##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933976.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346879
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.370605674303334	33.0	31.0	34.0	31.0	34.0
2	32.59781316658735	34.0	31.0	34.0	31.0	34.0
3	32.653121030174205	34.0	31.0	34.0	31.0	34.0
4	36.04069482113835	37.0	35.0	37.0	35.0	37.0
5	36.07210670000794	37.0	35.0	37.0	35.0	37.0
6	35.7108983063809	37.0	35.0	37.0	35.0	37.0
7	36.04711559093282	37.0	35.0	37.0	35.0	37.0
8	36.05560633137795	37.0	36.0	37.0	35.0	37.0
9	37.87930021924761	39.0	38.0	39.0	35.0	39.0
10	37.54422483385664	39.0	37.0	39.0	35.0	39.0
11	37.515991414225034	39.0	37.0	39.0	35.0	39.0
12	37.22273938490392	39.0	37.0	39.0	34.0	39.0
13	37.078085707773305	39.0	37.0	39.0	33.0	39.0
14	37.910271078545286	40.0	37.0	41.0	33.0	41.0
15	38.03936062556473	40.0	37.0	41.0	33.0	41.0
16	38.24966830724957	40.0	37.0	41.0	33.0	41.0
17	38.28450068640167	40.0	37.0	41.0	34.0	41.0
18	38.25514392903891	40.0	37.0	41.0	34.0	41.0
19	38.29699921076801	40.0	37.0	41.0	34.0	41.0
20	38.2192201378149	40.0	37.0	41.0	34.0	41.0
21	38.13797453223341	40.0	37.0	41.0	34.0	41.0
22	38.210370048088954	40.0	37.0	41.0	34.0	41.0
23	38.19470865608566	40.0	37.0	41.0	34.0	41.0
24	38.15075222050385	40.0	37.0	41.0	34.0	41.0
25	38.060484275127905	40.0	36.0	41.0	34.0	41.0
26	38.0264648866008	40.0	36.0	41.0	34.0	41.0
27	38.0209677335529	40.0	36.0	41.0	34.0	41.0
28	37.9332144906855	40.0	36.0	41.0	34.0	41.0
29	37.90940611591687	40.0	36.0	41.0	34.0	41.0
30	37.84002571871712	40.0	36.0	41.0	34.0	41.0
31	37.72770234000233	40.0	36.0	41.0	33.0	41.0
32	37.680090787665414	40.0	36.0	41.0	33.0	41.0
33	37.63828673548255	40.0	36.0	41.0	33.0	41.0
34	37.5504659290107	40.0	36.0	41.0	33.0	41.0
35	37.53246356948174	40.0	35.0	41.0	33.0	41.0
36	37.44217632021882	40.0	35.0	41.0	33.0	41.0
37	37.33777421728307	40.0	35.0	41.0	32.0	41.0
38	37.28390746310544	39.0	35.0	41.0	32.0	41.0
39	37.28839413191534	39.0	35.0	41.0	32.0	41.0
40	37.215437318422815	39.0	35.0	41.0	32.0	41.0
41	37.069579375727145	39.0	35.0	41.0	32.0	41.0
42	37.04908532986259	39.0	35.0	41.0	32.0	41.0
43	36.960745545813694	39.0	35.0	41.0	32.0	41.0
44	36.87469846957299	39.0	35.0	41.0	31.0	41.0
45	36.9257052786479	39.0	35.0	41.0	32.0	41.0
46	36.86320894453028	39.0	35.0	41.0	32.0	41.0
47	36.76178112510478	39.0	35.0	41.0	31.0	41.0
48	36.69110068536224	39.0	35.0	41.0	31.0	41.0
49	36.69807310085019	39.0	35.0	41.0	31.0	41.0
50	36.6239216737361	39.0	35.0	41.0	31.0	41.0
51	35.66030207613304	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	15.0
10	34.0
11	29.0
12	14.0
13	16.0
14	32.0
15	32.0
16	64.0
17	137.0
18	237.0
19	384.0
20	697.0
21	1134.0
22	1705.0
23	2325.0
24	3492.0
25	5221.0
26	7068.0
27	8942.0
28	10990.0
29	13281.0
30	17304.0
31	22810.0
32	31233.0
33	48860.0
34	95517.0
35	91753.0
36	91285.0
37	147266.0
38	285491.0
39	459248.0
40	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.878923793451378	4.663299375816239	54.58040403035462	15.877372800377762
2	20.266334243833338	4.433954349277106	52.29504654835364	23.00466485853592
3	21.748056061457635	4.729823540199231	51.08803389168589	22.43408650665724
4	20.794889518657577	4.930212736259159	48.64475576499448	25.630141980088784
5	19.01685303579609	5.861773774778581	49.23293035231821	25.888442837107117
6	22.765519397065365	6.538449259361828	51.580579992709076	19.115451350863736
7	83.05259789483688	2.3827678655617914	9.377828297864916	5.186805941736415
8	83.55910219106542	2.986088579597722	7.817554509350877	5.637254719985982
9	77.33582600961185	5.976706148065268	10.455801894602262	6.23166594772062
10	37.16978288324341	32.53818642951594	17.00004232005993	13.291988367180718
11	32.38100824201729	22.757055385079134	25.69963597323887	19.162300399664705
12	29.857247755737525	20.817979937321766	29.567986433822192	19.75678587311852
13	27.891518094795448	21.794014161628475	30.993875470625053	19.320592272951025
14	21.25781157772896	23.367132459560214	33.98092924457208	21.394126718138747
15	18.18782533546072	24.268698227531946	36.541812590440564	21.001663846566768
16	24.69798697581594	24.960742575984927	29.0438859021486	21.297384546050534
17	24.835341556294217	24.093998050307412	28.163777147019147	22.906883246379223
18	25.239312514338703	23.10727244243915	30.837439740318175	20.815975302903972
19	25.101735196702897	24.900009577697773	28.73858750489094	21.259667720708393
20	24.070090928732277	28.964739965505437	28.57079217954991	18.394376926212377
21	23.729303077707797	26.16790372409103	32.0800903421911	18.022702856010078
22	22.193901605118203	22.95002000922132	32.39882721461987	22.457251171040607
23	21.183194629955622	24.72040918300753	31.6211775519553	22.475218635081546
24	22.383302434739868	25.754058085395943	28.635757183830172	23.226882296034017
25	22.680285311449655	25.8309024047446	26.455011920150213	25.033800363655534
26	20.084803460444476	25.61106083026018	26.89009183453005	27.41404387476529
27	20.97538086197795	24.235584636778803	30.987119110179904	23.80191539106334
28	17.311057637694255	24.81373605201358	31.39116431394357	26.484041996348594
29	19.98353229948644	25.109679488654884	30.244216444090377	24.6625717677683
30	24.091176712978672	22.76447995699688	29.383931295981302	23.760412034043146
31	21.689624680464988	24.15146423695076	30.522860628163333	23.636050454420925
32	23.381684620518993	24.33188133455195	28.578587980063542	23.707846064865514
33	23.863762075138155	24.071650088835003	27.190861242917887	24.87372659310896
34	18.467657451040516	23.318650004937343	32.448794583626295	25.76489796039585
35	18.695072088881034	25.82280962135426	28.869482707800774	26.612635581963932
36	22.959152232680143	25.892452105942702	26.73142873264785	24.416966928729305
37	20.731929148795103	26.78681603915422	29.868236122175784	22.613018689874888
38	19.99875267191782	25.438810761768504	31.05676159476835	23.50567497154533
39	19.828507237843933	22.766707328572203	29.702519676971722	27.70226575661214
40	21.918821215565764	21.908798043476807	29.615058219780693	26.557322521176737
41	19.053381929631392	23.064878136788828	28.694040073384468	29.18769986019531
42	19.8411290101041	23.312636101683967	31.32597657250577	25.52025831570616
43	20.613581472426254	22.80947286281841	30.823555790831993	25.753389873923343
44	20.769720219856424	21.289588745536907	30.109608955221663	27.831082079385006
45	20.47904822927672	20.281777353422246	28.124575407293456	31.11459901000758
46	20.732523114548524	20.988076879957294	30.55664243038907	27.72275757510511
47	17.088914445915336	23.053370050316325	34.31161967778843	25.546095825979915
48	17.566982631698913	24.27255900492917	32.7713922334523	25.389066129919613
49	21.320400718995543	20.947538717286406	33.02137756992276	24.710682993795285
50	19.442726480997923	21.744640758375475	30.36479149203455	28.447841268592057
51	18.056558903954993	21.563184220705793	27.93599128058274	32.44426559475647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	228.0
1	835.0
2	1442.0
3	2548.0
4	3654.0
5	2606.0
6	1558.0
7	1572.5
8	1587.0
9	1639.0
10	1691.0
11	1744.0
12	1797.0
13	1770.5
14	1744.0
15	1773.5
16	1803.0
17	1806.0
18	1809.0
19	2221.5
20	2634.0
21	2942.0
22	3250.0
23	4283.0
24	5316.0
25	6060.5
26	9201.0
27	11597.0
28	14401.5
29	17206.0
30	18683.0
31	20160.0
32	23082.0
33	26004.0
34	30517.5
35	35031.0
36	37766.5
37	40502.0
38	43905.5
39	47309.0
40	52758.5
41	58208.0
42	63467.5
43	68727.0
44	77078.5
45	85430.0
46	100083.0
47	114736.0
48	139004.5
49	163273.0
50	161925.5
51	160578.0
52	138893.0
53	117208.0
54	102413.5
55	87619.0
56	79683.0
57	71747.0
58	65026.0
59	58305.0
60	50496.0
61	42687.0
62	36756.0
63	30825.0
64	25642.0
65	20459.0
66	16392.0
67	12325.0
68	10349.5
69	8374.0
70	6694.5
71	5015.0
72	4283.5
73	3552.0
74	2868.0
75	1666.0
76	1148.0
77	1022.5
78	897.0
79	570.0
80	243.0
81	199.5
82	156.0
83	94.5
84	33.0
85	24.0
86	15.0
87	9.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1346879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.75160975968761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.64198042337944	18.494222343223672
2	13.372911578782748	7.422396469703735
3	5.690751849712879	4.737825737173568
4	2.867678906564832	3.1833082372429953
5	1.750059951046011	2.428349040874345
6	1.1340911109927698	1.8883712366521148
7	0.829964072292341	1.6122987334172747
8	0.6195165791975303	1.3754065876437171
9	0.4952722674003025	1.237014242071996
>10	6.0342286496113235	41.21946698117984
>50	0.46715207550730764	7.897535073488628
>100	0.08721229407693595	4.474448676604459
>500	0.005940156223096688	1.100516252878866
>1k	0.0021600568083987954	1.051885828141453
>5k	0.0010800284041993977	1.8769545597033235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	7161	0.5316735950296946	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	6108	0.45349285273584333	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	6097	0.4526761498248915	TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	5683	0.4219384220854286	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG	2234	0.16586493664241556	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1942	0.14418518664260116	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG	1851	0.1374288261974535	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1766	0.13111794006737057	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG	1753	0.13015274571806376	TruSeq Adapter, Index 16 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1661	0.12332213955373868	No Hit
TGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG	1621	0.12035231078664083	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2273715753233958E-4	0.0	0.0	0.4701238938315914	0.0
2	2.2273715753233958E-4	0.0	0.0	1.983325896387129	0.0
3	2.2273715753233958E-4	0.0	0.0	2.8816248527150545	0.0
4	2.2273715753233958E-4	0.0	0.0	3.753789315892519	0.0
5	2.2273715753233958E-4	0.0	0.0	5.255780214852262	0.0
6	2.2273715753233958E-4	0.0	0.0	6.1067104023449765	0.0
7	2.2273715753233958E-4	0.0	0.0	6.897947031619024	0.0
8	3.7122859588723265E-4	0.0	0.0	8.10822649993058	0.0
9	3.7122859588723265E-4	0.0	0.0	8.661282862083379	0.0
10	4.4547431506467916E-4	0.0	0.0	9.8097898920393	0.0
11	4.4547431506467916E-4	0.0	0.0	12.046887656574942	0.0
12	4.4547431506467916E-4	0.0	0.0	14.113591495598342	0.0
13	4.4547431506467916E-4	0.0	0.0	14.787222905695314	0.0
14	4.4547431506467916E-4	0.0	0.0	15.046711694220491	0.0
15	4.4547431506467916E-4	0.0	0.0	15.443406571785587	0.0
16	4.4547431506467916E-4	0.0	0.0	16.26478696304568	0.0
17	4.4547431506467916E-4	0.0	0.0	17.393024911666156	0.0
18	4.4547431506467916E-4	0.0	0.0	18.55972214282055	0.0
19	4.4547431506467916E-4	0.0	0.0	19.38956654606687	0.0
20	4.4547431506467916E-4	0.0	0.0	20.39967955547603	0.0
21	4.4547431506467916E-4	0.0	0.0	21.733652391937213	0.0
22	4.4547431506467916E-4	0.0	0.0	23.145583233534712	0.0
23	4.4547431506467916E-4	0.0	0.0	24.319408053730143	0.0
24	4.4547431506467916E-4	0.0	0.0	25.215256901325212	0.0
25	4.4547431506467916E-4	0.0	0.0	26.03181132083877	0.0
26	4.4547431506467916E-4	0.0	0.0	26.778871747202235	0.0
27	4.4547431506467916E-4	0.0	0.0	27.59141689788021	0.0
28	4.4547431506467916E-4	0.0	0.0	28.37611990386664	0.0
29	4.4547431506467916E-4	0.0	0.0	29.227050091359356	0.0
30	4.4547431506467916E-4	0.0	0.0	30.274582943233952	0.0
31	4.4547431506467916E-4	0.0	0.0	31.098487688946076	0.0
32	4.4547431506467916E-4	0.0	0.0	31.839014492021928	0.0
33	4.4547431506467916E-4	0.0	0.0	32.60047858790582	0.0
34	4.4547431506467916E-4	0.0	0.0	33.42943204252201	0.0
35	4.4547431506467916E-4	0.0	0.0	34.28815803052836	0.0
36	4.4547431506467916E-4	0.0	0.0	35.028090867850786	0.0
37	4.4547431506467916E-4	0.0	0.0	35.823411011679596	0.0
38	4.4547431506467916E-4	0.0	0.0	36.74739898684292	0.0
39	4.4547431506467916E-4	0.0	0.0	38.484451832718456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGC	65	0.0	45.000004	34
TCGAACA	25	3.8911632E-5	45.000004	38
TTTACGT	35	1.2121382E-7	45.0	26
ACGGGTA	20	7.033718E-4	45.0	5
CTTTACG	80	0.0	45.0	1
TCATCGG	55	1.8189894E-12	44.999996	44
TTTCGCG	470	0.0	41.648937	1
CTTTGCG	465	0.0	40.64516	1
GATAACG	45	1.9286745E-8	40.0	17
AGACACG	180	0.0	40.0	24
CGACCAA	175	0.0	39.857143	29
TGGGCGA	1285	0.0	39.74708	6
ATCGGCG	40	3.4588084E-7	39.375	20
CGTTTGG	705	0.0	39.255318	2
TCAAGCG	195	0.0	39.23077	17
TTGGGAC	2855	0.0	38.9317	5
CCGATAG	35	6.249091E-6	38.571426	12
AACGGGA	35	6.249091E-6	38.571426	4
TCTTGCG	410	0.0	38.414635	1
TTTGGGC	2270	0.0	38.061672	4
>>END_MODULE