##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933988.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648072
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.144105901813376	33.0	31.0	34.0	30.0	34.0
2	32.38190818304139	34.0	31.0	34.0	30.0	34.0
3	32.44859676085373	34.0	31.0	34.0	30.0	34.0
4	35.894636707032554	37.0	35.0	37.0	35.0	37.0
5	35.953841548469924	37.0	35.0	37.0	35.0	37.0
6	35.597263884259775	37.0	35.0	37.0	33.0	37.0
7	35.91204680961375	37.0	35.0	37.0	35.0	37.0
8	35.90139675838487	37.0	35.0	37.0	35.0	37.0
9	37.714898961843744	39.0	38.0	39.0	35.0	39.0
10	37.43772914120653	39.0	37.0	39.0	35.0	39.0
11	37.436496253502696	39.0	37.0	39.0	35.0	39.0
12	37.22860577219815	39.0	37.0	39.0	34.0	39.0
13	37.07485279413398	39.0	37.0	39.0	33.0	39.0
14	37.96791714500858	40.0	37.0	41.0	33.0	41.0
15	38.07265396437433	40.0	37.0	41.0	33.0	41.0
16	38.206086669382415	40.0	37.0	41.0	33.0	41.0
17	38.23148971101976	40.0	37.0	41.0	34.0	41.0
18	38.231827636435455	40.0	37.0	41.0	34.0	41.0
19	38.2818544853041	40.0	37.0	41.0	34.0	41.0
20	38.19836684812799	40.0	37.0	41.0	34.0	41.0
21	38.113015220531054	40.0	37.0	41.0	34.0	41.0
22	38.13148693355059	40.0	37.0	41.0	34.0	41.0
23	38.09264094113	40.0	37.0	41.0	34.0	41.0
24	38.04772000641904	40.0	37.0	41.0	34.0	41.0
25	37.91227517930106	40.0	36.0	41.0	34.0	41.0
26	37.83926940216519	40.0	36.0	41.0	34.0	41.0
27	37.81292819316372	40.0	36.0	41.0	34.0	41.0
28	37.66760946314607	39.0	36.0	41.0	33.0	41.0
29	37.56135275092891	39.0	36.0	41.0	33.0	41.0
30	37.48689343159402	39.0	36.0	41.0	33.0	41.0
31	37.415199237121804	39.0	36.0	41.0	33.0	41.0
32	37.36065282869804	39.0	35.0	41.0	33.0	41.0
33	37.36719994074733	39.0	35.0	41.0	33.0	41.0
34	37.38215506918984	39.0	36.0	41.0	33.0	41.0
35	37.3555824044242	39.0	35.0	41.0	33.0	41.0
36	37.24329704106951	39.0	35.0	41.0	32.0	41.0
37	37.107690194916614	39.0	35.0	41.0	32.0	41.0
38	37.01828346233135	39.0	35.0	41.0	32.0	41.0
39	37.00689892481083	39.0	35.0	41.0	32.0	41.0
40	36.96518442395289	39.0	35.0	41.0	31.0	41.0
41	36.96389444382723	39.0	35.0	41.0	32.0	41.0
42	37.002831475515066	39.0	35.0	41.0	31.0	41.0
43	36.86540384401733	39.0	35.0	41.0	31.0	41.0
44	36.66744898714958	39.0	35.0	41.0	31.0	41.0
45	36.706313496031306	39.0	35.0	40.0	31.0	41.0
46	36.63119221321088	39.0	35.0	40.0	31.0	41.0
47	36.552565147082426	39.0	35.0	40.0	31.0	41.0
48	36.559778851732524	39.0	35.0	40.0	31.0	41.0
49	36.611749929020235	39.0	35.0	40.0	31.0	41.0
50	36.567781049019246	39.0	35.0	40.0	31.0	41.0
51	35.58260193311854	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	20.0
11	28.0
12	19.0
13	14.0
14	16.0
15	21.0
16	27.0
17	45.0
18	82.0
19	150.0
20	307.0
21	505.0
22	822.0
23	1136.0
24	1808.0
25	2639.0
26	3717.0
27	4554.0
28	5602.0
29	6843.0
30	8831.0
31	11549.0
32	15714.0
33	23290.0
34	42618.0
35	47652.0
36	50944.0
37	81858.0
38	156567.0
39	180640.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.945752941031245	4.750552407757163	53.160914219407715	19.142780431803875
2	20.830864471848805	4.339486970583516	49.70774852176918	25.12190003579849
3	20.690293671073583	4.852392943993877	51.645496179436854	22.811817205495686
4	21.45872680813243	4.7030268241800295	48.52470095915269	25.313545408534853
5	18.52139885691713	5.143101383796862	49.92639706699256	26.40910269229345
6	20.589687565579133	6.089138243898827	52.162105445074005	21.159068745448035
7	72.76552605266082	2.3619289214778605	16.121819797800242	8.750725228061079
8	70.06891209618684	3.0237381031737214	14.948339073436284	11.95901072720315
9	63.072467256724565	6.223999802491082	20.24636151538718	10.457171425397178
10	31.02371341455888	27.962170869903346	25.48451406633831	15.52960164919947
11	25.310767939364766	23.077682726610625	30.549537705687023	21.062011628337594
12	24.10488340801639	19.603840314039182	36.2393376044637	20.051938673480723
13	22.356003653914996	23.61620313792295	35.126035378785076	18.901757829376983
14	16.26516806774556	26.758755200039502	33.87818020219975	23.097896530015184
15	12.803978570282315	26.145860336505823	38.671474774407784	22.378686318804082
16	15.693163722549347	26.823871421693884	31.28680146650372	26.196163389253048
17	16.1141046056611	26.808441037415598	29.356306089446853	27.721148267476455
18	17.434173980668817	23.571300719673122	34.73564665654433	24.25887864311373
19	17.865761828932587	24.584768359071212	32.707785554691455	24.841684257304745
20	19.562023972645015	27.093903146563964	33.23905368539298	20.10501919539804
21	20.558826797022554	26.663241121356883	33.57744201261588	19.200490069004676
22	16.63071387129825	26.141076917379554	30.88021084077078	26.347998370551416
23	18.1580133071634	25.109864336061428	30.12026441506499	26.61185794171018
24	21.93213099779037	23.61867199940747	27.63998444617265	26.809212556629507
25	17.240985569504623	26.865687763088054	25.87181671172339	30.021509955683932
26	15.390882494537644	29.028256119690404	26.690707205372245	28.89015418039971
27	16.24927477193892	26.237053907590514	28.02080633016085	29.49286499030972
28	11.95469021960523	25.999271685862062	28.844171635250405	33.2018664592823
29	13.873304200767816	31.111512301102344	26.537637793331605	28.477545704798235
30	17.585700354281624	29.846220790282562	24.44033996222642	28.127738893209397
31	15.503678603611945	29.913651569578686	23.545531977928377	31.03713784888099
32	15.786517547433002	31.27322272833883	24.37692108284265	28.563338641385528
33	16.127683343825993	26.736998358207114	23.849356244367907	33.285962053598986
34	13.314878593736498	24.038069844091396	28.51195546173882	34.13509610043329
35	13.003493439000605	27.31347751484403	26.243534668987394	33.43949437716797
36	17.22663531212581	26.308033675270647	25.420940883111754	31.044390129491784
37	14.362601686232393	25.74281869915688	28.795103013245445	31.09947660136528
38	14.539588193904383	29.98987766791344	26.904880939154907	28.56565319902727
39	17.812372699329703	27.241263316421634	27.795831327383375	27.150532656865284
40	20.150075917490646	24.37229196755916	28.642805120418718	26.834826994531475
41	15.086595316569765	22.72000641903986	26.784061030255895	35.40933723413448
42	17.52598476712464	20.406065992667482	28.161222827093287	33.90672641311459
43	20.08480539199348	19.8229517707909	27.80539816563592	32.2868446715797
44	17.152723771432804	21.860071103210753	27.616838869755213	33.37036625560123
45	17.570269970003334	21.604389635719485	26.052043600093818	34.77329679418336
46	18.869816933920923	21.76054512461578	31.144224715772324	28.22541322569097
47	12.726672345048081	22.135040551049883	35.18760261205545	29.950684491846584
48	12.66649384636275	22.085046105988223	32.84372724018319	32.404732807465834
49	17.57798516214248	18.973354812428248	34.73765260650051	28.711007418928762
50	18.626325470009505	18.445481366267945	30.358509548321795	32.569683615400756
51	15.132423557876285	17.845393721685245	27.42627362391833	39.59590909652014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	388.0
2	548.0
3	1738.5
4	2929.0
5	2009.0
6	1089.0
7	1142.5
8	1196.0
9	1411.5
10	1627.0
11	1661.0
12	1695.0
13	1703.5
14	1712.0
15	1574.0
16	1436.0
17	1421.0
18	1406.0
19	1413.5
20	1421.0
21	1560.5
22	1700.0
23	1901.0
24	2102.0
25	2240.0
26	2916.0
27	3454.0
28	3999.0
29	4544.0
30	5217.0
31	5890.0
32	7112.0
33	8334.0
34	9084.5
35	9835.0
36	11418.0
37	13001.0
38	14856.5
39	16712.0
40	20308.5
41	23905.0
42	29802.5
43	35700.0
44	44631.5
45	53563.0
46	66435.0
47	79307.0
48	100403.5
49	121500.0
50	112589.5
51	103679.0
52	78764.5
53	53850.0
54	42546.5
55	31243.0
56	25451.0
57	19659.0
58	16630.0
59	13601.0
60	11825.0
61	10049.0
62	8331.0
63	6613.0
64	5496.5
65	4380.0
66	3692.0
67	3004.0
68	2408.0
69	1812.0
70	1542.5
71	1273.0
72	1017.5
73	762.0
74	637.0
75	354.0
76	196.0
77	170.5
78	145.0
79	98.5
80	52.0
81	36.5
82	21.0
83	14.0
84	7.0
85	4.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.296487200114125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.64926448194906	20.232948783561117
2	14.123803073876747	8.840508442370323
3	6.4376798453579065	6.044302946360293
4	3.5972850206688065	4.503295384180943
5	2.241347447317918	3.5073150847996843
6	1.4563768077153534	2.7347686872723984
7	1.07090371821198	2.3460867156703107
8	0.7698412312292094	1.9274660991428043
9	0.6378193678150902	1.7965355112728867
>10	4.726935404098068	27.13958062380339
>50	0.17872233281471622	3.8248444320436157
>100	0.09259708608052986	5.761182637240949
>500	0.00995667592263762	2.086222124906193
>1k	0.005476171757450691	3.240733397912533
>5k	9.95667592263762E-4	2.5659441504914184
>10k+	9.95667592263762E-4	3.448264978971163
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	11222	1.7315977237097113	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	10910	1.683454924761446	TruSeq Adapter, Index 13 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	9308	1.4362601686232395	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	7161	1.1049698181683518	TruSeq Adapter, Index 13 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	3139	0.48435976249552526	TruSeq Adapter, Index 13 (100% over 23bp)
TCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	2860	0.44130899035909593	TruSeq Adapter, Index 13 (100% over 23bp)
TGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	2003	0.30907059709415	TruSeq Adapter, Index 13 (100% over 23bp)
GCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	1884	0.29070843980298483	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	1871	0.28870248984680713	TruSeq Adapter, Index 13 (100% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1740	0.26848868644224716	No Hit
TTCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	1714	0.2644767865298917	TruSeq Adapter, Index 13 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	1688	0.26046488661753636	TruSeq Adapter, Index 13 (100% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	1541	0.23778222172844993	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	1304	0.2012122109889025	TruSeq Adapter, Index 13 (100% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	1056	0.1629448579787431	TruSeq Adapter, Index 13 (100% over 21bp)
GTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	988	0.15245219666950588	TruSeq Adapter, Index 13 (95% over 24bp)
TGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	931	0.14365687763088053	TruSeq Adapter, Index 13 (100% over 22bp)
TTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	820	0.12652915108197854	TruSeq Adapter, Index 13 (95% over 23bp)
TTGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	804	0.12406028959745213	TruSeq Adapter, Index 13 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	792	0.12220864348405733	TruSeq Adapter, Index 13 (100% over 22bp)
ACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	779	0.12020269352787961	TruSeq Adapter, Index 13 (100% over 24bp)
GTCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	730	0.11264180523151748	TruSeq Adapter, Index 13 (95% over 23bp)
GTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	720	0.11109876680368848	TruSeq Adapter, Index 13 (100% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC	710	0.10955572837585947	No Hit
GGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	687	0.10600673999185276	TruSeq Adapter, Index 13 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9508634843042132	0.0
2	0.0	0.0	0.0	7.000302435531855	0.0
3	0.0	0.0	0.0	9.740430075670604	0.0
4	0.0	0.0	0.0	12.304034119665717	0.0
5	0.0	0.0	0.0	16.1799923465294	0.0
6	0.0	0.0	0.0	18.155698749521658	0.0
7	0.0	0.0	0.0	20.000401189991237	0.0
8	0.0	0.0	0.0	22.747472503055217	0.0
9	0.0	0.0	0.0	23.976965522349367	0.0
10	0.0	0.0	0.0	26.341826216840104	0.0
11	0.0	0.0	0.0	30.683010529694233	0.0
12	0.0	0.0	0.0	34.64707625078695	0.0
13	0.0	0.0	0.0	36.00170968657803	0.0
14	0.0	0.0	0.0	36.518164648372405	0.0
15	0.0	0.0	0.0	37.21592662543668	0.0
16	0.0	0.0	0.0	38.71390833117308	0.0
17	0.0	0.0	0.0	40.825865027342644	0.0
18	0.0	0.0	0.0	43.264637262526385	0.0
19	0.0	0.0	0.0	44.69642261970892	0.0
20	0.0	0.0	0.0	46.358892221851896	0.0
21	0.0	0.0	0.0	48.392616869730524	0.0
22	0.0	0.0	0.0	50.27126615561234	0.0
23	1.5430384278290065E-4	0.0	0.0	51.872168524484934	0.0
24	1.5430384278290065E-4	0.0	0.0	53.05351874483082	0.0
25	1.5430384278290065E-4	0.0	0.0	54.100778925798366	0.0
26	1.5430384278290065E-4	0.0	0.0	55.03323704773544	0.0
27	1.5430384278290065E-4	0.0	0.0	56.02541075682949	0.0
28	1.5430384278290065E-4	0.0	0.0	56.996444839462285	0.0
29	1.5430384278290065E-4	0.0	0.0	57.956369045414704	0.0
30	1.5430384278290065E-4	0.0	0.0	59.00254909948277	0.0
31	1.5430384278290065E-4	0.0	0.0	59.919885444827116	0.0
32	1.5430384278290065E-4	0.0	0.0	60.728437581009516	0.0
33	1.5430384278290065E-4	0.0	0.0	61.5317433865373	0.0
34	1.5430384278290065E-4	0.0	0.0	62.34091273809083	0.0
35	1.5430384278290065E-4	0.0	0.0	63.21442679208483	0.0
36	1.5430384278290065E-4	0.0	0.0	63.95971435272624	0.0
37	1.5430384278290065E-4	0.0	0.0	64.72212963991656	0.0
38	1.5430384278290065E-4	0.0	0.0	65.53083607994174	0.0
39	1.5430384278290065E-4	0.0	0.0	66.70709427347579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTG	20	7.031006E-4	45.000004	1
ATCTCGT	20	7.031006E-4	45.000004	11
GTCGAGG	20	7.031006E-4	45.000004	2
CACGGTA	20	7.031006E-4	45.000004	45
CACGGGC	20	7.031006E-4	45.000004	4
ACCATCG	20	7.031006E-4	45.000004	1
TCGGGTA	60	0.0	45.000004	5
CCAGATA	20	7.031006E-4	45.000004	44
TACGCGG	45	3.8380676E-10	45.000004	2
CTACGAA	40	6.8066583E-9	45.000004	11
GCGATCG	20	7.031006E-4	45.000004	9
TCCGTAA	45	3.8380676E-10	45.000004	21
CGTGTAA	30	2.1640299E-6	45.000004	17
ATGCGCC	20	7.031006E-4	45.000004	10
GAACCGC	20	7.031006E-4	45.000004	9
GTCTTCG	20	7.031006E-4	45.000004	1
GGTCTCG	20	7.031006E-4	45.000004	1
CGACTGA	20	7.031006E-4	45.000004	2
TGTGTCG	20	7.031006E-4	45.000004	1
ATGCACG	20	7.031006E-4	45.000004	35
>>END_MODULE