FastQCFastQC Report
Sat 14 Jan 2017
SRR2933988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648072
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC112221.7315977237097113TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT109101.683454924761446TruSeq Adapter, Index 13 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC93081.4362601686232395TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC71611.1049698181683518TruSeq Adapter, Index 13 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG31390.48435976249552526TruSeq Adapter, Index 13 (100% over 23bp)
TCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG28600.44130899035909593TruSeq Adapter, Index 13 (100% over 23bp)
TGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG20030.30907059709415TruSeq Adapter, Index 13 (100% over 23bp)
GCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG18840.29070843980298483TruSeq Adapter, Index 13 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC18710.28870248984680713TruSeq Adapter, Index 13 (100% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.26848868644224716No Hit
TTCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT17140.2644767865298917TruSeq Adapter, Index 13 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG16880.26046488661753636TruSeq Adapter, Index 13 (100% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC15410.23778222172844993No Hit
CGTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT13040.2012122109889025TruSeq Adapter, Index 13 (100% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC10560.1629448579787431TruSeq Adapter, Index 13 (100% over 21bp)
GTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG9880.15245219666950588TruSeq Adapter, Index 13 (95% over 24bp)
TGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT9310.14365687763088053TruSeq Adapter, Index 13 (100% over 22bp)
TTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT8200.12652915108197854TruSeq Adapter, Index 13 (95% over 23bp)
TTGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT8040.12406028959745213TruSeq Adapter, Index 13 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT7920.12220864348405733TruSeq Adapter, Index 13 (100% over 22bp)
ACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC7790.12020269352787961TruSeq Adapter, Index 13 (100% over 24bp)
GTCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT7300.11264180523151748TruSeq Adapter, Index 13 (95% over 23bp)
GTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC7200.11109876680368848TruSeq Adapter, Index 13 (100% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC7100.10955572837585947No Hit
GGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG6870.10600673999185276TruSeq Adapter, Index 13 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTG207.031006E-445.0000041
ATCTCGT207.031006E-445.00000411
GTCGAGG207.031006E-445.0000042
CACGGTA207.031006E-445.00000445
CACGGGC207.031006E-445.0000044
ACCATCG207.031006E-445.0000041
TCGGGTA600.045.0000045
CCAGATA207.031006E-445.00000444
TACGCGG453.8380676E-1045.0000042
CTACGAA406.8066583E-945.00000411
GCGATCG207.031006E-445.0000049
TCCGTAA453.8380676E-1045.00000421
CGTGTAA302.1640299E-645.00000417
ATGCGCC207.031006E-445.00000410
GAACCGC207.031006E-445.0000049
GTCTTCG207.031006E-445.0000041
GGTCTCG207.031006E-445.0000041
CGACTGA207.031006E-445.0000042
TGTGTCG207.031006E-445.0000041
ATGCACG207.031006E-445.00000435
Produced by FastQC (version 0.11.5)