##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933978.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 475313 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43348277871634 33.0 31.0 34.0 31.0 34.0 2 32.666649134359886 34.0 31.0 34.0 31.0 34.0 3 32.725200026088075 34.0 31.0 34.0 31.0 34.0 4 36.09596623698489 37.0 35.0 37.0 35.0 37.0 5 36.12547942092895 37.0 35.0 37.0 35.0 37.0 6 35.75503720706145 37.0 35.0 37.0 35.0 37.0 7 36.07689880142138 37.0 35.0 37.0 35.0 37.0 8 36.06861373452862 37.0 36.0 37.0 35.0 37.0 9 37.91035170508697 39.0 38.0 39.0 35.0 39.0 10 37.62123064170347 39.0 37.0 39.0 35.0 39.0 11 37.605308501976594 39.0 37.0 39.0 35.0 39.0 12 37.39274541197064 39.0 37.0 39.0 35.0 39.0 13 37.29330357048934 39.0 37.0 39.0 34.0 39.0 14 38.524056779427454 40.0 38.0 41.0 35.0 41.0 15 38.605340060128796 40.0 38.0 41.0 35.0 41.0 16 38.68995588170321 40.0 38.0 41.0 35.0 41.0 17 38.6613010794992 40.0 38.0 41.0 35.0 41.0 18 38.60958568353906 40.0 38.0 41.0 35.0 41.0 19 38.63433569037666 40.0 38.0 41.0 35.0 41.0 20 38.55827633580399 40.0 38.0 41.0 35.0 41.0 21 38.49918474773465 40.0 38.0 41.0 34.0 41.0 22 38.497817227805676 40.0 38.0 41.0 35.0 41.0 23 38.43825016357642 40.0 37.0 41.0 34.0 41.0 24 38.42477062483037 40.0 37.0 41.0 34.0 41.0 25 38.30451092227648 40.0 37.0 41.0 34.0 41.0 26 38.201460932059504 40.0 37.0 41.0 34.0 41.0 27 38.15659575900512 40.0 37.0 41.0 34.0 41.0 28 38.09836676042944 40.0 37.0 41.0 34.0 41.0 29 38.08922751955028 40.0 37.0 41.0 34.0 41.0 30 38.030558810720514 40.0 37.0 41.0 34.0 41.0 31 37.954775064010455 40.0 37.0 41.0 34.0 41.0 32 37.86853084178215 40.0 36.0 41.0 34.0 41.0 33 37.801002707689456 40.0 36.0 41.0 33.0 41.0 34 37.77032818374418 40.0 36.0 41.0 33.0 41.0 35 37.73056701584009 40.0 36.0 41.0 33.0 41.0 36 37.62533740924401 40.0 36.0 41.0 33.0 41.0 37 37.57606461426471 40.0 36.0 41.0 33.0 41.0 38 37.591791093447895 40.0 36.0 41.0 33.0 41.0 39 37.55785976819485 40.0 36.0 41.0 33.0 41.0 40 37.42983255244439 40.0 36.0 41.0 33.0 41.0 41 37.284574585588864 39.0 35.0 41.0 33.0 41.0 42 37.279371698228324 39.0 35.0 41.0 33.0 41.0 43 37.17954063953648 39.0 35.0 41.0 32.0 41.0 44 37.08802830976641 39.0 35.0 41.0 32.0 41.0 45 37.09784499897962 39.0 35.0 41.0 32.0 41.0 46 37.025902931331565 39.0 35.0 41.0 32.0 41.0 47 36.949997159766305 39.0 35.0 41.0 32.0 41.0 48 36.928994157534085 39.0 35.0 41.0 32.0 41.0 49 36.93849526522523 39.0 35.0 41.0 32.0 41.0 50 36.86214347177544 39.0 35.0 41.0 32.0 41.0 51 35.95846736781868 38.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 9.0 11 6.0 12 7.0 13 4.0 14 5.0 15 8.0 16 8.0 17 16.0 18 59.0 19 113.0 20 198.0 21 336.0 22 530.0 23 777.0 24 1120.0 25 1690.0 26 2332.0 27 2870.0 28 3405.0 29 4046.0 30 5105.0 31 6593.0 32 9138.0 33 13046.0 34 25055.0 35 36997.0 36 33103.0 37 53529.0 38 104687.0 39 170390.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.948246734257218 4.41540626913213 55.268633510970666 17.36771348563999 2 19.265199984010536 5.351000288231123 52.78269266777892 22.601107059979427 3 20.079400310952995 5.3865558063844245 51.6598536122513 22.874190270411287 4 20.544567474485234 5.038574581381111 50.84691561139712 23.569942332736534 5 19.641793933681594 6.085042908567617 48.62711518515168 25.646047972599106 6 20.895704514709255 6.890617340573475 53.15129188555752 19.06238625915975 7 82.10821080004123 2.281864792252684 10.54589291687793 5.064031490828149 8 81.81493037219684 3.8219026199577963 8.52448807417428 5.838678933671075 9 76.0115965689977 6.3800064378630506 11.035254663768928 6.573142329370331 10 34.60414505809856 35.31820084870391 16.637457843568342 13.44019624962919 11 30.681677126440892 24.94608815664625 26.05546240056552 18.316772316347333 12 29.416615998300067 21.68760374742538 28.954815037669913 19.940965216604635 13 24.574543511328326 24.434214927847545 30.294353405019432 20.6968881558047 14 18.53767938179684 28.489647874137674 29.7892125820249 23.183460162040593 15 16.28568964030018 26.68241769107935 36.66952092621073 20.36237174240974 16 19.707434890272303 24.592216076564284 33.30752577775066 22.39282325541275 17 18.867146490838667 24.105168594168475 30.185162198382958 26.842522716609896 18 19.82945974547298 24.850572149299513 31.937481196600974 23.382486908626525 19 23.20029117655103 25.84780975904299 28.724440526558286 22.227458537847692 20 26.0287431650302 25.915764980128884 29.219272353165177 18.83621950167574 21 25.00646942120245 26.227138748571992 28.37225154792737 20.39414028229819 22 21.0192020836796 25.616593697205843 27.638208927590874 25.72599529152369 23 21.249997370153984 26.238710071047922 28.04678180483176 24.464510753966334 24 22.87124484287196 22.29835918647291 29.53148767233381 25.298908298321315 25 19.606028027846914 26.864192647792084 27.158104238680618 26.371675085680383 26 19.64368742281402 31.010302684757203 26.296356295746172 23.049653596682607 27 20.546250575936277 31.563201511425103 26.773094781754338 21.117453130884282 28 19.00347770837322 28.36488797907905 29.81298639002089 22.818647922526843 29 19.930866607898373 26.447625038658735 28.625979091672228 24.995529261770667 30 21.234218294050446 26.80402177091727 27.346401213516145 24.615358721516138 31 21.900726468663805 29.69453812540368 24.043735391205374 24.36100001472714 32 21.821199925101986 29.498036030994314 23.597923894360136 25.08284014954356 33 20.511746996189878 30.153393658494508 24.158186289876355 25.17667305543926 34 19.50840814368637 28.434736689297367 26.3434831363754 25.713372030640862 35 18.922688838723115 27.34787392728581 24.90085480514945 28.828582428841624 36 20.38656632576849 31.256245884290983 24.965443823333256 23.39174396660727 37 20.405290829411356 30.094695495389356 27.137275858223948 22.36273781697534 38 20.637138054292645 27.05459349944142 25.149112269178413 27.159156177087517 39 20.3186111046826 26.242707436994152 26.44615232488907 26.99252913343418 40 21.242213025942906 25.64836223709429 27.56625634055875 25.543168396404052 41 16.921481213431992 29.642572368102705 25.998657726592793 27.437288691872514 42 20.123160948680134 27.977353869976206 25.7129512552781 26.186533926065557 43 21.646157374193425 27.26477079314052 25.693595588591094 25.395476244074956 44 20.855310079884205 25.662458211746785 26.353581745081662 27.128649963287348 45 20.339229097457885 25.131229316261074 25.22653493592643 29.303006650354607 46 19.768868093235405 27.294435456215165 26.97085920225199 25.965837248297436 47 17.812262656396943 27.56709789128427 29.867266411817056 24.75337304050173 48 18.64897446524711 24.44999400395108 29.155104110344134 27.74592742045768 49 20.004923071744305 23.863643535943684 29.835077096565843 26.296356295746172 50 19.159164592594774 24.453149819171788 27.792843873405527 28.59484171482791 51 17.452920496599084 24.59347840265257 25.73924971545066 32.21435138529769 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 80.0 1 271.5 2 463.0 3 1035.5 4 1608.0 5 1150.0 6 692.0 7 737.0 8 782.0 9 777.5 10 773.0 11 849.5 12 926.0 13 878.5 14 831.0 15 903.5 16 976.0 17 913.0 18 850.0 19 915.5 20 981.0 21 1151.0 22 1321.0 23 1392.0 24 1463.0 25 1974.5 26 2990.5 27 3495.0 28 4315.5 29 5136.0 30 6293.0 31 7450.0 32 8606.5 33 9763.0 34 11492.0 35 13221.0 36 14180.0 37 15139.0 38 16620.0 39 18101.0 40 20822.0 41 23543.0 42 26632.5 43 29722.0 44 32395.0 45 35068.0 46 41187.5 47 47307.0 48 53318.5 49 59330.0 50 56315.5 51 53301.0 52 43421.0 53 33541.0 54 28672.5 55 23804.0 56 21783.0 57 19762.0 58 19335.5 59 18909.0 60 17537.0 61 16165.0 62 14026.5 63 11888.0 64 9182.0 65 6476.0 66 5134.0 67 3792.0 68 3135.0 69 2478.0 70 2051.0 71 1624.0 72 1322.0 73 1020.0 74 774.0 75 379.5 76 231.0 77 168.0 78 105.0 79 115.5 80 126.0 81 86.5 82 47.0 83 25.0 84 3.0 85 4.0 86 5.0 87 3.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 475313.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.936489442723712 #Duplication Level Percentage of deduplicated Percentage of total 1 68.17729191380619 19.728114876973457 2 13.037197404540471 7.545014501183807 3 5.544746980359048 4.8133653737920135 4 2.8764340816487226 3.329356177452758 5 1.6381051129781503 2.3700505653881985 6 1.0504575387361241 1.8237932087800435 7 0.7004568105415326 1.4188132772323285 8 0.5248678495258862 1.2150266389304722 9 0.407985295745008 1.0625095982800745 >10 5.454268183317488 40.46296979100647 >50 0.5137530216826312 9.100273750456013 >100 0.064948890525219 3.1502003436431796 >500 0.005838102519120808 1.2824216843373795 >1k 0.0036488140744505054 2.6980902125438333 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC 3781 0.7954758232995941 No Hit CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT 3061 0.6439966927056487 No Hit TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC 2471 0.5198679606911656 No Hit GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC 2369 0.49840841719035667 No Hit TCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG 1095 0.2303745111116254 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCG 993 0.20891496761081646 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGC 955 0.2009202357183582 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 811 0.17062440959956912 No Hit GAATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTC 787 0.16557510524643762 No Hit CGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG 745 0.15673882262845745 No Hit GCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG 618 0.13001958709313652 No Hit TGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG 606 0.12749493491657077 No Hit TTCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT 558 0.11739632621030774 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1038768138047982E-4 0.0 0.0 0.6755548449127207 0.0 2 2.1038768138047982E-4 0.0 0.0 2.6161708179662666 0.0 3 2.1038768138047982E-4 0.0 0.0 3.681784424158397 0.0 4 2.1038768138047982E-4 0.0 0.0 4.6645052838866174 0.0 5 2.1038768138047982E-4 0.0 0.0 6.300269506619848 0.0 6 2.1038768138047982E-4 0.0 0.0 7.222188326429111 0.0 7 2.1038768138047982E-4 0.0 0.0 8.045224935989548 0.0 8 2.1038768138047982E-4 0.0 0.0 9.33574297357741 0.0 9 2.1038768138047982E-4 0.0 0.0 9.912836383604068 0.0 10 2.1038768138047982E-4 0.0 0.0 11.243117692972842 0.0 11 2.1038768138047982E-4 0.0 0.0 13.342576365468648 0.0 12 2.1038768138047982E-4 0.0 0.0 15.437196121292706 0.0 13 2.1038768138047982E-4 0.0 0.0 16.106439335764012 0.0 14 2.1038768138047982E-4 0.0 0.0 16.347122843263282 0.0 15 2.1038768138047982E-4 0.0 0.0 16.734656952366127 0.0 16 2.1038768138047982E-4 0.0 0.0 17.64500444969946 0.0 17 2.1038768138047982E-4 0.0 0.0 18.88965797274638 0.0 18 2.1038768138047982E-4 0.0 0.0 20.272956977823036 0.0 19 2.1038768138047982E-4 0.0 0.0 21.17047082659216 0.0 20 2.1038768138047982E-4 0.0 0.0 22.185381001571596 0.0 21 2.1038768138047982E-4 0.0 0.0 23.400580249225246 0.0 22 2.1038768138047982E-4 0.0 0.0 24.687732083911023 0.0 23 2.1038768138047982E-4 0.0 0.0 25.907980635917806 0.0 24 2.1038768138047982E-4 0.0 0.0 26.728492593301677 0.0 25 2.1038768138047982E-4 0.0 0.0 27.476841575972042 0.0 26 2.1038768138047982E-4 0.0 0.0 28.15407952233581 0.0 27 2.1038768138047982E-4 0.0 0.0 28.833210957831998 0.0 28 2.1038768138047982E-4 0.0 0.0 29.547687523800107 0.0 29 2.1038768138047982E-4 0.0 0.0 30.29540534342633 0.0 30 2.1038768138047982E-4 0.0 0.0 31.212274858882463 0.0 31 2.1038768138047982E-4 0.0 0.0 32.020163555383505 0.0 32 2.1038768138047982E-4 0.0 0.0 32.78239812502498 0.0 33 2.1038768138047982E-4 0.0 0.0 33.466158089511545 0.0 34 2.1038768138047982E-4 0.0 0.0 34.1381363438408 0.0 35 2.1038768138047982E-4 0.0 0.0 34.842724688784024 0.0 36 2.1038768138047982E-4 0.0 0.0 35.60096189247927 0.0 37 2.1038768138047982E-4 0.0 0.0 36.32679939324193 0.0 38 2.1038768138047982E-4 0.0 0.0 37.00214385047327 0.0 39 2.1038768138047982E-4 0.0 0.0 37.72587747442212 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACGA 35 1.209919E-7 45.0 22 GGTCGAA 20 7.029108E-4 45.0 11 CAACGGT 20 7.029108E-4 45.0 12 TCCGATA 35 1.209919E-7 45.0 14 GCCGGTC 20 7.029108E-4 45.0 44 CACGATT 25 3.887341E-5 45.0 10 TTCCGCA 20 7.029108E-4 45.0 22 TCGATAA 25 3.887341E-5 45.0 23 GCGCAAC 20 7.029108E-4 45.0 9 ATTCCGA 35 1.209919E-7 45.0 12 GACCAAT 50 2.1827873E-11 45.0 9 GTATGGA 20 7.029108E-4 45.0 14 TCGTCTA 20 7.029108E-4 45.0 1 GTCGTCA 20 7.029108E-4 45.0 11 AACGAGT 20 7.029108E-4 45.0 30 AATTCCG 35 1.209919E-7 45.0 11 CTCGACC 25 3.887341E-5 45.0 37 CCGAATC 20 7.029108E-4 45.0 40 GTAAGCG 20 7.029108E-4 45.0 20 ACGAGGC 20 7.029108E-4 45.0 29 >>END_MODULE