FastQCFastQC Report
Sat 14 Jan 2017
SRR2933989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111049
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA90850.4303547667534008No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT69750.33040445768904464No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC68330.3236779439984577TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT57560.2726606535423858TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC56060.26555518133401923TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC50290.23822279823916925TruSeq Adapter, Index 19 (95% over 22bp)
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG42280.20027957664649187No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTA39860.1888160814836605No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTGA36840.1745103974374825No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31410.14878858804319559No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC22740.10771895867883693No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTC22120.10478203016604541No Hit
GCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG21970.10407148294520877TruSeq Adapter, Index 19 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG21230.10056611665574794TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC351.2125747E-745.09
CTATCGC253.8919196E-545.037
AATTCGC302.166369E-644.99999616
CTCAACT45500.042.5274732
TTGACCC41650.042.2448968
CTTGACC47200.042.2351657
GCATTAG35100.042.1794916
ACTTGAC44700.042.0805366
CATTAGA34250.041.97810417
TGCATTA35100.041.92307715
TCAACTT45250.041.817683
TCTTGCG8450.041.538461
GCTCAAC50000.041.221
CCTGCAT37900.040.72559413
CGGGTAT3150.040.7142876
AACTTGA46300.040.3347745
CCCTGCA38800.040.01288612
TTGTTCG4650.039.677421
ACCCTGC39250.039.6687911
ATTAGAA36400.039.49862718
Produced by FastQC (version 0.11.5)