SRR2933977.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933977.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2871065
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	17261	0.6012054760167395	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	16412	0.5716345676604326	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	12126	0.42235198436817	RNA PCR Primer, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	10696	0.3725446828964165	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8432	0.29368892727959833	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	5545	0.1931339067558554	Illumina PCR Primer Index 7 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	4865	0.16944931584621037	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	4340	0.15116341845273443	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	3320	0.11563653208826688	RNA PCR Primer, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	3220	0.1121535040133191	RNA PCR Primer, Index 14 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	3162	0.11013334772984938	RNA PCR Primer, Index 14 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	2969	0.10341110354520013	RNA PCR Primer, Index 14 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGATA	20	7.035055E-4	45.0	22
CGTTTTT	4535	0.0	40.385887	1
TTTCGCG	855	0.0	40.26316	1
GCGATCG	45	1.9299478E-8	40.0	9
GCCGATG	280	0.0	39.375004	9
TCTTGCG	850	0.0	39.176468	1
CGTTTGG	1775	0.0	38.53521	2
TTTCTCG	990	0.0	38.181816	1
TTTGGGA	20515	0.0	38.134293	4
TGTTTCG	685	0.0	38.102192	1
TTTTGCG	2580	0.0	38.02326	1
CGGCATA	160	0.0	37.96875	6
TTGGGAC	7590	0.0	37.91502	5
GGGCGAT	4650	0.0	37.838707	7
TACGGGA	675	0.0	37.666668	4
GCGATAC	60	1.5643309E-10	37.499996	9
TTCGGGA	3485	0.0	37.446198	4
TTCGTTG	1270	0.0	37.38189	1
TTGGGAT	12905	0.0	37.328556	5
TTCTTCG	905	0.0	37.29282	1