##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933985.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	649133
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.444634612629464	33.0	31.0	34.0	31.0	34.0
2	32.6694298394936	34.0	31.0	34.0	31.0	34.0
3	32.73251398403717	34.0	31.0	34.0	31.0	34.0
4	36.091904124424424	37.0	35.0	37.0	35.0	37.0
5	36.138142722677784	37.0	35.0	37.0	35.0	37.0
6	35.79134168190494	37.0	35.0	37.0	35.0	37.0
7	36.09270981447562	37.0	35.0	37.0	35.0	37.0
8	36.10015512999647	37.0	37.0	37.0	35.0	37.0
9	37.93652456430346	39.0	38.0	39.0	35.0	39.0
10	37.638254410113184	39.0	37.0	39.0	35.0	39.0
11	37.5987447872778	39.0	37.0	39.0	35.0	39.0
12	37.30132345759652	39.0	37.0	39.0	35.0	39.0
13	37.190574196659234	39.0	37.0	39.0	34.0	39.0
14	38.37521278382088	40.0	37.0	41.0	34.0	41.0
15	38.43384175507947	40.0	37.0	41.0	35.0	41.0
16	38.497731589674224	40.0	38.0	41.0	35.0	41.0
17	38.44926540477837	40.0	37.0	41.0	34.0	41.0
18	38.407625247830566	40.0	37.0	41.0	34.0	41.0
19	38.437269403958815	40.0	37.0	41.0	34.0	41.0
20	38.40042333389305	40.0	37.0	41.0	34.0	41.0
21	38.32241466694807	40.0	37.0	41.0	34.0	41.0
22	38.29186314668951	40.0	37.0	41.0	34.0	41.0
23	38.277468561912585	40.0	37.0	41.0	34.0	41.0
24	38.25572109259581	40.0	37.0	41.0	34.0	41.0
25	38.178268552053275	40.0	36.0	41.0	34.0	41.0
26	38.120175680484586	40.0	36.0	41.0	34.0	41.0
27	38.11966576957265	40.0	36.0	41.0	34.0	41.0
28	38.06037129525074	40.0	36.0	41.0	34.0	41.0
29	38.06710335170143	40.0	36.0	41.0	34.0	41.0
30	37.99296138079561	40.0	36.0	41.0	34.0	41.0
31	37.904860791240004	40.0	36.0	41.0	34.0	41.0
32	37.84131449179136	40.0	36.0	41.0	34.0	41.0
33	37.78437084541997	40.0	36.0	41.0	34.0	41.0
34	37.7341114994924	40.0	36.0	41.0	33.0	41.0
35	37.69008354220168	40.0	36.0	41.0	33.0	41.0
36	37.63542602209409	40.0	36.0	41.0	33.0	41.0
37	37.580326373793966	40.0	35.0	41.0	33.0	41.0
38	37.57424595575945	40.0	35.0	41.0	33.0	41.0
39	37.542466643969725	40.0	35.0	41.0	33.0	41.0
40	37.42299806048992	40.0	35.0	41.0	33.0	41.0
41	37.34055270645615	39.0	35.0	41.0	33.0	41.0
42	37.35486410335016	39.0	35.0	41.0	33.0	41.0
43	37.27478652294676	39.0	35.0	41.0	33.0	41.0
44	37.157067966040856	39.0	35.0	41.0	33.0	41.0
45	37.177251811262096	39.0	35.0	41.0	33.0	41.0
46	37.095949520360236	39.0	35.0	41.0	33.0	41.0
47	37.04244122544995	39.0	35.0	41.0	33.0	41.0
48	37.012878716688256	39.0	35.0	41.0	33.0	41.0
49	37.0105140240906	39.0	35.0	41.0	33.0	41.0
50	36.93931906096285	39.0	35.0	41.0	32.0	41.0
51	36.05023315714961	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	16.0
11	11.0
12	10.0
13	10.0
14	9.0
15	14.0
16	19.0
17	40.0
18	66.0
19	137.0
20	230.0
21	389.0
22	592.0
23	959.0
24	1321.0
25	2106.0
26	2967.0
27	3677.0
28	4558.0
29	5520.0
30	7043.0
31	9164.0
32	12561.0
33	18673.0
34	40869.0
35	56435.0
36	42767.0
37	67388.0
38	135425.0
39	236010.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.700124627772738	4.670383419114419	55.47014248235723	17.159349470755608
2	20.220663562012717	4.489680851227715	53.23084791560435	22.05880767115522
3	21.161148793852725	4.271697787664469	52.00644552040953	22.560707898073275
4	19.90054426442655	4.329775254069659	50.94087036092757	24.828810120576215
5	18.582632526770322	5.843024464940159	49.84818211368086	25.726160894608658
6	22.41004539901684	6.284228347657568	52.519437465049535	18.786288788276053
7	83.21407169254991	2.42354032224521	9.1575994441817	5.20478854102318
8	83.53157211234061	2.9742749174668366	7.793934370922445	5.700218599270103
9	76.88670888708478	5.996305841792052	10.456716882364631	6.660268388758545
10	36.910155545935886	33.31690115893045	16.467503577849225	13.30543971728444
11	32.7415183021045	23.66125277870637	25.43716002729795	18.160068891891186
12	31.35998323918211	20.498572711601472	28.53837349202706	19.60307055718936
13	25.779000605422926	23.388273281438472	29.21589258287593	21.616833530262674
14	20.318640401889905	26.76493106959591	28.861111667408686	24.055316861105506
15	18.191649477071724	26.001451166401957	35.31202388416549	20.494875472360828
16	21.572004504469806	23.592853852754367	33.13450402305845	21.700637619717376
17	20.938698232873694	24.532876929689294	28.930897058075928	25.597527779361084
18	21.594341991548728	24.370506506370806	31.286500609274214	22.748650892806253
19	24.274686389383994	25.567487710530816	28.559170462755706	21.598655437329484
20	27.769655833242187	26.116373686132118	28.290966566173648	17.823003914452045
21	26.371945348641958	26.78511183378445	27.70618655961105	19.136756257962546
22	23.199868131800415	25.61385725267395	27.43767455975894	23.74860005576669
23	23.383959835657716	25.046485080869406	28.005200783198514	23.564354300274363
24	24.61976205184454	21.975465736605596	29.27381599764609	24.130956213903776
25	21.74500449060516	25.21070412380822	26.985995165859695	26.058296219726927
26	19.367525607233034	26.556653259039365	29.852125835537556	24.223695298190048
27	19.967094570758228	26.802057513637422	28.82490953317733	24.405938382427024
28	17.8461116597061	25.76621431971568	32.57498848464028	23.812685535937934
29	21.54073202255932	23.77309426573599	30.297026957495614	24.389146754209076
30	22.146925206390677	24.326909893658154	28.197303172077216	25.328861727873946
31	21.993335726268732	27.524251578644133	26.597630993956557	23.884781701130585
32	23.095575174887117	25.649289128730167	26.958573974824883	24.296561721557833
33	22.7979474160149	24.90183059557903	27.51654899689278	24.783672991513296
34	20.833480966150233	24.1686988644854	30.578633346325024	24.419186823039347
35	19.507096388567522	23.568513694420094	29.64846957403182	27.27592034298056
36	19.750960126815308	25.41127935261341	29.82686136739312	25.010899153178162
37	20.463140835545257	27.040837547929314	29.859058159113772	22.636963457411653
38	21.08458513124429	25.67701842303503	27.794150043211484	25.44424640250919
39	22.547767560731007	23.91405151178572	29.065846290359605	24.472334637123673
40	22.11981211862592	23.840876985147883	28.627261285437655	25.412049610788547
41	17.429556038593017	25.160021135884325	28.50186325452565	28.908559570997006
42	18.757018977620916	22.597218135574682	29.8022131057888	28.843549781015597
43	20.971819334404508	22.198378452489706	30.053163219247825	26.776638993857958
44	19.885139100923848	22.5853561596776	29.69268239328458	27.83682234611397
45	20.540320704693798	23.08186457936971	26.995238263961312	29.382576451975172
46	20.410301124730985	24.14482086105621	29.27658892707658	26.168289087136227
47	17.922983425584587	23.261796889081282	32.79543637436396	26.019783310970173
48	18.777507845079512	21.10676856668818	32.796206632539096	27.319516955693206
49	20.142559383054014	20.757225406811855	33.074577937032934	26.0256372731012
50	19.972332326349147	20.864599396425692	30.00093971497366	29.162128562251493
51	17.748596974733992	21.33399472835305	28.673476775945762	32.243931520967195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	415.5
2	693.0
3	1284.0
4	1875.0
5	1326.0
6	777.0
7	796.5
8	816.0
9	889.5
10	963.0
11	1078.0
12	1193.0
13	1075.0
14	957.0
15	1095.5
16	1234.0
17	1160.0
18	1086.0
19	1121.5
20	1157.0
21	1405.5
22	1654.0
23	1808.5
24	1963.0
25	2722.0
26	4218.5
27	4956.0
28	6075.5
29	7195.0
30	8774.0
31	10353.0
32	11746.5
33	13140.0
34	14535.0
35	15930.0
36	16925.5
37	17921.0
38	20044.5
39	22168.0
40	25196.0
41	28224.0
42	32531.5
43	36839.0
44	40712.5
45	44586.0
46	51665.5
47	58745.0
48	67072.5
49	75400.0
50	74351.0
51	73302.0
52	61390.5
53	49479.0
54	43191.0
55	36903.0
56	33736.5
57	30570.0
58	30023.5
59	29477.0
60	29857.5
61	30238.0
62	24176.0
63	18114.0
64	15101.5
65	12089.0
66	9317.0
67	6545.0
68	5089.0
69	3633.0
70	2911.5
71	2190.0
72	1777.5
73	1365.0
74	1156.0
75	707.5
76	468.0
77	378.5
78	289.0
79	166.5
80	44.0
81	33.5
82	23.0
83	13.5
84	4.0
85	4.5
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	649133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.274047700493547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.8636723022233	17.305052677982907
2	13.672124892387705	7.184441231793995
3	5.946837809106608	4.687425007906967
4	3.1835187585916787	3.3457569487461507
5	1.903729712454951	2.500934263694412
6	1.146336553868624	1.807134077829817
7	0.8057161660731609	1.4818597486325504
8	0.5719617279488534	1.202219977838791
9	0.44739662025693905	1.057942812750337
>10	5.477627964236788	37.49895483811236
>50	0.8783996911191628	14.20425987938605
>100	0.0955556420744492	4.4151283370483085
>500	0.004154593133671705	0.8250783749981493
>1k	0.0029675665240512174	2.483811823279252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	4340	0.6685840960173031	No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	3659	0.5636749325638968	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	3345	0.5153027191654099	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	3292	0.5071379825089773	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	1292	0.19903471245492063	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	920	0.1417275042248661	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	918	0.14141940095481204	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	891	0.1372600068090823	No Hit
TGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	780	0.12016027532108212	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	754	0.11615493281037939	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5405163502702835E-4	0.0	0.0	0.5857043163727618	0.0
2	1.5405163502702835E-4	0.0	0.0	2.397813699195696	0.0
3	1.5405163502702835E-4	0.0	0.0	3.3717281358365696	0.0
4	1.5405163502702835E-4	0.0	0.0	4.337015680915929	0.0
5	1.5405163502702835E-4	0.0	0.0	5.875837463200916	0.0
6	1.5405163502702835E-4	0.0	0.0	6.75069669851941	0.0
7	1.5405163502702835E-4	0.0	0.0	7.530660126661254	0.0
8	1.5405163502702835E-4	0.0	0.0	8.766462342848076	0.0
9	1.5405163502702835E-4	0.0	0.0	9.354939588651325	0.0
10	1.5405163502702835E-4	0.0	0.0	10.553461309161605	0.0
11	1.5405163502702835E-4	0.0	0.0	12.611899256392757	0.0
12	3.081032700540567E-4	0.0	0.0	14.5272232346838	0.0
13	3.081032700540567E-4	0.0	0.0	15.151902614718402	0.0
14	3.081032700540567E-4	0.0	0.0	15.392839371900674	0.0
15	3.081032700540567E-4	0.0	0.0	15.772422600607271	0.0
16	3.081032700540567E-4	0.0	0.0	16.632800982233224	0.0
17	3.081032700540567E-4	0.0	0.0	17.79958806592794	0.0
18	3.081032700540567E-4	0.0	0.0	19.074981552316707	0.0
19	3.081032700540567E-4	0.0	0.0	19.918260202454658	0.0
20	3.081032700540567E-4	0.0	0.0	20.88246938608883	0.0
21	3.081032700540567E-4	0.0	0.0	22.094393598846462	0.0
22	3.081032700540567E-4	0.0	0.0	23.33805244841966	0.0
23	3.081032700540567E-4	0.0	0.0	24.47664808290443	0.0
24	3.081032700540567E-4	0.0	0.0	25.32932388277903	0.0
25	3.081032700540567E-4	0.0	0.0	26.127927558759144	0.0
26	3.081032700540567E-4	0.0	0.0	26.8213139680158	0.0
27	3.081032700540567E-4	0.0	0.0	27.46247687299829	0.0
28	3.081032700540567E-4	0.0	0.0	28.158020005145325	0.0
29	3.081032700540567E-4	0.0	0.0	28.875438469466197	0.0
30	3.081032700540567E-4	0.0	0.0	29.69175808347442	0.0
31	3.081032700540567E-4	0.0	0.0	30.488205036564157	0.0
32	3.081032700540567E-4	0.0	0.0	31.222569180738	0.0
33	3.081032700540567E-4	0.0	0.0	31.93752281889844	0.0
34	3.081032700540567E-4	0.0	0.0	32.6583304191899	0.0
35	3.081032700540567E-4	0.0	0.0	33.351870880081584	0.0
36	3.081032700540567E-4	0.0	0.0	34.05280581945456	0.0
37	3.081032700540567E-4	0.0	0.0	34.793640132299544	0.0
38	3.081032700540567E-4	0.0	0.0	35.55018771191728	0.0
39	3.081032700540567E-4	0.0	0.0	36.34571035519686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTCG	25	3.8889222E-5	45.000004	20
CTATGCG	20	7.031014E-4	45.000004	1
ACTATGC	25	3.8889222E-5	45.000004	8
TCGCACT	20	7.031014E-4	45.000004	29
TCGCAAC	25	3.8889222E-5	45.000004	16
TCCGATA	20	7.031014E-4	45.000004	14
CCGATAG	20	7.031014E-4	45.000004	12
CCGATAA	20	7.031014E-4	45.000004	15
TTTCGCA	25	3.8889222E-5	45.000004	14
AGCTTCG	20	7.031014E-4	45.000004	23
GACCGCG	20	7.031014E-4	45.000004	10
GGCCGAA	25	3.8889222E-5	45.000004	10
CGCGCAT	30	2.1640353E-6	45.000004	36
TATGCGT	20	7.031014E-4	45.000004	31
GCGTTAT	20	7.031014E-4	45.000004	41
GTTCGAA	20	7.031014E-4	45.000004	8
TGATTCC	20	7.031014E-4	45.000004	16
CGTAGAT	20	7.031014E-4	45.000004	30
CGAATCG	20	7.031014E-4	45.000004	38
TCCGTCG	20	7.031014E-4	45.000004	1
>>END_MODULE