##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933989.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2111049
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50697496836881	34.0	31.0	34.0	31.0	34.0
2	32.74238257851902	34.0	31.0	34.0	31.0	34.0
3	32.81052405699726	34.0	31.0	34.0	31.0	34.0
4	36.14970708875067	37.0	35.0	37.0	35.0	37.0
5	36.15808870376765	37.0	35.0	37.0	35.0	37.0
6	35.739366542415645	37.0	35.0	37.0	35.0	37.0
7	36.10657781984217	37.0	35.0	37.0	35.0	37.0
8	36.12947165129753	37.0	37.0	37.0	35.0	37.0
9	38.00642713646154	39.0	39.0	39.0	37.0	39.0
10	37.687369170492964	39.0	37.0	39.0	35.0	39.0
11	37.6018590757486	39.0	37.0	39.0	35.0	39.0
12	37.46087229619019	39.0	37.0	39.0	35.0	39.0
13	37.4219830993975	39.0	37.0	39.0	35.0	39.0
14	38.62798921294579	40.0	38.0	41.0	35.0	41.0
15	38.72499264583627	40.0	38.0	41.0	35.0	41.0
16	38.81064674481739	40.0	38.0	41.0	35.0	41.0
17	38.79183050701334	40.0	38.0	41.0	35.0	41.0
18	38.716386024199345	40.0	38.0	41.0	35.0	41.0
19	38.732267701981336	40.0	38.0	41.0	35.0	41.0
20	38.6928233309601	40.0	38.0	41.0	35.0	41.0
21	38.606876960222145	40.0	38.0	41.0	35.0	41.0
22	38.62548382344512	40.0	38.0	41.0	35.0	41.0
23	38.5586786474402	40.0	38.0	41.0	35.0	41.0
24	38.52722745895524	40.0	38.0	41.0	34.0	41.0
25	38.44966459802686	40.0	38.0	41.0	34.0	41.0
26	38.39449297481963	40.0	38.0	41.0	34.0	41.0
27	38.39085213086006	40.0	38.0	41.0	34.0	41.0
28	38.301201440610804	40.0	37.0	41.0	34.0	41.0
29	38.28564424605966	40.0	37.0	41.0	34.0	41.0
30	38.229498699461736	40.0	37.0	41.0	34.0	41.0
31	38.1978632423975	40.0	37.0	41.0	34.0	41.0
32	38.12183042648465	40.0	37.0	41.0	34.0	41.0
33	38.06264847476302	40.0	37.0	41.0	34.0	41.0
34	38.00893299966036	40.0	37.0	41.0	34.0	41.0
35	37.95773286171946	40.0	37.0	41.0	34.0	41.0
36	37.856966370747436	40.0	37.0	41.0	33.0	41.0
37	37.784587662342275	40.0	37.0	41.0	33.0	41.0
38	37.743523243657535	40.0	36.0	41.0	33.0	41.0
39	37.6978336362633	40.0	36.0	41.0	33.0	41.0
40	37.60549660382113	40.0	36.0	41.0	33.0	41.0
41	37.48512564132808	40.0	36.0	41.0	33.0	41.0
42	37.475210191710374	40.0	36.0	41.0	33.0	41.0
43	37.364545777952095	40.0	35.0	41.0	33.0	41.0
44	37.26644099686933	39.0	35.0	41.0	33.0	41.0
45	37.2867550682149	39.0	35.0	41.0	33.0	41.0
46	37.2286005677746	39.0	35.0	41.0	33.0	41.0
47	37.16590093361168	39.0	35.0	41.0	33.0	41.0
48	37.10019521100647	39.0	35.0	41.0	33.0	41.0
49	37.07343221308459	39.0	35.0	41.0	33.0	41.0
50	37.00519410018432	39.0	35.0	41.0	32.0	41.0
51	36.08162861212601	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	33.0
10	49.0
11	30.0
12	28.0
13	26.0
14	28.0
15	40.0
16	63.0
17	137.0
18	237.0
19	441.0
20	810.0
21	1334.0
22	2078.0
23	3011.0
24	4422.0
25	6668.0
26	9164.0
27	11781.0
28	13979.0
29	16756.0
30	21119.0
31	28240.0
32	37908.0
33	57076.0
34	111546.0
35	136020.0
36	140089.0
37	229944.0
38	454930.0
39	822494.0
40	560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.785734485556706	4.438977967825474	54.513798590179576	16.261488956438246
2	18.888855730018584	4.131926828794595	53.01880723753926	23.96041020364757
3	20.08949105397364	4.2170503858508255	53.55891786500455	22.13454069517098
4	18.89359271149083	4.532391242458134	50.57551956397033	25.998496482080707
5	18.476975191006936	7.7868869931489035	49.065796198951325	24.670341616892834
6	21.401255963267552	8.047136755234009	51.628503175435526	18.92310410606291
7	83.74713234984124	2.109898917552364	7.759365130795164	6.383603601811232
8	85.21734928938173	2.6576834550027026	7.987024460351229	4.137942795264345
9	79.92732523025282	5.212574412057702	10.033210977101906	4.8268893805875654
10	39.99580303441559	33.376960932692704	14.403455343765115	12.223780689126592
11	27.83265570813373	26.537707083066287	27.006857728077367	18.622779480722617
12	26.912497057150258	21.436025407273824	29.673967776209835	21.97750975936608
13	25.6373490146368	21.460657710929496	30.192240919088093	22.709752355345614
14	20.234063728506538	23.62991100632908	31.144137345935597	24.99188791922878
15	18.81784837774964	25.05114755744656	34.89568456250897	21.235319502294832
16	23.869223310306868	24.32989475848263	30.959868766665295	20.84101316454521
17	22.91922167604826	24.779671149272232	27.950274958089555	24.35083221658995
18	22.365515911757612	26.2983947790885	30.163913769884072	21.172175539269812
19	23.57264090032965	25.987696164323992	29.65681990328031	20.782843032066047
20	25.672307937901962	26.560776182836115	29.419639240965033	18.347276638296883
21	25.419968934875502	26.816383703078422	28.821974288611962	18.941673073434107
22	25.45109090314815	23.71655039745643	28.782278383874555	22.050080315520862
23	23.38856180031823	25.809396181708717	29.164884377387736	21.63715764058532
24	22.59781748315648	26.08513587320806	28.496685770912944	22.82036087272252
25	22.13070374017846	26.701275053302886	27.12499804599514	24.043023160523514
26	21.258104383176327	27.074691302759906	29.00330593936948	22.663898374694284
27	21.372455115916306	26.2289506307054	30.003993275381102	22.394600977997197
28	20.039610639070908	27.06493312092708	29.618308243910967	23.277147996091045
29	20.558262740466944	25.911714981509192	29.181748031428924	24.34827424659494
30	22.064149150493428	26.187549412637985	28.552013714508757	23.19628772235983
31	22.25860223992906	26.69630122275703	27.365778814229323	23.67931772308459
32	22.46778734174337	27.458102583123367	26.93187131137174	23.142238763761526
33	23.530576504856114	25.842318202940813	26.95219296188767	23.674912330315404
34	20.920783932537805	25.488986754926106	29.444555763509044	24.145673549027048
35	22.387684985047716	25.060716260020495	28.728655753608752	23.82294300132304
36	23.195908763842052	26.485316067983263	27.64350803794701	22.675267130227674
37	22.284560898396958	28.723113485286223	26.869911593714786	22.122414022602033
38	21.55364465722965	27.04584308559394	26.872990631671744	24.52752162550467
39	21.260757092800784	25.84937630533446	27.739242433501072	25.150624168363688
40	22.169594358065588	24.030280680363177	30.172440336534113	23.627684625037126
41	20.246948318111045	25.42802180337832	29.45033488090518	24.874694997605456
42	21.90560238061741	23.533087105036408	29.208369867302935	25.352940647043248
43	22.46930317581449	24.448035076400405	29.01121669842813	24.071445049356978
44	21.85449035053189	24.486262516881418	28.230988480134755	25.428258652451934
45	21.386808169777204	23.98679519044797	27.4079379493323	27.218458690442525
46	21.662784710350163	24.473756885794693	28.92614051118662	24.937317892668524
47	19.709632509714364	24.704732102381328	31.18885445103359	24.39678093687072
48	19.76979217441187	24.351305914737175	30.634011811189605	25.244890099661355
49	21.652126502037614	24.192143337269766	29.847246558464533	24.308483602228087
50	20.357130507155446	23.965762992711205	29.40561777580719	26.271488724326154
51	19.431666436923063	24.039044096086826	27.744216264046926	28.785073202943178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	303.0
1	1317.0
2	2331.0
3	4295.5
4	6260.0
5	4372.0
6	2484.0
7	2562.5
8	2641.0
9	2653.5
10	2666.0
11	2759.0
12	2852.0
13	2828.0
14	2804.0
15	2870.0
16	2936.0
17	3009.5
18	3083.0
19	3452.0
20	3821.0
21	4905.0
22	5989.0
23	7742.5
24	9496.0
25	11621.5
26	17369.5
27	20992.0
28	25738.5
29	30485.0
30	40511.5
31	50538.0
32	50827.5
33	51117.0
34	55938.0
35	60759.0
36	66373.0
37	71987.0
38	80189.5
39	88392.0
40	98863.0
41	109334.0
42	114890.5
43	120447.0
44	130245.5
45	140044.0
46	155939.5
47	171835.0
48	193655.0
49	215475.0
50	209161.0
51	202847.0
52	175480.5
53	148114.0
54	130499.0
55	112884.0
56	106083.0
57	99282.0
58	95597.0
59	91912.0
60	86400.0
61	80888.0
62	71143.5
63	61399.0
64	53415.0
65	45431.0
66	37163.5
67	28896.0
68	24225.0
69	19554.0
70	15367.5
71	11181.0
72	8964.0
73	6747.0
74	5693.0
75	3586.0
76	2533.0
77	1769.0
78	1005.0
79	802.5
80	600.0
81	348.5
82	97.0
83	105.0
84	113.0
85	82.5
86	52.0
87	49.5
88	47.0
89	27.5
90	8.0
91	4.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2111049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.857151212046013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63308921301147	16.13532836292613
2	12.737903886354097	6.077801982825164
3	5.299472817515254	3.792909730547669
4	2.644624981627425	2.5237287234335954
5	1.5812209047977717	1.8861713112704324
6	1.0300917283754545	1.4745032475678626
7	0.677661890549244	1.1316957535432
8	0.5255622099145697	1.0030733690615166
9	0.42939725040770327	0.9219775619712026
>10	6.116442799004056	39.17640318669465
>50	1.2275127856360937	17.804982297532625
>100	0.08814459515894238	3.7834441153823297
>500	0.0046391719523397245	0.7455808914378281
>1k	0.003025546925438951	1.652029016648514
>5k	0.0012102187701755803	1.8903704491573283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	9085	0.4303547667534008	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	6975	0.33040445768904464	No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	6833	0.3236779439984577	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	5756	0.2726606535423858	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	5606	0.26555518133401923	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	5029	0.23822279823916925	TruSeq Adapter, Index 19 (95% over 22bp)
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATG	4228	0.20027957664649187	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTA	3986	0.1888160814836605	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTGA	3684	0.1745103974374825	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3141	0.14878858804319559	No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	2274	0.10771895867883693	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTC	2212	0.10478203016604541	No Hit
GCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	2197	0.10407148294520877	TruSeq Adapter, Index 19 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	2123	0.10056611665574794	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4210944416733103E-4	0.0	0.0	0.28246620518993165	0.0
2	1.4210944416733103E-4	0.0	0.0	1.1663869479107307	0.0
3	1.4210944416733103E-4	0.0	0.0	1.7364826680953402	0.0
4	1.4210944416733103E-4	0.0	0.0	2.3053467730971664	0.0
5	1.4210944416733103E-4	0.0	0.0	3.3217608875966405	0.0
6	1.4210944416733103E-4	0.0	0.0	3.902420076464355	0.0
7	1.4210944416733103E-4	0.0	0.0	4.449825655396914	0.0
8	1.4210944416733103E-4	0.0	0.0	5.390448066340478	0.0
9	1.4210944416733103E-4	0.0	0.0	5.810713062557999	0.0
10	1.4210944416733103E-4	0.0	0.0	6.62973715910905	0.0
11	1.4210944416733103E-4	0.0	0.0	8.21619962397841	0.0
12	1.4210944416733103E-4	0.0	0.0	9.734686404721066	0.0
13	1.4210944416733103E-4	0.0	0.0	10.41520116302369	0.0
14	1.4210944416733103E-4	0.0	0.0	10.58516405824782	0.0
15	1.4210944416733103E-4	0.0	0.0	11.055830537330019	0.0
16	1.4210944416733103E-4	0.0	0.0	11.633221208981885	0.0
17	1.4210944416733103E-4	0.0	0.0	12.433534228717571	0.0
18	1.4210944416733103E-4	0.0	0.0	13.217694141632904	0.0
19	1.894792588897747E-4	0.0	0.0	13.892192933465779	0.0
20	1.894792588897747E-4	0.0	0.0	14.661052396225763	0.0
21	1.894792588897747E-4	0.0	0.0	15.674529582212445	0.0
22	2.368490736122184E-4	0.0	0.0	16.771282902481182	0.0
23	2.368490736122184E-4	0.0	0.0	17.765575313505277	0.0
24	2.368490736122184E-4	0.0	0.0	18.52050805073686	0.0
25	2.368490736122184E-4	0.0	0.0	19.36918565130416	0.0
26	2.368490736122184E-4	0.0	0.0	19.982908970848143	0.0
27	2.368490736122184E-4	0.0	0.0	20.587205697262355	0.0
28	2.368490736122184E-4	0.0	0.0	21.187570729054606	0.0
29	2.8421888833466205E-4	0.0	0.0	21.8445900592549	0.0
30	2.8421888833466205E-4	0.0	0.0	22.60099126074288	0.0
31	2.8421888833466205E-4	0.0	0.0	23.279279637753554	0.0
32	2.8421888833466205E-4	0.0	0.0	24.00730632022279	0.0
33	3.315887030571057E-4	0.0	0.0	24.617429533847865	0.0
34	3.315887030571057E-4	0.0	0.0	25.35379330370825	0.0
35	3.315887030571057E-4	0.0	0.0	26.023034046106936	0.0
36	3.315887030571057E-4	0.0	0.0	26.679769157418896	0.0
37	3.315887030571057E-4	0.0	0.0	27.33929908779948	0.0
38	3.315887030571057E-4	0.0	0.0	28.03350372255689	0.0
39	3.315887030571057E-4	0.0	0.0	28.946414791887825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	35	1.2125747E-7	45.0	9
CTATCGC	25	3.8919196E-5	45.0	37
AATTCGC	30	2.166369E-6	44.999996	16
CTCAACT	4550	0.0	42.527473	2
TTGACCC	4165	0.0	42.244896	8
CTTGACC	4720	0.0	42.235165	7
GCATTAG	3510	0.0	42.17949	16
ACTTGAC	4470	0.0	42.080536	6
CATTAGA	3425	0.0	41.978104	17
TGCATTA	3510	0.0	41.923077	15
TCAACTT	4525	0.0	41.81768	3
TCTTGCG	845	0.0	41.53846	1
GCTCAAC	5000	0.0	41.22	1
CCTGCAT	3790	0.0	40.725594	13
CGGGTAT	315	0.0	40.714287	6
AACTTGA	4630	0.0	40.334774	5
CCCTGCA	3880	0.0	40.012886	12
TTGTTCG	465	0.0	39.67742	1
ACCCTGC	3925	0.0	39.66879	11
ATTAGAA	3640	0.0	39.498627	18
>>END_MODULE