##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933980.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1072432 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45817823414445 33.0 31.0 34.0 31.0 34.0 2 32.674640443403405 34.0 31.0 34.0 31.0 34.0 3 32.73466382950154 34.0 31.0 34.0 31.0 34.0 4 36.09719124382711 37.0 35.0 37.0 35.0 37.0 5 36.128718650693 37.0 35.0 37.0 35.0 37.0 6 35.75318155370224 37.0 35.0 37.0 35.0 37.0 7 36.06119921822549 37.0 35.0 37.0 35.0 37.0 8 36.072749600907095 37.0 36.0 37.0 35.0 37.0 9 37.91945969534665 39.0 38.0 39.0 35.0 39.0 10 37.62229493338506 39.0 37.0 39.0 35.0 39.0 11 37.60338371104182 39.0 37.0 39.0 35.0 39.0 12 37.40389507213511 39.0 37.0 39.0 35.0 39.0 13 37.3217388142092 39.0 37.0 39.0 34.0 39.0 14 38.480972220150086 40.0 38.0 41.0 34.0 41.0 15 38.5540258030346 40.0 38.0 41.0 34.0 41.0 16 38.64053664940994 40.0 38.0 41.0 35.0 41.0 17 38.63280096080684 40.0 38.0 41.0 35.0 41.0 18 38.568936771748696 40.0 38.0 41.0 35.0 41.0 19 38.59713249884375 40.0 38.0 41.0 35.0 41.0 20 38.55120790875319 40.0 38.0 41.0 34.0 41.0 21 38.461984536082475 40.0 38.0 41.0 34.0 41.0 22 38.47147511450609 40.0 38.0 41.0 34.0 41.0 23 38.44577185313381 40.0 38.0 41.0 34.0 41.0 24 38.43138492696973 40.0 37.0 41.0 34.0 41.0 25 38.32341164754502 40.0 37.0 41.0 34.0 41.0 26 38.256108545809894 40.0 37.0 41.0 34.0 41.0 27 38.22843126650454 40.0 37.0 41.0 34.0 41.0 28 38.13747072075432 40.0 37.0 41.0 34.0 41.0 29 38.09493748787802 40.0 37.0 41.0 34.0 41.0 30 38.00067696599878 40.0 37.0 41.0 34.0 41.0 31 37.95032225819446 40.0 37.0 41.0 34.0 41.0 32 37.88346300744476 40.0 36.0 41.0 34.0 41.0 33 37.806041781670075 40.0 36.0 41.0 33.0 41.0 34 37.757236822474525 40.0 36.0 41.0 33.0 41.0 35 37.711736501708266 40.0 36.0 41.0 33.0 41.0 36 37.62076010413714 40.0 36.0 41.0 33.0 41.0 37 37.539758231757354 40.0 36.0 41.0 33.0 41.0 38 37.51241663807122 40.0 36.0 41.0 33.0 41.0 39 37.45870134423441 40.0 35.0 41.0 33.0 41.0 40 37.33998053023409 40.0 35.0 41.0 33.0 41.0 41 37.23948371551763 39.0 35.0 41.0 32.0 41.0 42 37.25212134662151 39.0 35.0 41.0 33.0 41.0 43 37.15736662091396 39.0 35.0 41.0 32.0 41.0 44 37.03285522998195 39.0 35.0 41.0 32.0 41.0 45 37.017347486833664 39.0 35.0 41.0 32.0 41.0 46 36.916580258701714 39.0 35.0 41.0 32.0 41.0 47 36.84936574067167 39.0 35.0 41.0 32.0 41.0 48 36.81012502424396 39.0 35.0 41.0 32.0 41.0 49 36.767494815522106 39.0 35.0 41.0 31.0 41.0 50 36.68485367836842 39.0 35.0 41.0 31.0 41.0 51 35.775927051785104 38.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 33.0 10 48.0 11 38.0 12 27.0 13 22.0 14 18.0 15 24.0 16 36.0 17 86.0 18 156.0 19 255.0 20 472.0 21 797.0 22 1184.0 23 1809.0 24 2624.0 25 3849.0 26 5244.0 27 6802.0 28 8164.0 29 9887.0 30 12210.0 31 15723.0 32 21359.0 33 31052.0 34 59746.0 35 77093.0 36 74768.0 37 119001.0 38 229573.0 39 390013.0 40 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.64557193369836 4.777272591642174 54.32978501201009 16.24737046264938 2 19.80423933638683 4.665377385232817 52.80306816655974 22.72731511182061 3 21.27491533262715 4.58406686857535 51.12007101615767 23.020946782639832 4 19.455219538394974 4.941292315037224 50.2906477986483 25.312840347919497 5 19.01687006728632 6.0991279633580495 49.059800528145374 25.82420144121026 6 22.51061139540782 6.808357079982693 51.72178748862398 18.9592440359855 7 84.0334865054381 2.439315499723992 8.691739895862861 4.83545809897504 8 84.73572217166216 3.1575894788667256 7.0853909618511945 5.0212973876199145 9 78.55845405582825 5.975577006281051 9.659167201277096 5.806801736613604 10 35.43394825965655 35.40718665612365 15.600056693571249 13.558808390648544 11 30.33758783773703 24.682031121786743 25.15917093111731 19.821210109358915 12 28.569177346442476 21.779096483506645 28.66251659778895 20.989209572261924 13 26.093962134662153 22.76694466409059 30.368638757515626 20.77045444373163 14 20.609138854491473 25.513598997418953 29.99770614826861 23.879555999820965 15 19.009130648843005 25.1965625792591 33.59653572440956 22.197771047488327 16 22.36617333313441 25.343984513703433 30.29292300117863 21.99691915198353 17 23.03400122338759 25.24066793978546 27.904426574365555 23.820904262461397 18 23.116057708087787 24.411804198308147 29.938774672893015 22.533363420711055 19 24.21589434108643 25.606472018738717 28.050543064735106 22.127090575439748 20 26.49025765736196 26.221336177958136 28.188080922613274 19.100325242066628 21 25.983745356348937 25.8043400420726 29.342839452757847 18.869075148820624 22 23.679170334342878 24.047865039461712 29.0242178525072 23.248746773688215 23 22.584368985632654 25.022845271308576 28.53458307846092 23.85820266459785 24 23.304694376892893 23.50470705834962 29.512080952451996 23.678517612305487 25 22.225371865069302 26.064496396974352 26.546578244588005 25.163553493368347 26 21.77434093723425 26.50303236009369 27.247508466737287 24.475118235934772 27 21.399678487773585 25.8621525653841 29.9262797081773 22.811889238665014 28 19.46715502707864 25.527585898220117 29.626587047010904 25.378672027690335 29 21.34568905068107 24.634475659062765 28.826256583167975 25.19357870708819 30 22.09109761737807 25.969292225521055 28.297085502857055 23.642524654243815 31 22.149842600742982 27.610142181508945 26.1528003640324 24.087214853715665 32 23.08612574037328 26.87713533352231 25.76424425977591 24.272494666328495 33 22.26015262506154 25.962298775120473 27.60230951706029 24.17523908275769 34 20.41984946364898 25.452149879899146 29.011629641786147 25.116371014665734 35 20.828639951064496 25.42669372044102 28.031520879645516 25.713145448848973 36 22.491682456323574 26.1523341340057 27.70982216121861 23.646161248452117 37 20.117825652349055 26.674605009921375 29.071400331209812 24.13616900651976 38 19.98895967296761 26.27551210706133 26.712649380100554 27.0228788398705 39 20.63757888612052 25.580736121264565 28.355737240216627 25.425947752398287 40 22.18835320094887 25.652535545377237 28.635009026213314 23.524102227460574 41 19.38808241454936 25.84807241857759 27.953194235158968 26.810650931714086 42 20.79945395139272 23.301524012711294 29.221433153803687 26.67758888209229 43 21.253095767377324 23.05535455861071 29.589102152863774 26.102447521148193 44 21.123483819953154 23.759921374968297 27.96820684201889 27.148387963059662 45 21.869358616676863 23.39169289987617 26.590497113103673 28.148451370343295 46 21.103156190788784 24.860876960031035 28.509593149029495 25.526373700150685 47 18.283676727289002 25.130730899488267 30.98984364509824 25.595748728124484 48 18.805294881167292 23.522610291375116 30.81304921897146 26.85904560848613 49 20.543866650752683 23.094890864875346 30.696957942321752 25.66428454205022 50 20.089572112730693 23.73120160532323 29.02766795470482 27.15155832724126 51 19.318241156548854 23.343764453130827 27.2205603711937 30.11743401912662 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 119.0 1 644.0 2 1169.0 3 2004.5 4 2840.0 5 2087.5 6 1335.0 7 1312.0 8 1289.0 9 1373.5 10 1458.0 11 1493.0 12 1528.0 13 1631.5 14 1735.0 15 1628.0 16 1521.0 17 1574.0 18 1627.0 19 1876.5 20 2126.0 21 2366.0 22 2606.0 23 3335.5 24 4065.0 25 4918.0 26 7306.5 27 8842.0 28 10739.0 29 12636.0 30 14515.5 31 16395.0 32 19085.0 33 21775.0 34 25912.5 35 30050.0 36 31743.5 37 33437.0 38 36877.5 39 40318.0 40 45218.0 41 50118.0 42 54843.5 43 59569.0 44 65364.5 45 71160.0 46 81145.5 47 91131.0 48 105055.5 49 118980.0 50 115936.5 51 112893.0 52 96991.5 53 81090.0 54 71918.5 55 62747.0 56 57872.0 57 52997.0 58 49178.0 59 45359.0 60 43040.0 61 40721.0 62 36941.0 63 33161.0 64 27639.0 65 22117.0 66 18040.5 67 13964.0 68 11667.5 69 9371.0 70 7553.5 71 5736.0 72 4748.5 73 3761.0 74 3089.5 75 1810.0 76 1202.0 77 934.5 78 667.0 79 554.5 80 442.0 81 287.5 82 133.0 83 83.5 84 34.0 85 21.5 86 9.0 87 7.0 88 5.0 89 4.0 90 3.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1072432.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.124213320599566 #Duplication Level Percentage of deduplicated Percentage of total 1 69.04476660621597 18.72784978098011 2 12.604207053394564 6.837584017065597 3 5.083394171054346 4.136492036651115 4 2.434173593043618 2.6410017518834166 5 1.4811881978082018 2.0088032322652047 6 0.9670725242492187 1.5738648866535903 7 0.6702553375824525 1.2726104129099842 8 0.5092845113081368 1.1051153380479364 9 0.39176910577302365 0.9563785917667227 >10 6.005730153300901 42.86403975117082 >50 0.734626452413239 11.948702916165052 >100 0.06795638531736456 3.255486905596582 >500 0.002090965702071923 0.33348890252444974 >1k 0.003484942836786538 2.3385814763194124 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 4784 0.4460888895519716 No Hit GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 4431 0.41317304966655233 No Hit CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT 4237 0.3950833246303728 No Hit TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 3950 0.3683217210974682 No Hit TCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG 1541 0.14369209423068316 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1358 0.12662807525325614 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 1167 0.1088180882331001 No Hit CGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG 1157 0.10788562817968879 No Hit GCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG 1109 0.10340981992331448 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.4171826278962209 0.0 2 0.0 0.0 0.0 1.7564750026108882 0.0 3 0.0 0.0 0.0 2.5110216778313217 0.0 4 0.0 0.0 0.0 3.2600668387366287 0.0 5 0.0 0.0 0.0 4.484480134871022 0.0 6 0.0 0.0 0.0 5.177484446566309 0.0 7 0.0 0.0 0.0 5.824145493607054 0.0 8 0.0 0.0 0.0 6.7693802497501006 0.0 9 9.324600534113119E-5 0.0 0.0 7.198964596356692 0.0 10 9.324600534113119E-5 0.0 0.0 8.113148432721141 0.0 11 9.324600534113119E-5 0.0 0.0 9.790737314813434 0.0 12 9.324600534113119E-5 0.0 0.0 11.366408313067868 0.0 13 9.324600534113119E-5 0.0 0.0 11.929334447312277 0.0 14 9.324600534113119E-5 0.0 0.0 12.152192400077581 0.0 15 9.324600534113119E-5 0.0 0.0 12.48759828128963 0.0 16 9.324600534113119E-5 0.0 0.0 13.168387366285229 0.0 17 9.324600534113119E-5 0.0 0.0 14.082477956644338 0.0 18 9.324600534113119E-5 0.0 0.0 15.014098796007579 0.0 19 9.324600534113119E-5 0.0 0.0 15.70906127381503 0.0 20 9.324600534113119E-5 0.0 0.0 16.47656914377788 0.0 21 9.324600534113119E-5 0.0 0.0 17.56708117624241 0.0 22 9.324600534113119E-5 0.0 0.0 18.67335178360959 0.0 23 9.324600534113119E-5 0.0 0.0 19.652714577707492 0.0 24 9.324600534113119E-5 0.0 0.0 20.447077297208587 0.0 25 9.324600534113119E-5 0.0 0.0 21.140827576946602 0.0 26 1.8649201068226238E-4 0.0 0.0 21.80324719889 0.0 27 1.8649201068226238E-4 0.0 0.0 22.445712175690392 0.0 28 1.8649201068226238E-4 0.0 0.0 23.085473018335893 0.0 29 1.8649201068226238E-4 0.0 0.0 23.78174094021812 0.0 30 1.8649201068226238E-4 0.0 0.0 24.562769480955435 0.0 31 1.8649201068226238E-4 0.0 0.0 25.26286048905665 0.0 32 1.8649201068226238E-4 0.0 0.0 25.927890999149596 0.0 33 1.8649201068226238E-4 0.0 0.0 26.557488027212916 0.0 34 1.8649201068226238E-4 0.0 0.0 27.226714607546214 0.0 35 1.8649201068226238E-4 0.0 0.0 27.908715890611248 0.0 36 1.8649201068226238E-4 0.0 0.0 28.53141271427932 0.0 37 1.8649201068226238E-4 0.0 0.0 29.19597699434556 0.0 38 1.8649201068226238E-4 0.0 0.0 29.88152162561356 0.0 39 1.8649201068226238E-4 0.0 0.0 30.756075909708027 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGAT 20 7.0330745E-4 45.000004 17 ATGTCGC 20 7.0330745E-4 45.000004 1 TCTAGCG 25 3.8906303E-5 45.0 1 CGTAGCA 25 3.8906303E-5 45.0 21 ATTATCG 35 1.211829E-7 45.0 30 ATCGCTA 35 1.211829E-7 45.0 10 GGTCGAC 30 2.165365E-6 44.999996 9 ACACGCG 180 0.0 41.25 36 CGTTTGG 715 0.0 40.594402 2 GGACCGT 50 1.0822987E-9 40.5 8 TAGTCGG 50 1.0822987E-9 40.5 2 GACCGAT 545 0.0 39.63303 9 CGCTTGG 330 0.0 39.545452 2 TCTTACG 40 3.4579352E-7 39.375004 1 TATCGCG 40 3.4579352E-7 39.375004 32 TTTGGGA 7540 0.0 39.121353 4 GCGGGAT 860 0.0 38.720932 5 TTTACGG 140 0.0 38.571426 2 CTAGTCG 35 6.2478994E-6 38.571426 1 CGTAAGG 35 6.2478994E-6 38.571426 2 >>END_MODULE