##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933983.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2062189
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56509369412794	34.0	31.0	34.0	31.0	34.0
2	32.79519142037903	34.0	31.0	34.0	31.0	34.0
3	32.843388263636356	34.0	31.0	34.0	31.0	34.0
4	36.16736681264423	37.0	35.0	37.0	35.0	37.0
5	36.20683312732247	37.0	35.0	37.0	35.0	37.0
6	35.867399641836904	37.0	35.0	37.0	35.0	37.0
7	36.18161526416832	37.0	36.0	37.0	35.0	37.0
8	36.1933203018734	37.0	37.0	37.0	35.0	37.0
9	38.0618124720867	39.0	39.0	39.0	37.0	39.0
10	37.71028067747427	39.0	38.0	39.0	35.0	39.0
11	37.63824120873499	39.0	37.0	39.0	35.0	39.0
12	37.58556029539484	39.0	37.0	39.0	35.0	39.0
13	37.6039688893695	39.0	37.0	39.0	35.0	39.0
14	38.926811751978114	40.0	38.0	41.0	35.0	41.0
15	38.974417475798774	40.0	38.0	41.0	35.0	41.0
16	39.014794958173084	40.0	38.0	41.0	35.0	41.0
17	38.97641777742001	40.0	38.0	41.0	35.0	41.0
18	38.909221705672955	40.0	38.0	41.0	35.0	41.0
19	38.9194166974996	40.0	38.0	41.0	35.0	41.0
20	38.889065939154946	40.0	38.0	41.0	35.0	41.0
21	38.8233120242616	40.0	38.0	41.0	35.0	41.0
22	38.80711903710087	40.0	38.0	41.0	35.0	41.0
23	38.7475890910096	40.0	38.0	41.0	35.0	41.0
24	38.72644602410352	40.0	38.0	41.0	35.0	41.0
25	38.65474260603659	40.0	38.0	41.0	35.0	41.0
26	38.58155581277953	40.0	38.0	41.0	35.0	41.0
27	38.5730013107431	40.0	38.0	41.0	35.0	41.0
28	38.4995856344884	40.0	38.0	41.0	35.0	41.0
29	38.465624634793414	40.0	38.0	41.0	34.0	41.0
30	38.40654081657889	40.0	38.0	41.0	34.0	41.0
31	38.37352395924913	40.0	38.0	41.0	34.0	41.0
32	38.297472734070446	40.0	38.0	41.0	34.0	41.0
33	38.23679109916695	40.0	38.0	41.0	34.0	41.0
34	38.235369309020655	40.0	38.0	41.0	34.0	41.0
35	38.18276743790215	40.0	38.0	41.0	34.0	41.0
36	38.07857039291743	40.0	37.0	41.0	34.0	41.0
37	38.02764974500398	40.0	37.0	41.0	34.0	41.0
38	37.99112205525294	40.0	37.0	41.0	34.0	41.0
39	37.947685687393346	40.0	37.0	41.0	34.0	41.0
40	37.865475472907676	40.0	37.0	41.0	33.0	41.0
41	37.782022889269605	40.0	37.0	41.0	33.0	41.0
42	37.75620808761951	40.0	37.0	41.0	33.0	41.0
43	37.671369598033934	40.0	37.0	41.0	33.0	41.0
44	37.56748823701416	40.0	36.0	41.0	33.0	41.0
45	37.55341872156238	40.0	36.0	41.0	33.0	41.0
46	37.484532698021376	40.0	36.0	41.0	33.0	41.0
47	37.424997417792454	40.0	36.0	41.0	33.0	41.0
48	37.366242376426214	40.0	36.0	41.0	33.0	41.0
49	37.322597977198015	39.0	36.0	41.0	33.0	41.0
50	37.256105526700026	39.0	35.0	41.0	33.0	41.0
51	36.40999733778039	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	44.0
11	35.0
12	27.0
13	23.0
14	26.0
15	33.0
16	61.0
17	103.0
18	226.0
19	379.0
20	624.0
21	1150.0
22	1765.0
23	2785.0
24	4154.0
25	6226.0
26	8879.0
27	11242.0
28	13183.0
29	15681.0
30	19860.0
31	24873.0
32	33146.0
33	47089.0
34	85750.0
35	119411.0
36	127279.0
37	209860.0
38	439794.0
39	887860.0
40	605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.436395985043077	4.373120019552039	57.460106711848425	16.730377283556454
2	18.71913777059232	4.174447637922615	55.90103525913484	21.205379332350237
3	20.185249751598906	4.047398177373655	53.75283254832608	22.014519522701363
4	18.502571781732907	4.666546082827519	52.88622914776483	23.944652987674747
5	18.585299407571277	5.316922939652962	51.862753607937975	24.23502404483779
6	21.734768248691076	6.048960594785444	53.651580917171025	18.564690239352455
7	86.86497697349758	1.7620111444683295	7.598721552680186	3.7742903293539047
8	89.15400091844153	2.3287390244056194	5.175713768233658	3.341546288919202
9	83.9207269556767	5.198505083675648	6.918231064175011	3.962536896472632
10	34.96250828609793	40.09923435727763	13.143557646753038	11.794699709871404
11	25.34428221661545	26.518568375643554	29.62691586464674	18.510233543094255
12	25.52302432027326	22.985526544851126	31.112521694180312	20.3789274406953
13	24.2914689196771	22.174252699437346	32.33457263131555	21.199705749569997
14	20.051120435614777	25.57626871251859	31.149909149937276	23.222701701929356
15	17.82479685421656	27.157840527711087	34.25461972690185	20.7627428911705
16	21.544145565707122	26.254916498924203	32.232884570715875	19.968053364652803
17	21.308037236160217	26.179511189323577	29.606064235625347	22.90638733889086
18	20.989637710219576	27.023468750924383	30.27394676239666	21.712946776459383
19	23.160534752149296	26.887690701482747	29.758669064765648	20.193105481602316
20	25.56264241541391	27.041313865993853	29.32592502433094	18.070118694261293
21	25.794240974032935	25.94723374045735	28.861418618759	19.39710666675072
22	23.921619211430183	24.796417787118447	29.116923812511853	22.16503918893952
23	22.441783948997884	26.474101064451418	29.39648111788008	21.687633868670623
24	22.42709082436188	24.411874954235525	30.573870775181128	22.587163446221467
25	22.037359330303865	26.94030469564138	28.041755629576144	22.98058034447861
26	21.65732626834883	29.168422486978642	27.992439102332522	21.18181214234001
27	21.604760766350708	27.983710513439846	29.645342885642396	20.766185834567054
28	20.587831668193363	27.741734632470642	30.86429032450469	20.806143374831308
29	21.319044956597093	25.931376803968988	30.457489589945443	22.29208864948848
30	22.421950655347302	26.36930950557878	29.71289246523961	21.49584737383431
31	22.49216730377284	27.82189217380172	27.713803148014076	21.972137374411364
32	22.25741675472035	28.523961673736014	27.895697242105356	21.32292432943828
33	21.987557881455093	27.794397118789792	28.313699665743535	21.904345334011577
34	22.389509399962858	26.26941565491815	28.982455051404116	22.35861989371488
35	21.989061138431058	25.27479295059764	28.847113431407113	23.88903247956419
36	21.59903869140995	27.115167426458004	29.41224107004741	21.873552812084636
37	22.058016990683203	27.928671911255464	29.554420084677012	20.45889101338432
38	21.347170409695718	27.415188423563503	28.225734886569565	23.011906280171214
39	21.76609418438368	27.2547278644198	28.39599086213727	22.583187089059248
40	22.95449156212161	25.69953578454739	29.561742400914753	21.784230252416243
41	20.508934923035667	25.778481021865602	29.979793316713454	23.73279073838528
42	21.16799187659327	25.692940850717367	29.496617429343285	23.642449843346075
43	21.94430287427583	25.604103212654127	29.87902660716355	22.572567305906492
44	21.781951120872044	25.966388143860726	28.844009933134164	23.407650802133073
45	22.372343175140593	25.151622862889873	27.94297709860735	24.533056863362184
46	22.351734006921774	25.869549299312528	28.3259681823538	23.452748511411905
47	20.726179802142287	25.784154604645842	30.629782236254776	22.8598833569571
48	20.72550091189508	24.53698472836389	31.01272482784071	23.724789531900324
49	21.122118292746205	25.236678112432955	30.727542431852754	22.913661162968086
50	20.728652902328545	25.436805258877826	29.152226105366672	24.682315733426954
51	19.82073418100863	24.549932135221358	28.283925479187406	27.345408204582604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	307.0
1	1226.0
2	2145.0
3	4709.0
4	7273.0
5	5051.0
6	2829.0
7	2784.0
8	2739.0
9	2742.0
10	2745.0
11	2708.5
12	2672.0
13	2786.5
14	2901.0
15	3084.5
16	3268.0
17	3817.5
18	4367.0
19	5028.0
20	5689.0
21	6283.0
22	6877.0
23	9429.5
24	11982.0
25	15173.5
26	23566.0
27	28767.0
28	32165.5
29	35564.0
30	40799.5
31	46035.0
32	53919.5
33	61804.0
34	68462.0
35	75120.0
36	80396.0
37	85672.0
38	92227.0
39	98782.0
40	107404.5
41	116027.0
42	123241.5
43	130456.0
44	139176.5
45	147897.0
46	157925.0
47	167953.0
48	185558.5
49	203164.0
50	193203.5
51	183243.0
52	161015.5
53	138788.0
54	121409.5
55	104031.0
56	94708.0
57	85385.0
58	83033.5
59	80682.0
60	76346.0
61	72010.0
62	61367.5
63	50725.0
64	40603.5
65	30482.0
66	24261.5
67	18041.0
68	14350.5
69	10660.0
70	9206.5
71	7753.0
72	5957.0
73	4161.0
74	3435.0
75	1908.0
76	1107.0
77	879.0
78	651.0
79	439.5
80	228.0
81	143.5
82	59.0
83	44.5
84	30.0
85	23.5
86	17.0
87	15.5
88	14.0
89	11.0
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2062189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.13631827178098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.35160671807775	17.43244059124986
2	12.129996820540928	6.098069214336163
3	4.699216016636163	3.5436296826605234
4	2.303113201550153	2.315671458004204
5	1.3297345671347545	1.6712315648244052
6	0.8655992519212165	1.3054786975264332
7	0.6008640731729573	1.0572457406947906
8	0.47927911429256204	0.9637849886300105
9	0.38203934243661847	0.8642756253445886
>10	6.923367276599111	46.39235345967251
>50	0.849624723034668	12.854589024733713
>100	0.08066424750213001	3.54391608131775
>500	0.0027410023765818098	0.46979076266252684
>1k	0.0019578588404155785	1.1990835881282702
>5k	1.957858840415578E-4	0.2884395202142332
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	5861	0.2842125527776552	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	4671	0.22650688176495945	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	4316	0.20929216478218052	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3661	0.1775297996449404	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	3549	0.1720986776672749	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9396864205948147E-4	0.0	0.0	0.23795103164646889	0.0
2	1.9396864205948147E-4	0.0	0.0	0.9565563583163328	0.0
3	1.9396864205948147E-4	0.0	0.0	1.4253300740135846	0.0
4	1.9396864205948147E-4	0.0	0.0	1.9179619326841526	0.0
5	1.9396864205948147E-4	0.0	0.0	2.709790421731471	0.0
6	1.9396864205948147E-4	0.0	0.0	3.2567335001786937	0.0
7	1.9396864205948147E-4	0.0	0.0	3.737242318720544	0.0
8	1.9396864205948147E-4	0.0	0.0	4.496144630778265	0.0
9	1.9396864205948147E-4	0.0	0.0	4.836753566234715	0.0
10	1.9396864205948147E-4	0.0	0.0	5.515789289924444	0.0
11	1.9396864205948147E-4	0.0	0.0	6.897524911635161	0.0
12	1.9396864205948147E-4	0.0	0.0	8.239739422526258	0.0
13	1.9396864205948147E-4	0.0	0.0	8.634708069919876	0.0
14	1.9396864205948147E-4	0.0	0.0	8.77422001572116	0.0
15	1.9396864205948147E-4	0.0	0.0	8.975510974018386	0.0
16	1.9396864205948147E-4	0.0	0.0	9.458929322191128	0.0
17	1.9396864205948147E-4	0.0	0.0	10.203962876341595	0.0
18	1.9396864205948147E-4	0.0	0.0	10.94506856549036	0.0
19	1.9396864205948147E-4	0.0	0.0	11.53259958228853	0.0
20	1.9396864205948147E-4	0.0	0.0	12.181327705656464	0.0
21	2.4246080257435182E-4	0.0	0.0	13.075862590674278	0.0
22	2.4246080257435182E-4	0.0	0.0	14.110733788222127	0.0
23	2.4246080257435182E-4	0.0	0.0	15.044838276220075	0.0
24	2.4246080257435182E-4	0.0	0.0	15.7199461349081	0.0
25	2.4246080257435182E-4	0.0	0.0	16.323528056836693	0.0
26	3.394451236040925E-4	0.0	0.0	16.858493571636743	0.0
27	3.394451236040925E-4	0.0	0.0	17.38904629982994	0.0
28	3.394451236040925E-4	0.0	0.0	17.951458377481405	0.0
29	3.394451236040925E-4	0.0	0.0	18.548639334222035	0.0
30	3.394451236040925E-4	0.0	0.0	19.241349847176956	0.0
31	3.394451236040925E-4	0.0	0.0	19.880476522762947	0.0
32	3.394451236040925E-4	0.0	0.0	20.49821815556188	0.0
33	4.364294446338333E-4	0.0	0.0	21.059854358645108	0.0
34	4.8492160514870364E-4	0.0	0.0	21.64340901828106	0.0
35	4.8492160514870364E-4	0.0	0.0	22.25741675472035	0.0
36	4.8492160514870364E-4	0.0	0.0	22.8859721393141	0.0
37	5.33413765663574E-4	0.0	0.0	23.514527523907848	0.0
38	5.819059261784443E-4	0.0	0.0	24.095851544160112	0.0
39	5.819059261784443E-4	0.0	0.0	24.67557532311539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	20	7.0345897E-4	45.000004	23
CGCGCAT	25	3.8918883E-5	45.0	36
TTATCCG	60	3.6379788E-12	41.249996	1
TTTCGCG	580	0.0	39.181038	1
TTGGGAC	6070	0.0	39.069195	5
TTTGGGA	17510	0.0	38.832096	4
CGTAACC	140	0.0	38.571426	35
TTTTGCG	1830	0.0	38.360657	1
TACGGGA	440	0.0	38.352272	4
CGTTTTT	2175	0.0	38.172417	1
TCTTACG	95	0.0	37.894737	1
TTGGGAT	11105	0.0	37.807293	5
TCGTTTG	680	0.0	37.72059	1
GCTACGA	215	0.0	37.67442	10
TTCGGGA	3280	0.0	37.66006	4
TTGTTCG	540	0.0	37.5	1
TAGCGCG	30	1.1402365E-4	37.499996	1
CGGCACG	30	1.1402365E-4	37.499996	13
ATATCGC	30	1.1402365E-4	37.499996	45
CGCACGA	30	1.1402365E-4	37.499996	40
>>END_MODULE