##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933984.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354857
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31796188323747	33.0	31.0	34.0	31.0	34.0
2	32.540795306278305	34.0	31.0	34.0	31.0	34.0
3	32.61431788016018	34.0	31.0	34.0	31.0	34.0
4	36.01634179401844	37.0	35.0	37.0	35.0	37.0
5	36.056431182138155	37.0	35.0	37.0	35.0	37.0
6	35.60836618694291	37.0	35.0	37.0	33.0	37.0
7	36.02223712650448	37.0	35.0	37.0	35.0	37.0
8	35.99978864725791	37.0	35.0	37.0	35.0	37.0
9	37.897462921683946	39.0	38.0	39.0	35.0	39.0
10	37.53705013568846	39.0	37.0	39.0	35.0	39.0
11	37.43517529596429	39.0	37.0	39.0	35.0	39.0
12	37.270703410106044	39.0	37.0	39.0	35.0	39.0
13	37.12430641075137	39.0	37.0	39.0	33.0	39.0
14	38.29490189005712	40.0	38.0	41.0	34.0	41.0
15	38.35238701786917	40.0	37.0	41.0	34.0	41.0
16	38.47635808226976	40.0	38.0	41.0	34.0	41.0
17	38.4360460692617	40.0	37.0	41.0	34.0	41.0
18	38.41550821880363	40.0	37.0	41.0	34.0	41.0
19	38.45084921531772	40.0	37.0	41.0	34.0	41.0
20	38.35008750003522	40.0	37.0	41.0	34.0	41.0
21	38.298390619319896	40.0	37.0	41.0	34.0	41.0
22	38.31045463383842	40.0	37.0	41.0	34.0	41.0
23	38.244289953417855	40.0	37.0	41.0	34.0	41.0
24	38.193771011985106	40.0	37.0	41.0	34.0	41.0
25	38.11771220519815	40.0	37.0	41.0	34.0	41.0
26	38.05359060128446	40.0	37.0	41.0	34.0	41.0
27	38.02843398890257	40.0	37.0	41.0	34.0	41.0
28	37.96490980873986	40.0	36.0	41.0	34.0	41.0
29	37.90515616149604	40.0	36.0	41.0	34.0	41.0
30	37.85451040841804	40.0	36.0	41.0	34.0	41.0
31	37.81945121556007	40.0	36.0	41.0	34.0	41.0
32	37.681001079308004	40.0	36.0	41.0	33.0	41.0
33	37.60607512321865	40.0	36.0	41.0	33.0	41.0
34	37.640531256252515	40.0	36.0	41.0	33.0	41.0
35	37.62417255401471	40.0	36.0	41.0	33.0	41.0
36	37.54174498459943	40.0	36.0	41.0	33.0	41.0
37	37.467568626235355	40.0	36.0	41.0	33.0	41.0
38	37.40093615174563	40.0	35.0	41.0	33.0	41.0
39	37.333292002130435	40.0	35.0	41.0	33.0	41.0
40	37.27563779212471	39.0	35.0	41.0	32.0	41.0
41	37.2292641824735	39.0	35.0	41.0	32.0	41.0
42	37.24740388381799	39.0	35.0	41.0	32.0	41.0
43	37.099713405681726	39.0	35.0	41.0	32.0	41.0
44	36.959862705258736	39.0	35.0	41.0	32.0	41.0
45	36.99524597232124	39.0	35.0	41.0	32.0	41.0
46	36.94638403638649	39.0	35.0	41.0	32.0	41.0
47	36.82578616174966	39.0	35.0	41.0	31.0	41.0
48	36.801376329056495	39.0	35.0	41.0	31.0	41.0
49	36.82921007617153	39.0	35.0	41.0	31.0	41.0
50	36.72970520519533	39.0	35.0	41.0	31.0	41.0
51	35.79826239865636	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	8.0
11	6.0
12	5.0
13	2.0
14	2.0
15	5.0
16	14.0
17	12.0
18	23.0
19	79.0
20	154.0
21	278.0
22	384.0
23	611.0
24	853.0
25	1339.0
26	1802.0
27	2238.0
28	2735.0
29	3342.0
30	4247.0
31	5539.0
32	7621.0
33	10863.0
34	20250.0
35	30347.0
36	24496.0
37	39724.0
38	79467.0
39	118339.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.435195022220217	4.276370481630629	55.14164860774904	16.146785888400117
2	17.873115085795124	7.306323392239691	52.68460253003322	22.13595899193196
3	19.06176290731196	7.702821136401424	52.36700980958528	20.868406146701346
4	18.858864274905102	4.315822993487518	54.11109263731587	22.714220094291505
5	20.475008242756942	5.20096827736243	49.676630304601574	24.647393175279056
6	19.945217369250148	8.443682948342571	52.57413549683394	19.036964185573343
7	80.24781813519249	1.4360714315907535	12.88885381998946	5.4272566132273
8	80.84242384960703	4.183093471454699	8.275446165638552	6.699036513299723
9	75.95848468538031	4.921700854146882	10.755881946812378	8.363932513660433
10	37.267969914641675	35.56221238414347	15.508218803630758	11.661598897584097
11	26.97819121505282	23.661925789825197	31.35882904944809	18.001053945673892
12	25.255525465187382	21.022552746599334	33.12376534773162	20.598156440481656
13	22.031973442823446	25.099688043352675	35.15866954857872	17.709668965245154
14	17.720941111489978	30.082540290877734	30.401542029606293	21.794976568025994
15	14.18064177964645	26.40922963334526	39.85267304858014	19.557455538428155
16	17.01445934559555	25.747836452430136	34.427670864601794	22.810033337372516
17	16.804515621785676	25.459269508562603	31.162411901132003	26.573802968519715
18	17.431246952997967	25.569736541761895	33.901825242280694	23.097191262959445
19	20.693687879906552	26.298762600145974	31.073361945797885	21.934187574149586
20	23.131007701693925	26.454600021980685	32.046711774038556	18.367680502286838
21	21.74819716110997	27.983103052779008	30.36462575065449	19.90407403545654
22	19.57323654317091	25.274688113803588	29.72915850610246	25.422916836923044
23	18.993284618874647	27.345944986290256	29.72042259276272	23.940347802072385
24	20.87488763079212	23.981772939522116	30.230487210341067	24.912852219344693
25	17.119008502016307	27.383988479866538	29.127789503941028	26.36921351417613
26	17.343042408632208	27.637329966718987	29.33857863871926	25.681048985929543
27	20.897995530594013	28.289423626982135	28.342402714332813	22.470178128091035
28	15.972349425261442	26.355968742338465	31.9858421843165	25.68583964808359
29	18.2360781948785	25.508303344727594	30.04506040461369	26.210558055780215
30	20.55926753593701	27.964785815131165	28.29139625257498	23.184550396356844
31	20.733140391763445	27.560679372254178	24.599204750082425	27.10697548589995
32	20.39920305926049	30.8304471942219	25.49815841310725	23.272191333410362
33	20.076256069346243	29.744375903532973	25.276097132084193	24.90327089503659
34	17.66007152176792	26.461926917039825	27.696790538160442	28.18121102303181
35	17.308944166241613	26.464744953601027	27.376097977495164	28.850212902662197
36	20.942520508261076	26.842643656458797	26.188013763290563	26.026822071989564
37	20.148679608969246	27.762450790036553	28.891919843768054	23.19694975722615
38	17.155924780968107	30.021670701155678	27.09147628481332	25.730928233062894
39	18.75938758429452	29.895986270525874	28.192201365620516	23.152424779559087
40	20.42146554809402	25.296668798981	29.771710858176675	24.510154794748306
41	16.864257996883254	25.846185928416237	28.046227071750028	29.243329002950485
42	20.155442896716142	23.861724582014727	29.169214641390756	26.81361787987837
43	21.712689900438768	26.024004035428355	26.90858571199103	25.354720352141847
44	19.43994341382585	25.532256655497847	28.160075748822766	26.867724181853536
45	18.97722181047577	25.229035921512043	26.01667714036922	29.777065127642967
46	21.883181112391753	25.535638299371293	27.771186703376287	24.809993884860663
47	16.820860233840673	24.84521934187574	32.19127704962844	26.142643374655144
48	18.119693284900677	24.645984156998452	30.055487139890154	27.178835418210717
49	18.66272893024514	22.95769845317973	32.845906942796674	25.53366567377845
50	19.44501587963602	23.41450217975128	29.360277520240547	27.78020442037215
51	17.983582118994413	22.57134564063834	26.641435845988664	32.803636394378586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	121.0
2	208.0
3	616.5
4	1025.0
5	725.0
6	425.0
7	459.0
8	493.0
9	576.0
10	659.0
11	676.0
12	693.0
13	708.5
14	724.0
15	729.0
16	734.0
17	788.5
18	843.0
19	912.0
20	981.0
21	1031.5
22	1082.0
23	1286.0
24	1490.0
25	1957.0
26	3229.5
27	4035.0
28	4690.5
29	5346.0
30	5720.5
31	6095.0
32	6829.5
33	7564.0
34	8773.0
35	9982.0
36	10750.5
37	11519.0
38	12015.5
39	12512.0
40	15463.5
41	18415.0
42	20821.5
43	23228.0
44	25990.0
45	28752.0
46	37218.0
47	45684.0
48	47708.5
49	49733.0
50	45913.0
51	42093.0
52	33546.5
53	25000.0
54	20497.5
55	15995.0
56	13374.5
57	10754.0
58	9706.0
59	8658.0
60	7774.0
61	6890.0
62	5516.5
63	4143.0
64	3478.0
65	2813.0
66	2217.0
67	1621.0
68	1297.0
69	973.0
70	787.5
71	602.0
72	425.0
73	248.0
74	229.0
75	146.5
76	83.0
77	58.0
78	33.0
79	27.0
80	21.0
81	29.0
82	37.0
83	19.5
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	354857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.66357452521857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.11409028618893	18.950661540852057
2	13.751093048782423	7.883109608139802
3	6.1626868352864825	5.299339001364524
4	3.140629690156459	3.6008669271965514
5	1.9115034950257288	2.7395261442442873
6	1.201057187147753	2.06559553157154
7	0.7763904239592166	1.5577887344474735
8	0.5995292780032876	1.3747721712078176
9	0.49061479207970105	1.2656496290356014
>10	5.3188239064401035	37.59056728913024
>50	0.4476485271621776	7.909522551040873
>100	0.07394194421875255	3.955906918995385
>500	0.004996077312077875	0.9308353233952359
>1k	0.006994508236909025	4.875858629378615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2902	0.8177942100620814	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGC	2531	0.713245053641326	No Hit
GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2503	0.7053545512699482	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	2394	0.674637952752799	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2341	0.6597023589784053	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	2277	0.6416669249866848	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCG	2076	0.5850243901064373	No Hit
TCCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	884	0.2491144320106409	TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	622	0.17528187410703466	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.17415465948255213	No Hit
TGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	576	0.16231890592548548	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	550	0.15499201086634898	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	498	0.140338220748076	TruSeq Adapter, Index 13 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTG	491	0.13836559515523153	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTC	489	0.13780198784299028	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCAACTAT	482	0.13582936225014583	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	476	0.13413854031342204	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTA	417	0.1175121246023046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.818036561206345E-4	0.0	0.0	0.7028183183648624	0.0
2	2.818036561206345E-4	0.0	0.0	3.0575696689088843	0.0
3	2.818036561206345E-4	0.0	0.0	4.239172399022705	0.0
4	2.818036561206345E-4	0.0	0.0	5.5625223681652045	0.0
5	2.818036561206345E-4	0.0	0.0	8.014214176414725	0.0
6	2.818036561206345E-4	0.0	0.0	9.085913480641498	0.0
7	2.818036561206345E-4	0.0	0.0	10.268643425379802	0.0
8	2.818036561206345E-4	0.0	0.0	12.195335022276579	0.0
9	2.818036561206345E-4	0.0	0.0	13.024683182239606	0.0
10	2.818036561206345E-4	0.0	0.0	15.083540693856962	0.0
11	2.818036561206345E-4	0.0	0.0	17.58792978580104	0.0
12	2.818036561206345E-4	0.0	0.0	20.876578452728847	0.0
13	2.818036561206345E-4	0.0	0.0	21.728189101525402	0.0
14	2.818036561206345E-4	0.0	0.0	22.036764104977497	0.0
15	2.818036561206345E-4	0.0	0.0	22.527384270283523	0.0
16	2.818036561206345E-4	0.0	0.0	23.479317020659025	0.0
17	2.818036561206345E-4	0.0	0.0	24.86663641974091	0.0
18	2.818036561206345E-4	0.0	0.0	26.593529224448158	0.0
19	2.818036561206345E-4	0.0	0.0	27.781331634996633	0.0
20	2.818036561206345E-4	0.0	0.0	28.889101807206846	0.0
21	2.818036561206345E-4	0.0	0.0	30.199488808167796	0.0
22	2.818036561206345E-4	0.0	0.0	31.652186655469666	0.0
23	2.818036561206345E-4	0.0	0.0	32.935238701786915	0.0
24	2.818036561206345E-4	0.0	0.0	33.83701040137295	0.0
25	2.818036561206345E-4	0.0	0.0	34.58970796687116	0.0
26	2.818036561206345E-4	0.0	0.0	35.279563317054475	0.0
27	2.818036561206345E-4	0.0	0.0	35.981254420794855	0.0
28	2.818036561206345E-4	0.0	0.0	36.732261164356345	0.0
29	2.818036561206345E-4	0.0	0.0	37.52863829655326	0.0
30	2.818036561206345E-4	0.0	0.0	38.42590113764136	0.0
31	2.818036561206345E-4	0.0	0.0	39.27103030234714	0.0
32	2.818036561206345E-4	0.0	0.0	40.026264100750446	0.0
33	2.818036561206345E-4	0.0	0.0	40.69413876575635	0.0
34	2.818036561206345E-4	0.0	0.0	41.3927300292794	0.0
35	2.818036561206345E-4	0.0	0.0	42.113301977979866	0.0
36	2.818036561206345E-4	0.0	0.0	42.84260984002007	0.0
37	2.818036561206345E-4	0.0	0.0	43.526829117080965	0.0
38	2.818036561206345E-4	0.0	0.0	44.26684551805375	0.0
39	2.818036561206345E-4	0.0	0.0	44.99643518375007	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	115	0.0	45.000004	1
GAGTCGG	45	3.8380676E-10	45.000004	15
CTCGTTT	20	7.02669E-4	45.0	45
CGTATGG	25	3.8853374E-5	45.0	2
GTTTCCG	20	7.02669E-4	45.0	1
TTTACCG	20	7.02669E-4	45.0	1
ATGGCGT	20	7.02669E-4	45.0	12
TCCGAAT	25	3.8853374E-5	45.0	37
AACCCAT	20	7.02669E-4	45.0	20
GTGGGCG	50	2.1827873E-11	45.0	5
GCTATCC	20	7.02669E-4	45.0	21
TGGCGAT	20	7.02669E-4	45.0	6
TGAACCG	20	7.02669E-4	45.0	35
ATTCGTT	20	7.02669E-4	45.0	20
CGTCTTA	20	7.02669E-4	45.0	18
GCGCCAC	20	7.02669E-4	45.0	14
TTACTCG	20	7.02669E-4	45.0	1
ATTCGAA	25	3.8853374E-5	45.0	10
TACGGTA	25	3.8853374E-5	45.0	28
TGCGGTG	20	7.02669E-4	45.0	2
>>END_MODULE