476	2.7735543260783193
6	1.1497806904474734	2.182669798413527
7	0.8345438572819636	1.8482851867782055
8	0.6059670489437219	1.5337718894878891
9	0.5068136241307961	1.4431536379136944
>10	4.78167688862921	33.52235698050067
>50	0.2760273670715329	5.562667129069961
>100	0.08882349007809413	5.0787963909677
>500	0.007580325669435159	1.6050588797609462
>1k	0.003625373146251598	2.16611259439819
>5k	4.943690653979451E-4	1.0913983280830237
>10k+	4.943690653979451E-4	1.8722124188187699
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	12711	0.6549938190143505	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	11763	0.606143678157958	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	11435	0.5892419416591218	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	9648	0.49715839555113317	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6098	0.3142280157618999	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	5187	0.26728447323007126	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	4601	0.23708807814373586	TruSeq Adapter, Index 13 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3110	0.1602573186322579	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	2915	0.15020903016496198	No Hit
TGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	2779	0.1432009930800787	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	2687	0.1384602621108929	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	2546	0.13119457660377126	TruSeq Adapter, Index 19 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	2226	0.11470507758051642	No Hit
TTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	2007	0.1034200766864764	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0305936889534269E-4	0.0	0.0	0.6823045517716163	0.0
2	1.0305936889534269E-4	0.0	0.0	2.4969739192808107	0.0
3	1.0305936889534269E-4	0.0	0.0	3.7295124415846614	0.0
4	1.0305936889534269E-4	0.0	0.0	4.969780416555663	0.0
5	1.0305936889534269E-4	0.0	0.0	6.934658814229819	0.0
6	1.0305936889534269E-4	0.0	0.0	8.357805639305607	0.0
7	1.0305936889534269E-4	0.0	0.0	9.610234619806258	0.0
8	1.5458905334301404E-4	0.0	0.0	11.475351548389723	0.0
9	1.5458905334301404E-4	0.0	0.0	12.391137100393738	0.0
10	1.5458905334301404E-4	0.0	0.0	14.014734397970967	0.0
11	1.5458905334301404E-4	0.0	0.0	17.031385184906544	0.0
12	1.5458905334301404E-4	0.0	0.0	19.593698744067	0.0
13	1.5458905334301404E-4	0.0	0.0	20.48129755867814	0.0
14	1.5458905334301404E-4	0.0	0.0	20.81320025620559	0.0
15	1.5458905334301404E-4	0.0	0.0	21.234249307827515	0.0
16	1.5458905334301404E-4	0.0	0.0	22.20578997840391	0.0
17	1.5458905334301404E-4	0.0	0.0	23.59461803363755	0.0
18	1.5458905334301404E-4	0.0	0.0	25.049146436541967	0.0
19	1.5458905334301404E-4	0.0	0.0	26.13224887394757	0.0
20	1.5458905334301404E-4	0.0	0.0	27.258275538498086	0.0
21	1.5458905334301404E-4	0.0	0.0	28.882182014182	0.0
22	1.5458905334301404E-4	0.0	0.0	30.57596274197696	0.0
23	1.5458905334301404E-4	0.0	0.0	32.21996579459547	0.0
24	2.0611873779068538E-4	0.0	0.0	33.37850769003246	0.0
25	2.0611873779068538E-4	0.0	0.0	34.41193551163051	0.0
26	2.0611873779068538E-4	0.0	0.0	35.327051177736706	0.0
27	2.0611873779068538E-4	0.0	0.0	36.29730360620191	0.0
28	2.0611873779068538E-4	0.0	0.0	37.27626455133877	0.0
29	2.576484222383567E-4	0.0	0.0	38.29052333032228	0.0
30	2.576484222383567E-4	0.0	0.0	39.3599188716648	0.0
31	3.091781066860281E-4	0.0	0.0	40.324915272316346	0.0
32	3.091781066860281E-4	0.0	0.0	41.22240778634144	0.0
33	3.091781066860281E-4	0.0	0.0	42.1250532688113	0.0
34	3.091781066860281E-4	0.0	0.0	43.02682274664554	0.0
35	3.091781066860281E-4	0.0	0.0	43.94616384687645	0.0
36	3.091781066860281E-4	0.0	0.0	44.80511215693468	0.0
37	3.091781066860281E-4	0.0	0.0	45.67689135842039	0.0
38	3.091781066860281E-4	0.0	0.0	46.65054474605914	0.0
39	3.091781066860281E-4	0.0	0.0	48.106103742652515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3910	0.0	44.02174	1
CGAACGA	60	3.6379788E-12	41.249996	22
GCGCGAC	255	0.0	40.588234	9
GCTACGA	180	0.0	40.0	10
TCTTGCG	500	0.0	38.699997	1
TTGGGAC	4010	0.0	38.098503	5
CCGATAA	65	9.094947E-12	38.07692	15
CTTACGG	130	0.0	38.07692	2
TTTCGCG	705	0.0	37.978725	1
CTCGTTT	60	1.5643309E-10	37.499996	17
GCGATCG	30	1.1402115E-4	37.499996	9
TTTGGGA	10225	0.0	37.320293	4
TACGGGA	290	0.0	37.241383	4
GCGATGT	515	0.0	37.13592	9
TGGGCGA	2280	0.0	37.105263	6
TTTCGTG	650	0.0	36.692307	1
TTGGGCG	1920	0.0	36.679684	5
TTGGGCA	2745	0.0	36.639343	5
TTACGGG	320	0.0	36.5625	3
TTTCTCG	660	0.0	36.477276	1
>>END_MODULE