SRR2933984.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933984.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354857
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2902	0.8177942100620814	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGC	2531	0.713245053641326	No Hit
GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2503	0.7053545512699482	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	2394	0.674637952752799	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2341	0.6597023589784053	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	2277	0.6416669249866848	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCG	2076	0.5850243901064373	No Hit
TCCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	884	0.2491144320106409	TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	622	0.17528187410703466	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.17415465948255213	No Hit
TGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	576	0.16231890592548548	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	550	0.15499201086634898	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	498	0.140338220748076	TruSeq Adapter, Index 13 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	491	0.13836559515523153	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTC	489	0.13780198784299028	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCAACTAT	482	0.13582936225014583	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	476	0.13413854031342204	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTA	417	0.1175121246023046	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	115	0.0	45.000004	1
GAGTCGG	45	3.8380676E-10	45.000004	15
CTCGTTT	20	7.02669E-4	45.0	45
CGTATGG	25	3.8853374E-5	45.0	2
GTTTCCG	20	7.02669E-4	45.0	1
TTTACCG	20	7.02669E-4	45.0	1
ATGGCGT	20	7.02669E-4	45.0	12
TCCGAAT	25	3.8853374E-5	45.0	37
AACCCAT	20	7.02669E-4	45.0	20
GTGGGCG	50	2.1827873E-11	45.0	5
GCTATCC	20	7.02669E-4	45.0	21
TGGCGAT	20	7.02669E-4	45.0	6
TGAACCG	20	7.02669E-4	45.0	35
ATTCGTT	20	7.02669E-4	45.0	20
CGTCTTA	20	7.02669E-4	45.0	18
GCGCCAC	20	7.02669E-4	45.0	14
TTACTCG	20	7.02669E-4	45.0	1
ATTCGAA	25	3.8853374E-5	45.0	10
TACGGTA	25	3.8853374E-5	45.0	28
TGCGGTG	20	7.02669E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content