FastQCFastQC Report
Sat 14 Jan 2017
SRR2933976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346879
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC71610.5316735950296946TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC61080.45349285273584333TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT60970.4526761498248915TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC56830.4219384220854286TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG22340.16586493664241556TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.14418518664260116No Hit
GCCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG18510.1374288261974535No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG17660.13111794006737057No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG17530.13015274571806376TruSeq Adapter, Index 16 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC16610.12332213955373868No Hit
TGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG16210.12035231078664083TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC650.045.00000434
TCGAACA253.8911632E-545.00000438
TTTACGT351.2121382E-745.026
ACGGGTA207.033718E-445.05
CTTTACG800.045.01
TCATCGG551.8189894E-1244.99999644
TTTCGCG4700.041.6489371
CTTTGCG4650.040.645161
GATAACG451.9286745E-840.017
AGACACG1800.040.024
CGACCAA1750.039.85714329
TGGGCGA12850.039.747086
ATCGGCG403.4588084E-739.37520
CGTTTGG7050.039.2553182
TCAAGCG1950.039.2307717
TTGGGAC28550.038.93175
CCGATAG356.249091E-638.57142612
AACGGGA356.249091E-638.5714264
TCTTGCG4100.038.4146351
TTTGGGC22700.038.0616724
Produced by FastQC (version 0.11.5)