##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933977.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2871065
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34524436054217	33.0	31.0	34.0	31.0	34.0
2	32.570986376135686	34.0	31.0	34.0	31.0	34.0
3	32.62381346294842	34.0	31.0	34.0	31.0	34.0
4	36.02029560459272	37.0	35.0	37.0	35.0	37.0
5	36.06016478205822	37.0	35.0	37.0	35.0	37.0
6	35.73124467749772	37.0	35.0	37.0	35.0	37.0
7	36.060005259372396	37.0	35.0	37.0	35.0	37.0
8	36.076822363826665	37.0	36.0	37.0	35.0	37.0
9	37.92964596761132	39.0	38.0	39.0	35.0	39.0
10	37.55486831541606	39.0	37.0	39.0	35.0	39.0
11	37.48217577797786	39.0	37.0	39.0	35.0	39.0
12	37.27614839789416	39.0	37.0	39.0	34.0	39.0
13	37.19485626413892	39.0	37.0	39.0	34.0	39.0
14	38.100153079083896	40.0	38.0	41.0	33.0	41.0
15	38.23898797136254	40.0	38.0	41.0	33.0	41.0
16	38.38814655885534	40.0	38.0	41.0	33.0	41.0
17	38.40303789708697	40.0	38.0	41.0	34.0	41.0
18	38.37148932538971	40.0	38.0	41.0	34.0	41.0
19	38.402455534792836	40.0	37.0	41.0	34.0	41.0
20	38.32143960516394	40.0	37.0	41.0	34.0	41.0
21	38.23869679021548	40.0	37.0	41.0	34.0	41.0
22	38.30098691600504	40.0	37.0	41.0	34.0	41.0
23	38.26802771793742	40.0	37.0	41.0	34.0	41.0
24	38.229494630041465	40.0	37.0	41.0	34.0	41.0
25	38.12484670322685	40.0	37.0	41.0	34.0	41.0
26	38.0925921217388	40.0	37.0	41.0	34.0	41.0
27	38.105075642662214	40.0	37.0	41.0	34.0	41.0
28	38.04629292614413	40.0	37.0	41.0	34.0	41.0
29	38.044392934329245	40.0	37.0	41.0	34.0	41.0
30	37.962451564140835	40.0	37.0	41.0	34.0	41.0
31	37.842113292454194	40.0	36.0	41.0	33.0	41.0
32	37.78291052275027	40.0	36.0	41.0	33.0	41.0
33	37.72696473259923	40.0	36.0	41.0	33.0	41.0
34	37.64527797176309	40.0	36.0	41.0	33.0	41.0
35	37.61686099060802	40.0	36.0	41.0	33.0	41.0
36	37.54313887007086	40.0	36.0	41.0	33.0	41.0
37	37.477007312617445	40.0	36.0	41.0	33.0	41.0
38	37.462918464054276	40.0	36.0	41.0	33.0	41.0
39	37.4229064824377	40.0	36.0	41.0	33.0	41.0
40	37.30976902299321	40.0	35.0	41.0	32.0	41.0
41	37.16793802996449	39.0	35.0	41.0	32.0	41.0
42	37.15803334302776	39.0	35.0	41.0	32.0	41.0
43	37.08064254901927	39.0	35.0	41.0	32.0	41.0
44	36.9970676386637	39.0	35.0	41.0	32.0	41.0
45	37.02407294854	39.0	35.0	41.0	32.0	41.0
46	36.960857033888125	39.0	35.0	41.0	32.0	41.0
47	36.87926187669036	39.0	35.0	41.0	31.0	41.0
48	36.82345436275389	39.0	35.0	41.0	31.0	41.0
49	36.82782277656549	39.0	35.0	41.0	31.0	41.0
50	36.74821120385641	39.0	35.0	41.0	31.0	41.0
51	35.755196416660716	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	23.0
10	44.0
11	42.0
12	39.0
13	31.0
14	35.0
15	55.0
16	130.0
17	244.0
18	390.0
19	774.0
20	1326.0
21	2198.0
22	3326.0
23	4858.0
24	7370.0
25	11271.0
26	15261.0
27	19401.0
28	23191.0
29	28202.0
30	35704.0
31	47085.0
32	63982.0
33	96067.0
34	184047.0
35	179380.0
36	195946.0
37	313381.0
38	627933.0
39	1008770.0
40	548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.05064496972378	4.380639240142595	56.4667814904922	16.10193429964142
2	20.186411662571206	4.034948703704027	53.57064364617311	22.20799598755166
3	20.77455578330689	4.289105262332967	52.419711849087356	22.516627105272782
4	19.132482197372752	4.386212085062511	52.181577219603184	24.29972849796156
5	18.428353241741306	5.595728414368884	51.15136717559512	24.824551168294693
6	21.149399264732775	5.853611813038019	53.92476311055305	19.072225811676155
7	82.58210803308181	1.9832361858752763	10.1117529557847	5.322902825258223
8	83.93380156840755	2.3393061459771896	8.273863531476994	5.4530287541382725
9	78.30554167181864	4.9698282693007645	10.850120077392884	5.874509981487706
10	36.38440787651969	33.74643207311572	16.55242915085517	13.316730899509416
11	27.036726789536285	23.11128448850862	30.38659870117883	19.465390020776262
12	25.241539289427443	21.677147678648865	32.975150336199285	20.106162695724407
13	25.056555668366965	21.338875992009935	34.15460813321886	19.449960206404242
14	19.41899608681796	23.750733612788284	35.442283612527056	21.387986687866697
15	17.145937134826276	24.86777554670479	36.596628777126256	21.389658541342673
16	22.582700147854542	25.729093559358635	30.60209364817585	21.08611264461097
17	23.13904422226595	25.20573376081698	29.54280031974198	22.11242169717509
18	23.390623340119433	23.63816214540597	32.04103703677904	20.93017747769556
19	22.844449707686866	25.399424952064827	30.79035131562678	20.965774024621524
20	22.414017098184818	29.17628127541522	30.155604279248294	18.254097347151664
21	22.368911884614246	27.13397293338883	32.55892848124302	17.938186700753903
22	20.78517901893548	23.50841934961417	33.40310999576812	22.30329163568223
23	19.827137316640343	24.969828269300763	32.33897525831007	22.864059155748826
24	20.925893353163374	25.685033254210545	29.98148770578165	23.407585686844428
25	21.833291827248775	26.118530928418547	27.473394019292492	24.574783225040186
26	20.030964119586287	25.73066092199236	28.343245450729952	25.895129507691394
27	19.4259969732486	24.406761950704702	32.221144418534585	23.946096657512108
28	17.12437719104235	24.137071086861496	31.796876768725195	26.941674953370963
29	19.614045659015034	24.312406720154367	29.98235846280039	26.091189158030208
30	22.4520169344825	23.35547958684321	29.787065078638065	24.40543840003622
31	20.643350115723607	25.927730650472906	29.93244667048639	23.4964725633171
32	22.152929313686734	25.750444521458064	27.929008921776415	24.167617243078787
33	21.452771010060726	24.954781588016992	27.706478258068003	25.885969143854282
34	18.375689857248094	24.598850949038077	31.835956343726107	25.189502849987722
35	19.228404790556812	25.914355822665108	28.47128156276504	26.38595782401304
36	20.999663887790767	26.002441602680538	28.08160038173988	24.916294127788817
37	17.857066976888365	27.505821010670257	30.428847831727946	24.208264180713428
38	18.650013148430983	25.472289899392734	30.353022310536332	25.52467464163995
39	18.516299700633738	24.38903333780322	28.91470586698664	28.1799610945764
40	20.943726456907104	24.51985587229826	29.997614125768663	24.538803545025974
41	18.97658186073809	25.764585615442353	29.1174877615101	26.141344762309455
42	18.94436385104482	24.821068140219744	31.431959917312913	24.80260809142252
43	19.480506362621536	23.485918988250003	31.28469749030412	25.74887715882434
44	20.37898828483507	21.890100015151173	29.54656199006292	28.184349709950833
45	20.469303202818466	20.860377595073608	28.06307067238116	30.60724852972677
46	20.455963205291418	21.80152661120525	30.947679693772173	26.794830489731165
47	16.804182420112397	23.679192216128857	33.7916069472478	25.72501841651095
48	16.77001391469716	24.794457805727145	32.07537969359802	26.360148585977676
49	20.397483163913044	21.721103492954704	32.238524728628576	25.64288861450368
50	18.822527528983144	22.21036444664262	29.64154416566675	29.325563858707483
51	17.59019736578587	22.239900524718177	28.508828605412972	31.66107350408298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	406.0
1	1435.5
2	2465.0
3	7972.5
4	13480.0
5	8906.0
6	4332.0
7	4472.5
8	4613.0
9	4773.5
10	4934.0
11	5028.0
12	5122.0
13	5046.5
14	4971.0
15	4894.0
16	4817.0
17	4818.5
18	4820.0
19	5549.5
20	6279.0
21	7445.0
22	8611.0
23	10899.5
24	13188.0
25	15489.0
26	22678.5
27	27567.0
28	32530.5
29	37494.0
30	45734.0
31	53974.0
32	59620.0
33	65266.0
34	73308.5
35	81351.0
36	89791.5
37	98232.0
38	103881.0
39	109530.0
40	119659.0
41	129788.0
42	145153.5
43	160519.0
44	179865.0
45	199211.0
46	235702.0
47	272193.0
48	319558.0
49	366923.0
50	357187.0
51	347451.0
52	291826.5
53	236202.0
54	203599.0
55	170996.0
56	149969.0
57	128942.0
58	113343.0
59	97744.0
60	81638.5
61	65533.0
62	55186.5
63	44840.0
64	37583.0
65	30326.0
66	24740.0
67	19154.0
68	15951.5
69	12749.0
70	10137.0
71	7525.0
72	6347.5
73	5170.0
74	4323.0
75	2563.5
76	1651.0
77	1247.5
78	844.0
79	549.0
80	254.0
81	247.0
82	240.0
83	145.0
84	50.0
85	36.5
86	23.0
87	18.0
88	13.0
89	8.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2871065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.31925426629797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.15915791895746	18.73578015151117
2	13.363232454622892	7.568735554042218
3	5.88900991656186	5.003171076115965
4	3.1478363452397087	3.5657751139814966
5	1.8878322570187094	2.6731000849316002
6	1.1785841296649668	2.002597418532342
7	0.8609240846715787	1.7066509640455994
8	0.6487702139986442	1.4698150920502524
9	0.4881707144044593	1.244216752792219
>10	5.924487218619867	39.08546267825575
>50	0.3497974994400049	6.220723997557776
>100	0.09025574231478502	4.745997039556192
>500	0.007214658959698835	1.3311746093522423
>1k	0.003980501495005918	2.1643168117334746
>5k	2.487813434378699E-4	0.49236093876300085
>10k+	4.975626868757398E-4	1.9901217167786887
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	17261	0.6012054760167395	Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	16412	0.5716345676604326	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	12126	0.42235198436817	RNA PCR Primer, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	10696	0.3725446828964165	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8432	0.29368892727959833	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	5545	0.1931339067558554	Illumina PCR Primer Index 7 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	4865	0.16944931584621037	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	4340	0.15116341845273443	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	3320	0.11563653208826688	RNA PCR Primer, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	3220	0.1121535040133191	RNA PCR Primer, Index 14 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	3162	0.11013334772984938	RNA PCR Primer, Index 14 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	2969	0.10341110354520013	RNA PCR Primer, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4381196524634588E-4	0.0	0.0	0.6461713684643156	0.0
2	2.4381196524634588E-4	0.0	0.0	2.157178607938169	0.0
3	2.4381196524634588E-4	0.0	0.0	3.1195044347654965	0.0
4	2.4381196524634588E-4	0.0	0.0	4.121815423893224	0.0
5	2.4381196524634588E-4	0.0	0.0	5.722545466577733	0.0
6	2.4381196524634588E-4	0.0	0.0	6.801552733915812	0.0
7	2.4381196524634588E-4	0.0	0.0	7.798116726719876	0.0
8	2.4381196524634588E-4	0.0	0.0	9.33514218591359	0.0
9	2.4381196524634588E-4	0.0	0.0	10.144005795758718	0.0
10	2.4381196524634588E-4	0.0	0.0	11.54700433462844	0.0
11	2.4381196524634588E-4	0.0	0.0	14.150811632617165	0.0
12	2.4381196524634588E-4	0.0	0.0	16.47635981769831	0.0
13	2.4381196524634588E-4	0.0	0.0	17.221344692648895	0.0
14	2.4381196524634588E-4	0.0	0.0	17.53854405943439	0.0
15	2.4381196524634588E-4	0.0	0.0	17.910043833908322	0.0
16	2.4381196524634588E-4	0.0	0.0	18.71960404936844	0.0
17	2.4381196524634588E-4	0.0	0.0	19.880392815906294	0.0
18	2.7864224599582387E-4	0.0	0.0	21.08492841506549	0.0
19	2.7864224599582387E-4	0.0	0.0	22.05237429316299	0.0
20	2.7864224599582387E-4	0.0	0.0	23.10912501110215	0.0
21	2.7864224599582387E-4	0.0	0.0	24.53110605298034	0.0
22	2.7864224599582387E-4	0.0	0.0	26.11247045956814	0.0
23	2.7864224599582387E-4	0.0	0.0	27.473219867888744	0.0
24	2.7864224599582387E-4	0.0	0.0	28.51050045888895	0.0
25	2.7864224599582387E-4	0.0	0.0	29.418386556904842	0.0
26	3.134725267453018E-4	0.0	0.0	30.247312408461667	0.0
27	3.134725267453018E-4	0.0	0.0	31.15000879464589	0.0
28	3.134725267453018E-4	0.0	0.0	31.99520038731272	0.0
29	3.134725267453018E-4	0.0	0.0	32.95770036554379	0.0
30	3.134725267453018E-4	0.0	0.0	34.04067828488731	0.0
31	3.483028074947798E-4	0.0	0.0	34.9325772840392	0.0
32	3.483028074947798E-4	0.0	0.0	35.77836099147877	0.0
33	3.831330882442578E-4	0.0	0.0	36.619512271578664	0.0
34	4.179633689937358E-4	0.0	0.0	37.5475651021485	0.0
35	4.179633689937358E-4	0.0	0.0	38.49435662376157	0.0
36	4.179633689937358E-4	0.0	0.0	39.34539970359431	0.0
37	4.179633689937358E-4	0.0	0.0	40.190695787103394	0.0
38	4.179633689937358E-4	0.0	0.0	41.181477953303045	0.0
39	4.179633689937358E-4	0.0	0.0	42.87060724852973	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGATA	20	7.035055E-4	45.0	22
CGTTTTT	4535	0.0	40.385887	1
TTTCGCG	855	0.0	40.26316	1
GCGATCG	45	1.9299478E-8	40.0	9
GCCGATG	280	0.0	39.375004	9
TCTTGCG	850	0.0	39.176468	1
CGTTTGG	1775	0.0	38.53521	2
TTTCTCG	990	0.0	38.181816	1
TTTGGGA	20515	0.0	38.134293	4
TGTTTCG	685	0.0	38.102192	1
TTTTGCG	2580	0.0	38.02326	1
CGGCATA	160	0.0	37.96875	6
TTGGGAC	7590	0.0	37.91502	5
GGGCGAT	4650	0.0	37.838707	7
TACGGGA	675	0.0	37.666668	4
GCGATAC	60	1.5643309E-10	37.499996	9
TTCGGGA	3485	0.0	37.446198	4
TTCGTTG	1270	0.0	37.38189	1
TTGGGAT	12905	0.0	37.328556	5
TTCTTCG	905	0.0	37.29282	1
>>END_MODULE